(Circular) (Six-base) MAPSORT of: pFN21AB8305.seq Check: 9608 from: 1 to: 6676 pFN21AB8305 6676 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2071 2232 4486 278 Size: 53 83 186 936 535 161 2254 2468 Fragments arranged by size: 2468 2254 936 535 186 161 83 53 AccI GT'mk_AC Cuts at: 1937 4019 1937 Size: 2082 4594 Acc65I G'GTAC_C Cuts at: 3997 3997 Size: 6676 AclI AA'CG_TT Cuts at: 1563 4803 5176 1563 Size: 3240 373 3063 Fragments arranged by size: 3240 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3915 4739 6123 6391 1632 Size: 2283 824 1384 268 1917 Fragments arranged by size: 2283 1917 1384 824 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6479 AflIII A'CryG_T Cuts at: 1203 2937 3744 5575 6552 1203 Size: 1734 807 1831 977 1327 Fragments arranged by size: 1831 1734 1327 977 807 AgeI A'CCGG_T Cuts at: 5507 5507 Size: 6676 AhdI GACnn_n'nnGTC Cuts at: 5405 5405 Size: 6676 AleI CACnn'nnGTG Cuts at: 1166 3020 3767 1166 Size: 1854 747 4075 Fragments arranged by size: 4075 1854 747 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2679 3653 1184 Size: 1495 974 4207 Fragments arranged by size: 4207 1495 974 AlwNI CAG_nnn'CTG Cuts at: 3517 4419 5991 3517 Size: 902 1572 4202 Fragments arranged by size: 4202 1572 902 ApaLI G'TGCA_C Cuts at: 2945 4733 5889 2945 Size: 1788 1156 3732 Fragments arranged by size: 3732 1788 1156 ApoI r'AATT_y Cuts at: 1493 2216 2761 3985 4211 4363 6472 1493 Size: 723 545 1224 226 152 2109 1697 Fragments arranged by size: 2109 1697 1224 723 545 226 152 AseI AT'TA_AT Cuts at: 160 1032 5230 160 Size: 872 4198 1606 Fragments arranged by size: 4198 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 AvaI C'yCGr_G Cuts at: 1870 1943 2570 4001 1870 Size: 73 627 1431 4545 Fragments arranged by size: 4545 1431 627 73 BamHI G'GATC_C Cuts at: 2740 4006 4373 2740 Size: 1266 367 5043 Fragments arranged by size: 5043 1266 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2586 3022 3096 3183 3209 Size: 325 217 627 799 436 74 87 26 Cuts at: 3209 3997 5452 618 Size: 788 1455 1842 Fragments arranged by size: 1842 1455 799 788 627 436 325 217 87 74 26 BanII G_rGCy'C Cuts at: 1391 2367 2577 3446 3704 3995 1391 Size: 976 210 869 258 291 4072 Fragments arranged by size: 4072 976 869 291 258 210 BbeI G_GCGC'C Cuts at: 3100 3187 3100 Size: 87 6589 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3413 928 Size: 971 1514 4191 Fragments arranged by size: 4191 1514 971 BbvCI CC'TCA_GC Cuts at: 3855 3936 3855 Size: 81 6595 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4867 4901 1903 Size: 34 2930 34 3678 Fragments arranged by size: 3678 2930 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4568 5778 4568 Size: 1210 5466 BclI T'GATC_A Cuts at: 1548 3711 1548 Size: 2163 4513 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5287 136 Size: 107 122 71 631 605 3615 1525 Fragments arranged by size: 3615 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6671 6671 Size: 6676 BlpI GC'TnA_GC Cuts at: 2152 4093 2152 Size: 1941 4735 Bme1580I G_kGCm'C Cuts at: 1790 2842 2949 3027 3172 4737 5893 1790 Size: 1052 107 78 145 1565 1156 2573 Fragments arranged by size: 2573 1565 1156 1052 145 107 78 BmgBI CAC'GTC Cuts at: 1318 3241 3254 3745 1318 Size: 1923 13 491 4249 Fragments arranged by size: 4249 1923 491 13 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2527 3159 4407 Size: 416 53 83 399 24 1110 632 1248 Cuts at: 4407 5365 442 Size: 958 1753 Fragments arranged by size: 1753 1248 1110 958 632 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6676 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 3013 3344 3539 5336 1239 Size: 800 974 331 195 1797 2579 Fragments arranged by size: 2579 1797 974 800 331 195 Bpu10I CC'TnA_GC Cuts at: 2646 2747 3041 3855 3936 2646 Size: 101 294 814 81 5386 Fragments arranged by size: 5386 814 294 101 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2413 4801 5666 5964 2413 Size: 2388 865 298 3125 Fragments arranged by size: 3125 2388 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2084 2711 3919 5339 882 Size: 617 585 627 1208 1420 2219 Fragments arranged by size: 2219 1420 1208 627 617 585 BsaAI yAC'GTr Cuts at: 493 1204 2938 4467 493 Size: 711 1734 1529 2702 Fragments arranged by size: 2702 1734 1529 711 BsaBI GATnn'nnATC Cuts at: 6670 6670 Size: 6676 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2068 2229 2995 3097 Size: 53 83 186 936 535 161 766 102 Cuts at: 3097 3184 4483 4865 5500 275 Size: 87 1299 382 635 1451 Fragments arranged by size: 1451 1299 936 766 635 535 382 186 161 102 87 83 53 BsaWI w'CCGG_w Cuts at: 2073 2817 5109 5507 5781 5928 6339 2073 Size: 744 2292 398 274 147 411 2410 Fragments arranged by size: 2410 2292 744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3145 3175 3512 3542 1178 Size: 30 165 30 1742 30 337 30 4312 Fragments arranged by size: 4312 1742 337 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3953 1186 Size: 2767 3909 BseYI C'CCAG_C Cuts at: 3596 3610 3840 5879 6375 3596 Size: 14 230 2039 496 3897 Fragments arranged by size: 3897 2039 496 230 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2214 2775 3155 3392 3521 3745 1163 Size: 573 478 561 380 237 129 224 4094 Fragments arranged by size: 4094 573 561 478 380 237 224 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4126 4887 5915 1540 Size: 275 186 2125 761 1028 2301 Fragments arranged by size: 2301 2125 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2250 2310 2367 2949 3047 3322 3478 4737 4822 Size: 60 57 582 98 275 156 1259 85 Cuts at: 4822 5893 2250 Size: 1071 3033 Fragments arranged by size: 3033 1259 1071 582 275 156 98 85 60 57 BsmI GAATG_Cn' Cuts at: 3127 4187 4280 3127 Size: 1060 93 5523 Fragments arranged by size: 5523 1060 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2310 2367 2577 2842 2949 3027 Size: 399 460 60 57 210 265 107 78 Cuts at: 3027 3047 3172 3322 3446 3478 3704 3995 4737 Size: 20 125 150 124 32 226 291 742 Cuts at: 4737 4822 5893 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 460 399 291 265 226 210 150 125 124 107 85 78 60 57 32 20 BspEI T'CCGG_A Cuts at: 2817 6339 2817 Size: 3522 3154 BspHI T'CATG_A Cuts at: 2034 4565 6295 2034 Size: 2531 1730 2415 Fragments arranged by size: 2531 2415 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3262 3543 4031 844 Size: 2418 281 488 3489 Fragments arranged by size: 3489 2418 488 281 BsrBI CCG'CTC Cuts at: 1394 1823 2650 3036 4563 1394 Size: 429 827 386 1527 3507 Fragments arranged by size: 3507 1527 827 429 386 BsrDI GCAATG_nn' Cuts at: 65 1248 2661 5171 5345 65 Size: 1183 1413 2510 174 1396 Fragments arranged by size: 2510 1413 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2280 5320 5507 1700 Size: 110 470 3040 187 2869 Fragments arranged by size: 3040 2869 470 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6676 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6676 BssSI C'ACGA_G Cuts at: 4736 5748 4736 Size: 1012 5664 BstAPI GCAn_nnn'nTGC Cuts at: 794 2734 794 Size: 1940 4736 BstBI TT'CG_AA Cuts at: 5480 5480 Size: 6676 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6676 BstXI CCAn_nnnn'nTGG Cuts at: 2612 3424 2612 Size: 812 5864 BstYI r'GATC_y Cuts at: 1972 2740 3333 3513 4006 4373 4758 4775 6227 