(Circular) (Six-base) MAPSORT of: pFN21AB8368.seq Check: 5590 from: 1 to: 6571 pFN21AB8368 6571 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2069 2147 3326 2069 Size: 78 1179 5314 Fragments arranged by size: 5314 1179 78 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4381 278 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3914 1937 Size: 1977 4594 Acc65I G'GTAC_C Cuts at: 3892 3892 Size: 6571 AclI AA'CG_TT Cuts at: 1563 4698 5071 1563 Size: 3135 373 3063 Fragments arranged by size: 3135 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2892 4634 6018 6286 1632 Size: 1260 1742 1384 268 1917 Fragments arranged by size: 1917 1742 1384 1260 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6374 AflIII A'CryG_T Cuts at: 1203 5470 6447 1203 Size: 4267 977 1327 Fragments arranged by size: 4267 1327 977 AgeI A'CCGG_T Cuts at: 2243 5402 2243 Size: 3159 3412 AhdI GACnn_n'nnGTC Cuts at: 5300 5300 Size: 6571 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6571 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6571 AlwNI CAG_nnn'CTG Cuts at: 2819 4314 5886 2819 Size: 1495 1572 3504 Fragments arranged by size: 3504 1572 1495 ApaI G_GGCC'C Cuts at: 3332 3332 Size: 6571 ApaLI G'TGCA_C Cuts at: 3339 4628 5784 3339 Size: 1289 1156 4126 Fragments arranged by size: 4126 1289 1156 ApoI r'AATT_y Cuts at: 1493 2511 3880 4106 4258 6367 1493 Size: 1018 1369 226 152 2109 1697 Fragments arranged by size: 2109 1697 1369 1018 226 152 AseI AT'TA_AT Cuts at: 160 1032 5125 160 Size: 872 4093 1606 Fragments arranged by size: 4093 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 AvaI C'yCGr_G Cuts at: 1870 1943 2525 3614 3896 1870 Size: 73 582 1089 282 4545 Fragments arranged by size: 4545 1089 582 282 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2635 2668 2697 2730 2635 Size: 33 29 33 6476 Fragments arranged by size: 6476 33 33 29 BamHI G'GATC_C Cuts at: 3034 3233 3841 3901 4268 3034 Size: 199 608 60 367 5337 Fragments arranged by size: 5337 608 367 199 60 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3685 3892 5347 618 Size: 325 217 627 1898 207 1455 1842 Fragments arranged by size: 1898 1842 1455 627 325 217 207 BanII G_rGCy'C Cuts at: 1391 3332 3890 1391 Size: 1941 558 4072 Fragments arranged by size: 4072 1941 558 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2433 928 Size: 971 534 5066 Fragments arranged by size: 5066 971 534 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4762 4796 1903 Size: 34 2825 34 3678 Fragments arranged by size: 3678 2825 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2636 3463 4463 5673 2636 Size: 827 1000 1210 3534 Fragments arranged by size: 3534 1210 1000 827 BclI T'GATC_A Cuts at: 1548 1548 Size: 6571 BfrBI ATG'CAT Cuts at: 2221 2221 Size: 6571 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2268 3737 5182 Size: 107 122 71 631 605 596 1469 1445 Cuts at: 5182 136 Size: 1525 Fragments arranged by size: 1525 1469 1445 631 605 596 122 107 71 BglII A'GATC_T Cuts at: 2100 6566 2100 Size: 4466 2105 BlpI GC'TnA_GC Cuts at: 3988 3988 Size: 6571 Bme1580I G_kGCm'C Cuts at: 1790 3332 3343 4632 5788 1790 Size: 1542 11 1289 1156 2573 Fragments arranged by size: 2573 1542 1289 1156 11 BmgBI CAC'GTC Cuts at: 1318 2365 1318 Size: 1047 5524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3181 4302 5260 Size: 416 53 83 399 24 1764 1121 958 Cuts at: 5260 442 Size: 1753 Fragments arranged by size: 1764 1753 1121 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6571 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2231 2231 Size: 6571 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2668 3800 5231 1239 Size: 1429 1132 1431 2579 Fragments arranged by size: 2579 1431 1429 1132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4696 5561 5859 4696 Size: 865 298 5408 Fragments arranged by size: 5408 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2463 5234 882 Size: 617 964 2771 2219 Fragments arranged by size: 2771 2219 964 617 BsaAI yAC'GTr Cuts at: 493 1204 4362 493 Size: 711 3158 2702 Fragments arranged by size: 3158 2702 711 BsaBI GATnn'nnATC Cuts at: 2847 6565 2847 Size: 3718 2853 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4378 4760 5395 275 Size: 53 83 186 936 2845 382 635 1451 Fragments arranged by size: 2845 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2243 5004 5402 5676 5823 6234 2243 Size: 2761 398 274 147 411 2580 Fragments arranged by size: 2761 2580 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6346 