Restriction map for
pFN21AB8362
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# NO CUT ENZYMES ( 111 enzymes) #
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AclI AcsI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AviII AvrII BalI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsiWI Bsp1407I
BspDI BspHI BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HindIII HpaI
KasI KspI MamI MfeI MluI
MluNI MscI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SapI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tsp509I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 221 GGTACC G^GTACC
AccIII 691 TCCGGA T^CCGGA
AflIII 1196 ACATGT A^C[GA][CT]GT
ApaI 382 GGGCCC GGGCC^C
Asp718I 221 GGTACC G^GTACC
AspHI 52 GTGCTC G[AT]GC[AT]^C
AspI 1003 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 274 GGATCC G^GATCC
BbvCI 90 CCTCAGC CCTCAGC(-5/-2)
BfaI 1106 CTAG C^TAG
BsaAI 673 TACGTG [CT]AC^GT[GA]
BsaWI 691 TCCGGA [AT]^CCGG[AT]
BseAI 691 TCCGGA T^CCGGA
BsgI 214 GTGCAG GTGCAG(16/14)
BsiHKAI 52 GTGCTC G[AT]GC[AT]^C
BsmAI 481 GTCTC GTCTC(1/5)
BsmBI 480 CGTCTC CGTCTC(1/5)
BsmI 990 GAATGC GAATGC(1/-1)
BspEI 691 TCCGGA T^CCGGA
BspLU11I 1196 ACATGT A^CATGT
BsrDI 1013 GCAATG GCAATG(2/0)
BsrI 1143 ACTGG ACTGG(1/-1)
BstAPI 851 GCAACGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1314 GGTCACC G^GT[ACGT]ACC
BstPI 1314 GGTCACC G^GT[ACGT]ACC
BstXI 597 CCACTCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 274 GGATCC [GA]^GATC[CT]
DpnII 275 GATC ^GATC
EcoO65I 1314 GGTCACC G^GT[ACGT]ACC
HgaI 109 GACGC GACGC(5/10)
HincII 639 GTCGAC GT[CT]^[GA]AC
HindII 639 GTCGAC GT[CT]^[GA]AC
KpnI 221 GGTACC GGTAC^C
MaeI 1106 CTAG C^TAG
MboI 275 GATC ^GATC
MflI 274 GGATCC [GA]^GATC[CT]
MroI 691 TCCGGA T^CCGGA
MspA1I 124 CCGCTG C[AC]G^C[GT]G
NaeI 517 GCCGGC GCC^GGC
NcoI 1322 CCATGG C^CATGG
NdeII 275 GATC ^GATC
NgoMIV 517 GCCGGC G^CCGGC
PflFI 1003 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 974 CCACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1001 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1193 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 923 CTGCAG CTGCA^G
SalI 639 GTCGAC G^TCGAC
Sau3AI 275 GATC ^GATC
SexAI 557 ACCAGGT A^CC[AT]GGT
SfcI 923 CTGCAG C^T[GA][CT]AG
SfiI 516 GGCCGGCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 550 CTCAAG C^T[CT][GA]AG
SspI 1045 AATATT AAT^ATT
TfiI 336 GAATC G^A[AT]TC
Tth111I 1003 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 974 CCACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 707 CCAGCAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 274 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 394 GTCTAC GT^[AC][GT]AC
639 GTCGAC
AccII 23 CGCG CG^CG
111 CGCG
AlwI 275 GATCC GGATC(4/5)
274 GGATC
BanI 221 GGTACC G^G[CT][GA]CC
561 GGTGCC
BglI 517 GCCGGCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
713 GCCTGCTGGGC
BsiEI 515 CGGCCG CG[GA][CT]^CG
1178 CGGCCG
BstUI 23 CGCG CG^CG
111 CGCG
CfoI 24 GCGC GCG^C
115 GCGC
EaeI 515 CGGCCG [CT]^GGCC[GA]
1178 CGGCCG
EagI 515 CGGCCG C^GGCCG
1178 CGGCCG
EarI 448 CTCTTC CTCTTC(1/4)
697 CTCTTC
EclXI 515 CGGCCG C^GGCCG
1178 CGGCCG
Eco52I 515 CGGCCG C^GGCCG
1178 CGGCCG
EcoT14I 758 CCAAGG C^C[AT][AT]GG
1322 CCATGG
HhaI 24 GCGC GCG^C
115 GCGC
HinP1I 24 GCGC G^CGC
115 GCGC
Ksp632I 448 CTCTTC CTCTTC(1/4)
697 CTCTTC
MaeIII 7 GTGAC ^GT[ACGT]AC
1315 GTCAC
MvnI 23 CGCG CG^CG
111 CGCG
NspI 1196 ACATGT [GA]CATG^[CT]
1241 ACATGC
SmaI 84 CCCGGG CCC^GGG
378 CCCGGG
StyI 758 CCAAGG C^C[AT][AT]GG
1322 CCATGG
Tsp45I 7 GTGAC ^GT[GC]AC
1315 GTCAC
TspRI 815 CACTG CA[GC]TG[ACGT][ACGT]^
1142 CACTG
XmaCI 84 CCCGGG C^CCGGG
378 CCCGGG
XmaI 84 CCCGGG C^CCGGG
378 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AatII AciI AcyI AfaI AluI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BssKI BstF5I BstNI Cac8I
Cfr10I Cfr13I DdeI DraII DsaI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII Hin1I HinfI HpaII
HphI ItaI MaeII MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NlaIII NlaIV PleI RsaI
Sau96I ScrFI SfaNI TaqI Tru9I
TseI TthHB8I