(Circular) (Six-base) MAPSORT of: pFN21AB8276.seq Check: 2130 from: 1 to: 5122 pFN21AB8276 5122 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2932 278 Size: 53 83 186 936 1396 2468 Fragments arranged by size: 2468 1396 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2465 1937 Size: 528 4594 Acc65I G'GTAC_C Cuts at: 2443 2443 Size: 5122 AclI AA'CG_TT Cuts at: 1563 3249 3622 1563 Size: 1686 373 3063 Fragments arranged by size: 3063 1686 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 3185 4569 4837 1632 Size: 413 1140 1384 268 1917 Fragments arranged by size: 1917 1384 1140 413 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4925 AflIII A'CryG_T Cuts at: 1203 4021 4998 1203 Size: 2818 977 1327 Fragments arranged by size: 2818 1327 977 AgeI A'CCGG_T Cuts at: 3953 3953 Size: 5122 AhdI GACnn_n'nnGTC Cuts at: 3851 3851 Size: 5122 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5122 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5122 AlwNI CAG_nnn'CTG Cuts at: 2865 4437 2865 Size: 1572 3550 ApaLI G'TGCA_C Cuts at: 3179 4335 3179 Size: 1156 3966 ApoI r'AATT_y Cuts at: 1493 2431 2657 2809 4918 1493 Size: 938 226 152 2109 1697 Fragments arranged by size: 2109 1697 938 226 152 AseI AT'TA_AT Cuts at: 160 1032 3676 160 Size: 872 2644 1606 Fragments arranged by size: 2644 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5122 AvaI C'yCGr_G Cuts at: 1870 1943 2174 2365 2447 1870 Size: 73 231 191 82 4545 Fragments arranged by size: 4545 231 191 82 73 BamHI G'GATC_C Cuts at: 2361 2452 2819 2361 Size: 91 367 4664 Fragments arranged by size: 4664 367 91 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2443 3898 618 Size: 325 217 627 656 1455 1842 Fragments arranged by size: 1842 1455 656 627 325 217 BanII G_rGCy'C Cuts at: 1391 2441 1391 Size: 1050 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2033 928 Size: 971 134 4017 Fragments arranged by size: 4017 971 134 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3313 3347 1903 Size: 34 50 34 1292 34 3678 Fragments arranged by size: 3678 1292 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3014 4224 3014 Size: 1210 3912 BclI T'GATC_A Cuts at: 1548 1548 Size: 5122 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2277 3733 136 Size: 107 122 71 631 605 605 1456 1525 Fragments arranged by size: 1525 1456 631 605 605 122 107 71 BglII A'GATC_T Cuts at: 5117 5117 Size: 5122 BlpI GC'TnA_GC Cuts at: 2539 2539 Size: 5122 Bme1580I G_kGCm'C Cuts at: 1790 2372 3183 4339 1790 Size: 582 811 1156 2573 Fragments arranged by size: 2573 1156 811 582 BmgBI CAC'GTC Cuts at: 1318 2142 1318 Size: 824 4298 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2853 3811 442 Size: 416 53 83 399 24 1436 958 1753 Fragments arranged by size: 1753 1436 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5122 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3782 1239 Size: 2543 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3247 4112 4410 3247 Size: 865 298 3959 Fragments arranged by size: 3959 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3785 882 Size: 617 2286 2219 Fragments arranged by size: 2286 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2913 493 Size: 711 1709 2702 Fragments arranged by size: 2702 1709 711 BsaBI GATnn'nnATC Cuts at: 5116 5116 Size: 5122 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2929 3311 3946 275 Size: 53 83 186 936 1396 382 635 1451 Fragments arranged by size: 1451 1396 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3555 3953 4227 4374 4785 3555 Size: 398 274 147 411 3892 Fragments arranged by size: 3892 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4897 Fragments arranged by size: 4897 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 1186 Size: 1075 4047 BseYI C'CCAG_C Cuts at: 4325 4821 4325 Size: 496 4626 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2122 1163 Size: 573 386 4163 