(Circular) (Six-base) MAPSORT of: pFN21AB8397.seq Check: 3788 from: 1 to: 6091 pFN21AB8397 6091 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2985 3434 1937 Size: 1048 449 4594 Fragments arranged by size: 4594 1048 449 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 3390 4218 4591 1563 Size: 1827 828 373 3063 Fragments arranged by size: 3063 1827 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2561 3099 4154 5538 5806 1632 Size: 929 538 1055 1384 268 1917 Fragments arranged by size: 1917 1384 1055 929 538 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 2747 4990 5967 1203 Size: 1544 2243 977 1327 Fragments arranged by size: 2243 1544 1327 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6091 AlwNI CAG_nnn'CTG Cuts at: 3834 5406 3834 Size: 1572 4519 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 3095 3317 3400 3626 3778 5887 1493 Size: 1602 222 83 226 152 2109 1697 Fragments arranged by size: 2109 1697 1602 226 222 152 83 AseI AT'TA_AT Cuts at: 160 1032 3074 3080 4645 160 Size: 872 2042 6 1565 1606 Fragments arranged by size: 2042 1606 1565 872 6 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 3416 1870 Size: 73 1473 4545 Fragments arranged by size: 4545 1473 73 AvrII C'CTAG_G Cuts at: 2998 2998 Size: 6091 BamHI G'GATC_C Cuts at: 3154 3421 3788 3154 Size: 267 367 5457 Fragments arranged by size: 5457 367 267 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2944 3412 4867 618 Size: 325 217 627 1157 468 1455 1842 Fragments arranged by size: 1842 1455 1157 627 468 325 217 BanII G_rGCy'C Cuts at: 1391 3144 3410 1391 Size: 1753 266 4072 Fragments arranged by size: 4072 1753 266 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2099 2921 3093 928 Size: 971 200 822 172 3926 Fragments arranged by size: 3926 971 822 200 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4282 4316 1903 Size: 34 2345 34 3678 Fragments arranged by size: 3678 2345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4702 136 Size: 107 122 71 631 605 3030 1525 Fragments arranged by size: 3030 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 3508 3508 Size: 6091 Bme1580I G_kGCm'C Cuts at: 1790 4152 5308 1790 Size: 2362 1156 2573 Fragments arranged by size: 2573 2362 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3822 4780 442 Size: 416 53 83 399 24 2405 958 1753 Fragments arranged by size: 2405 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2089 2219 2262 2916 3243 4751 1239 Size: 850 130 43 654 327 1508 2579 Fragments arranged by size: 2579 1508 850 654 327 130 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2115 2804 3261 4216 5081 5379 2115 Size: 689 457 955 865 298 2827 Fragments arranged by size: 2827 955 865 689 457 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4754 882 Size: 617 3255 2219 Fragments arranged by size: 3255 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3898 4280 4915 275 Size: 53 83 186 936 2365 382 635 1451 Fragments arranged by size: 2365 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2795 4524 4922 5196 5343 5754 2795 Size: 1729 398 274 147 411 3132 Fragments arranged by size: 3132 1729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5866 Fragments arranged by size: 5866 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2360 1186 Size: 1174 4917 BseYI C'CCAG_C Cuts at: 3382 5294 5790 3382 Size: 1912 496 3683 Fragments arranged by size: 3683 1912 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2781 1163 Size: 573 1045 4473 Fragments arranged by size: 4473 1045 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3541 4302 5330 1540 Size: 275 186 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4152 4237 5308 4152 Size: 85 1071 4935 Fragments arranged by size: 4935 1071 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3144 3410 4152 4237 5308 1391 Size: 399 1354 266 742 85 1071 2174 Fragments arranged by size: 2174 1354 1071 742 399 266 85 BspEI T'CCGG_A Cuts at: 2795 5754 2795 Size: 2959 3132 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3446 844 Size: 2602 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2725 3978 1394 Size: 429 902 1253 3507 Fragments arranged by size: 3507 1253 902 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3128 4586 4760 65 Size: 1183 1880 1458 174 1396 Fragments arranged by size: 1880 1458 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4735 4922 1700 Size: 110 2925 187 2869 Fragments arranged by size: 2925 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6091 