Restriction map for
pFN21AB8314
(Linear) (Six-base) MAPSORT of: KIBB8314.seq Check: 7817 from: 1 to: 1104
KIBB8314.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 218 1104
Size: 218 886
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 384 643 1104
Size: 262 122 259 461
Fragments arranged by size:
461 262 259 122
AleI CACnn'nnGTG
Cuts at: 0 940 1104
Size: 940 164
AlwNI CAG_nnn'CTG
Cuts at: 0 48 1104
Size: 48 1056
AvrII C'CTAG_G
Cuts at: 0 777 1104
Size: 777 327
BanI G'GyrC_C
Cuts at: 0 14 519 873 1104
Size: 14 505 354 231
Fragments arranged by size:
505 354 231 14
BanII G_rGCy'C
Cuts at: 0 314 491 545 845 887 1104
Size: 314 177 54 300 42 217
Fragments arranged by size:
314 300 217 177 54 42
BbeI G_GCGC'C
Cuts at: 0 18 1104
Size: 18 1086
BglI GCCn_nnn'nGGC
Cuts at: 0 12 882 1104
Size: 12 870 222
Fragments arranged by size:
870 222 12
BglII A'GATC_T
Cuts at: 0 260 374 1104
Size: 260 114 730
Fragments arranged by size:
730 260 114
Bme1580I G_kGCm'C
Cuts at: 0 254 524 878 1028 1104
Size: 254 270 354 150 76
Fragments arranged by size:
354 270 254 150 76
BmrI ACTGGGnnnn_n'
Cuts at: 0 1041 1104
Size: 1041 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 604 626 757 889 1104
Size: 322 282 22 131 132 215
Fragments arranged by size:
322 282 215 132 131 22
Bpu10I CC'TnA_GC
Cuts at: 0 37 817 1104
Size: 37 780 287
Fragments arranged by size:
780 287 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1104
Size: 880 224
BsaI GGTCTCn'nnnn_
Cuts at: 0 340 679 1104
Size: 340 339 425
Fragments arranged by size:
425 340 339
BsaHI Gr'CG_yC
Cuts at: 0 15 143 1104
Size: 15 128 961
Fragments arranged by size:
961 128 15
BsaWI w'CCGG_w
Cuts at: 0 545 1104
Size: 545 559
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 289 319 361 391 1104
Size: 289 30 42 30 713
Fragments arranged by size:
713 289 42 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 892 1104
Size: 892 212
BseYI C'CCAG_C
Cuts at: 0 472 771 1104
Size: 472 299 333
Fragments arranged by size:
472 333 299
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1060 1104
Size: 904 156 44
Fragments arranged by size:
904 156 44
BsiHKAI G_wGCw'C
Cuts at: 0 229 314 491 845 1104
Size: 229 85 177 354 259
Fragments arranged by size:
354 259 229 177 85
Bsp1286I G_dGCh'C
Cuts at: 0 229 254 314 491 524 545 845 878
Size: 229 25 60 177 33 21 300 33
Cuts at: 878 887 1028 1104
Size: 9 141 76
Fragments arranged by size:
300 229 177 141 76 60 33 33
25 21 9
BspEI T'CCGG_A
Cuts at: 0 545 1104
Size: 545 559
BspHI T'CATG_A
Cuts at: 0 101 1104
Size: 101 1003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 218 296 644 752 1104
Size: 218 78 348 108 352
Fragments arranged by size:
352 348 218 108 78
BsrFI r'CCGG_y
Cuts at: 0 1044 1104
Size: 1044 60
BsrGI T'GTAC_A
Cuts at: 0 764 1104
Size: 764 340
BssSI C'ACGA_G
Cuts at: 0 19 1104
Size: 19 1085
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 480 735 1104
Size: 282 198 255 369
Fragments arranged by size:
369 282 255 198
BstYI r'GATC_y
Cuts at: 0 260 374 383 455 1104
Size: 260 114 9 72 649
Fragments arranged by size:
649 260 114 72 9
Bsu36I CC'TnA_GG
Cuts at: 0 138 879 1104
Size: 138 741 225
Fragments arranged by size:
741 225 138
BtgI C'CryG_G
Cuts at: 0 965 1104
Size: 965 139
BtsI GCAGTG_nn'
Cuts at: 0 201 406 1104
Size: 201 205 698
Fragments arranged by size:
698 205 201
DraIII CAC_nnn'GTG
Cuts at: 0 450 1104
Size: 450 654
EaeI y'GGCC_r
Cuts at: 0 866 1046 1076 1104
Size: 866 180 30 28
Fragments arranged by size:
866 180 30 28
EarI CTCTTCn'nnn_
