Restriction map for pFN21AB8276
 (Circular) (Six-base) MAPSORT of: pFN21AB8276.seq  Check: 2130  from: 1  to: 5122



pFN21AB8276  5122 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:41  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2932     278 
   Size:         53      83     186     936    1396    2468

  Fragments arranged by size: 

                2468    1396     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2465    1937 
   Size:        528    4594
 
Acc65I G'GTAC_C

Cuts at:   2443    2443 
   Size:       5122
 
AclI AA'CG_TT

Cuts at:   1563    3249    3622    1563 
   Size:       1686     373    3063

  Fragments arranged by size: 

                3063    1686     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2045    3185    4569    4837    1632 
   Size:        413    1140    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1140     413     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4925
 
AflIII A'CryG_T

Cuts at:   1203    4021    4998    1203 
   Size:       2818     977    1327

  Fragments arranged by size: 

                2818    1327     977
 
AgeI A'CCGG_T

Cuts at:   3953    3953 
   Size:       5122
 
AhdI GACnn_n'nnGTC

Cuts at:   3851    3851 
   Size:       5122
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5122
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5122
 
AlwNI CAG_nnn'CTG

Cuts at:   2865    4437    2865 
   Size:       1572    3550
 
ApaLI G'TGCA_C

Cuts at:   3179    4335    3179 
   Size:       1156    3966
 
ApoI r'AATT_y

Cuts at:   1493    2431    2657    2809    4918    1493 
   Size:        938     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     938     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3676     160 
   Size:        872    2644    1606

  Fragments arranged by size: 

                2644    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5122
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2174    2365    2447    1870 
   Size:         73     231     191      82    4545

  Fragments arranged by size: 

                4545     231     191      82      73
 
BamHI G'GATC_C

Cuts at:   2361    2452    2819    2361 
   Size:         91     367    4664

  Fragments arranged by size: 

                4664     367      91
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2443    3898     618 
   Size:        325     217     627     656    1455    1842

  Fragments arranged by size: 

                1842    1455     656     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2441    1391 
   Size:       1050    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2033     928 
   Size:        971     134    4017

  Fragments arranged by size: 

                4017     971     134
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3313    3347    1903 
   Size:         34      50      34    1292      34    3678

  Fragments arranged by size: 

                3678    1292      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3014    4224    3014 
   Size:       1210    3912
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5122
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2277    3733     136 
   Size:        107     122      71     631     605     605    1456    1525

  Fragments arranged by size: 

                1525    1456     631     605     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5117    5117 
   Size:       5122
 
BlpI GC'TnA_GC

Cuts at:   2539    2539 
   Size:       5122
 
Bme1580I G_kGCm'C

Cuts at:   1790    2372    3183    4339    1790 
   Size:        582     811    1156    2573

  Fragments arranged by size: 

                2573    1156     811     582
 
BmgBI CAC'GTC

Cuts at:   1318    2142    1318 
   Size:        824    4298
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2853    3811     442 
   Size:        416      53      83     399      24    1436     958    1753

  Fragments arranged by size: 

                1753    1436     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5122
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3782    1239 
   Size:       2543    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3247    4112    4410    3247 
   Size:        865     298    3959

  Fragments arranged by size: 

                3959     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3785     882 
   Size:        617    2286    2219

  Fragments arranged by size: 

                2286    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2913     493 
   Size:        711    1709    2702

  Fragments arranged by size: 

                2702    1709     711
 
BsaBI GATnn'nnATC

Cuts at:   5116    5116 
   Size:       5122
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2929    3311    3946     275 
   Size:         53      83     186     936    1396     382     635    1451

  Fragments arranged by size: 

                1451    1396     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3555    3953    4227    4374    4785    3555 
   Size:        398     274     147     411    3892

  Fragments arranged by size: 

                3892     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4897

  Fragments arranged by size: 

                4897     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2261    1186 
   Size:       1075    4047
 
BseYI C'CCAG_C

Cuts at:   4325    4821    4325 
   Size:        496    4626
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2122    1163 
   Size:        573     386    4163

  Fragments arranged by size: 

                4163     573     386
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2272    2572    3333    4361    1540 
   Size:        275     186     271     300     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     300     275     271     186
 