Size: 768 593 180 493 367 385 17 1452 Cuts at: 6227 6313 6325 6671 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 768 593 493 385 367 346 180 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2444 3705 3896 2444 Size: 1261 191 5224 Fragments arranged by size: 5224 1261 191 BtgI C'CryG_G Cuts at: 513 1065 2004 3091 3794 513 Size: 552 939 1087 703 3395 Fragments arranged by size: 3395 1087 939 703 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5937 Fragments arranged by size: 5937 683 56 BtsI GCAGTG_nn' Cuts at: 2735 2758 4192 4986 5006 2735 Size: 23 1434 794 20 4405 Fragments arranged by size: 4405 1434 794 23 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6676 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6641 DraI TTT'AAA Cuts at: 3980 3980 Size: 6676 DraIII CAC_nnn'GTG Cuts at: 2314 2314 Size: 6676 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2903 5683 809 Size: 507 1587 2780 1802 Fragments arranged by size: 2780 1802 1587 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2614 3816 3883 Size: 54 1425 50 275 18 784 1202 67 Cuts at: 3883 4123 5012 6641 8 Size: 240 889 1629 43 Fragments arranged by size: 1629 1425 1202 889 784 275 240 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4123 1537 Size: 275 2311 4090 Fragments arranged by size: 4090 2311 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4606 1353 Size: 3253 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5263 5637 5783 387 Size: 1064 138 3674 374 146 1280 Fragments arranged by size: 3674 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2365 2365 Size: 6676 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 3013 3344 3539 3915 4739 5336 Size: 393 407 974 331 195 376 824 597 Cuts at: 5336 6123 6391 1239 Size: 787 268 1524 Fragments arranged by size: 1524 974 824 787 597 407 393 376 331 268 195 EcoNI CCTnn'n_nnAGG Cuts at: 2791 3203 3217 2791 Size: 412 14 6250 Fragments arranged by size: 6250 412 14 EcoO109I rG'GnC_Cy Cuts at: 2676 2676 Size: 6676 EcoRI G'AATT_C Cuts at: 3985 4363 3985 Size: 378 6298 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2411 2411 Size: 6676 FspI TGC'GCA Cuts at: 5182 5182 Size: 6676 HaeII r_GCGC'y Cuts at: 1678 2932 3100 3187 5823 1678 Size: 1254 168 87 2636 2531 Fragments arranged by size: 2636 2531 1254 168 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2411 2443 2795 2827 3512 Size: 32 135 32 839 32 352 32 685 Cuts at: 3512 3544 3564 3596 5317 5349 5391 5423 1373 Size: 32 20 32 1721 32 42 32 2626 Fragments arranged by size: 2626 1721 839 685 352 135 42 32 32 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 4020 4266 669 Size: 988 281 2082 246 3079 Fragments arranged by size: 3079 2082 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6676 HpaI GTT'AAC Cuts at: 4266 4266 Size: 6676 KasI G'GCGC_C Cuts at: 3096 3183 3096 Size: 87 6589 KpnI G_GTAC'C Cuts at: 4001 4001 Size: 6676 MfeI C'AATT_G Cuts at: 2406 4275 2406 Size: 1869 4807 MluI A'CGCG_T Cuts at: 6552 6552 Size: 6676 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5790 5974 1305 Size: 4485 184 2007 Fragments arranged by size: 4485 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4854 Fragments arranged by size: 4854 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3020 3422 3767 3908 4634 4993 5152 Size: 648 1854 402 345 141 726 359 159 Cuts at: 5152 518 Size: 2042 Fragments arranged by size: 2042 1854 726 648 402 359 345 159 141 MspA1I CmG'CkG Cuts at: 1606 3717 3859 3935 4092 4769 5917 6162 1606 Size: 2111 142 76 157 677 1148 245 2120 Fragments arranged by size: 2120 2111 1148 677 245 157 142 76 NaeI GCC'GGC Cuts at: 