Restriction map for
pFN21AB8372
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbrPI BbvCI BcgI BciVI
BclI BfaI BglI BglII BlnI
BsaAI BsaBI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
Ksp632I MaeI MamI MfeI MluI
MluNI MroI MscI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RcaI RsrII SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
AccI 145 GTCGAC GT^[AC][GT]AC
AcsI 13 GAATTC [GA]^AATT[CT]
AcyI 343 GGCGCC G[GA]^CG[CT]C
AfaI 120 GTAC GT^AC
AflII 417 CTTAAG C^TTAAG
ApoI 13 GAATTC [GA]^AATT[CT]
AseI 478 ATTAAT AT^TAAT
AsnI 478 ATTAAT AT^TAAT
AvaI 282 CCCGGG C^[CT]CG[GA]G
AvaII 360 GGACC G^G[AT]CC
BamHI 408 GGATCC G^GATCC
BanI 343 GGCGCC G^G[CT][GA]CC
BanII 298 GAGCTC G[GA]GC[CT]^C
BbeI 343 GGCGCC GGCGC^C
BbsI 33 GAAGAC GAAGAC(2/6)
BfrI 417 CTTAAG C^TTAAG
BlpI 78 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 78 GCTCAGC GC^T[ACGT]AGC
BpuAI 33 GAAGAC GAAGAC(2/6)
BsaHI 343 GGCGCC G[GA]^CG[CT]C
BsaI 313 GAGACC GGTCTC(1/5)
BsgI 364 CTGCAC GTGCAG(16/14)
BsmAI 313 GAGAC GTCTC(1/5)
BsoBI 282 CCCGGG C^[CT]CG[GA]G
BspMI 362 ACCTGC ACCTGC(4/8)
BsrFI 287 GCCGGC [GA]^CCGG[CT]
BsrI 472 CCAGT ACTGG(1/-1)
BstAPI 366 GCACTGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 78 GCTCAGC GC^T[ACGT]AGC
Cfr10I 287 GCCGGC [GA]^CCGG[CT]
DraIII 367 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 329 CCGCGG C^C[GA][CT]GG
EaeI 128 CGGCCA [CT]^GGCC[GA]
EcoNI 228 CCTGATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 13 GAATTC G^AATTC
FauI 328 CCCGC CCCGC(4/6)
HaeII 343 GGCGCC [GA]GCGC^[CT]
Hin1I 343 GGCGCC G[GA]^CG[CT]C
KasI 343 GGCGCC G^GCGCC
KspI 329 CCGCGG CCGC^GG
MaeIII 379 GTGAC ^GT[ACGT]AC
MboII 33 GAAGA GAAGA(8/7)
MslI 395 CATCTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 329 CCGCGG C[AC]G^C[GT]G
NaeI 287 GCCGGC GCC^GGC
NarI 343 GGCGCC GG^CGCC
NgoMIV 287 GCCGGC G^CCGGC
NlaIII 456 CATG CATG^
PleI 93 GAGTC GAGTC(4/5)
PshBI 478 ATTAAT AT^TAAT
PstI 211 CTGCAG CTGCA^G
RsaI 120 GTAC GT^AC
SacI 298 GAGCTC GAGCT^C
SacII 329 CCGCGG CCGC^GG
SalI 145 GTCGAC G^TCGAC
SfaNI 61 GATGC GCATC(5/9)
SfcI 211 CTGCAG C^T[GA][CT]AG
SfoI 343 GGCGCC GGC^GCC
SmaI 282 CCCGGG CCC^GGG
SmlI 417 CTTAAG C^T[CT][GA]AG
TaqI 146 TCGA T^CGA
TfiI 384 GAATC G^A[AT]TC
Tsp45I 379 GTGAC ^GT[GC]AC
Tsp509I 14 AATT ^AATT
TthHB8I 146 TCGA T^CGA
XmaCI 282 CCCGGG C^CCGGG
XmaI 282 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 271 CGCG CG^CG
330 CGCG
AluI 134 AGCT AG^CT
299 AGCT
AspHI 76 GTGCTC G[AT]GC[AT]^C
298 GAGCTC
BmyI 76 GTGCTC G[AGT]GC[ACT]^C
298 GAGCTC
BpmI 23 CTGGAG CTGGAG(16/14)
67 CTGGAG
BsiHKAI 76 GTGCTC G[AT]GC[AT]^C
298 GAGCTC
Bsp1286I 76 GTGCTC G[AGT]GC[ACT]^C
298 GAGCTC
BstNI 66 CCTGG CC^[AT]GG
234 CCAGG
BstUI 271 CGCG CG^CG
330 CGCG
BstYI 215 AGATCC [GA]^GATC[CT]
408 GGATCC
DdeI 79 CTCAG C^T[ACGT]AG
91 CTGAG
EcoRII 66 CCTGG ^CC[AT]GG
234 CCAGG
HgaI 319 GACGC GACGC(5/10)
272 GCGTC
HincII 145 GTCGAC GT[CT]^[GA]AC
422 GTCAAC
HindII 145 GTCGAC GT[CT]^[GA]AC
422 GTCAAC
HinfI 93 GAGTC G^A[ACGT]TC
384 GAATC
HphI 378 GGTGA GGTGA(8/7)
224 TCACC
MaeII 143 ACGT A^CGT
158 ACGT
MflI 215 AGATCC [GA]^GATC[CT]
408 GGATCC
MseI 418 TTAA T^TAA
479 TTAA
MvaI 66 CCTGG CC^[AT]GG
234 CCAGG
MvnI 271 CGCG CG^CG
330 CGCG
Tru9I 418 TTAA T^TAA
479 TTAA
XhoII 215 AGATCC [GA]^GATC[CT]
408 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AlwI BbvI BcnI BsaJI
BsiYI BslI BssKI BstF5I Cac8I
CfoI Cfr13I DpnII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MboI MnlI MspI MwoI
NciI NdeII NlaIV Sau3AI Sau96I
ScrFI TseI TspRI