BsiHKAI G_wGCw'C

Cuts at:   3183    3268    4339    3183 
   Size:         85    1071    3966

  Fragments arranged by size: 

                3966    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2633    2726    2633 
   Size:         93    5029
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2066    2066 
   Size:       5122
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2372    2441    3183    3268    4339    1391 
   Size:        399     582      69     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     582     399      85      69
 
BspEI T'CCGG_A

Cuts at:   4785    4785 
   Size:       5122
 
BspHI T'CATG_A

Cuts at:   3011    4741    3011 
   Size:       1730    3392
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2130    2217    2477     844 
   Size:       1286      87     260    3489

  Fragments arranged by size: 

                3489    1286     260      87
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3009    1394 
   Size:        429    1186    3507

  Fragments arranged by size: 

                3507    1186     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3617    3791      65 
   Size:       1183    2369     174    1396

  Fragments arranged by size: 

                2369    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3766    3953    1700 
   Size:        110    1956     187    2869

  Fragments arranged by size: 

                2869    1956     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5122
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5122
 
BssSI C'ACGA_G

Cuts at:   3182    4194    3182 
   Size:       1012    4110
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5122
 
BstBI TT'CG_AA

Cuts at:   3926    3926 
   Size:       5122
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5122
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2053    2053 
   Size:       5122
 
BstYI r'GATC_y

Cuts at:   1972    2361    2452    2819    3204    3221    4673    4759    4771 
   Size:        389      91     367     385      17    1452      86      12

Cuts at:   4771    5117    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     389     385     367     346      91      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2188    2266    2272    2308     513 
   Size:        552    1123      78       6      36    3327

  Fragments arranged by size: 

                3327    1123     552      78      36       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4383

  Fragments arranged by size: 

                4383     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2018    2638    3432    3452    2018 
   Size:        620     794      20    3688

  Fragments arranged by size: 

                3688     794     620      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5122
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5087
 
DraI TTT'AAA

Cuts at:   2426    2426 
   Size:       5122
 
DraIII CAC_nnn'GTG

Cuts at:   2092    2092 
   Size:       5122
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4129     809 
   Size:        507    2813    1802

  Fragments arranged by size: 

                2813    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2256    2269    2326 
   Size:         54    1425      50     275      18     426      13      57

Cuts at:   2326    2344    2569    3458    5087       8 
   Size:         18     225     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     426     275     225      57      54
                  50      43      18      18      13
 
EagI C'GGCC_G

Cuts at:   1537    1812    2269    2569    1537 
   Size:        275     457     300    4090

  Fragments arranged by size: 

                4090     457     300     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3052    1353 
   Size:       1699    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2182    3709    4083    4229     387 
   Size:       1064     138     593    1527     374     146    1280

  Fragments arranged by size: 

                1527    1280    1064     593     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2045    3185    3782    4569    4837    1239 
   Size:        393     413    1140     597     787     268    1524

  Fragments arranged by size: 

                1524    1140     787     597     413     393     268
 
EcoRI G'AATT_C

Cuts at:   2431    2809    2431 
   Size:        378    4744
 
FspI TGC'GCA

Cuts at:   2213    3628    2213 
   Size:       1415    3707
 
HaeII r_GCGC'y

Cuts at:   1678    2325    4269    1678 
   Size:        647    1944    2531

  Fragments arranged by size: 

                2531    1944     647
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2118    2150    2346    2378    3763 
   Size:         32     135      32     546      32     196      32    1385

Cuts at:   3763    3795    3837    3869    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1385     546     196     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2466    2712     669 
   Size:        988     281     528     246    3079

  Fragments arranged by size: 

                3079     988     528     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5122
 
HpaI GTT'AAC

Cuts at:   2712    2712 
   Size:       5122
 
KpnI G_GTAC'C

Cuts at:   2447    2447 
   Size:       5122
 
MfeI C'AATT_G

Cuts at:   2721    2721 
   Size:       5122
 
MluI A'CGCG_T

Cuts at:   4998    4998 
   Size:       5122
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4236    4420    1305 
   Size:       2931     184    2007

  Fragments arranged by size: 

                2931    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2258      10 
   Size:         54    1425     343     426    2874

  Fragments arranged by size: 

