Restriction map for
pFN21AB8271
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BalI BamHI BbrPI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DpnII
DraI DraIII DrdI Eam1105I EarI
Eco81I EcoRI EcoRV EcoT22I FbaI
HincII HindII HindIII HinfI HpaI
KpnI Ksp632I MaeI MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NdeII NheI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
Sau3AI ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI TfiI Tru9I Tsp509I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcyI 559 GGCGCC G[GA]^CG[CT]C
AflIII 383 ACGTGT A^C[GA][CT]GT
Aor51HI 125 AGCGCT AGC^GCT
AspHI 438 GAGCAC G[AT]GC[AT]^C
AspI 177 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 88 CCCGAG C^[CT]CG[GA]G
AvaII 725 GGACC G^G[AT]CC
AviII 476 TGCGCA TGC^GCA
BbeI 559 GGCGCC GGCGC^C
BbsI 862 GAAGAC GAAGAC(2/6)
BbvCI 573 CCTCAGC CCTCAGC(-5/-2)
BglI 820 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 207 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 207 GCTCAGC GC^T[ACGT]AGC
BpuAI 862 GAAGAC GAAGAC(2/6)
BsaHI 559 GGCGCC G[GA]^CG[CT]C
BsaI 250 GAGACC GGTCTC(1/5)
BsiHKAI 438 GAGCAC G[AT]GC[AT]^C
BsmBI 316 GAGACG CGTCTC(1/5)
BsoBI 88 CCCGAG C^[CT]CG[GA]G
BsrFI 649 GCCGGC [GA]^CCGG[CT]
BstAPI 420 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 219 GGTCACC G^GT[ACGT]ACC
BstPI 219 GGTCACC G^GT[ACGT]ACC
CelII 207 GCTCAGC GC^T[ACGT]AGC
Cfr10I 649 GCCGGC [GA]^CCGG[CT]
EagI 390 CGGCCG C^GGCCG
EclXI 390 CGGCCG C^GGCCG
Eco47III 125 AGCGCT AGC^GCT
Eco52I 390 CGGCCG C^GGCCG
EcoNI 738 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 219 GGTCACC G^GT[ACGT]ACC
FseI 648 GGCCGGCC GGCCGG^CC
FspI 476 TGCGCA TGC^GCA
HgaI 318 GACGC GACGC(5/10)
Hin1I 559 GGCGCC G[GA]^CG[CT]C
KasI 559 GGCGCC G^GCGCC
KspI 408 CCGCGG CCGC^GG
MaeII 383 ACGT A^CGT
MboII 862 GAAGA GAAGA(8/7)
MspA1I 408 CCGCGG C[AC]G^C[GT]G
NaeI 649 GCCGGC GCC^GGC
NarI 559 GGCGCC GG^CGCC
NgoMIV 649 GCCGGC G^CCGGC
NotI 389 GCGGCCGC GC^GGCCGC
NspI 866 ACATGC [GA]CATG^[CT]
PflFI 177 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 408 CCGCGG CCGC^GG
SbfI 807 CCTGCAGG CCTGCA^GG
SfiI 819 GGCCTTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 559 GGCGCC GGC^GCC
SmlI 835 CTCAAG C^T[CT][GA]AG
Sse8387I 807 CCTGCAGG CCTGCA^GG
TaqI 848 TCGA T^CGA
Tth111I 177 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 848 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AfaI 405 GTAC GT^AC
537 GTAC
AlwNI 175 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
226 CAGGAACTG
BanI 559 GGCGCC G^G[CT][GA]CC
916 GGCACC
BpmI 286 CTGGAG CTGGAG(16/14)
781 CTGGAG
BsiEI 390 CGGCCG CG[GA][CT]^CG
732 CGACCG
DsaI 408 CCGCGG C^C[GA][CT]GG
911 CCGTGG
EcoT14I 53 CCAAGG C^C[AT][AT]GG
335 CCAAGG
HphI 747 GGTGA GGTGA(8/7)
221 TCACC
MaeIII 28 GTCAC ^GT[ACGT]AC
220 GTCAC
RsaI 405 GTAC GT^AC
537 GTAC
SfaNI 44 GATGC GCATC(5/9)
505 GATGC
StyI 53 CCAAGG C^C[AT][AT]GG
335 CCAAGG
Tsp45I 28 GTCAC ^GT[GC]AC
220 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AluI ApaI BanII
BbvI BcnI BmyI BsaJI BseRI
BsgI BsiYI BslI BsmAI Bsp1286I
BspMI BsrBI BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PstI
Sau96I ScrFI SfcI StuI TseI
TspRI