Restriction map for
pFN21AB8353
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AclI AcsI AcyI AflII
AflIII AgeI AhdI AlwI AlwNI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BcgI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsiWI BsmI BspDI
BspHI BspLU11I BsrBI BsrFI BsrI
BssHII BstAPI BstBI BstYI CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
FspI Hin1I HincII HindII HpaI
KasI KspI MaeI MfeI MflI
MluI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI TfiI Tth111I Van91I XbaI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 908 GGTACC G^GTACC
AccIII 857 TCCGGA T^CCGGA
Alw44I 220 GTGCAC G^TGCAC
AocI 899 CCTCAGG CC^T[ACGT]AGG
ApaLI 220 GTGCAC G^TGCAC
Asp718I 908 GGTACC G^GTACC
AvaI 787 CTCGAG C^[CT]CG[GA]G
BalI 46 TGGCCA TGG^CCA
BbvCI 330 GCTGAGG CCTCAGC(-5/-2)
BclI 134 TGATCA T^GATCA
BcnI 876 CCGGG CC^[GC]GG
BsaBI 541 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 857 TCCGGA [AT]^CCGG[AT]
BseAI 857 TCCGGA T^CCGGA
BseRI 202 GAGGAG GAGGAG(10/8)
BsgI 316 GTGCAG GTGCAG(16/14)
BsiEI 438 CGACCG CG[GA][CT]^CG
BsmBI 993 CGTCTC CGTCTC(1/5)
BsoBI 787 CTCGAG C^[CT]CG[GA]G
Bsp1407I 593 TGTACA T^GTACA
BspEI 857 TCCGGA T^CCGGA
BsrGI 593 TGTACA T^GTACA
BssSI 225 CACGAG CACGAG(-5/-1)
Bst1107I 763 GTATAC GTA^TAC
BstEII 894 GGTGACC G^GT[ACGT]ACC
BstPI 894 GGTGACC G^GT[ACGT]ACC
BstZ17I 763 GTATAC GTA^TAC
Bsu36I 899 CCTCAGG CC^T[ACGT]AGG
EarI 151 GAAGAG CTCTTC(1/4)
Eco81I 899 CCTCAGG CC^T[ACGT]AGG
EcoNI 702 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 894 GGTGACC G^GT[ACGT]ACC
EcoT14I 801 CCTTGG C^C[AT][AT]GG
FbaI 134 TGATCA T^GATCA
HaeII 230 GGCGCT [GA]GCGC^[CT]
HgaI 992 GCGTC GACGC(5/10)
HindIII 157 AAGCTT A^AGCTT
KpnI 908 GGTACC GGTAC^C
Ksp632I 151 GAAGAG CTCTTC(1/4)
MamI 541 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 46 TGGCCA TGG^CCA
MroI 857 TCCGGA T^CCGGA
MscI 46 TGGCCA TGG^CCA
NciI 876 CCGGG CC^[GC]GG
PaeR7I 787 CTCGAG C^TCGAG
PpuMI 206 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1031 CTGCAG CTGCA^G
PvuII 16 CAGCTG CAG^CTG
SacI 244 GAGCTC GAGCT^C
SfcI 1031 CTGCAG C^T[GA][CT]AG
SphI 173 GCATGC GCATG^C
SspBI 593 TGTACA T^GTACA
StuI 194 AGGCCT AGG^CCT
StyI 801 CCTTGG C^C[AT][AT]GG
TaqI 788 TCGA T^CGA
Tsp509I 139 AATT ^AATT
TthHB8I 788 TCGA T^CGA
XhoI 787 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 607 GTCTAC GT^[AC][GT]AC
763 GTATAC
AccII 65 CGCG CG^CG
188 CGCG
BanII 244 GAGCTC G[GA]GC[CT]^C
967 GGGCTC
BbvI 99 GCTGC GCAGC(8/12)
1020 GCTGC
BglI 643 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
821 GCCCAGCGGGC
BsmFI 632 GGGAC GGGAC(10/14)
794 GGGAC
BspMI 209 ACCTGC ACCTGC(4/8)
746 ACCTGC
BsrDI 933 CATTGC GCAATG(2/0)
554 GCAATG
BstUI 65 CGCG CG^CG
188 CGCG
BstXI 401 CCATCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
884 CCATCCTTATGG
DpnII 135 GATC ^GATC
535 GATC
DraII 206 AGGACCT [GA]G^G[ACGT]CC[CT]
334 AGGCCCC
DsaI 441 CCGTGG C^C[GA][CT]GG
867 CCGTGG
EaeI 46 TGGCCA [CT]^GGCC[GA]
870 TGGCCG
EcoO109I 206 AGGACCT [GA]G^G[ACGT]CC[CT]
334 AGGCCCC
HinfI 940 GACTC G^A[ACGT]TC
982 GAGTC
MaeII 407 ACGT A^CGT
755 ACGT
MaeIII 895 GTGAC ^GT[ACGT]AC
984 GTCAC
MboI 135 GATC ^GATC
535 GATC
MboII 151 GAAGA GAAGA(8/7)
296 TCTTC
MseI 239 TTAA T^TAA
950 TTAA
MvnI 65 CGCG CG^CG
188 CGCG
NdeII 135 GATC ^GATC
535 GATC
NspI 27 GCATGT [GA]CATG^[CT]
173 GCATGC
PleI 940 GACTC GAGTC(4/5)
982 GAGTC
Sau3AI 135 GATC ^GATC
535 GATC
SmlI 247 CTCAAG C^T[CT][GA]AG
787 CTCGAG
Tru9I 239 TTAA T^TAA
950 TTAA
TseI 99 GCTGC G^C[AT]GC
1020 GCTGC
Tsp45I 895 GTGAC ^GT[GC]AC
984 GTCAC
TspRI 427 CACTG CA[GC]TG[ACGT][ACGT]^
1034 CAGTG
XcmI 34 CCAGAGACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
546 CCATCAGGGCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MnlI MslI MspA1I
MspI MvaI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI SfaNI