                2874    1425     426     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2051    2234    3080    3439    3598     518 
   Size:        648     885     183     846     359     159    2042

  Fragments arranged by size: 

                2042     885     846     648     359     183     159
 
MspA1I CmG'CkG

Cuts at:   1606    2190    2268    2274    2310    2538    3215    4363    4608 
   Size:        584      78       6      36     228     677    1148     245

Cuts at:   4608    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     677     584     245     228      78      36
                   6
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4570
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5122
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5122
 
NotI GC'GGCC_GC

Cuts at:   2269    2569    2269 
   Size:        300    4822
 
NspI r_CATG'y

Cuts at:   1132    1229    2480    4025    1132 
   Size:         97    1251    1545    2229

  Fragments arranged by size: 

                2229    1545    1251      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5122
 
PciI A'CATG_T

Cuts at:   4021    4021 
   Size:       5122
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5122
 
PmeI GTTT'AAAC

Cuts at:   2426    2426 
   Size:       5122
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3191    3223    4704    4736    3191 
   Size:         32    1481      32    3577

  Fragments arranged by size: 

                3577    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5122
 
PsiI TTA'TAA

Cuts at:   2692    2692 
   Size:       5122
 
PstI C_TGCA'G

Cuts at:    830    2286    2474     830 
   Size:       1456     188    3478

  Fragments arranged by size: 

                3478    1456     188
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5122
 
SacII CC_GC'GG

Cuts at:   2191    2269    2275    2311    2191 
   Size:         78       6      36    5002

  Fragments arranged by size: 

                5002      78      36       6
 
SalI G'TCGA_C

Cuts at:   1936    2464    1936 
   Size:        528    4594
 
SbfI CC_TGCA'GG

Cuts at:   2474    2474 
   Size:       5122
 
ScaI AGT'ACT

Cuts at:   3370    5082    3370 
   Size:       1712    3410
 
SexAI A'CCwGG_T

Cuts at:   2088    2088 
   Size:       5122
 
SfcI C'TryA_G

Cuts at:    826    1045    2282    2470    3605    4286    4477     826 
   Size:        219    1237     188    1135     681     191    1471

  Fragments arranged by size: 

                1471    1237    1135     681     219     191     188
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2277    2277 
   Size:       5122
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5122
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5122
 
SmaI CCC'GGG

Cuts at:   1872    2367    2449    1872 
   Size:        495      82    4545

  Fragments arranged by size: 

                4545     495      82
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3226    4127    4389     820 
   Size:        197     926    1283     901     262    1553

  Fragments arranged by size: 

                1553    1283     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2913     493 
   Size:       2420    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5122
 
SphI G_CATG'C

Cuts at:   1132    2480    1132 
   Size:       1348    3774
 
SspI AAT'ATT

Cuts at:      5      52    3046       5 
   Size:         47    2994    2081

  Fragments arranged by size: 

                2994    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2280    1517 
   Size:        763    4359
 
StyI C'CwwG_G

Cuts at:    513    1065    2259    2561     513 
   Size:        552    1194     302    3074

  Fragments arranged by size: 

                3074    1194     552     302
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3149    3166    3319    3504    3949     325 
   Size:       1255    1569      17     153     185     445    1498

  Fragments arranged by size: 

                1569    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3368    5080      96 
   Size:        274      80      33      51    1443    1391    1712     138

  Fragments arranged by size: 

                1712    1443    1391     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2076    2108    5082    5114    2076 
   Size:         32    2974      32    2084

  Fragments arranged by size: 

                2974    2084      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2237    1316 
   Size:        921    4201
 
XbaI T'CTAG_A

Cuts at:   2458    2458 
   Size:       5122
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5122
 
XmaI C'CCGG_G

Cuts at:   1870    2365    2447    1870 
   Size:        495      82    4545

  Fragments arranged by size: 

                4545     495      82
 
XmnI GAAnn'nnTTC

Cuts at:   3251    3251 
   Size:       5122
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2930     276 
   Size:         53      83     186     936    1396    2468

  Fragments arranged by size: 

                2468    1396     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    SacII     SalI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI  BstZ17I   Bsu36I  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SanDI     SapI
     SfoI     SrfI     SwaI     XcmI
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