2282 2282 Size: 6676 NarI GG'CG_CC Cuts at: 3097 3184 3097 Size: 87 6589 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5185 Fragments arranged by size: 5185 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6676 NgoMIV G'CCGG_C Cuts at: 2280 2280 Size: 6676 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6676 NotI GC'GGCC_GC Cuts at: 4123 4123 Size: 6676 NspI r_CATG'y Cuts at: 1132 1229 2737 3436 3529 3670 4034 5579 1132 Size: 97 1508 699 93 141 364 1545 2229 Fragments arranged by size: 2229 1545 1508 699 364 141 97 93 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6676 PciI A'CATG_T Cuts at: 5575 5575 Size: 6676 PflMI CCAn_nnn'nTGG Cuts at: 1108 2788 3180 1108 Size: 1680 392 4604 Fragments arranged by size: 4604 1680 392 PfoI T'CCnGG_A Cuts at: 3516 3516 Size: 6676 PmeI GTTT'AAAC Cuts at: 3980 3980 Size: 6676 PmlI CAC'GTG Cuts at: 2938 2938 Size: 6676 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2690 2722 4745 4777 6258 6290 2690 Size: 32 2023 32 1481 32 3076 Fragments arranged by size: 3076 2023 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2676 2676 Size: 6676 PshAI GACnn'nnGTC Cuts at: 1613 2112 1613 Size: 499 6177 PsiI TTA'TAA Cuts at: 4246 4246 Size: 6676 PstI C_TGCA'G Cuts at: 830 3642 4028 830 Size: 2812 386 3478 Fragments arranged by size: 3478 2812 386 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6676 PvuII CAG'CTG Cuts at: 3859 3935 3859 Size: 76 6600 SacI G_AGCT'C Cuts at: 2367 2367 Size: 6676 SalI G'TCGA_C Cuts at: 1936 4018 1936 Size: 2082 4594 SbfI CC_TGCA'GG Cuts at: 4028 4028 Size: 6676 ScaI AGT'ACT Cuts at: 3377 4924 6636 3377 Size: 1547 1712 3417 Fragments arranged by size: 3417 1712 1547 SfcI C'TryA_G Cuts at: 826 1045 2959 3638 4024 5159 5840 6031 826 Size: 219 1914 679 386 1135 681 191 1471 Fragments arranged by size: 1914 1471 1135 681 679 386 219 191 SfoI GGC'GCC Cuts at: 3098 3185 3098 Size: 87 6589 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6676 SmaI CCC'GGG Cuts at: 1872 4003 1872 Size: 2131 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2428 4780 5681 5943 820 Size: 197 926 485 2352 901 262 1553 Fragments arranged by size: 2352 1553 926 901 485 262 197 SnaBI TAC'GTA Cuts at: 493 4467 493 Size: 3974 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6676 SphI G_CATG'C Cuts at: 1132 2737 3670 4034 1132 Size: 1605 933 364 3774 Fragments arranged by size: 3774 1605 933 364 SspI AAT'ATT Cuts at: 5 52 4600 5 Size: 47 4548 2081 Fragments arranged by size: 4548 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6676 StyI C'CwwG_G Cuts at: 513 1065 2004 2787 4115 513 Size: 552 939 783 1328 3074 Fragments arranged by size: 3074 1328 939 783 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3854 4703 4720 4873 5058 5503 325 Size: 1255 2274 849 17 153 185 445 1498 Fragments arranged by size: 2274 1498 1255 849 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3375 3429 3501 Size: 274 80 33 51 1443 1398 54 72 Cuts at: 3501 4922 6634 96 Size: 1421 1712 138 Fragments arranged by size: 1712 1443 1421 1398 274 138 80 72 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6636 6668 6636 Size: 32 6644 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6676 XbaI T'CTAG_A Cuts at: 4012 4012 Size: 6676 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6676 XmaI C'CCGG_G Cuts at: 1870 4001 1870 Size: 2131 4545 XmnI GAAnn'nnTTC Cuts at: 4805 4805 Size: 6676 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2069 2230 4484 276 Size: 53 83 186 936 535 161 2254 2468 Fragments arranged by size: 2468 2254 936 535 186 161 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI