(Circular) (Six-base) MAPSORT of: pFN21AB8353.seq Check: 5192 from: 1 to: 5746 pFN21AB8353 5746 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2760 2760 Size: 5746 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2613 2769 3089 1937 Size: 676 156 320 4594 Fragments arranged by size: 4594 676 320 156 Acc65I G'GTAC_C Cuts at: 2913 3067 2913 Size: 154 5592 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3809 5193 5461 1632 Size: 2177 1384 268 1917 Fragments arranged by size: 2177 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 4645 5622 1203 Size: 3442 977 1327 Fragments arranged by size: 3442 1327 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5746 AlwNI CAG_nnn'CTG Cuts at: 3489 5061 3489 Size: 1572 4174 ApaLI G'TGCA_C Cuts at: 2225 3803 4959 2225 Size: 1578 1156 3012 Fragments arranged by size: 3012 1578 1156 ApoI r'AATT_y Cuts at: 1493 3055 3281 3433 5542 1493 Size: 1562 226 152 2109 1697 Fragments arranged by size: 2109 1697 1562 226 152 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2792 3071 1870 Size: 73 849 279 4545 Fragments arranged by size: 4545 849 279 73 BamHI G'GATC_C Cuts at: 3076 3443 3076 Size: 367 5379 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2780 2884 2913 3067 4522 Size: 325 217 627 993 104 29 154 1455 Cuts at: 4522 618 Size: 1842 Fragments arranged by size: 1842 1455 993 627 325 217 154 104 29 BanII G_rGCy'C Cuts at: 1391 2253 2976 3065 1391 Size: 862 723 89 4072 Fragments arranged by size: 4072 862 723 89 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4775 BbvCI CC'TCA_GC Cuts at: 2336 2336 Size: 5746 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3937 3971 1903 Size: 34 50 34 1916 34 3678 Fragments arranged by size: 3678 1916 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3638 4848 3638 Size: 1210 4536 BclI T'GATC_A Cuts at: 1548 2139 1548 Size: 591 5155 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2654 2832 4357 Size: 107 122 71 631 605 982 178 1525 Cuts at: 4357 136 Size: 1525 Fragments arranged by size: 1525 1525 982 631 605 178 122 107 71 BglII A'GATC_T Cuts at: 5741 5741 Size: 5746 BlpI GC'TnA_GC Cuts at: 3163 3163 Size: 5746 Bme1580I G_kGCm'C Cuts at: 1790 2229 2690 2889 3045 3807 4963 1790 Size: 439 461 199 156 762 1156 2573 Fragments arranged by size: 2573 1156 762 461 439 199 156 BmgBI CAC'GTC Cuts at: 1318 2761 1318 Size: 1443 4303 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4435 442 Size: 416 53 83 399 24 2060 958 1753 Fragments arranged by size: 2060 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2536 2536 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2422 2506 2699 2726 4406 1239 Size: 878 305 84 193 27 1680 2579 Fragments arranged by size: 2579 1680 878 305 193 84 27 Bpu10I CC'TnA_GC Cuts at: 2336 2336 Size: 5746 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2237 3871 4736 5034 2237 Size: 1634 865 298 2949 Fragments arranged by size: 2949 1634 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4409 882 Size: 617 2910 2219 Fragments arranged by size: 2910 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3537 493 Size: 711 2333 2702 Fragments arranged by size: 2702 2333 711 BsaBI GATnn'nnATC Cuts at: 2550 5740 2550 Size: 3190 2556 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3553 3935 4570 275 Size: 53 83 186 936 2020 382 635 1451 Fragments arranged by size: 2020 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2862 4179 4577 4851 4998 5409 2862 Size: 1317 398 274 147 411 3199 Fragments arranged by size: 3199 1317 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2420 2450 2666 2696 1178 Size: 30 165 30 1017 30 216 30 4228 Fragments arranged by size: 4228 1017 216 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 1186 Size: 1036 4710 BseYI C'CCAG_C Cuts at: 2019 2400 2827 2993 4949 5445 2019 Size: 381 427 166 1956 496 2320 Fragments arranged by size: 2320 1956 496 427 381 166 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2342 1163 Size: 573 606 4567 Fragments arranged by size: 4567 606 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2446 3196 3957 4985 1540 Size: 275 186 445 750 761 1028 2301 Fragments arranged by size: 2301 1028 761 750 445 275 186 BsiHKAI G_wGCw'C Cuts at: 2229 2253 2751 3807 3892 4963 2229 Size: 24 498 1056 85 1071 3012 Fragments arranged by size: 3012 1071 1056 498 85 24 BsmI GAATG_Cn' Cuts at: 3257 3350 3257 Size: 93 5653 BsmBI CGTCTCn'nnnn_ Cuts at: 3004 3004 Size: 5746 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2253 2690 2751 2889 2976 3045 Size: 399 439 24 437 61 138 87 69 Cuts at: 3045 3065 3807 3892 4963 1391 Size: 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 437 399 138 87 85 69 61 24 20 BspEI T'CCGG_A Cuts at: 2862 5409 2862 Size: 2547 3199 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2223 2760 3101 844 Size: 1379 537 341 3489 Fragments arranged by size: 3489 1379 537 341 BsrBI CCG'CTC Cuts at: 1394 1823 3633 1394 Size: 429 1810 3507 Fragments arranged by size: 3507 1810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2566 2937 4241 4415 65 Size: 1183 1318 371 1304 174 1396 Fragments arranged by size: 1396 1318 1304 1183 371 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4390 4577 1700 Size: 110 2580 187 2869 Fragments arranged by size: 2869 2580 187 110 BsrGI T'GTAC_A Cuts at: 96 2598 96 Size: 2502 3244 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 2230 3806 4818 2230 Size: 1576 1012 3158 Fragments arranged by size: 3158 1576 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5746 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 2899 1184 Size: 1715 4031 BstXI CCAn_nnnn'nTGG Cuts at: 2413 2896 2413 Size: 483 5263 BstYI r'GATC_y Cuts at: 1972 3076 3443 3828 3845 5297 5383 5395 5741 Size: 1104 367 385 17 1452 86 12 346 Cuts at: 5741 1972 Size: 1977 Fragments arranged by size: 1977 1452 1104 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2770 2770 Size: 5746 Bsu36I CC'TnA_GG Cuts at: 2905 2905 Size: 5746 BtgI C'CryG_G Cuts at: 513 1065 2446 2872 513 Size: 552 1381 426 3387 Fragments arranged by size: 3387 1381 552 426 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5007 Fragments arranged by size: 5007 683 56 BtsI GCAGTG_nn' Cuts at: 2431 3045 3262 4056 4076 2431 Size: 614 217 794 20 4101 Fragments arranged by size: 4101 794 614 217 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4753 809 Size: 507 3437 1802 Fragments arranged by size: 3437 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2051 2875 3193 Size: 54 1425 50 275 18 221 824 318 Cuts at: 3193 4082 5711 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 824 318 275 221 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2151 3676 1353 Size: 798 1525 3423 Fragments arranged by size: 3423 1525 798 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2680 4333 4707 4853 387 Size: 1064 138 1091 1653 374 146 1280 Fragments arranged by size: 1653 1280 1091 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2251 2251 Size: 5746 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2422 2506 2699 2726 3809 4406 Size: 393 485 305 84 193 27 1083 597 Cuts at: 4406 5193 5461 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1083 787 597 485 393 305 268 193 84 27 EcoNI CCTnn'n_nnAGG Cuts at: 2711 2711 Size: 5746 EcoO109I rG'GnC_Cy Cuts at: 2212 2340 2212 Size: 128 5618 EcoRI G'AATT_C Cuts at: 3055 3433 3055 Size: 378 5368 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2180 2278 2180 Size: 98 5648 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 2239 4893 1678 Size: 561 2654 2531 Fragments arranged by size: 2654 2531 561 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2224 2256 4387 4419 4461 Size: 32 135 32 652 32 2131 32 42 Cuts at: 4461 4493 1373 Size: 32 2626 Fragments arranged by size: 2626 2131 652 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3090 3336 669 Size: 988 281 1152 246 3079 Fragments arranged by size: 3079 1152 988 281 246 HindIII A'AGCT_T Cuts at: 748 2162 748 Size: 1414 4332 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 2917 3071 2917 Size: 154 5592 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4860 5044 1305 Size: 3555 184 2007 Fragments arranged by size: 3555 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2053 10 Size: 54 1425 343 221 3703 Fragments arranged by size: 3703 1425 343 221 54 MslI CAynn'nnrTG Cuts at: 518 1166 2087 2183 2411 2894 3704 4063 4222 Size: 648 921 96 228 483 810 359 159 Cuts at: 4222 518 Size: 2042 Fragments arranged by size: 2042 921 810 648 483 359 228 159 96 MspA1I CmG'CkG Cuts at: 1606 2023 2831 3025 3162 3839 4987 5232 1606 Size: 417 808 194 137 677 1148 245 2120 Fragments arranged by size: 2120 1148 808 677 417 245 194 137 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5194 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 2036 2182 3104 4649 1132 Size: 97 807 146 922 1545 2229 Fragments arranged by size: 2229 1545 922 807 146 97 PasI CC'CwG_GG Cuts at: 1348 2633 1348 Size: 1285 4461 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5746 PfoI T'CCnGG_A Cuts at: 2742 2742 Size: 5746 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2843 2875 3815 3847 5328 5360 2843 Size: 32 940 32 1481 32 3229 Fragments arranged by size: 3229 1481 940 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2212 2212 Size: 5746 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5746 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PspXI vC'TCGA_Gb Cuts at: 2792 2792 Size: 5746 PstI C_TGCA'G Cuts at: 830 3040 3098 830 Size: 2210 58 3478 Fragments arranged by size: 3478 2210 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 PvuII CAG'CTG Cuts at: 2023 2023 Size: 5746 SacI G_AGCT'C Cuts at: 2253 2253 Size: 5746 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SfcI C'TryA_G Cuts at: 826 1045 3036 3094 4229 4910 5101 826 Size: 219 1991 58 1135 681 191 1471 Fragments arranged by size: 1991 1471 1135 681 219 191 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 3073 1872 Size: 1201 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2252 2792 3850 4751 5013 820 Size: 197 926 309 540 1058 901 262 1553 Fragments arranged by size: 1553 1058 926 901 540 309 262 197 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 2182 3104 1132 Size: 1050 922 3774 Fragments arranged by size: 3774 1050 922 SspI AAT'ATT Cuts at: 5 52 3670 5 Size: 47 3618 2081 Fragments arranged by size: 3618 2081 47 StuI AGG'CCT Cuts at: 1517 2201 1517 Size: 684 5062 StyI C'CwwG_G Cuts at: 513 1065 2806 3185 513 Size: 552 1741 379 3074 Fragments arranged by size: 3074 1741 552 379 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3007 3773 3790 3943 4128 4573 325 Size: 1255 1427 766 17 153 185 445 1498 Fragments arranged by size: 1498 1427 1255 766 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2061 2598 3992 Size: 274 80 33 51 1443 84 537 1394 Cuts at: 3992 5704 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1394 537 274 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2420 2452 2666 2698 2857 2889 5706 5738 2420 Size: 32 214 32 159 32 2817 32 2428 Fragments arranged by size: 2817 2428 214 159 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5746 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2046 2558 2046 Size: 512 5234 XhoI C'TCGA_G Cuts at: 1943 2792 1943 Size: 849 4897 XmaI C'CCGG_G Cuts at: 1870 3071 1870 Size: 1201 4545 XmnI GAAnn'nnTTC Cuts at: 3875 3875 Size: 5746 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI