Restriction map for
pFN21AB8352
(Linear) (Six-base) MAPSORT of: KIBB8352.seq Check: 6215 from: 1 to: 2079
KIBB8352.orf
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 353 2079
Size: 353 1726
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1284 2079
Size: 820 464 795
Fragments arranged by size:
820 795 464
AfeI AGC'GCT
Cuts at: 0 259 2079
Size: 259 1820
AflIII A'CryG_T
Cuts at: 0 1583 2079
Size: 1583 496
AleI CACnn'nnGTG
Cuts at: 0 1864 2079
Size: 1864 215
AlwNI CAG_nnn'CTG
Cuts at: 0 544 1023 1632 1959 2001 2079
Size: 544 479 609 327 42 78
Fragments arranged by size:
609 544 479 327 78 42
ApaI G_GGCC'C
Cuts at: 0 19 1983 2054 2079
Size: 19 1964 71 25
Fragments arranged by size:
1964 71 25 19
ApaLI G'TGCA_C
Cuts at: 0 328 2079
Size: 328 1751
ApoI r'AATT_y
Cuts at: 0 2068 2079
Size: 2068 11
AvaI C'yCGr_G
Cuts at: 0 18 423 695 1046 1252 2079
Size: 18 405 272 351 206 827
Fragments arranged by size:
827 405 351 272 206 18
AvrII C'CTAG_G
Cuts at: 0 1716 2079
Size: 1716 363
BamHI G'GATC_C
Cuts at: 0 1849 2079
Size: 1849 230
BanI G'GyrC_C
Cuts at: 0 40 63 125 183 383 412 700 1234
Size: 40 23 62 58 200 29 288 534
Cuts at: 1234 1360 2079
Size: 126 719
Fragments arranged by size:
719 534 288 200 126 62 58 40
29 23
BanII G_rGCy'C
Cuts at: 0 19 29 116 179 472 551 634 657
Size: 19 10 87 63 293 79 83 23
Cuts at: 657 725 1605 1983 2054 2079
Size: 68 880 378 71 25
Fragments arranged by size:
880 378 293 87 83 79 71 68
63 25 23 19 10
BbeI G_GCGC'C
Cuts at: 0 44 187 2079
Size: 44 143 1892
Fragments arranged by size:
1892 143 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 1357 2079
Size: 1357 722
BbvCI CC'TCA_GC
Cuts at: 0 489 1016 1403 2079
Size: 489 527 387 676
Fragments arranged by size:
676 527 489 387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1808 1842 1883 1917 2079
Size: 1808 34 41 34 162
Fragments arranged by size:
1808 162 41 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 316 1721 2079
Size: 316 1405 358
Fragments arranged by size:
1405 358 316
BglII A'GATC_T
Cuts at: 0 1493 2079
Size: 1493 586
Bme1580I G_kGCm'C
Cuts at: 0 19 332 703 1237 1659 1983 2054 2079
Size: 19 313 371 534 422 324 71 25
Fragments arranged by size:
534 422 371 324 313 71 25 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 486 581 838 985 1433 2079
Size: 130 356 95 257 147 448 646
Fragments arranged by size:
646 448 356 257 147 130 95
Bpu10I CC'TnA_GC
Cuts at: 0 489 545 1016 1403 2079
Size: 489 56 471 387 676
Fragments arranged by size:
676 489 471 387 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 2079
Size: 709 1370
BsaI GGTCTCn'nnnn_
Cuts at: 0 1318 2079
Size: 1318 761
BsaAI yAC'GTr
Cuts at: 0 1584 2079
Size: 1584 495
BsaBI GATnn'nnATC
Cuts at: 0 1924 2079
Size: 1924 155
BsaHI Gr'CG_yC
Cuts at: 0 41 104 184 190 197 2079
Size: 41 63 80 6 7 1882
Fragments arranged by size:
1882 80 63 41 7 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 850 880 2079
Size: 850 30 1199
Fragments arranged by size:
1199 850 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 733 1270 1411 1420 2079
Size: 733 537 141 9 659
Fragments arranged by size:
733 659 537 141 9
BseYI C'CCAG_C
Cuts at: 0 44 430 510 1078 1143 1192 1776 1832
Size: 44 386 80 568 65 49 584 56
Cuts at: 1832 2079
Size: 247
Fragments arranged by size:
584 568 386 247 80 65 56 49
44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1519 2079
Size: 428 1091 560
Fragments arranged by size:
1091 560 428
BsiHKAI G_wGCw'C
Cuts at: 0 332 551 634 725 915 1441 2079
Size: 332 219 83 91 190 526 638
Fragments arranged by size:
638 526 332 219 190 91 83
BsmBI CGTCTCn'nnnn_
Cuts at: 0 979 2079
Size: 979 1100
Bsp1286I G_dGCh'C
Cuts at: 0 19 29 116 179 332 472 551 634
Size: 19 10 87 63 153 140 79 83
Cuts at: 634 657 703 725 915 1237 1441 1605 1659
Size: 23 46 22 190 322 204 164 54
Cuts at: 1659 1983 2054 2079
Size: 324 71 25
Fragments arranged by size:
324 322 204 190 164 153 140 87
83 79 71 63 54 46 25 23
22 19 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 1256 1441 2079
Size: 353 903 185 638
Fragments arranged by size:
903 638 353 185
BsrFI r'CCGG_y
Cuts at: 0 1795 2079
Size: 1795 284
BssHII G'CGCG_C
Cuts at: 0 169 2079
Size: 169 1910
BssSI C'ACGA_G
Cuts at: 0 325 2079
Size: 325 1754
BstXI CCAn_nnnn'nTGG
Cuts at: 0 136 1866 2079
Size: 136 1730 213
Fragments arranged by size:
1730 213 136
BstYI r'GATC_y
Cuts at: 0 1493 1849 2079
Size: 1493 356 230
Fragments arranged by size:
1493 356 230
Bsu36I CC'TnA_GG
Cuts at: 0 1786 1805 2079
Size: 1786 19 274
Fragments arranged by size:
1786 274 19
BtgI C'CryG_G
Cuts at: 0 1089 1621 1697 1865 2079
Size: 1089 532 76 168 214
Fragments arranged by size:
1089 532 214 168 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1467 2079
Size: 1467 612
BtsI GCAGTG_nn'
Cuts at: 0 416 836 978 1441 2079
Size: 416 420 142 463 638
Fragments arranged by size:
638 463 420 416 142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 872 907 2079
Size: 872 35 1172
Fragments arranged by size:
1172 872 35
EaeI y'GGCC_r
Cuts at: 0 2 275 317 2079
Size: 2 273 42 1762
Fragments arranged by size:
1762 273 42 2
EarI CTCTTCn'nnn_
Cuts at: 0 787 1388 2079
Size: 787 601 691
Fragments arranged by size:
787 691 601
EcoICRI GAG'CTC
Cuts at: 0 549 632 723 2079
Size: 549 83 91 1356
Fragments arranged by size:
1356 549 91 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 486 581 820 838 985 1284 1433
Size: 130 356 95 239 18 147 299 149
Cuts at: 1433 2079
Size: 646
Fragments arranged by size:
646 356 299 239 149 147 130 95
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 646 850 1252 1789 1969 2035 2079
Size: 646 204 402 537 180 66 44
Fragments arranged by size:
646 537 402 204 180 66 44
EcoO109I rG'GnC_Cy
Cuts at: 0 541 1684 1979 1980 2050 2079
Size: 541 1143 295 1 70 29
Fragments arranged by size:
1143 541 295 70 29 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 587 858 2079
Size: 587 271 1221
Fragments arranged by size:
1221 587 271
HaeII r_GCGC'y
Cuts at: 0 44 187 261 1642 2079
Size: 44 143 74 1381 437
Fragments arranged by size:
1381 437 143 74 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 850 882 1013 1045 1359 1391 2079
Size: 850 32 131 32 314 32 688
Fragments arranged by size:
850 688 314 131 32 32 32
KasI G'GCGC_C
Cuts at: 0 40 183 2079
Size: 40 143 1896
Fragments arranged by size:
1896 143 40
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1133 2079
Size: 1133 946
MslI CAynn'nnrTG
Cuts at: 0 1486 1864 2079
Size: 1486 378 215
Fragments arranged by size:
1486 378 215
MspA1I CmG'CkG
Cuts at: 0 351 864 1518 1674 1998 2079