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 3033 1184 Size: 1849 4242 BstYI r'GATC_y Cuts at: 1972 3154 3421 3788 4173 4190 5642 5728 5740 Size: 1182 267 367 385 17 1452 86 12 Cuts at: 5740 6086 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1182 385 367 346 267 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5539 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2025 487 Size: 683 56 799 4553 Fragments arranged by size: 4553 799 683 56 BtsI GCAGTG_nn' Cuts at: 3607 4401 4421 3607 Size: 794 20 5277 Fragments arranged by size: 5277 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3271 3395 3271 Size: 124 5967 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2851 2974 3538 Size: 54 1425 50 275 18 1021 123 564 Cuts at: 3538 4427 6056 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1021 889 564 275 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2039 2337 2391 3118 4021 1353 Size: 686 298 54 727 903 3423 Fragments arranged by size: 3423 903 727 686 298 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4678 5052 5198 387 Size: 1064 138 3089 374 146 1280 Fragments arranged by size: 3089 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2089 2219 2262 2561 2916 3099 3243 Size: 393 457 130 43 299 355 183 144 Cuts at: 3243 4154 4751 5538 5806 1239 Size: 911 597 787 268 1524 Fragments arranged by size: 1524 911 787 597 457 393 355 299 268 183 144 130 43 EcoO109I rG'GnC_Cy Cuts at: 2995 2995 Size: 6091 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 5238 1678 Size: 3560 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3110 3139 3142 3171 4732 Size: 32 135 32 1538 29 3 29 1561 Cuts at: 4732 4764 4806 4838 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1561 1538 135 42 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 3435 3681 669 Size: 988 281 1497 246 3079 Fragments arranged by size: 3079 1497 988 281 246 HindIII A'AGCT_T Cuts at: 748 3254 748 Size: 2506 3585 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2390 2805 5205 5389 1305 Size: 1085 415 2400 184 2007 Fragments arranged by size: 2400 2007 1085 415 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2853 10 Size: 54 1425 343 1021 3248 Fragments arranged by size: 3248 1425 1021 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4049 4408 4567 518 Size: 648 2883 359 159 2042 Fragments arranged by size: 2883 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3507 4184 5332 5577 1606 Size: 1901 677 1148 245 2120 Fragments arranged by size: 2120 1901 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5539 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 3382 3449 4994 1132 Size: 97 2153 67 1545 2229 Fragments arranged by size: 2229 2153 1545 97 67 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2714 2746 4160 4192 5673 5705 2714 Size: 32 1414 32 1481 32 3100 Fragments arranged by size: 3100 1481 1414 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 2222 3661 2222 Size: 1439 4652 PstI C_TGCA'G Cuts at: 830 2334 3443 830 Size: 1504 1109 3478 Fragments arranged by size: 3478 1504 1109 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SfcI C'TryA_G Cuts at: 826 1045 2316 2330 3439 4574 5255 5446 826 Size: 219 1271 14 1109 1135 681 191 1471 Fragments arranged by size: 1471 1271 1135 1109 681 219 191 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2130 2819 3276 4195 5096 5358 Size: 197 926 187 689 457 919 901 262 Cuts at: 5358 820 Size: 1553 Fragments arranged by size: 1553 926 919 901 689 457 262 197 187 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3382 3449 1132 Size: 2250 67 3774 Fragments arranged by size: 3774 2250 67 SspI AAT'ATT Cuts at: 5 52 2642 3078 4015 5 Size: 47 2590 436 937 2081 Fragments arranged by size: 2590 2081 937 436 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 2778 2998 3169 3530 513 Size: 552 1713 220 171 361 3074 Fragments arranged by size: 3074 1713 552 361 220 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4118 4135 4288 4473 4918 325 Size: 1255 2538 17 153 185 445 1498 Fragments arranged by size: 2538 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4337 6049 96 Size: 274 80 33 51 1443 2360 1712 138 Fragments arranged by size: 2360 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2602 2634 2790 2822 6051 6083 2602 Size: 32 156 32 3229 32 2610 Fragments arranged by size: 3229 2610 156 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 2918 4220 2918 Size: 1302 4789 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI