Restriction map for
pFN21AB8368
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaHI BseAI BseRI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
CelII CfoI ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SwaI
Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcsI 506 AAATTC [GA]^AATT[CT]
AfaI 273 GTAC GT^AC
AgeI 238 ACCGGT A^CCGGT
Alw44I 1334 GTGCAC G^TGCAC
AlwNI 809 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1129 CCTGAGG CC^T[ACGT]AGG
ApaI 1323 GGGCCC GGGCC^C
ApaLI 1334 GTGCAC G^TGCAC
ApoI 506 AAATTC [GA]^AATT[CT]
AspHI 1334 GTGCAC G[AT]GC[AT]^C
BalI 1775 TGGCCA TGG^CCA
BanI 1680 GGTGCC G^G[CT][GA]CC
BanII 1323 GGGCCC G[GA]GC[CT]^C
BbsI 421 GAAGAC GAAGAC(2/6)
BglII 95 AGATCT A^GATCT
BpuAI 421 GAAGAC GAAGAC(2/6)
BsaBI 838 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 452 GGTCTC GGTCTC(1/5)
BsaWI 238 ACCGGT [AT]^CCGG[AT]
BsgI 1803 GTGCAG GTGCAG(16/14)
BsiHKAI 1334 GTGCAC G[AT]GC[AT]^C
BspHI 299 TCATGA T^CATGA
BsrDI 1565 CATTGC GCAATG(2/0)
BstEII 789 GGTGACC G^GT[ACGT]ACC
BstPI 789 GGTGACC G^GT[ACGT]ACC
Bsu36I 1129 CCTGAGG CC^T[ACGT]AGG
DsaI 633 CCATGG C^C[GA][CT]GG
EarI 1405 GAAGAG CTCTTC(1/4)
Eco81I 1129 CCTGAGG CC^T[ACGT]AGG
EcoNI 606 CCTTGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 789 GGTGACC G^GT[ACGT]ACC
EcoT22I 214 ATGCAT ATGCA^T
FauI 1840 CCCGC CCCGC(4/6)
HincII 1543 GTCAAC GT[CT]^[GA]AC
HindII 1543 GTCAAC GT[CT]^[GA]AC
HindIII 1440 AAGCTT A^AGCTT
Ksp632I 1405 GAAGAG CTCTTC(1/4)
MamI 838 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1775 TGGCCA TGG^CCA
MscI 1775 TGGCCA TGG^CCA
NcoI 633 CCATGG C^CATGG
NsiI 214 ATGCAT ATGCA^T
PinAI 238 ACCGGT A^CCGGT
PpuMI 1175 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1328 CTGCAG CTGCA^G
RcaI 299 TCATGA T^CATGA
RsaI 273 GTAC GT^AC
SbfI 1327 CCTGCAGG CCTGCA^GG
SmaI 1609 CCCGGG CCC^GGG
Sse8387I 1327 CCTGCAGG CCTGCA^GG
SspI 1294 AATATT AAT^ATT
StuI 145 AGGCCT AGG^CCT
TaqI 510 TCGA T^CGA
TthHB8I 510 TCGA T^CGA
XbaI 1399 TCTAGA T^CTAGA
XcmI 314 CCACTAACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1609 CCCGGG C^CCGGG
XmaI 1609 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 520 CCCGAG C^[CT]CG[GA]G
1609 CCCGGG
AvaII 963 GGACC G^G[AT]CC
1176 GGACC
BciVI 637 GGATAC GTATCC(6/5)
1464 GGATAC
BfaI 680 CTAG C^TAG
1400 CTAG
BglI 257 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1726 GCCAAAGAGGC
BmyI 1323 GGGCCC G[AGT]GC[ACT]^C
1334 GTGCAC
BpmI 1810 CTCCAG CTGGAG(16/14)
642 CTGGAG
BsoBI 520 CCCGAG C^[CT]CG[GA]G
1609 CCCGGG
Bsp1286I 1323 GGGCCC G[AGT]GC[ACT]^C
1334 GTGCAC
BsrFI 238 ACCGGT [GA]^CCGG[CT]
1338 ACCGGC
Cfr10I 238 ACCGGT [GA]^CCGG[CT]
1338 ACCGGC
DraI 100 TTTAAA TTT^AAA
1374 TTTAAA
EaeI 1340 CGGCCA [CT]^GGCC[GA]
1775 TGGCCA
MaeI 680 CTAG C^TAG
1400 CTAG
MaeII 359 ACGT A^CGT
1703 ACGT
NspI 1692 ACATGC [GA]CATG^[CT]
1843 GCATGT
PflMI 628 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
808 CCAGTTCCTGG
PvuII 530 CAGCTG CAG^CTG
1813 CAGCTG
SfcI 780 CTATAG C^T[GA][CT]AG
1328 CTGCAG
Tsp509I 175 AATT ^AATT
507 AATT
Van91I 628 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
808 CCAGTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwI BamHI BbvI
BcnI BsaJI BsiYI BslI BsmAI
BsmFI BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI Tru9I TseI Tsp45I TspRI
XhoII