################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvaI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlpI Bpu1102I BpuAI BsaAI BsaBI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspLU11I BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HpaI KpnI KspI MaeII MamI MfeI MluI MseI MunI MvnI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tsp509I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 545 TCCGGA T^CCGGA AfaI 765 GTAC GT^AC AlwNI 43 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 393 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 157 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 638 TGCGCA TGC^GCA AvrII 777 CCTAGG C^CTAGG BbeI 14 GGCGCC GGCGC^C BlnI 777 CCTAGG C^CTAGG BsaWI 545 TCCGGA [AT]^CCGG[AT] BseAI 545 TCCGGA T^CCGGA BseRI 901 CTCCTC GAGGAG(10/8) Bsp1407I 764 TGTACA T^GTACA BspEI 545 TCCGGA T^CCGGA BspHI 101 TCATGA T^CATGA BsrFI 1044 GCCGGC [GA]^CCGG[CT] BsrGI 764 TGTACA T^GTACA BssSI 19 CTCGTG CACGAG(-5/-1) Cfr10I 1044 GCCGGC [GA]^CCGG[CT] DraIII 445 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 965 CCATGG C^C[GA][CT]GG EarI 1009 CTCTTC CTCTTC(1/4) EcoNI 36 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 638 TGCGCA TGC^GCA HincII 949 GTCAAC GT[CT]^[GA]AC HindII 949 GTCAAC GT[CT]^[GA]AC HindIII 119 AAGCTT A^AGCTT HinfI 157 GACTC G^A[ACGT]TC KasI 14 GGCGCC G^GCGCC Ksp632I 1009 CTCTTC CTCTTC(1/4) MboII 1010 TCTTC GAAGA(8/7) MroI 545 TCCGGA T^CCGGA NaeI 1044 GCCGGC GCC^GGC NarI 14 GGCGCC GG^CGCC NcoI 965 CCATGG C^CATGG NgoMIV 1044 GCCGGC G^CCGGC PflFI 157 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 444 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 157 GACTC GAGTC(4/5) PpuMI 740 AGGACCT [GA]G^G[AT]CC[CT] RcaI 101 TCATGA T^CATGA RsaI 765 GTAC GT^AC SexAI 446 ACCTGGT A^CC[AT]GGT SfoI 14 GGCGCC GGC^GCC SmlI 895 CTCAAG C^T[CT][GA]AG SspBI 764 TGTACA T^GTACA StuI 395 AGGCCT AGG^CCT Tth111I 157 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 444 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 393 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 14 GGCGCC G[GA]^CG[CT]C 142 GGCGTC AlwI 384 GATCC GGATC(4/5) 456 GATCC AocI 137 CCTCAGG CC^T[ACGT]AGG 878 CCTCAGG BalI 866 TGGCCA TGG^CCA 1076 TGGCCA BcnI 153 CCCGG CC^[GC]GG 837 CCGGG BfaI 706 CTAG C^TAG 778 CTAG BglI 6 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 876 GCCCTCAGGGC BglII 260 AGATCT A^GATCT 374 AGATCT BsaHI 14 GGCGCC G[GA]^CG[CT]C 142 GGCGTC BsaI 685 GAGACC GGTCTC(1/5) 334 GGTCTC BsgI 919 CTGCAC GTGCAG(16/14) 1039 GTGCAG BsmFI 74 GGGAC GGGAC(10/14) 962 GTCCC Bsu36I 137 CCTCAGG CC^T[ACGT]AGG 878 CCTCAGG DraII 44 AGGCCCT [GA]G^G[ACGT]CC[CT] 740 AGGACCT Eco81I 137 CCTCAGG CC^T[ACGT]AGG 878 CCTCAGG EcoO109I 44 AGGCCCT [GA]G^G[ACGT]CC[CT] 740 AGGACCT HgaI 143 GCGTC GACGC(5/10) 1061 GCGTC Hin1I 14 GGCGCC G[GA]^CG[CT]C 142 GGCGTC MaeI 706 CTAG C^TAG 778 CTAG MaeIII 163 GTCAC ^GT[ACGT]AC 1063 GTCAC MluNI 866 TGGCCA TGG^CCA 1076 TGGCCA MscI 866 TGGCCA TGG^CCA 1076 TGGCCA MslI 561 CATCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 936 CACCAAAGTG MspA1I 537 CAGCGG C[AC]G^C[GT]G 1056 CCGCTG NciI 153 CCCGG CC^[GC]GG 837 CCGGG PshAI 232 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 955 GACTTCTGTC PstI 289 CTGCAG CTGCA^G 418 CTGCAG Tsp45I 163 GTCAC ^GT[GC]AC 1063 GTCAC XcmI 437 CCAAGAACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 473 CCAGCTCCCTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AluI AspHI AvaII BanI BanII BbvI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI TspRI TthHB8I XhoII