Fragments arranged by size: 4163 573 386 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2272 2572 3333 4361 1540 Size: 275 186 271 300 761 1028 2301 Fragments arranged by size: 2301 1028 761 300 275 271 186 BsiHKAI G_wGCw'C Cuts at: 3183 3268 4339 3183 Size: 85 1071 3966 Fragments arranged by size: 3966 1071 85 BsmI GAATG_Cn' Cuts at: 2633 2726 2633 Size: 93 5029 BsmBI CGTCTCn'nnnn_ Cuts at: 2066 2066 Size: 5122 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2372 2441 3183 3268 4339 1391 Size: 399 582 69 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 582 399 85 69 BspEI T'CCGG_A Cuts at: 4785 4785 Size: 5122 BspHI T'CATG_A Cuts at: 3011 4741 3011 Size: 1730 3392 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2130 2217 2477 844 Size: 1286 87 260 3489 Fragments arranged by size: 3489 1286 260 87 BsrBI CCG'CTC Cuts at: 1394 1823 3009 1394 Size: 429 1186 3507 Fragments arranged by size: 3507 1186 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3617 3791 65 Size: 1183 2369 174 1396 Fragments arranged by size: 2369 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3766 3953 1700 Size: 110 1956 187 2869 Fragments arranged by size: 2869 1956 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5122 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5122 BssSI C'ACGA_G Cuts at: 3182 4194 3182 Size: 1012 4110 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5122 BstBI TT'CG_AA Cuts at: 3926 3926 Size: 5122 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5122 BstXI CCAn_nnnn'nTGG Cuts at: 2053 2053 Size: 5122 BstYI r'GATC_y Cuts at: 1972 2361 2452 2819 3204 3221 4673 4759 4771 Size: 389 91 367 385 17 1452 86 12 Cuts at: 4771 5117 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 389 385 367 346 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2188 2266 2272 2308 513 Size: 552 1123 78 6 36 3327 Fragments arranged by size: 3327 1123 552 78 36 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4383 Fragments arranged by size: 4383 683 56 BtsI GCAGTG_nn' Cuts at: 2018 2638 3432 3452 2018 Size: 620 794 20 3688 Fragments arranged by size: 3688 794 620 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5122 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5087 DraI TTT'AAA Cuts at: 2426 2426 Size: 5122 DraIII CAC_nnn'GTG Cuts at: 2092 2092 Size: 5122 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4129 809 Size: 507 2813 1802 Fragments arranged by size: 2813 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2256 2269 2326 Size: 54 1425 50 275 18 426 13 57 Cuts at: 2326 2344 2569 3458 5087 8 Size: 18 225 889 1629 43 Fragments arranged by size: 1629 1425 889 426 275 225 57 54 50 43 18 18 13 EagI C'GGCC_G Cuts at: 1537 1812 2269 2569 1537 Size: 275 457 300 4090 Fragments arranged by size: 4090 457 300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3052 1353 Size: 1699 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2182 3709 4083 4229 387 Size: 1064 138 593 1527 374 146 1280 Fragments arranged by size: 1527 1280 1064 593 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 3185 3782 4569 4837 1239 Size: 393 413 1140 597 787 268 1524 Fragments arranged by size: 1524 1140 787 597 413 393 268 EcoRI G'AATT_C Cuts at: 2431 2809 2431 Size: 378 4744 FspI TGC'GCA Cuts at: 2213 3628 2213 Size: 1415 3707 HaeII r_GCGC'y Cuts at: 1678 2325 4269 1678 Size: 647 1944 2531 Fragments arranged by size: 2531 1944 647 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2118 2150 2346 2378 3763 Size: 32 135 32 546 32 196 32 1385 Cuts at: 3763 3795 3837 3869 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1385 546 196 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2466 2712 669 Size: 988 281 528 246 3079 Fragments arranged by size: 3079 988 528 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5122 HpaI GTT'AAC Cuts at: 2712 2712 Size: 5122 KpnI G_GTAC'C Cuts at: 2447 2447 Size: 5122 MfeI C'AATT_G Cuts at: 2721 2721 Size: 5122 MluI A'CGCG_T Cuts at: 4998 4998 Size: 5122 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4236 4420 1305 Size: 2931 184 2007 Fragments arranged by size: 2931 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2258 10 Size: 54 1425 343 426 2874 Fragments arranged by size: 2874 1425 426 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2051 2234 3080 3439 3598 518 Size: 648 885 183 846 359 159 2042 Fragments arranged by size: 2042 885 846 648 359 183 159 MspA1I CmG'CkG Cuts at: 1606 2190 2268 2274 2310 2538 3215 4363 4608 Size: 584 78 6 36 228 677 1148 245 Cuts at: 4608 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 584 245 228 78 36 6 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4570 NdeI CA'TA_TG Cuts at: 387 387 Size: 5122 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5122 NotI GC'GGCC_GC Cuts at: 2269 2569 2269 Size: 300 4822 NspI r_CATG'y Cuts at: 1132 1229 2480 4025 1132 Size: 97 1251 1545 2229 Fragments arranged by size: 2229 1545 1251 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5122 PciI A'CATG_T Cuts at: 4021 4021 Size: 5122 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5122 PmeI GTTT'AAAC Cuts at: 2426 2426 Size: 5122 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3191 3223 4704 4736 3191 Size: 32 1481 32 3577 Fragments arranged by size: 3577 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5122 PsiI TTA'TAA Cuts at: 2692 2692 Size: 5122 PstI C_TGCA'G Cuts at: 830 2286 2474 830 Size: 1456 188 3478 Fragments arranged by size: 3478 1456 188 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5122 SacII CC_GC'GG Cuts at: 2191 2269 2275 2311 2191 Size: 78 6 36 5002 Fragments arranged by size: 5002 78 36 6 SalI G'TCGA_C Cuts at: 1936 2464 1936 Size: 528 4594 SbfI CC_TGCA'GG Cuts at: 2474 2474 Size: 5122 ScaI AGT'ACT Cuts at: 3370 5082 3370 Size: 1712 3410 SexAI A'CCwGG_T Cuts at: 2088 2088 Size: 5122 SfcI C'TryA_G Cuts at: 826 1045 2282 2470 3605 4286 4477 826 Size: 219 1237 188 1135 681 191 1471 Fragments arranged by size: 1471 1237 1135 681 219 191 188 SfiI GGCCn_nnn'nGGCC Cuts at: 2277 2277 Size: 5122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5122 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5122 SmaI CCC'GGG Cuts at: 1872 2367 2449 1872 Size: 495 82 4545 Fragments arranged by size: 4545 495 82 SmlI C'TyrA_G Cuts at: 820 1017 1943 3226 4127 4389 820 Size: 197 926 1283 901 262 1553 Fragments arranged by size: 1553 1283 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2913 493 Size: 2420 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5122 SphI G_CATG'C Cuts at: 1132 2480 1132 Size: 1348 3774 SspI AAT'ATT Cuts at: 5 52 3046 5 Size: 47 2994 2081 Fragments arranged by size: 2994 2081 47 StuI AGG'CCT Cuts at: 1517 2280 1517 Size: 763 4359 StyI C'CwwG_G Cuts at: 513 1065 2259 2561 513 Size: 552 1194 302 3074 Fragments arranged by size: 3074 1194 552 302 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3149 3166 3319 3504 3949 325 Size: 1255 1569 17 153 185 445 1498 Fragments arranged by size: 1569 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3368 5080 96 Size: 274 80 33 51 1443 1391 1712 138 Fragments arranged by size: 1712 1443 1391 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2076 2108 5082 5114 2076 Size: 32 2974 32 2084 Fragments arranged by size: 2974 2084 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2237 1316 Size: 921 4201 XbaI T'CTAG_A Cuts at: 2458 2458 Size: 5122 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5122 XmaI C'CCGG_G Cuts at: 1870 2365 2447 1870 Size: 495 82 4545 Fragments arranged by size: 4545 495 82 XmnI GAAnn'nnTTC Cuts at: 3251 3251 Size: 5122 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2930 276 Size: 53 83 186 936 1396 2468 Fragments arranged by size: 2468 1396 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfoI SrfI SwaI XcmI