(Circular) (Six-base) MAPSORT of: pFN21AB8402.seq Check: 5332 from: 1 to: 7051 pFN21AB8402 7051 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2541 4861 278 Size: 53 83 186 936 1005 2320 2468 Fragments arranged by size: 2468 2320 1005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4394 1937 Size: 2457 4594 Acc65I G'GTAC_C Cuts at: 4372 4372 Size: 7051 AclI AA'CG_TT Cuts at: 1563 5178 5551 1563 Size: 3615 373 3063 Fragments arranged by size: 3615 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2191 2377 3644 3668 3680 3836 4085 5114 Size: 559 186 1267 24 12 156 249 1029 Cuts at: 5114 6498 6766 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1267 1029 559 268 249 186 156 24 12 AfeI AGC'GCT Cuts at: 2915 3134 3218 3638 3890 2915 Size: 219 84 420 252 6076 Fragments arranged by size: 6076 420 252 219 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6854 AflIII A'CryG_T Cuts at: 1203 2645 5950 6927 1203 Size: 1442 3305 977 1327 Fragments arranged by size: 3305 1442 1327 977 AgeI A'CCGG_T Cuts at: 5882 5882 Size: 7051 AhdI GACnn_n'nnGTC Cuts at: 5780 5780 Size: 7051 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7051 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7051 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3260 3292 3512 3544 3680 3712 3260 Size: 32 220 32 136 32 6599 Fragments arranged by size: 6599 220 136 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3165 3409 3424 3622 3844 3928 4093 4794 6366 Size: 244 15 198 222 84 165 701 1572 Cuts at: 6366 3165 Size: 3850 Fragments arranged by size: 3850 1572 701 244 222 198 165 84 15 ApaI G_GGCC'C Cuts at: 2804 3108 2804 Size: 304 6747 ApaLI G'TGCA_C Cuts at: 2896 3011 3593 3761 5108 6264 2896 Size: 115 582 168 1347 1156 3683 Fragments arranged by size: 3683 1347 1156 582 168 115 ApoI r'AATT_y Cuts at: 1493 2320 2663 4360 4586 4738 6847 1493 Size: 827 343 1697 226 152 2109 1697 Fragments arranged by size: 2109 1697 1697 827 343 226 152 AseI AT'TA_AT Cuts at: 160 1032 5605 160 Size: 872 4573 1606 Fragments arranged by size: 4573 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7051 AvaI C'yCGr_G Cuts at: 1870 1943 2417 2706 3016 4187 4376 1870 Size: 73 474 289 310 1171 189 4545 Fragments arranged by size: 4545 1171 474 310 289 189 73 BamHI G'GATC_C Cuts at: 2378 4381 4748 2378 Size: 2003 367 4681 Fragments arranged by size: 4681 2003 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2949 3414 4167 4372 5827 Size: 325 217 627 1162 465 753 205 1455 Cuts at: 5827 618 Size: 1842 Fragments arranged by size: 1842 1455 1162 753 627 465 325 217 205 BanII G_rGCy'C Cuts at: 1391 2205 2426 2449 2804 3108 3499 4370 1391 Size: 814 221 23 355 304 391 871 4072 Fragments arranged by size: 4072 871 814 391 355 304 221 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6080 BbvCI CC'TCA_GC Cuts at: 4163 4163 Size: 7051 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2398 2432 3286 3320 5242 5276 1903 Size: 34 461 34 854 34 1922 34 3678 Fragments arranged by size: 3678 1922 854 461 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4943 6153 4943 Size: 1210 5841 BclI T'GATC_A Cuts at: 1548 1548 Size: 7051 BfrBI ATG'CAT Cuts at: 2266 2266 Size: 7051 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2020 3651 4089 Size: 107 122 71 631 605 348 1631 438 Cuts at: 4089 4192 5662 136 Size: 103 1470 1525 Fragments arranged by size: 1631 1525 1470 631 605 438 348 122 107 103 71 BglII A'GATC_T Cuts at: 7046 7046 Size: 7051 BlpI GC'TnA_GC Cuts at: 3467 3830 4079 4313 4468 3467 Size: 363 249 234 155 6050 Fragments arranged by size: 6050 363 249 234 155 Bme1580I G_kGCm'C Cuts at: 1790 2804 2900 3015 3108 3455 3597 3765 3791 Size: 1014 96 115 93 347 142 168 26 Cuts at: 3791 5112 6268 1790 Size: 1321 1156 2573 Fragments arranged by size: 2573 1321 1156 1014 347 168 142 115 96 93 26 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7051 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2466 2887 2918 Size: 416 53 83 399 24 1049 421 31 Cuts at: 2918 4782 5740 442 Size: 1864 958 1753 Fragments arranged by size: 1864 1753 1049 958 421 416 399 83 53 31 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7051 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2721 2721 Size: 7051 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2986 3201 3500 5711 1239 Size: 1747 215 299 2211 2579 Fragments arranged by size: 2579 2211 1747 299 215 Bpu10I CC'TnA_GC Cuts at: 4163 4163 Size: 7051 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5176 6041 6339 5176 Size: 865 298 5888 Fragments arranged by size: 5888 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3265 3769 5714 882 Size: 617 1766 504 1945 2219 Fragments arranged by size: 2219 1945 1766 617 504 BsaAI yAC'GTr Cuts at: 493 1204 2648 4842 493 Size: 711 1444 2194 2702 Fragments arranged by size: 2702 2194 1444 711 BsaBI GATnn'nnATC Cuts at: 7045 7045 Size: 7051 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2439 2538 4858 5240 Size: 53 83 186 936 906 99 2320 382 Cuts at: 5240 5875 275 Size: 635 1451 Fragments arranged by size: 2320 1451 936 906 635 382 186 99 83 53 BsaWI w'CCGG_w Cuts at: 2640 2724 2808 3363 3874 5484 5882 6156 6303 Size: 84 84 555 511 1610 398 274 147 Cuts at: 6303 6714 2640 Size: 411 2977 Fragments arranged by size: 2977 1610 555 511 411 398 274 147 84 84 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3260 3290 3512 3542 3680 Size: 30 165 30 1857 30 222 30 138 Cuts at: 3680 3710 1178 Size: 30 4519 Fragments arranged by size: 4519 1857 222 165 138 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2242 2439 1186 Size: 1056 197 5798 Fragments arranged by size: 5798 1056 197 BseYI C'CCAG_C Cuts at: 2559 2855 6254 6750 2559 Size: 296 3399 496 2860 Fragments arranged by size: 3399 2860 496 296 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3410 3830 3914 4079 1163 Size: 573 1674 420 84 165 4135 Fragments arranged by size: 4135 1674 573 420 165 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4501 5262 6290 1540 Size: 275 186 2500 761 1028 2301 Fragments arranged by size: 2500 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2558 2900 3015 3333 3585 3597 3753 3765 4002 Size: 342 115 318 252 12 156 12 237 Cuts at: 4002 4254 5112 5197 6268 2558 Size: 252 858 85 1071 3341 Fragments arranged by size: 3341 1071 858 342 318 252 252 237 156 115 85 12 12 BsmI GAATG_Cn' Cuts at: 4562 4655 4562 Size: 93 6958 BsmBI CGTCTCn'nnnn_ Cuts at: 2146 2675 2146 Size: 529 6522 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2205 2426 2449 2558 2804 2900 3015 Size: 399 415 221 23 109 246 96 115 Cuts at: 3015 3108 3333 3455 3499 3585 3597 3753 3765 Size: 93 225 122 44 86 12 156 12 Cuts at: 3765 3791 4002 4254 4370 5112 5197 6268 1391 Size: 26 211 252 116 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 415 399 252 246 225 221 211 156 122 116 115 109 96 93 86 85 44 26 23 12 12 BspEI T'CCGG_A Cuts at: 2808 3874 6714 2808 Size: 1066 2840 3145 Fragments arranged by size: 3145 2840 1066 BspHI T'CATG_A Cuts at: 4940 6670 4940 Size: 1730 5321 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3232 4406 844 Size: 2388 1174 3489 Fragments arranged by size: 3489 2388 1174 BsrBI CCG'CTC Cuts at: 1394 1823 4938 1394 Size: 429 3115 3507 Fragments arranged by size: 3507 3115 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3205 4335 5546 5720 65 Size: 1183 1957 1130 1211 174 1396 Fragments arranged by size: 1957 1396 1211 1183 1130 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2946 2971 3501 5695 5882 1700 Size: 110 1136 25 530 2194 187 2869 Fragments arranged by size: 2869 2194 1136 530 187 110 25 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7051 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7051 BssSI C'ACGA_G Cuts at: 2840 3700 5111 6123 2840 Size: 860 1411 1012 3768 Fragments arranged by size: 3768 1411 1012 860 BstAPI GCAn_nnn'nTGC Cuts at: 794 2904 794 Size: 2110 4941 BstBI TT'CG_AA Cuts at: 5855 5855 Size: 7051 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7051 BstXI CCAn_nnnn'nTGG