Restriction map for
pFN21AB8400
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HapII HgaI Hin1I HincII HindII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MslI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AlwI 177 GATCC GGATC(4/5)
AlwNI 134 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 160 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 96 GGACC G^G[AT]CC
BanI 435 GGTGCC G^G[CT][GA]CC
BanII 106 GAGCTC G[GA]GC[CT]^C
BciVI 185 GTATCC GTATCC(6/5)
BclI 453 TGATCA T^GATCA
BglI 438 GCCACATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaJI 59 CCATGG C^C[ACGT][ACGT]GG
BsmAI 523 GAGAC GTCTC(1/5)
BsmI 576 GCATTC GAATGC(1/-1)
BsrI 448 CCAGT ACTGG(1/-1)
BstAPI 133 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 375 GGATG GGATG(2/0)
CfoI 39 GCGC GCG^C
DsaI 59 CCATGG C^C[GA][CT]GG
EcoT14I 59 CCATGG C^C[AT][AT]GG
FbaI 453 TGATCA T^GATCA
FokI 375 GGATG GGATG(9/13)
HaeII 38 GGCGCT [GA]GCGC^[CT]
HhaI 39 GCGC GCG^C
HinP1I 39 GCGC G^CGC
HindIII 119 AAGCTT A^AGCTT
HphI 275 TCACC GGTGA(8/7)
MaeIII 218 GTGAC ^GT[ACGT]AC
MspA1I 100 CAGCTG C[AC]G^C[GT]G
NcoI 59 CCATGG C^CATGG
PvuII 100 CAGCTG CAG^CTG
SacI 106 GAGCTC GAGCT^C
SfcI 414 CTACAG C^T[GA][CT]AG
SphI 333 GCATGC GCATG^C
SspI 538 AATATT AAT^ATT
StyI 59 CCATGG C^C[AT][AT]GG
Tsp45I 218 GTGAC ^GT[GC]AC
XcmI 74 CCAGCCGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 160 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AspHI 106 GAGCTC G[AT]GC[AT]^C
485 GTGCTC
BbvI 133 GCAGC GCAGC(8/12)
315 GCAGC
BmyI 106 GAGCTC G[AGT]GC[ACT]^C
485 GTGCTC
BseRI 51 CTCCTC GAGGAG(10/8)
54 CTCCTC
BsiHKAI 106 GAGCTC G[AT]GC[AT]^C
485 GTGCTC
Bsp1286I 106 GAGCTC G[AGT]GC[ACT]^C
485 GTGCTC
BsrBI 48 CCGCTC CCGCTC(-3/-3)
69 CCGCTC
BssKI 93 CCTGG ^CC[ACGT]GG
198 CCAGG
BstNI 93 CCTGG CC^[AT]GG
198 CCAGG
Cac8I 80 GCCTGC GC[ACGT]^[ACGT]GC
333 GCATGC
Cfr13I 96 GGACC G^G[ACGT]CC
445 GGGCC
EcoRII 93 CCTGG ^CC[AT]GG
198 CCAGG
HaeIII 446 GGCC GG^CC
558 GGCC
MvaI 93 CCTGG CC^[AT]GG
198 CCAGG
NlaIV 435 GGTGCC GG[ACGT]^[ACGT]CC
592 GGTTCC
NspI 333 GCATGC [GA]CATG^[CT]
421 GCATGT
PleI 525 GACTC GAGTC(4/5)
396 GAGTC
Sau96I 96 GGACC G^G[ACGT]CC
445 GGGCC
ScrFI 93 CCTGG CC^[ACGT]GG
198 CCAGG
TfiI 322 GAATC G^A[AT]TC
626 GATTC
TseI 133 GCAGC G^C[AT]GC
315 GCAGC
TspRI 216 CAGTG CA[GC]TG[ACGT][ACGT]^
417 CAGTG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BpmI BsiYI BslI
DdeI DpnII Fnu4HI HinfI ItaI
MboI MboII MnlI MseI MwoI
NdeII NlaIII Sau3AI Tru9I Tsp509I