Restriction map for
pFN21AB8276
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaII AvrII BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeI MamI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AspI 229 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 206 TGCGCA TGC^GCA
BalI 251 TGGCCA TGG^CCA
BamHI 356 GGATCC G^GATCC
BbsI 21 GAAGAC GAAGAC(2/6)
BglI 266 GCCGCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 363 GGGCAC G[AGT]GC[ACT]^C
BpuAI 21 GAAGAC GAAGAC(2/6)
BseRI 241 GAGGAG GAGGAG(10/8)
BsgI 132 CTGCAC GTGCAG(16/14)
BsiEI 264 CGGCCG CG[GA][CT]^CG
BsmAI 67 GAGAC GTCTC(1/5)
BsmBI 67 GAGACG CGTCTC(1/5)
Bsp1286I 363 GGGCAC G[AGT]GC[ACT]^C
BsrI 101 ACTGG ACTGG(1/-1)
BstXI 41 CCATGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 356 GGATCC [GA]^GATC[CT]
DdeI 29 CTCAG C^T[ACGT]AG
DraIII 82 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 264 CGGCCG C^GGCCG
EclXI 264 CGGCCG C^GGCCG
Eco52I 264 CGGCCG C^GGCCG
EcoT14I 254 CCAAGG C^C[AT][AT]GG
FauI 406 GCGGG CCCGC(4/6)
FspI 206 TGCGCA TGC^GCA
HaeII 316 GGCGCT [GA]GCGC^[CT]
HinfI 33 GAATC G^A[ACGT]TC
MaeIII 12 GTCAC ^GT[ACGT]AC
MflI 356 GGATCC [GA]^GATC[CT]
MluNI 251 TGGCCA TGG^CCA
MscI 251 TGGCCA TGG^CCA
MseI 113 TTAA T^TAA
NotI 263 GCGGCCGC GC^GGCCGC
PflFI 229 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 277 CTGCAG CTGCA^G
SexAI 83 ACCTGGT A^CC[AT]GGT
SfcI 277 CTGCAG C^T[GA][CT]AG
SfiI 265 GGCCGCGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 360 CCCGGG CCC^GGG
StuI 273 AGGCCT AGG^CCT
StyI 254 CCAAGG C^C[AT][AT]GG
TaqI 227 TCGA T^CGA
TfiI 33 GAATC G^A[AT]TC
Tru9I 113 TTAA T^TAA
Tsp45I 12 GTCAC ^GT[GC]AC
TspRI 14 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 229 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 227 TCGA T^CGA
XhoII 356 GGATCC [GA]^GATC[CT]
XmaCI 360 CCCGGG C^CCGGG
XmaI 360 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 72 GTAC GT^AC
201 GTAC
AluI 53 AGCT AG^CT
91 AGCT
AlwI 357 GATCC GGATC(4/5)
356 GGATC
AvaI 169 CCCGAG C^[CT]CG[GA]G
360 CCCGGG
BsmFI 143 GGGAC GGGAC(10/14)
149 GGGAC
BsoBI 169 CCCGAG C^[CT]CG[GA]G
360 CCCGGG
BspMI 116 ACCTGC ACCTGC(4/8)
203 ACCTGC
CfoI 207 GCGC GCG^C
317 GCGC
Cfr13I 258 GGGCC G^G[ACGT]CC
345 GGGCC
DpnII 222 GATC ^GATC
357 GATC
HapII 342 CCGG C^CGG
361 CCGG
HhaI 207 GCGC GCG^C
317 GCGC
HinP1I 207 GCGC G^CGC
317 GCGC
HpaII 342 CCGG C^CGG
361 CCGG
HphI 87 GGTGA GGTGA(8/7)
36 TCACC
MaeII 70 ACGT A^CGT
136 ACGT
MboI 222 GATC ^GATC
357 GATC
MboII 21 GAAGA GAAGA(8/7)
387 GAAGA
MslI 42 CATGTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
225 CATCGACATG
MspI 342 CCGG C^CGG
361 CCGG
NdeII 222 GATC ^GATC
357 GATC
NlaIV 344 GGGGCC GG[ACGT]^[ACGT]CC
356 GGATCC
RsaI 72 GTAC GT^AC
201 GTAC
Sau3AI 222 GATC ^GATC
357 GATC
Sau96I 258 GGGCC G^G[ACGT]CC
345 GGGCC
SfaNI 165 GATGC GCATC(5/9)
209 GCATC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AccII AciI BbvI BcnI BsaJI
BsiYI BslI BssKI BstF5I BstNI
BstUI Cac8I DsaI EaeI EcoRII
Fnu4HI FokI HaeIII ItaI KspI
MnlI MspA1I MvaI MvnI MwoI
NciI NlaIII SacII ScrFI TseI