Cuts at: 2515 2515 Size: 7051 BstYI r'GATC_y Cuts at: 1972 2077 2353 2378 2518 4381 4748 5133 5150 Size: 105 276 25 140 1863 367 385 17 Cuts at: 5150 6602 6688 6700 7046 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1863 1452 385 367 346 276 140 105 86 25 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2693 2992 513 Size: 552 939 689 299 4572 Fragments arranged by size: 4572 939 689 552 299 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6312 Fragments arranged by size: 6312 683 56 BtsI GCAGTG_nn' Cuts at: 3013 3320 4117 4567 5361 5381 3013 Size: 307 797 450 794 20 4683 Fragments arranged by size: 4683 797 794 450 307 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7051 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7016 DraI TTT'AAA Cuts at: 4355 4355 Size: 7051 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6058 809 Size: 507 4742 1802 Fragments arranged by size: 4742 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4498 5387 7016 Size: 54 1425 50 275 18 2668 889 1629 Cuts at: 7016 8 Size: 43 Fragments arranged by size: 2668 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4498 1537 Size: 275 2686 4090 Fragments arranged by size: 4090 2686 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4981 1353 Size: 3628 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2693 2741 3212 3464 3632 Size: 1064 138 435 669 48 471 252 168 Cuts at: 3632 3800 4049 5638 6012 6158 387 Size: 168 249 1589 374 146 1280 Fragments arranged by size: 1589 1280 1064 669 471 435 374 252 249 168 168 146 138 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2191 2377 2986 3201 3500 3644 3668 Size: 393 559 186 609 215 299 144 24 Cuts at: 3668 3680 3836 4085 5114 5711 6498 6766 1239 Size: 12 156 249 1029 597 787 268 1524 Fragments arranged by size: 1524 1029 787 609 597 559 393 299 268 249 215 186 156 144 24 12 EcoNI CCTnn'n_nnAGG Cuts at: 2403 3044 3239 2403 Size: 641 195 6215 Fragments arranged by size: 6215 641 195 EcoO109I rG'GnC_Cy Cuts at: 2504 2652 2800 2801 2997 3104 3105 3609 3861 Size: 148 148 1 196 107 1 504 252 Cuts at: 3861 4090 4194 2504 Size: 229 104 5361 Fragments arranged by size: 5361 504 252 229 196 148 148 107 104 1 1 EcoRI G'AATT_C Cuts at: 2320 4360 4738 2320 Size: 2040 378 4633 Fragments arranged by size: 4633 2040 378 FseI GG_CCGG'CC Cuts at: 2975 2975 Size: 7051 FspI TGC'GCA Cuts at: 3204 5557 3204 Size: 2353 4698 FspAI rTGC'GCAy Cuts at: 3204 3204 Size: 7051 HaeII r_GCGC'y Cuts at: 1678 2917 3086 3136 3150 3220 3640 3844 3892 Size: 1239 169 50 14 70 420 204 48 Cuts at: 3892 6198 1678 Size: 2306 2531 Fragments arranged by size: 2531 2306 1239 420 204 169 70 50 48 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5692 5724 5766 5798 1373 Size: 32 135 32 4120 32 42 32 2626 Fragments arranged by size: 4120 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4395 4641 669 Size: 988 281 2457 246 3079 Fragments arranged by size: 3079 2457 988 281 246 HindIII A'AGCT_T Cuts at: 748 4241 748 Size: 3493 3558 HpaI GTT'AAC Cuts at: 4641 4641 Size: 7051 KpnI G_GTAC'C Cuts at: 4376 4376 Size: 7051 MfeI C'AATT_G Cuts at: 4650 4650 Size: 7051 MluI A'CGCG_T Cuts at: 6927 6927 Size: 7051 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2860 6165 6349 1305 Size: 1555 3305 184 2007 Fragments arranged by size: 3305 2007 1555 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5229 Fragments arranged by size: 5229 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5009 5368 5527 518 Size: 648 3843 359 159 2042 Fragments arranged by size: 3843 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2293 2994 3348 3406 3421 3600 3768 3826 Size: 687 701 354 58 15 179 168 58 Cuts at: 3826 3925 3936 4075 4467 5144 6292 6537 1606 Size: 99 11 139 392 677 1148 245 2120 Fragments arranged by size: 2120 1148 701 687 677 392 354 245 179 168 139 99 58 58 15 11 NaeI GCC'GGC Cuts at: 2973 2973 Size: 7051 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5560 Fragments arranged by size: 5560 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7051 NgoMIV G'CCGG_C Cuts at: 2971 2971 Size: 7051 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7051 NotI GC'GGCC_GC Cuts at: 4498 4498 Size: 7051 NsiI A_TGCA'T Cuts at: 2268 2268 Size: 7051 NspI r_CATG'y Cuts at: 1132 1229 2114 3490 4162 4409 5954 1132 Size: 97 885 1376 672 247 1545 2229 Fragments arranged by size: 2229 1545 1376 885 672 247 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7051 PciI A'CATG_T Cuts at: 5950 5950 Size: 7051 PflMI CCAn_nnn'nTGG Cuts at: 1108 3461 1108 Size: 2353 4698 PfoI T'CCnGG_A Cuts at: 2180 2180 Size: 7051 PmeI GTTT'AAAC Cuts at: 4355 4355 Size: 7051 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3260 3292 3512 3544 3680 3712 5120 5152 6633 Size: 32 220 32 136 32 1408 32 1481 Cuts at: 6633 6665 3260 Size: 32 3646 Fragments arranged by size: 3646 1481 1408 220 136 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2504 2504 Size: 7051 PshAI GACnn'nnGTC Cuts at: 1613 2138 1613 Size: 525 6526 PsiI TTA'TAA Cuts at: 4621 4621 Size: 7051 PspOMI G'GGCC_C Cuts at: 2800 3104 2800 Size: 304 6747 PstI C_TGCA'G Cuts at: 830 3090 3170 3243 3287 4403 830 Size: 2260 80 73 44 1116 3478 Fragments arranged by size: 3478 2260 1116 80 73 44 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7051 PvuII CAG'CTG Cuts at: 2293 3406 3826 3925 4075 2293 Size: 1113 420 99 150 5269 Fragments arranged by size: 5269 1113 420 150 99 SacII CC_GC'GG Cuts at: 2995 2995 Size: 7051 SalI G'TCGA_C Cuts at: 1936 4393 1936 Size: 2457 4594 SbfI CC_TGCA'GG Cuts at: 3170 3287 4403 3170 Size: 117 1116 5818 Fragments arranged by size: 5818 1116 117 ScaI AGT'ACT Cuts at: 5299 7011 5299 Size: 1712 5339 SfcI C'TryA_G Cuts at: 826 1045 3086 3166 3239 3283 3535 4399 5534 Size: 219 2041 80 73 44 252 864 1135 Cuts at: 5534 6215 6406 826 Size: 681 191 1471 Fragments arranged by size: 2041 1471 1135 864 681 252 219 191 80 73 44 SfiI GGCCn_nnn'nGGCC Cuts at: 4192 4192 Size: 7051 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7051 SgrAI Cr'CCGG_yG Cuts at: 1700 2946 1700 Size: 1246 5805 SmaI CCC'GGG Cuts at: 1872 4378 1872 Size: 2506 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5155 6056 6318 820 Size: 197 926 3212 901 262 1553 Fragments arranged by size: 3212 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4842 493 Size: 4349 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7051 SphI G_CATG'C Cuts at: 1132 3490 4409 1132 Size: 2358 919 3774 Fragments arranged by size: 3774 2358 919 SspI AAT'ATT Cuts at: 5 52 4975 5 Size: 47 4923 2081 Fragments arranged by size: 4923 2081 47 StuI AGG'CCT Cuts at: 1517 2398 4111 1517 Size: 881 1713 4457 Fragments arranged by size: 4457 1713 881 StyI C'CwwG_G Cuts at: 513 1065 2004 2404 2567 4490 513 Size: 552 939 400 163 1923 3074 Fragments arranged by size: 3074 1923 939 552 400 163 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2573 3575 5078 5095 5248 5433 5878 Size: 1255 993 1002 1503 17 153 185 445 Cuts at: 5878 325 Size: 1498 Fragments arranged by size: 1503 1498 1255 1002 993 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4261 5297 7009 Size: 274 80 33 51 1443 2284 1036 1712 Cuts at: 7009 96 Size: 138 Fragments arranged by size: 2284 1712 1443 1036 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4256 4288 7011 7043 4256 Size: 32 2723 32 4264 Fragments arranged by size: 4264 2723 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7051 XbaI T'CTAG_A Cuts at: 2080 2146 4387 2080 Size: 66 2241 4744 Fragments arranged by size: 4744 2241 66 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2372 2372 Size: 7051 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7051 XmaI C'CCGG_G Cuts at: 1870 4376 1870 Size: 2506 4545 XmnI GAAnn'nnTTC Cuts at: 3359 4223 5180 3359 Size: 864 957 5230 Fragments arranged by size: 5230 957 864 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2539 4859 276 Size: 53 83 186 936 1005 2320 2468 Fragments arranged by size: 2468 2320 1005 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfoI SrfI SwaI