Fragments arranged by size: 6346 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6571 BseYI C'CCAG_C Cuts at: 2558 3266 3426 3820 5774 6270 2558 Size: 708 160 394 1954 496 2859 Fragments arranged by size: 2859 1954 708 496 394 160 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3829 1163 Size: 573 2093 3905 Fragments arranged by size: 3905 2093 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4021 4782 5810 1540 Size: 275 186 2020 761 1028 2301 Fragments arranged by size: 2301 2020 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3343 4632 4717 5788 3343 Size: 1289 85 1071 4126 Fragments arranged by size: 4126 1289 1071 85 BsmI GAATG_Cn' Cuts at: 4082 4175 4082 Size: 93 6478 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3332 3343 3890 4632 4717 5788 1391 Size: 399 1542 11 547 742 85 1071 2174 Fragments arranged by size: 2174 1542 1071 742 547 399 85 11 BspEI T'CCGG_A Cuts at: 6234 6234 Size: 6571 BspHI T'CATG_A Cuts at: 2304 4460 6190 2304 Size: 2156 1730 2685 Fragments arranged by size: 2685 2156 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2069 2147 2807 3326 3356 3926 844 Size: 1225 78 660 519 30 570 3489 Fragments arranged by size: 3489 1225 660 570 519 78 30 BsrBI CCG'CTC Cuts at: 1394 1823 4458 1394 Size: 429 2635 3507 Fragments arranged by size: 3507 2635 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3569 5066 5240 65 Size: 1183 2321 1497 174 1396 Fragments arranged by size: 2321 1497 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2243 3343 5215 5402 1700 Size: 110 433 1100 1872 187 2869 Fragments arranged by size: 2869 1872 1100 433 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6571 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6571 BssSI C'ACGA_G Cuts at: 4631 5643 4631 Size: 1012 5559 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6571 BstBI TT'CG_AA Cuts at: 5375 5375 Size: 6571 BstEII G'GTnAC_C Cuts at: 1184 2794 1184 Size: 1610 4961 BstXI CCAn_nnnn'nTGG Cuts at: 2558 3031 3824 2558 Size: 473 793 5305 Fragments arranged by size: 5305 793 473 BstYI r'GATC_y Cuts at: 1972 2100 2547 2718 3034 3233 3284 3691 3841 Size: 128 447 171 316 199 51 407 150 Cuts at: 3841 3901 4268 4653 4670 6122 6208 6220 6566 Size: 60 367 385 17 1452 86 12 346 Cuts at: 6566 1972 Size: 1977 Fragments arranged by size: 1977 1452 447 407 385 367 346 316 199 171 150 128 86 60 51 17 12 Bsu36I CC'TnA_GG Cuts at: 3135 3135 Size: 6571 BtgI C'CryG_G Cuts at: 513 1065 2638 513 Size: 552 1573 4446 Fragments arranged by size: 4446 1573 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5832 Fragments arranged by size: 5832 683 56 BtsI GCAGTG_nn' Cuts at: 2775 3789 4087 4881 4901 2775 Size: 1014 298 794 20 4445 Fragments arranged by size: 4445 1014 794 298 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6571 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6536 DraI TTT'AAA Cuts at: 2107 3381 3875 2107 Size: 1274 494 4803 Fragments arranged by size: 4803 1274 494 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5578 809 Size: 507 4262 1802 Fragments arranged by size: 4262 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3345 3780 4018 Size: 54 1425 50 275 18 1515 435 238 Cuts at: 4018 4907 6536 8 Size: 889 1629 43 Fragments arranged by size: 1629 1515 1425 889 435 275 238 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4018 1537 Size: 275 2206 4090 Fragments arranged by size: 4090 2206 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3405 4501 1353 Size: 2052 1096 3423 Fragments arranged by size: 3423 2052 1096 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2799 5158 5532 5678 387 Size: 1064 138 1210 2359 374 146 1280 Fragments arranged by size: 2359 1280 1210 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2668 2892 3800 4634 5231 6018 6286 Size: 393 1036 224 908 834 597 787 268 Cuts at: 6286 1239 Size: 1524 Fragments arranged by size: 1524 1036 908 834 787 597 393 268 224 EcoNI CCTnn'n_nnAGG Cuts at: 2615 2615 Size: 6571 EcoO109I rG'GnC_Cy Cuts at: 3181 3329 3739 3181 Size: 148 410 6013 Fragments arranged by size: 6013 410 148 EcoRI G'AATT_C Cuts at: 3880 4258 3880 Size: 378 6193 FspI TGC'GCA Cuts at: 5077 5077 Size: 6571 HaeII r_GCGC'y Cuts at: 1678 5718 1678 Size: 4040 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3032 3064 5212 5244 5286 Size: 32 135 32 1460 32 2148 32 42 Cuts at: 5286 5318 1373 Size: 32 2626 Fragments arranged by size: 2626 2148 1460 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3550 3915 4161 669 