Restriction map for pFN21AB8313
 (Linear) (Six-base) MAPSORT of: KIBB8313.seq  Check: 7399  from: 1  to: 1104



KIBB8313.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     557    1104 
   Size:        557     547
 
AccI GT'mk_AC

Cuts at:      0     854    1104 
   Size:        854     250
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154    1104 
   Size:        154     950
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     719     776    1104 
   Size:        719      57     328

  Fragments arranged by size: 

                 719     328      57
 
AlwNI CAG_nnn'CTG

Cuts at:      0     198     801     895     942     985    1104 
   Size:        198     603      94      47      43     119

  Fragments arranged by size: 

                 603     198     119      94      47      43
 
AvaI C'yCGr_G

Cuts at:      0     157     251     471    1104 
   Size:        157      94     220     633

  Fragments arranged by size: 

                 633     220     157      94
 
BanII G_rGCy'C

Cuts at:      0     377     533    1104 
   Size:        377     156     571

  Fragments arranged by size: 

                 571     377     156
 
BglI GCCn_nnn'nGGC

Cuts at:      0      62    1104 
   Size:         62    1042
 
BglII A'GATC_T

Cuts at:      0    1037    1104 
   Size:       1037      67
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     552    1104 
   Size:        552     552
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     447    1104 
   Size:        447     657
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364    1104 
   Size:        364     740
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1003    1104 
   Size:       1003     101
 
BsaWI w'CCGG_w

Cuts at:      0     440    1104 
   Size:        440     664
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     367     595     673     676     679    1104 
   Size:        367     228      78       3       3     425

  Fragments arranged by size: 

                 425     367     228      78       3       3
 
BseYI C'CCAG_C

Cuts at:      0     333     522     867    1043    1104 
   Size:        333     189     345     176      61

  Fragments arranged by size: 

                 345     333     189     176      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     225    1104 
   Size:        225     879
 
BsiEI CG_ry'CG

Cuts at:      0      57    1104 
   Size:         57    1047
 
BsiHKAI G_wGCw'C

Cuts at:      0     377     463     488     533    1104 
   Size:        377      86      25      45     571

  Fragments arranged by size: 

                 571     377      86      45      25
 
Bsp1286I G_dGCh'C

Cuts at:      0     377     463     488     533    1104 
   Size:        377      86      25      45     571

  Fragments arranged by size: 

                 571     377      86      45      25
 
BspEI T'CCGG_A

Cuts at:      0     440    1104 
   Size:        440     664
 
BspHI T'CATG_A

Cuts at:      0    1064    1104 
   Size:       1064      40
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     557    1104 
   Size:        557     547
 
BssSI C'ACGA_G

Cuts at:      0     340    1104 
   Size:        340     764
 
BstYI r'GATC_y

Cuts at:      0     230     314    1037    1104 
   Size:        230      84     723      67

  Fragments arranged by size: 

                 723     230      84      67
 
BtgI C'CryG_G

Cuts at:      0      57     580    1004    1104 
   Size:         57     523     424     100

  Fragments arranged by size: 

                 523     424     100      57
 
BtsI GCAGTG_nn'

Cuts at:      0     265     637    1104 
   Size:        265     372     467

  Fragments arranged by size: 

                 467     372     265
 
EaeI y'GGCC_r

Cuts at:      0      54    1104 
   Size:         54    1050
 
EagI C'GGCC_G

Cuts at:      0      54    1104 
   Size:         54    1050
 
EarI CTCTTCn'nnn_

Cuts at:      0     538    1104 
   Size:        538     566
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     691    1104 
   Size:        691     413
 
EcoICRI GAG'CTC

Cuts at:      0     375     531    1104 
   Size:        375     156     573

  Fragments arranged by size: 

                 573     375     156
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     447    1104 
   Size:        154     293     657

  Fragments arranged by size: 

                 657     293     154
 
EcoO109I rG'GnC_Cy

Cuts at:      0     138     297     892    1104 
   Size:        138     159     595     212

  Fragments arranged by size: 

                 595     212     159     138
 
HaeII r_GCGC'y

Cuts at:      0     888    1104 
   Size:        888     216
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      58      90    1104 
   Size:         58      32    1014

  Fragments arranged by size: 

                1014      58      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     842    1104 
   Size:        842     262
 
MspA1I CmG'CkG

Cuts at:      0     693    1104 
   Size:        693     411
 
NspI r_CATG'y

Cuts at:      0     597    1104 
   Size:        597     507
 
PpuMI rG'GwC_Cy

Cuts at:      0     138     297    1104 
   Size:        138     159     807

  Fragments arranged by size: 

                 807     159     138
 
PspXI vC'TCGA_Gb

Cuts at:      0     157     471    1104 
   Size:        157     314     633

  Fragments arranged by size: 

                 633     314     157
 
PstI C_TGCA'G

Cuts at:      0     272     554     698    1104 
   Size:        272     282     144     406

  Fragments arranged by size: 

                 406     282     272     144
 
PvuII CAG'CTG

Cuts at:      0     693    1104 
   Size:        693     411
 
SacI G_AGCT'C

Cuts at:      0     377     533    1104 
   Size:        377     156     571

  Fragments arranged by size: 

                 571     377     156
 
SanDI GG'GwC_CC

Cuts at:      0     297    1104 
   Size:        297     807
 
SapI GCTCTTCn'nnn_

Cuts at:      0     538    1104 
   Size:        538     566
 
ScaI AGT'ACT

Cuts at:      0     736    1104 
   Size:        736     368
 
SfcI C'TryA_G

Cuts at:      0     268     550     694     951    1104 
   Size:        268     282     144     257     153

  Fragments arranged by size: 

                 282     268     257     153     144
 
SmaI CCC'GGG

Cuts at:      0     253    1104 
   Size:        253     851
 
SmlI C'TyrA_G

Cuts at:      0     157     379     471    1104 
   Size:        157     222      92     633

  Fragments arranged by size: 

                 633     222     157      92
 
StuI AGG'CCT

Cuts at:      0     517    1104 
   Size:        517     587
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     446    1104 
   Size:        446     658
 
TatI w'GTAC_w

Cuts at:      0     734    1104 
   Size:        734     370
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     187     219    1104 
   Size:        187      32     885

  Fragments arranged by size: 

                 885     187      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     111    1104 
   Size:        111     993
 
XhoI C'TCGA_G

Cuts at:      0     157     471    1104 
   Size:        157     314     633

  Fragments arranged by size: 

                 633     314     157
 
XmaI C'CCGG_G

Cuts at:      0     251    1104 
   Size:        251     853
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AlfI    AlwNI     AvaI    BanII     BglI
    BglII     BplI     BpmI    BpuEI     BsaI    BsaWI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BssSI
    BstYI     BtgI     BtsI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    HaeII    Hin4I     MmeI   MspA1I     NspI    PpuMI
    PspXI     PstI    PvuII     SacI    SanDI     SapI     ScaI     SfcI
     SmaI     SmlI     StuI    TaqII     TatI     TstI     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmrI     BmtI
   Bpu10I    BsaAI    BsaBI    BsaHI    BsaXI    BsiWI     BsmI    BsmBI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI     PvuI    RsrII
    SacII     SalI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI  Tth111I
     XbaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com