Cuts at: 0 1015 1104
Size: 1015 89
EcoICRI GAG'CTC
Cuts at: 0 312 489 843 1104
Size: 312 177 354 261
Fragments arranged by size:
354 312 261 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 322 384 604 626 643 757 889
Size: 262 60 62 220 22 17 114 132
Cuts at: 889 1104
Size: 215
Fragments arranged by size:
262 220 215 132 114 62 60 22
17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 40 1104
Size: 40 1064
EcoO109I rG'GnC_Cy
Cuts at: 0 45 741 1104
Size: 45 696 363
Fragments arranged by size:
696 363 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 342 1104
Size: 342 762
FspI TGC'GCA
Cuts at: 0 640 1104
Size: 640 464
HaeII r_GCGC'y
Cuts at: 0 18 131 182 1104
Size: 18 113 51 922
Fragments arranged by size:
922 113 51 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 1104
Size: 142 32 930
Fragments arranged by size:
930 142 32
HincII GTy'rAC
Cuts at: 0 951 1104
Size: 951 153
HindIII A'AGCT_T
Cuts at: 0 119 1104
Size: 119 985
KasI G'GCGC_C
Cuts at: 0 14 1104
Size: 14 1090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 950 1104
Size: 254 696 154
Fragments arranged by size:
696 254 154
MscI TGG'CCA
Cuts at: 0 868 1078 1104
Size: 868 210 26
Fragments arranged by size:
868 210 26
MslI CAynn'nnrTG
Cuts at: 0 565 940 1104
Size: 565 375 164
Fragments arranged by size:
565 375 164
MspA1I CmG'CkG
Cuts at: 0 539 1058 1104
Size: 539 519 46
Fragments arranged by size:
539 519 46
NaeI GCC'GGC
Cuts at: 0 1046 1104
Size: 1046 58
NarI GG'CG_CC
Cuts at: 0 15 1104
Size: 15 1089
NcoI C'CATG_G
Cuts at: 0 965 1104
Size: 965 139
NgoMIV G'CCGG_C
Cuts at: 0 1044 1104
Size: 1044 60
PflMI CCAn_nnn'nTGG
Cuts at: 0 450 1104
Size: 450 654
PfoI T'CCnGG_A
Cuts at: 0 146 836 1104
Size: 146 690 268
Fragments arranged by size:
690 268 146
PpuMI rG'GwC_Cy
Cuts at: 0 741 1104
Size: 741 363
PshAI GACnn'nnGTC
Cuts at: 0 236 959 1104
Size: 236 723 145
Fragments arranged by size:
723 236 145
PstI C_TGCA'G
Cuts at: 0 293 422 1104
Size: 293 129 682
Fragments arranged by size:
682 293 129
SacI G_AGCT'C
Cuts at: 0 314 491 845 1104
Size: 314 177 354 259
Fragments arranged by size:
354 314 259 177
SexAI A'CCwGG_T
Cuts at: 0 446 1104
Size: 446 658
SfcI C'TryA_G
Cuts at: 0 174 289 418 1104
Size: 174 115 129 686
Fragments arranged by size:
686 174 129 115
SfoI GGC'GCC
Cuts at: 0 16 1104
Size: 16 1088
SmlI C'TyrA_G
Cuts at: 0 895 1104
Size: 895 209
StuI AGG'CCT
Cuts at: 0 397 1104
Size: 397 707
StyI C'CwwG_G
Cuts at: 0 524 777 965 1104
Size: 524 253 188 139
Fragments arranged by size:
524 253 188 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 183 934 1104
Size: 183 751 170
Fragments arranged by size:
751 183 170
TatI w'GTAC_w
Cuts at: 0 764 1104
Size: 764 340
Tth111I GACn'n_nGTC
Cuts at: 0 160 1104
Size: 160 944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 444 480 1104
Size: 444 36 624
Fragments arranged by size:
624 444 36
XmnI GAAnn'nnTTC
Cuts at: 0 397 1104
Size: 397 707
Enzymes that do cut:
AarI AcuI AleI AlwNI AvrII BanI BanII BbeI
BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrFI BsrGI BssSI BstXI BstYI
Bsu36I BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV PflMI PfoI PpuMI PshAI PstI SacI SexAI
SfcI SfoI SmlI StuI StyI TaqII TatI Tth111I
XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BlpI BmgBI BmtI BplI BsaAI BsaBI
BsiEI BsiWI BsmI BsmBI BsrBI BsrDI BssHII BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI
EagI EciI EcoRI EcoRV FseI FspAI HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI XbaI XhoI
XmaI ZraI