(Circular) (Six-base) MAPSORT of: pFN21AB7636.seq Check: 7843 from: 1 to: 5836 pFN21AB7636 5836 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2757 3179 1937 Size: 820 422 4594 Fragments arranged by size: 4594 820 422 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2340 3143 3899 5283 5551 1632 Size: 708 803 756 1384 268 1917 Fragments arranged by size: 1917 1384 803 756 708 268 AflII C'TTAA_G Cuts at: 820 1017 2867 820 Size: 197 1850 3789 Fragments arranged by size: 3789 1850 197 AflIII A'CryG_T Cuts at: 1203 2643 4735 5712 1203 Size: 1440 2092 977 1327 Fragments arranged by size: 2092 1440 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AlwNI CAG_nnn'CTG Cuts at: 2044 2318 3579 5151 2044 Size: 274 1261 1572 2729 Fragments arranged by size: 2729 1572 1261 274 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 2507 2609 3145 3371 3523 5632 1493 Size: 1014 102 536 226 152 2109 1697 Fragments arranged by size: 2109 1697 1014 536 226 152 102 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2069 3161 1870 Size: 73 126 1092 4545 Fragments arranged by size: 4545 1092 126 73 AvrII C'CTAG_G Cuts at: 2659 2971 2659 Size: 312 5524 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2665 2698 2665 Size: 33 5803 BamHI G'GATC_C Cuts at: 2818 3166 3533 2818 Size: 348 367 5121 Fragments arranged by size: 5121 367 348 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3157 4612 618 Size: 325 217 627 1370 1455 1842 Fragments arranged by size: 1842 1455 1370 627 325 217 BanII G_rGCy'C Cuts at: 1391 3081 3155 1391 Size: 1690 74 4072 Fragments arranged by size: 4072 1690 74 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2568 928 Size: 971 669 4196 Fragments arranged by size: 4196 971 669 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4027 4061 1903 Size: 34 2090 34 3678 Fragments arranged by size: 3678 2090 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 2269 1548 Size: 721 5115 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2259 2936 4447 Size: 107 122 71 631 605 587 677 1511 Cuts at: 4447 136 Size: 1525 Fragments arranged by size: 1525 1511 677 631 605 587 122 107 71 BglII A'GATC_T Cuts at: 2730 5831 2730 Size: 3101 2735 BlpI GC'TnA_GC Cuts at: 3253 3253 Size: 5836 Bme1580I G_kGCm'C Cuts at: 1790 2694 3897 5053 1790 Size: 904 1203 1156 2573 Fragments arranged by size: 2573 1203 1156 904 BmgBI CAC'GTC Cuts at: 1318 2644 1318 Size: 1326 4510 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3567 4525 442 Size: 416 53 83 399 24 2150 958 1753 Fragments arranged by size: 2150 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2439 2829 4496 1239 Size: 986 214 390 1667 2579 Fragments arranged by size: 2579 1667 986 390 214 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3961 4826 5124 3961 Size: 865 298 4673 Fragments arranged by size: 4673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4499 882 Size: 617 3000 2219 Fragments arranged by size: 3000 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2527 3627 493 Size: 711 1323 1100 2702 Fragments arranged by size: 2702 1323 1100 711 BsaBI GATnn'nnATC Cuts at: 2226 2268 2271 2535 5830 2226 Size: 42 3 264 3295 2232 Fragments arranged by size: 3295 2232 264 42 3 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3643 4025 4660 275 Size: 53 83 186 936 2110 382 635 1451 Fragments arranged by size: 2110 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2275 4269 4667 4941 5088 5499 2275 Size: 1994 398 274 147 411 2612 Fragments arranged by size: 2612 1994 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2541 2571 2597 2627 1178 Size: 30 165 30 1138 30 26 30 4387 Fragments arranged by size: 4387 1138 165 30 30 30 30 26 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2213 2925 2928 1186 Size: 838 189 712 3 4094 Fragments arranged by size: 4094 838 712 189 3 BseYI C'CCAG_C Cuts at: 2311 2794 2813 5039 5535 2311 Size: 483 19 2226 496 2612 Fragments arranged by size: 2612 2226 496 483 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2525 2588 1163 Size: 573 789 63 4411 Fragments arranged by size: 4411 789 573 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4047 5075 1540 Size: 275 186 1285 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2831 3897 3982 5053 2831 Size: 1066 85 1071 3614 Fragments arranged by size: 3614 1071 1066 85 BsmI GAATG_Cn' Cuts at: 2128 2567 3347 3440 2128 Size: 439 780 93 4524 Fragments arranged by size: 4524 780 439 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2694 2831 3081 3155 3897 3982 5053 Size: 399 904 137 250 74 742 85 1071 Cuts at: 5053 1391 Size: 2174 Fragments arranged by size: 2174 1071 904 742 399 250 137 85 74 BspEI T'CCGG_A Cuts at: 2275 5499 2275 Size: 3224 2612 BspHI T'CATG_A Cuts at: 2589 3004 3725 5455 2589 Size: 415 721 1730 2970 Fragments arranged by size: 2970 1730 721 415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3191 844 Size: 2347 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3079 4331 4505 65 Size: 1183 1831 1252 174 1396 Fragments arranged by size: 1831 1396 1252 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2023 3060 4480 4667 1700 Size: 110 213 1037 1420 187 2869 Fragments arranged by size: 2869 1420 1037 213 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5836 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstXI CCAn_nnnn'nTGG Cuts at: 2552 2782 2552 Size: 230 5606 BstYI r'GATC_y Cuts at: 1972 2103 2536 2730 2818 2994 3166 3533 3918 Size: 131 433 194 88 176 172 367 385 Cuts at: 3918 3935 5387 5473 5485 5831 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 433 385 367 346 194 176 172 131 88 86 17 12 BstZ17I GTA'TAC Cuts at: 2758 2758 Size: 5836 BtgI C'CryG_G Cuts at: 513 1065 2004 2193 2545 513 Size: 552 939 189 352 3804 Fragments arranged by size: 3804 939 552 352 189 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2277 487 Size: 683 56 1051 4046 Fragments arranged by size: 4046 1051 683 56 BtsI GCAGTG_nn' Cuts at: 2150 2787 3352 4146 4166 2150 Size: 637 565 794 20 3820 Fragments arranged by size: 3820 794 637 565 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 2205 3062 Size: 54 1425 50 275 18 267 108 857 Cuts at: 3062 3283 4172 5801 8 Size: 221 889 1629 43 Fragments arranged by size: 1629 1425 889 857 275 267 221 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3766 1353 Size: 2413 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 4423 4797 4943 387 Size: 1064 138 453 2381 374 146 1280 Fragments arranged by size: 2381 1280 1064 453 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2340 2439 2829 3143 3899 4496 Size: 393 593 115 99 390 314 756 597 Cuts at: 4496 5283 5551 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 756 597 593 393 390 314 268 115 99 EcoNI CCTnn'n_nnAGG Cuts at: 2663 2663 Size: 5836 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 4983 1678 Size: 3305 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2435 2467 2986 3018 4477 Size: 32 135 32 863 32 519 32 1459 Cuts at: 4477 4509 4551 4583 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1459 863 519 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3180 3426 669 Size: 988 281 1242 246 3079 Fragments arranged by size: 3079 1242 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3115 4950 5134 1305 Size: 1810 1835 184 2007 Fragments arranged by size: 2007 1835 1810 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2099 2207 10 Size: 54 1425 343 267 108 3639 Fragments arranged by size: 3639 1425 343 267 108 54 MslI CAynn'nnrTG Cuts at: 518 1166 2220 2550 2594 3794 4153 4312 518 Size: 648 1054 330 44 1200 359 159 2042 Fragments arranged by size: 2042 1200 1054 648 359 330 159 44 MspA1I CmG'CkG Cuts at: 1606 2369 2794 3252 3929 5077 5322 1606 Size: 763 425 458 677 1148 245 2120 Fragments arranged by size: 2120 1148 763 677 458 425 245 NaeI GCC'GGC Cuts at: 3062 3062 Size: 5836 NcoI C'CATG_G Cuts at: 513 1065 2004 2193 2545 513 Size: 552 939 189 352 3804 Fragments arranged by size: 3804 939 552 352 189 NdeI CA'TA_TG Cuts at: 387 2146 3022 387 Size: 1759 876 3201 Fragments arranged by size: 3201 1759 876 NgoMIV G'CCGG_C Cuts at: 3060 3060 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3131 3194 4739 1132 Size: 97 1902 63 1545 2229 Fragments arranged by size: 2229 1902 1545 97 63 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5836 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2963 1613 Size: 1350 4486 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PstI C_TGCA'G Cuts at: 830 2235 3188 830 Size: 1405 953 3478 Fragments arranged by size: 3478 1405 953 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2369 2794 2369 Size: 425 5411 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SfcI C'TryA_G Cuts at: 826 1045 2062 2231 2346 2677 3184 4319 5000 Size: 219 1017 169 115 331 507 1135 681 Cuts at: 5000 5191 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1017 681 507 331 219 191 169 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2867 3940 4841 5103 820 Size: 197 926 924 1073 901 262 1553 Fragments arranged by size: 1553 1073 926 924 901 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 2490 2723 3760 5 Size: 47 2438 233 1037 2081 Fragments arranged by size: 2438 2081 1037 233 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5836 StyI C'CwwG_G Cuts at: 513 1065 2004 2193 2545 2659 2971 3275 513 Size: 552 939 189 352 114 312 304 3074 Fragments arranged by size: 3074 939 552 352 312 304 189 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3863 3880 4033 4218 4663 325 Size: 1255 2283 17 153 185 445 1498 Fragments arranged by size: 2283 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2743 2871 4082 Size: 274 80 33 51 1443 766 128 1211 Cuts at: 4082 5794 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1211 766 274 138 128 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2597 2629 2810 2842 5796 5828 2597 Size: 32 181 32 2954 32 2605 Fragments arranged by size: 2954 2605 181 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2596 1316 Size: 1280 4556 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2200 2200 Size: 5836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI