Restriction map for
pFN21AB8368
(Linear) (Six-base) MAPSORT of: KIBB8368.seq Check: 308 from: 1 to: 1866
KIBB8368.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 64 142 1321 1866
Size: 64 78 1179 545
Fragments arranged by size:
1179 545 78 64
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1866
Size: 887 979
AgeI A'CCGG_T
Cuts at: 0 238 1866
Size: 238 1628
AlwNI CAG_nnn'CTG
Cuts at: 0 814 1866
Size: 814 1052
ApaI G_GGCC'C
Cuts at: 0 1327 1866
Size: 1327 539
ApaLI G'TGCA_C
Cuts at: 0 1334 1866
Size: 1334 532
ApoI r'AATT_y
Cuts at: 0 506 1866
Size: 506 1360
AvaI C'yCGr_G
Cuts at: 0 520 1609 1866
Size: 520 1089 257
Fragments arranged by size:
1089 520 257
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 630 663 692 725 1866
Size: 630 33 29 33 1141
Fragments arranged by size:
1141 630 33 33 29
BamHI G'GATC_C
Cuts at: 0 1029 1228 1836 1866
Size: 1029 199 608 30
Fragments arranged by size:
1029 608 199 30
BanI G'GyrC_C
Cuts at: 0 1680 1866
Size: 1680 186
BanII G_rGCy'C
Cuts at: 0 1327 1866
Size: 1327 539
BbsI GAAGACnn'nnnn_
Cuts at: 0 428 1866
Size: 428 1438
BciVI GTATCCnnnnn_n'
Cuts at: 0 631 1458 1866
Size: 631 827 408
Fragments arranged by size:
827 631 408
BfrBI ATG'CAT
Cuts at: 0 216 1866
Size: 216 1650
BglI GCCn_nnn'nGGC
Cuts at: 0 263 1732 1866
Size: 263 1469 134
Fragments arranged by size:
1469 263 134
BglII A'GATC_T
Cuts at: 0 95 1866
Size: 95 1771
Bme1580I G_kGCm'C
Cuts at: 0 1327 1338 1866
Size: 1327 11 528
Fragments arranged by size:
1327 528 11
BmgBI CAC'GTC
Cuts at: 0 360 1866
Size: 360 1506
BmrI ACTGGGnnnn_n'
Cuts at: 0 1176 1866
Size: 1176 690
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 226 1866
Size: 226 1640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 663 1795 1866
Size: 663 1132 71
Fragments arranged by size:
1132 663 71
BsaI GGTCTCn'nnnn_
Cuts at: 0 458 1866
Size: 458 1408
BsaBI GATnn'nnATC
Cuts at: 0 842 1866
Size: 842 1024
BsaWI w'CCGG_w
Cuts at: 0 238 1866
Size: 238 1628
BseYI C'CCAG_C
Cuts at: 0 553 1261 1421 1815 1866
Size: 553 708 160 394 51
Fragments arranged by size:
708 553 394 160 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1824 1866
Size: 1824 42
BsiHKAI G_wGCw'C
Cuts at: 0 1338 1866
Size: 1338 528
Bsp1286I G_dGCh'C
Cuts at: 0 1327 1338 1866
Size: 1327 11 528
Fragments arranged by size:
1327 528 11
BspHI T'CATG_A
Cuts at: 0 299 1866
Size: 299 1567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 64 142 802 1321 1351 1866
Size: 64 78 660 519 30 515
Fragments arranged by size:
660 519 515 78 64 30
BsrDI GCAATG_nn'
Cuts at: 0 1564 1866
Size: 1564 302
BsrFI r'CCGG_y
Cuts at: 0 238 1338 1866
Size: 238 1100 528
Fragments arranged by size:
1100 528 238
BstEII G'GTnAC_C
Cuts at: 0 789 1866
Size: 789 1077
BstXI CCAn_nnnn'nTGG
Cuts at: 0 553 1026 1819 1866
Size: 553 473 793 47
Fragments arranged by size:
793 553 473 47
BstYI r'GATC_y
Cuts at: 0 95 542 713 1029 1228 1279 1686 1836
Size: 95 447 171 316 199 51 407 150
Cuts at: 1836 1866
Size: 30
Fragments arranged by size:
447 407 316 199 171 150 95 51
30
Bsu36I CC'TnA_GG
Cuts at: 0 1130 1866
Size: 1130 736
BtgI C'CryG_G
Cuts at: 0 633 1866
Size: 633 1233
BtsI GCAGTG_nn'
Cuts at: 0 770 1784 1866
Size: 770 1014 82
Fragments arranged by size:
1014 770 82
DraI TTT'AAA
Cuts at: 0 102 1376 1866
Size: 102 1274 490
Fragments arranged by size:
1274 490 102
EaeI y'GGCC_r
Cuts at: 0 1340 1775 1866
Size: 1340 435 91
Fragments arranged by size:
1340 435 91
EarI CTCTTCn'nnn_
Cuts at: 0 1400 1866
Size: 1400 466
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 794 1866
Size: 794 1072
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 663 887 1795 1866
Size: 663 224 908 71
Fragments arranged by size:
908 663 224 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 610 1866
Size: 610 1256
EcoO109I rG'GnC_Cy
Cuts at: 0 1176 1324 1734 1866
Size: 1176 148 410 132
Fragments arranged by size:
1176 410 148 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1027 1059 1866
Size: 1027 32 807
Fragments arranged by size:
1027 807 32
HincII GTy'rAC
Cuts at: 0 1545 1866
Size: 1545 321
HindIII A'AGCT_T
Cuts at: 0 1440 1866
Size: 1440 426
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 1866
Size: 830 1036
MscI TGG'CCA
Cuts at: 0 1777 1866
Size: 1777 89
MslI CAynn'nnrTG
Cuts at: 0 280 301 1192 1223 1672 1866
Size: 280 21 891 31 449 194
Fragments arranged by size:
891 449 280 194 31 21
MspA1I CmG'CkG
Cuts at: 0 532 1592 1815 1866
Size: 532 1060 223 51
Fragments arranged by size:
1060 532 223 51
NcoI C'CATG_G
Cuts at: 0 633 1866
Size: 633 1233
NsiI A_TGCA'T
Cuts at: 0 218 1866
Size: 218 1648
NspI r_CATG'y
Cuts at: 0 1696 1847 1866
Size: 1696 151 19
Fragments arranged by size:
1696 151 19
PflMI CCAn_nnn'nTGG
Cuts at: 0 634 814 1866
Size: 634 180 1052
Fragments arranged by size:
1052 634 180
PfoI T'CCnGG_A
Cuts at: 0 81 591 1866
Size: 81 510 1275
Fragments arranged by size:
1275 510 81
PpuMI rG'GwC_Cy
Cuts at: 0 1176 1866
Size: 1176 690
PspOMI G'GGCC_C
Cuts at: 0 1323 1866
Size: 1323 543
PstI C_TGCA'G
Cuts at: 0 1332 1866
Size: 1332 534
PvuII CAG'CTG
Cuts at: 0 532 1815 1866
Size: 532 1283 51
Fragments arranged by size:
1283 532 51
SanDI GG'GwC_CC
Cuts at: 0 1176 1866
Size: 1176 690
SbfI CC_TGCA'GG
Cuts at: 0 1332 1866
Size: 1332 534
SfcI C'TryA_G
Cuts at: 0 780 1328 1866
Size: 780 548 538
Fragments arranged by size:
780 548 538
SmaI CCC'GGG
Cuts at: 0 1611 1866
Size: 1611 255
SspI AAT'ATT
Cuts at: 0 1296 1866
Size: 1296 570
StuI AGG'CCT
Cuts at: 0 147 1866
Size: 147 1719
StyI C'CwwG_G
Cuts at: 0 633 884 1517 1640 1866
Size: 633 251 633 123 226
Fragments arranged by size:
633 633 251 226 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1013 1807 1866
Size: 1013 794 59
Fragments arranged by size:
1013 794 59
TatI w'GTAC_w
Cuts at: 0 272 1866
Size: 272 1594
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1168 1200 1866
Size: 1168 32 666
Fragments arranged by size:
1168 666 32
XbaI T'CTAG_A
Cuts at: 0 1399 1866
Size: 1399 467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 321 1866
Size: 321 1545
XmaI C'CCGG_G
Cuts at: 0 1609 1866
Size: 1609 257
Enzymes that do cut:
AarI AcuI AgeI AlwNI ApaI ApaLI ApoI AvaI
BaeI BamHI BanI BanII BbsI BciVI BfrBI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI BsaI BsaBI
BsaWI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI
EaeI EarI EciI Eco57MI EcoNI EcoO109I Hin4I HincII
HindIII MmeI MscI MslI MspA1I NcoI NsiI NspI
PflMI PfoI PpuMI PspOMI PstI PvuII SanDI SbfI
SfcI SmaI SspI StuI StyI TaqII TatI TstI
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AloI AscI AseI AsiSI AvrII BbeI
BbvCI BcgI BclI BlpI BmtI Bpu10I BpuEI BsaAI
BsaHI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI
ClaI CspCI DraIII DrdI EagI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SwaI Tth111I XhoI XmnI
ZraI