Size: 988 281 1612 365 246 3079 Fragments arranged by size: 3079 1612 988 365 281 246 HindIII A'AGCT_T Cuts at: 748 3445 748 Size: 2697 3874 HpaI GTT'AAC Cuts at: 4161 4161 Size: 6571 KpnI G_GTAC'C Cuts at: 3896 3896 Size: 6571 MfeI C'AATT_G Cuts at: 4170 4170 Size: 6571 MluI A'CGCG_T Cuts at: 6447 6447 Size: 6571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2835 5685 5869 1305 Size: 1530 2850 184 2007 Fragments arranged by size: 2850 2007 1530 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3782 10 Size: 54 1425 343 1950 2799 Fragments arranged by size: 2799 1950 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 2306 3197 3228 3677 4529 4888 Size: 648 1119 21 891 31 449 852 359 Cuts at: 4888 5047 518 Size: 159 2042 Fragments arranged by size: 2042 1119 891 852 648 449 359 159 31 21 MspA1I CmG'CkG Cuts at: 1606 2537 3597 3820 3987 4664 5812 6057 1606 Size: 931 1060 223 167 677 1148 245 2120 Fragments arranged by size: 2120 1148 1060 931 677 245 223 167 NcoI C'CATG_G Cuts at: 513 1065 2638 513 Size: 552 1573 4446 Fragments arranged by size: 4446 1573 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6571 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6571 NotI GC'GGCC_GC Cuts at: 4018 4018 Size: 6571 NsiI A_TGCA'T Cuts at: 2223 2223 Size: 6571 NspI r_CATG'y Cuts at: 1132 1229 3701 3852 3929 5474 1132 Size: 97 2472 151 77 1545 2229 Fragments arranged by size: 2472 2229 1545 151 97 77 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6571 PciI A'CATG_T Cuts at: 5470 5470 Size: 6571 PflMI CCAn_nnn'nTGG Cuts at: 1108 2639 2819 1108 Size: 1531 180 4860 Fragments arranged by size: 4860 1531 180 PfoI T'CCnGG_A Cuts at: 2086 2596 2086 Size: 510 6061 PmeI GTTT'AAAC Cuts at: 3875 3875 Size: 6571 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4640 4672 6153 6185 4640 Size: 32 1481 32 5026 Fragments arranged by size: 5026 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3181 3181 Size: 6571 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6571 PsiI TTA'TAA Cuts at: 4141 4141 Size: 6571 PspOMI G'GGCC_C Cuts at: 3328 3328 Size: 6571 PstI C_TGCA'G Cuts at: 830 3337 3923 830 Size: 2507 586 3478 Fragments arranged by size: 3478 2507 586 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6571 PvuII CAG'CTG Cuts at: 2537 3820 2537 Size: 1283 5288 SalI G'TCGA_C Cuts at: 1936 3913 1936 Size: 1977 4594 SanDI GG'GwC_CC Cuts at: 3181 3181 Size: 6571 SbfI CC_TGCA'GG Cuts at: 3337 3923 3337 Size: 586 5985 ScaI AGT'ACT Cuts at: 4819 6531 4819 Size: 1712 4859 SfcI C'TryA_G Cuts at: 826 1045 2785 3333 3919 5054 5735 5926 826 Size: 219 1740 548 586 1135 681 191 1471 Fragments arranged by size: 1740 1471 1135 681 586 548 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6571 SmaI CCC'GGG Cuts at: 1872 3616 3898 1872 Size: 1744 282 4545 Fragments arranged by size: 4545 1744 282 SmlI C'TyrA_G Cuts at: 820 1017 1943 4675 5576 5838 820 Size: 197 926 2732 901 262 1553 Fragments arranged by size: 2732 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4362 493 Size: 3869 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6571 SphI G_CATG'C Cuts at: 1132 3929 1132 Size: 2797 3774 SspI AAT'ATT Cuts at: 5 52 3301 4495 5 Size: 47 3249 1194 2081 Fragments arranged by size: 3249 2081 1194 47 StuI AGG'CCT Cuts at: 1517 2152 1517 Size: 635 5936 StyI C'CwwG_G Cuts at: 513 1065 2638 2889 3522 3645 4010 513 Size: 552 1573 251 633 123 365 3074 Fragments arranged by size: 3074 1573 633 552 365 251 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3018 3812 4598 4615 4768 4953 5398 Size: 1255 1438 794 786 17 153 185 445 Cuts at: 5398 325 Size: 1498 Fragments arranged by size: 1498 1438 1255 794 786 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 4817 6529 Size: 274 80 33 51 1443 300 2540 1712 Cuts at: 6529 96 Size: 138 Fragments arranged by size: 2540 1712 1443 300 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3173 3205 6531 6563 3173 Size: 32 3326 32 3181 Fragments arranged by size: 3326 3181 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6571 XbaI T'CTAG_A Cuts at: 3404 3907 3404 Size: 503 6068 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2326 2326 Size: 6571 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6571 XmaI C'CCGG_G Cuts at: 1870 3614 3896 1870 Size: 1744 282 4545 Fragments arranged by size: 4545 1744 282 XmnI GAAnn'nnTTC Cuts at: 4700 4700 Size: 6571 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4379 276 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI