Restriction map for
pFN21AB8305
(Linear) (Six-base) MAPSORT of: KIBB8305.seq Check: 6943 from: 1 to: 1971
KIBB8305.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AatII G_ACGT'C
Cuts at: 0 66 227 1971
Size: 66 161 1744
Fragments arranged by size:
1744 161 66
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1910 1971
Size: 1910 61
AflIII A'CryG_T
Cuts at: 0 932 1739 1971
Size: 932 807 232
Fragments arranged by size:
932 807 232
AleI CACnn'nnGTG
Cuts at: 0 1015 1762 1971
Size: 1015 747 209
Fragments arranged by size:
1015 747 209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 674 1648 1971
Size: 674 974 323
Fragments arranged by size:
974 674 323
AlwNI CAG_nnn'CTG
Cuts at: 0 1512 1971
Size: 1512 459
ApaLI G'TGCA_C
Cuts at: 0 940 1971
Size: 940 1031
ApoI r'AATT_y
Cuts at: 0 211 756 1971
Size: 211 545 1215
Fragments arranged by size:
1215 545 211
AvaI C'yCGr_G
Cuts at: 0 565 1971
Size: 565 1406
BamHI G'GATC_C
Cuts at: 0 735 1971
Size: 735 1236
BanI G'GyrC_C
Cuts at: 0 581 1017 1091 1178 1204 1971
Size: 581 436 74 87 26 767
Fragments arranged by size:
767 581 436 87 74 26
BanII G_rGCy'C
Cuts at: 0 362 572 1441 1699 1971
Size: 362 210 869 258 272
Fragments arranged by size:
869 362 272 258 210
BbeI G_GCGC'C
Cuts at: 0 1095 1182 1971
Size: 1095 87 789
Fragments arranged by size:
1095 789 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 1408 1971
Size: 1408 563
BbvCI CC'TCA_GC
Cuts at: 0 1850 1931 1971
Size: 1850 81 40
Fragments arranged by size:
1850 81 40
BclI T'GATC_A
Cuts at: 0 1706 1971
Size: 1706 265
BlpI GC'TnA_GC
Cuts at: 0 147 1971
Size: 147 1824
Bme1580I G_kGCm'C
Cuts at: 0 837 944 1022 1167 1971
Size: 837 107 78 145 804
Fragments arranged by size:
837 804 145 107 78
BmgBI CAC'GTC
Cuts at: 0 1236 1249 1740 1971
Size: 1236 13 491 231
Fragments arranged by size:
1236 491 231 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 522 1154 1971
Size: 522 632 817
Fragments arranged by size:
817 632 522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1008 1339 1534 1971
Size: 34 974 331 195 437
Fragments arranged by size:
974 437 331 195 34
Bpu10I CC'TnA_GC
Cuts at: 0 641 742 1036 1850 1931 1971
Size: 641 101 294 814 81 40
Fragments arranged by size:
814 641 294 101 81 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 408 1971
Size: 408 1563
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 706 1914 1971
Size: 79 627 1208 57
Fragments arranged by size:
1208 627 79 57
BsaAI yAC'GTr
Cuts at: 0 933 1971
Size: 933 1038
BsaHI Gr'CG_yC
Cuts at: 0 63 224 990 1092 1179 1971
Size: 63 161 766 102 87 792
Fragments arranged by size:
792 766 161 102 87 63
BsaWI w'CCGG_w
Cuts at: 0 68 812 1971
Size: 68 744 1159
Fragments arranged by size:
1159 744 68
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1140 1170 1507 1537 1971
Size: 1140 30 337 30 434
Fragments arranged by size:
1140 434 337 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1948 1971
Size: 1948 23
BseYI C'CCAG_C
Cuts at: 0 1591 1605 1835 1971
Size: 1591 14 230 136
Fragments arranged by size:
1591 230 136 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 770 1150 1387 1516 1740 1971
Size: 209 561 380 237 129 224 231
Fragments arranged by size:
561 380 237 231 224 209 129
BsiHKAI G_wGCw'C
Cuts at: 0 245 305 362 944 1042 1317 1473 1971
Size: 245 60 57 582 98 275 156 498
Fragments arranged by size:
582 498 275 245 156 98 60 57
BsmI GAATG_Cn'
Cuts at: 0 1122 1971
Size: 1122 849
Bsp1286I G_dGCh'C
Cuts at: 0 245 305 362 572 837 944 1022 1042
Size: 245 60 57 210 265 107 78 20
Cuts at: 1042 1167 1317 1441 1473 1699 1971
Size: 125 150 124 32 226 272
Fragments arranged by size:
272 265 245 226 210 150 125 124
107 78 60 57 32 20
BspEI T'CCGG_A
Cuts at: 0 812 1971
Size: 812 1159
BspHI T'CATG_A
Cuts at: 0 29 1971
Size: 29 1942
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1257 1538 1971
Size: 1257 281 433
Fragments arranged by size:
1257 433 281
BsrBI CCG'CTC
Cuts at: 0 645 1031 1971
Size: 645 386 940
Fragments arranged by size:
940 645 386
BsrDI GCAATG_nn'
Cuts at: 0 656 1971
Size: 656 1315
BsrFI r'CCGG_y
Cuts at: 0 275 1971
Size: 275 1696
BstAPI GCAn_nnn'nTGC
Cuts at: 0 729 1971
Size: 729 1242
BstXI CCAn_nnnn'nTGG
Cuts at: 0 607 1419 1971
Size: 607 812 552
Fragments arranged by size:
812 607 552
BstYI r'GATC_y
Cuts at: 0 735 1328 1508 1971
Size: 735 593 180 463
Fragments arranged by size:
735 593 463 180
Bsu36I CC'TnA_GG
Cuts at: 0 439 1700 1891 1971
Size: 439 1261 191 80
Fragments arranged by size:
1261 439 191 80
BtgI C'CryG_G
Cuts at: 0 1086 1789 1971
Size: 1086 703 182
Fragments arranged by size:
1086 703 182
BtsI GCAGTG_nn'
Cuts at: 0 730 753 1971
Size: 730 23 1218
Fragments arranged by size:
1218 730 23
DraIII CAC_nnn'GTG
Cuts at: 0 309 1971
Size: 309 1662
DrdI GACnn_nn'nnGTC
Cuts at: 0 898 1971
Size: 898 1073
EaeI y'GGCC_r
Cuts at: 0 609 1811 1878 1971
Size: 609 1202 67 93
Fragments arranged by size:
1202 609 93 67
EcoICRI GAG'CTC
Cuts at: 0 360 1971
Size: 360 1611
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1008 1339 1534 1910 1971
Size: 34 974 331 195 376 61
Fragments arranged by size:
974 376 331 195 61 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 786 1198 1212 1971
Size: 786 412 14 759
Fragments arranged by size:
786 759 412 14
EcoO109I rG'GnC_Cy
Cuts at: 0 671 1971
Size: 671 1300
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 406 1971
Size: 406 1565
HaeII r_GCGC'y
Cuts at: 0 927 1095 1182 1971
Size: 927 168 87 789
Fragments arranged by size:
927 789 168 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 406 438 790 822 1507 1539 1559 1591
Size: 406 32 352 32 685 32 20 32
Cuts at: 1591 1971
Size: 380
Fragments arranged by size:
685 406 380 352 32 32 32 32
20
KasI G'GCGC_C
Cuts at: 0 1091 1178 1971
Size: 1091 87 793
Fragments arranged by size:
1091 793 87
MfeI C'AATT_G
Cuts at: 0 401 1971
Size: 401 1570
MslI CAynn'nnrTG
Cuts at: 0 1015 1417 1762 1903 1971
Size: 1015 402 345 141 68
Fragments arranged by size:
1015 402 345 141 68
MspA1I CmG'CkG
Cuts at: 0 1712 1854 1930 1971
Size: 1712 142 76 41
Fragments arranged by size:
1712 142 76 41
NaeI GCC'GGC
Cuts at: 0 277 1971
Size: 277 1694
NarI GG'CG_CC
Cuts at: 0 1092 1179 1971
Size: 1092 87 792
Fragments arranged by size:
1092 792 87
NgoMIV G'CCGG_C
Cuts at: 0 275 1971
Size: 275 1696
NspI r_CATG'y
Cuts at: 0 732 1431 1524 1665 1971
Size: 732 699 93 141 306
Fragments arranged by size:
732 699 306 141 93
PflMI CCAn_nnn'nTGG
Cuts at: 0 783 1175 1971
Size: 783 392 796
Fragments arranged by size:
796 783 392
PfoI T'CCnGG_A
Cuts at: 0 1511 1971
Size: 1511 460
PmlI CAC'GTG
Cuts at: 0 933 1971
Size: 933 1038
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 685 717 1971
Size: 685 32 1254
Fragments arranged by size:
1254 685 32
PpuMI rG'GwC_Cy
Cuts at: 0 671 1971
Size: 671 1300
PshAI GACnn'nnGTC
Cuts at: 0 107 1971
Size: 107 1864
PstI C_TGCA'G
Cuts at: 0 1637 1971
Size: 1637 334
PvuII CAG'CTG
Cuts at: 0 1854 1930 1971
Size: 1854 76 41
Fragments arranged by size:
1854 76 41
SacI G_AGCT'C
Cuts at: 0 362 1971
Size: 362 1609
ScaI AGT'ACT
Cuts at: 0 1372 1971
Size: 1372 599
SfcI C'TryA_G
Cuts at: 0 954 1633 1971
Size: 954 679 338
Fragments arranged by size:
954 679 338
SfoI GGC'GCC
Cuts at: 0 1093 1180 1971
Size: 1093 87 791
Fragments arranged by size:
1093 791 87
SmlI C'TyrA_G
Cuts at: 0 423 1971
Size: 423 1548
SphI G_CATG'C
Cuts at: 0 732 1665 1971
Size: 732 933 306
Fragments arranged by size:
933 732 306
StyI C'CwwG_G
Cuts at: 0 782 1971
Size: 782 1189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1849 1971
Size: 1849 122
TatI w'GTAC_w
Cuts at: 0 1370 1424 1496 1971
Size: 1370 54 72 475
Fragments arranged by size:
1370 475 72 54
ZraI GAC'GTC
Cuts at: 0 64 225 1971
Size: 64 161 1746
Fragments arranged by size:
1746 161 64
Enzymes that do cut:
AatII AcuI AflIII AleI AlfI AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
KasI MfeI MslI MspA1I NaeI NarI NgoMIV NspI
PflMI PfoI PmlI PpiI PpuMI PshAI PstI PvuII
SacI ScaI SfcI SfoI SmlI SphI StyI TaqII
TatI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvrII BaeI BcgI
BciVI BfrBI BglI BglII BmtI BplI BsaBI BsiEI
BsiWI BsmBI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI EagI EarI EciI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MluI MmeI MscI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI