Restriction map for
pFN21AB8305
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BsaBI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeI MamI MluI
MluNI MscI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 812 TCCGGA T^CCGGA
Alw44I 940 GTGCAC G^TGCAC
AlwNI 1507 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 940 GTGCAC G^TGCAC
AvaI 565 CCCGAG C^[CT]CG[GA]G
BamHI 735 GGATCC G^GATCC
BbrPI 931 CACGTG CAC^GTG
BbsI 1401 GAAGAC GAAGAC(2/6)
BclI 1706 TGATCA T^GATCA
BlpI 146 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 146 GCTCAGC GC^T[ACGT]AGC
BpuAI 1401 GAAGAC GAAGAC(2/6)
BsaAI 931 CACGTG [CT]AC^GT[GA]
BseAI 812 TCCGGA T^CCGGA
BseRI 1933 GAGGAG GAGGAG(10/8)
BsmI 1116 GAATGC GAATGC(1/-1)
BsoBI 565 CCCGAG C^[CT]CG[GA]G
BspEI 812 TCCGGA T^CCGGA
BspHI 29 TCATGA T^CATGA
BsrDI 657 CATTGC GCAATG(2/0)
BsrFI 275 GCCGGC [GA]^CCGG[CT]
BstAPI 723 GCAGTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 146 GCTCAGC GC^T[ACGT]AGC
Cfr10I 275 GCCGGC [GA]^CCGG[CT]
DraII 670 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 304 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 892 GACAAAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 670 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 782 CCTTGG C^C[AT][AT]GG
FauI 1386 GCGGG CCCGC(4/6)
FbaI 1706 TGATCA T^GATCA
HgaI 990 GCGTC GACGC(5/10)
MfeI 401 CAATTG C^AATTG
MroI 812 TCCGGA T^CCGGA
MseI 1562 TTAA T^TAA
MunI 401 CAATTG C^AATTG
NaeI 275 GCCGGC GCC^GGC
NgoMIV 275 GCCGGC G^CCGGC
PleI 1585 GACTC GAGTC(4/5)
PmaCI 931 CACGTG CAC^GTG
PmlI 931 CACGTG CAC^GTG
PpuMI 670 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 103 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1633 CTGCAG CTGCA^G
RcaI 29 TCATGA T^CATGA
SacI 358 GAGCTC GAGCT^C
ScaI 1370 AGTACT AGT^ACT
SmlI 423 CTCAAG C^T[CT][GA]AG
StyI 782 CCTTGG C^C[AT][AT]GG
TaqI 239 TCGA T^CGA
TfiI 230 GAATC G^A[AT]TC
Tru9I 1562 TTAA T^TAA
TthHB8I 239 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 62 GACGTC GACGT^C
223 GACGTC
AccII 1393 CGCG CG^CG
1502 CGCG
AcsI 211 AAATTT [GA]^AATT[CT]
756 AAATTC
AflIII 932 ACGTGT A^C[GA][CT]GT
1739 ACGTGT
ApoI 211 AAATTT [GA]^AATT[CT]
756 AAATTC
BbeI 1091 GGCGCC GGCGC^C
1178 GGCGCC
BbvCI 1849 CCTCAGC CCTCAGC(-5/-2)
1930 GCTGAGG
BsaWI 68 ACCGGA [AT]^CCGG[AT]
812 TCCGGA
BspMI 1529 ACCTGC ACCTGC(4/8)
1266 GCAGGT
BsrBI 1029 CCGCTC CCGCTC(-3/-3)
643 GAGCGG
BstUI 1393 CGCG CG^CG
1502 CGCG
BstXI 600 CCAGAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1412 CCATCTTCATGG
DsaI 1086 CCGTGG C^C[GA][CT]GG
1789 CCGTGG
HinfI 230 GAATC G^A[ACGT]TC
1585 GACTC
KasI 1091 GGCGCC G^GCGCC
1178 GGCGCC
MvnI 1393 CGCG CG^CG
1502 CGCG
NarI 1091 GGCGCC GG^CGCC
1178 GGCGCC
PflMI 777 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1169 CCATCAACTGG
PvuII 1852 CAGCTG CAG^CTG
1928 CAGCTG
SfcI 954 CTACAG C^T[GA][CT]AG
1633 CTGCAG
SfoI 1091 GGCGCC GGC^GCC
1178 GGCGCC
SphI 728 GCATGC GCATG^C
1661 GCATGC
Van91I 777 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1169 CCATCAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AocI AspHI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
EaeI Eco81I EcoNI EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I Tsp509I TspRI
XhoII