Size: 351 513 654 156 324 81
Fragments arranged by size:
654 513 351 324 156 81
NaeI GCC'GGC
Cuts at: 0 1797 2079
Size: 1797 282
NarI GG'CG_CC
Cuts at: 0 41 184 2079
Size: 41 143 1895
Fragments arranged by size:
1895 143 41
NcoI C'CATG_G
Cuts at: 0 1089 1621 2079
Size: 1089 532 458
Fragments arranged by size:
1089 532 458
NgoMIV G'CCGG_C
Cuts at: 0 1795 2079
Size: 1795 284
PasI CC'CwG_GG
Cuts at: 0 71 2079
Size: 71 2008
PflMI CCAn_nnn'nTGG
Cuts at: 0 1698 1959 2079
Size: 1698 261 120
Fragments arranged by size:
1698 261 120
PspOMI G'GGCC_C
Cuts at: 0 15 1979 2050 2079
Size: 15 1964 71 29
Fragments arranged by size:
1964 71 29 15
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 910 942 2079
Size: 910 32 1137
Fragments arranged by size:
1137 910 32
PstI C_TGCA'G
Cuts at: 0 350 650 831 1464 1679 1997 2079
Size: 350 300 181 633 215 318 82
Fragments arranged by size:
633 350 318 300 215 181 82
PvuII CAG'CTG
Cuts at: 0 351 864 1518 1674 1998 2079
Size: 351 513 654 156 324 81
Fragments arranged by size:
654 513 351 324 156 81
SacI G_AGCT'C
Cuts at: 0 551 634 725 2079
Size: 551 83 91 1354
Fragments arranged by size:
1354 551 91 83
SbfI CC_TGCA'GG
Cuts at: 0 650 2079
Size: 650 1429
SexAI A'CCwGG_T
Cuts at: 0 1385 2079
Size: 1385 694
SfcI C'TryA_G
Cuts at: 0 346 646 827 1460 1675 1993 2079
Size: 346 300 181 633 215 318 86
Fragments arranged by size:
633 346 318 300 215 181 86
SfoI GGC'GCC
Cuts at: 0 42 185 2079
Size: 42 143 1894
Fragments arranged by size:
1894 143 42
SmaI CCC'GGG
Cuts at: 0 20 697 1048 2079
Size: 20 677 351 1031
Fragments arranged by size:
1031 677 351 20
SmlI C'TyrA_G
Cuts at: 0 724 2079
Size: 724 1355
SrfI GCCC'GGGC
Cuts at: 0 1048 2079
Size: 1048 1031
StuI AGG'CCT
Cuts at: 0 463 994 1557 2079
Size: 463 531 563 522
Fragments arranged by size:
563 531 522 463
StyI C'CwwG_G
Cuts at: 0 1089 1328 1621 1716 1881 2079
Size: 1089 239 293 95 165 198
Fragments arranged by size:
1089 293 239 198 165 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 128 253 718 737 865 896 1152 1447
Size: 128 125 465 19 128 31 256 295
Cuts at: 1447 2079
Size: 632
Fragments arranged by size:
632 465 295 256 128 128 125 31
19
TatI w'GTAC_w
Cuts at: 0 1259 2079
Size: 1259 820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 960 1007 1116 1695 2079
Size: 960 47 109 579 384
Fragments arranged by size:
960 579 384 109 47
XmaI C'CCGG_G
Cuts at: 0 18 695 1046 2079
Size: 18 677 351 1033
Fragments arranged by size:
1033 677 351 18
XmnI GAAnn'nnTTC
Cuts at: 0 922 2079
Size: 922 1157
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BglI BglII Bme1580I BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssHII BssSI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI
MmeI MslI MspA1I NaeI NarI NcoI NgoMIV PasI
PflMI PspOMI PsrI PstI PvuII SacI SbfI SexAI
SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII
TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI BaeI BciVI BclI BfrBI
BlpI BmgBI BmrI BmtI BplI BsaWI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI
BstEII BstZ17I ClaI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NdeI NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PvuI RsrII SacII SalI
SanDI SapI ScaI SfiI SgfI SgrAI SnaBI SpeI
SphI SspI SwaI TstI Tth111I XbaI XhoI ZraI