Restriction map for
pFN21AB8362
(Linear) (Six-base) MAPSORT of: KIBB8362.seq Check: 3084 from: 1 to: 1329
KIBB8362.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AatII G_ACGT'C
Cuts at: 0 361 482 946 1329
Size: 361 121 464 383
Fragments arranged by size:
464 383 361 121
AccI GT'mk_AC
Cuts at: 0 395 640 1329
Size: 395 245 689
Fragments arranged by size:
689 395 245
Acc65I G'GTAC_C
Cuts at: 0 221 1329
Size: 221 1108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1209 1329
Size: 1209 120
AflIII A'CryG_T
Cuts at: 0 1196 1329
Size: 1196 133
AleI CACnn'nnGTG
Cuts at: 0 602 1329
Size: 602 727
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1147 1329
Size: 1147 182
ApaI G_GGCC'C
Cuts at: 0 386 1329
Size: 386 943
AvaI C'yCGr_G
Cuts at: 0 84 378 679 1329
Size: 84 294 301 650
Fragments arranged by size:
650 301 294 84
BamHI G'GATC_C
Cuts at: 0 274 1329
Size: 274 1055
BanI G'GyrC_C
Cuts at: 0 221 561 1329
Size: 221 340 768
Fragments arranged by size:
768 340 221
BanII G_rGCy'C
Cuts at: 0 386 866 1028 1329
Size: 386 480 162 301
Fragments arranged by size:
480 386 301 162
BbvCI CC'TCA_GC
Cuts at: 0 91 1329
Size: 91 1238
BglI GCCn_nnn'nGGC
Cuts at: 0 523 719 1329
Size: 523 196 610
Fragments arranged by size:
610 523 196
Bme1580I G_kGCm'C
Cuts at: 0 386 566 680 1329
Size: 386 180 114 649
Fragments arranged by size:
649 386 180 114
BmgBI CAC'GTC
Cuts at: 0 177 1329
Size: 177 1152
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 242 705 1329
Size: 242 463 624
Fragments arranged by size:
624 463 242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1002 1165 1173 1218 1290 1329
Size: 607 395 163 8 45 72 39
Fragments arranged by size:
607 395 163 72 45 39 8
Bpu10I CC'TnA_GC
Cuts at: 0 91 1207 1329
Size: 91 1116 122
Fragments arranged by size:
1116 122 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 1329
Size: 535 794
BsaAI yAC'GTr
Cuts at: 0 675 1329
Size: 675 654
BsaHI Gr'CG_yC
Cuts at: 0 358 479 943 1329
Size: 358 121 464 386
Fragments arranged by size:
464 386 358 121
BsaWI w'CCGG_w
Cuts at: 0 691 1329
Size: 691 638
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 358 388 1186 1216 1288 1318
Size: 310 30 18 30 798 30 72 30
Cuts at: 1318 1329
Size: 11
Fragments arranged by size:
798 310 72 30 30 30 30 18
11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 405 624 999 1329
Size: 148 257 219 375 330
Fragments arranged by size:
375 330 257 219 148
BseYI C'CCAG_C
Cuts at: 0 26 706 717 1329
Size: 26 680 11 612
Fragments arranged by size:
680 612 26 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1329
Size: 235 1094
BsiEI CG_ry'CG
Cuts at: 0 518 1181 1329
Size: 518 663 148
Fragments arranged by size:
663 518 148
BsiHKAI G_wGCw'C
Cuts at: 0 56 1329
Size: 56 1273
BsmI GAATG_Cn'
Cuts at: 0 996 1329
Size: 996 333
BsmBI CGTCTCn'nnnn_
Cuts at: 0 486 1329
Size: 486 843
Bsp1286I G_dGCh'C
Cuts at: 0 56 386 566 680 866 1028 1329
Size: 56 330 180 114 186 162 301
Fragments arranged by size:
330 301 186 180 162 114 56
BspEI T'CCGG_A
Cuts at: 0 691 1329
Size: 691 638
BsrDI GCAATG_nn'
Cuts at: 0 1020 1329
Size: 1020 309
BsrFI r'CCGG_y
Cuts at: 0 517 733 890 1329
Size: 517 216 157 439
Fragments arranged by size:
517 439 216 157
BstAPI GCAn_nnn'nTGC
Cuts at: 0 857 1329
Size: 857 472
BstEII G'GTnAC_C
Cuts at: 0 1314 1329
Size: 1314 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 604 1329
Size: 604 725
BstYI r'GATC_y
Cuts at: 0 274 1329
Size: 274 1055
BtgI C'CryG_G
Cuts at: 0 209 237 369 603 1322 1329
Size: 209 28 132 234 719 7
Fragments arranged by size:
719 234 209 132 28 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 479 1329
Size: 479 850
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1002 1037 1329
Size: 1002 35 292
Fragments arranged by size:
1002 292 35
EaeI y'GGCC_r
Cuts at: 0 515 1178 1329
Size: 515 663 151
Fragments arranged by size:
663 515 151
EagI C'GGCC_G
Cuts at: 0 515 1178 1329
Size: 515 663 151
Fragments arranged by size:
663 515 151
EarI CTCTTCn'nnn_
Cuts at: 0 454 703 1329
Size: 454 249 626
Fragments arranged by size:
626 454 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 1329
Size: 28 1301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1002 1165 1173 1209 1218 1290 1329
Size: 607 395 163 8 36 9 72 39
Fragments arranged by size:
607 395 163 72 39 36 9 8
EcoO109I rG'GnC_Cy
Cuts at: 0 382 383 1002 1329
Size: 382 1 619 327
Fragments arranged by size:
619 382 327 1
HincII GTy'rAC
Cuts at: 0 641 1329
Size: 641 688
KpnI G_GTAC'C
Cuts at: 0 225 1329
Size: 225 1104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1329
Size: 253 1076
MslI CAynn'nnrTG
Cuts at: 0 602 1261 1321 1329
Size: 602 659 60 8
Fragments arranged by size:
659 602 60 8
MspA1I CmG'CkG
Cuts at: 0 126 1329
Size: 126 1203
NaeI GCC'GGC
Cuts at: 0 519 1329
Size: 519 810
NcoI C'CATG_G
Cuts at: 0 1322 1329
Size: 1322 7
NgoMIV G'CCGG_C
Cuts at: 0 517 1329
Size: 517 812
NspI r_CATG'y
Cuts at: 0 1200 1245 1329
Size: 1200 45 84
Fragments arranged by size:
1200 84 45
PasI CC'CwG_GG
Cuts at: 0 306 621 1329
Size: 306 315 708
Fragments arranged by size:
708 315 306
PciI A'CATG_T
Cuts at: 0 1196 1329
Size: 1196 133
PflMI CCAn_nnn'nTGG
Cuts at: 0 980 1329
Size: 980 349
PpuMI rG'GwC_Cy
Cuts at: 0 1002 1329
Size: 1002 327
PshAI GACnn'nnGTC
Cuts at: 0 1197 1329
Size: 1197 132
PspOMI G'GGCC_C
Cuts at: 0 382 1329
Size: 382 947
PstI C_TGCA'G
Cuts at: 0 927 1329
Size: 927 402
SalI G'TCGA_C
Cuts at: 0 639 1329
Size: 639 690
SanDI GG'GwC_CC
Cuts at: 0 1002 1329
Size: 1002 327
SexAI A'CCwGG_T
Cuts at: 0 557 1329
Size: 557 772
SfcI C'TryA_G
Cuts at: 0 923 1329
Size: 923 406
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 523 1329
Size: 523 806
SmaI CCC'GGG
Cuts at: 0 86 380 1329
Size: 86 294 949
Fragments arranged by size:
949 294 86
SmlI C'TyrA_G
Cuts at: 0 550 1329
Size: 550 779
SspI AAT'ATT
Cuts at: 0 1047 1329
Size: 1047 282
StyI C'CwwG_G
Cuts at: 0 758 1322 1329
Size: 758 564 7
Fragments arranged by size:
758 564 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 18 189 613 1329
Size: 18 171 424 716
Fragments arranged by size:
716 424 171 18
TatI w'GTAC_w
Cuts at: 0 683 1329
Size: 683 646
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 310 342 358 390 933 965 1186 1218
Size: 310 32 16 32 543 32 221 32
Cuts at: 1218 1258 1288 1290 1320 1329
Size: 40 30 2 30 9
Fragments arranged by size:
543 310 221 40 32 32 32 32
30 30 16 9 2
Tth111I GACn'n_nGTC
Cuts at: 0 1006 1329
Size: 1006 323
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 714 1329
Size: 714 615
XmaI C'CCGG_G
Cuts at: 0 84 378 1329
Size: 84 294 951
Fragments arranged by size:
951 294 84
ZraI GAC'GTC
Cuts at: 0 359 480 944 1329
Size: 359 121 464 385
Fragments arranged by size:
464 385 359 121
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AleI AlfI ApaI
AvaI BamHI BanI BanII BbvCI BglI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BsrDI BsrFI BstAPI BstEII BstXI BstYI BtgI
BtgZI CspCI EaeI EagI EarI EciI Eco57MI EcoO109I
HincII KpnI MmeI MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PciI PflMI PpuMI PshAI PspOMI PstI
SalI SanDI SexAI SfcI SfiI SmaI SmlI SspI
StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AloI AlwNI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI BmrI
BmtI BsaI BsaBI BsiWI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstBI BstZ17I Bsu36I BtsI ClaI DraI
DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI MfeI
MluI MscI NarI NdeI NheI NotI NruI NsiI
PacI PfoI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SapI SbfI ScaI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI
SwaI XbaI XhoI XmnI