Restriction map for pFN21AB8271
 (Linear) (Six-base) MAPSORT of: KIBB8271.seq  Check: 3391  from: 1  to: 951



KIBB8271.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     180     482     747     951 
   Size:        180     302     265     204

  Fragments arranged by size: 

                 302     265     204     180
 
AfeI AGC'GCT

Cuts at:      0     127     951 
   Size:        127     824
 
AflIII A'CryG_T

Cuts at:      0     383     951 
   Size:        383     568
 
AlwNI CAG_nnn'CTG

Cuts at:      0     180     231     951 
   Size:        180      51     720

  Fragments arranged by size: 

                 720     180      51
 
ApaI G_GGCC'C

Cuts at:      0     552     602     675     951 
   Size:        552      50      73     276

  Fragments arranged by size: 

                 552     276      73      50
 
AvaI C'yCGr_G

Cuts at:      0      88     951 
   Size:         88     863
 
BanI G'GyrC_C

Cuts at:      0     559     916     951 
   Size:        559     357      35

  Fragments arranged by size: 

                 559     357      35
 
BanII G_rGCy'C

Cuts at:      0      89     552     602     643     675     854     951 
   Size:         89     463      50      41      32     179      97

  Fragments arranged by size: 

                 463     179      97      89      50      41      32
 
BbeI G_GCGC'C

Cuts at:      0     563     951 
   Size:        563     388
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     869     951 
   Size:        869      82
 
BbvCI CC'TCA_GC

Cuts at:      0     574     951 
   Size:        574     377
 
BglI GCCn_nnn'nGGC

Cuts at:      0     826     951 
   Size:        826     125
 
BlpI GC'TnA_GC

Cuts at:      0     208     951 
   Size:        208     743
 
Bme1580I G_kGCm'C

Cuts at:      0     327     552     602     675     919     938     951 
   Size:        327     225      50      73     244      19      13

  Fragments arranged by size: 

                 327     244     225      73      50      19      13
 
BmgBI CAC'GTC

Cuts at:      0     384     951 
   Size:        384     567
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     151     951 
   Size:        151     800
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     802     951 
   Size:        307     495     149

  Fragments arranged by size: 

                 495     307     149
 
Bpu10I CC'TnA_GC

Cuts at:      0     574     951 
   Size:        574     377
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820     951 
   Size:        820     131
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     244     951 
   Size:        244     707
 
BsaHI Gr'CG_yC

Cuts at:      0     560     951 
   Size:        560     391
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     370     400     951 
   Size:        370      30     551

  Fragments arranged by size: 

                 551     370      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     214     328     460     487     724     799     951 
   Size:        214     114     132      27     237      75     152

  Fragments arranged by size: 

                 237     214     152     132     114      75      27
 
BseYI C'CCAG_C

Cuts at:      0     128     167     363     794     937     951 
   Size:        128      39     196     431     143      14

  Fragments arranged by size: 

                 431     196     143     128      39      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     214     421     439     922     951 
   Size:        193      21     207      18     483      29

  Fragments arranged by size: 

                 483     207     193      29      21      18
 
BsiEI CG_ry'CG

Cuts at:      0     393     735     951 
   Size:        393     342     216

  Fragments arranged by size: 

                 393     342     216
 
BsiHKAI G_wGCw'C

Cuts at:      0     442     951 
   Size:        442     509
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     310     951 
   Size:        310     641
 
Bsp1286I G_dGCh'C

Cuts at:      0      89     327     442     552     602     643     675     854 
   Size:         89     238     115     110      50      41      32     179

Cuts at:    854     919     938     951 
   Size:         65      19      13

  Fragments arranged by size: 

                 238     179     115     110      89      65      50      41
                  32      19      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     170     180     482     524     747     951 
   Size:        170      10     302      42     223     204

  Fragments arranged by size: 

                 302     223     204     170      42      10
 
BsrBI CCG'CTC

Cuts at:      0     119     669     690     951 
   Size:        119     550      21     261

  Fragments arranged by size: 

                 550     261     119      21
 
BsrFI r'CCGG_y

Cuts at:      0     649     951 
   Size:        649     302
 
BssHII G'CGCG_C

Cuts at:      0     696     698     771     951 
   Size:        696       2      73     180

  Fragments arranged by size: 

                 696     180      73       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     426     951 
   Size:        426     525
 
BstEII G'GTnAC_C

Cuts at:      0     219     951 
   Size:        219     732
 
BtgI C'CryG_G

Cuts at:      0     408     911     951 
   Size:        408     503      40

  Fragments arranged by size: 

                 503     408      40
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     518     951 
   Size:        518     433
 
BtsI GCAGTG_nn'

Cuts at:      0     539     951 
   Size:        539     412
 
EaeI y'GGCC_r

Cuts at:      0     120     390     432     647     651     951 
   Size:        120     270      42     215       4     300

  Fragments arranged by size: 

                 300     270     215     120      42       4
 
EagI C'GGCC_G

Cuts at:      0     390     951 
   Size:        390     561
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     325     778     951 
   Size:        325     453     173

  Fragments arranged by size: 

                 453     325     173
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     802     951 
   Size:        307     495     149

  Fragments arranged by size: 

                 495     307     149
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     742     951 
   Size:        742     209
 
EcoO109I rG'GnC_Cy

Cuts at:      0     548     598     599     951 
   Size:        548      50       1     352

  Fragments arranged by size: 

                 548     352      50       1
 
FseI GG_CCGG'CC

Cuts at:      0     653     951 
   Size:        653     298
 
FspI TGC'GCA

Cuts at:      0     478     951 
   Size:        478     473
 
HaeII r_GCGC'y

Cuts at:      0     129     241     528     563     581     594     876     926 
   Size:        129     112     287      35      18      13     282      50

Cuts at:    926     951 
   Size:         25

  Fragments arranged by size: 

                 287     282     129     112      50      35      25      18
                  13
 
KasI G'GCGC_C

Cuts at:      0     559     951 
   Size:        559     392
 
MspA1I CmG'CkG

Cuts at:      0     410     951 
   Size:        410     541
 
NaeI GCC'GGC

Cuts at:      0     651     951 
   Size:        651     300
 
NarI GG'CG_CC

Cuts at:      0     560     951 
   Size:        560     391
 
NgoMIV G'CCGG_C

Cuts at:      0     649     951 
   Size:        649     302
 
NotI GC'GGCC_GC

Cuts at:      0     390     951 
   Size:        390     561
 
NspI r_CATG'y

Cuts at:      0     870     951 
   Size:        870      81
 
PspOMI G'GGCC_C

Cuts at:      0     548     598     671     951 
   Size:        548      50      73     280

  Fragments arranged by size: 

                 548     280      73      50
 
PstI C_TGCA'G

Cuts at:      0     347     521     812     951 
   Size:        347     174     291     139

  Fragments arranged by size: 

                 347     291     174     139
 
SacII CC_GC'GG

Cuts at:      0     411     951 
   Size:        411     540
 
SbfI CC_TGCA'GG

Cuts at:      0     812     951 
   Size:        812     139
 
SfcI C'TryA_G

Cuts at:      0     343     517     661     808     951 
   Size:        343     174     144     147     143

  Fragments arranged by size: 

                 343     174     147     144     143
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     826     951 
   Size:        826     125
 
SfoI GGC'GCC

Cuts at:      0     561     951 
   Size:        561     390
 
SmlI C'TyrA_G

Cuts at:      0     835     951 
   Size:        835     116
 
StuI AGG'CCT

Cuts at:      0     271     679     820     951 
   Size:        271     408     141     131

  Fragments arranged by size: 

                 408     271     141     131
 
StyI C'CwwG_G

Cuts at:      0      53     335     951 
   Size:         53     282     616

  Fragments arranged by size: 

                 616     282      53
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     121     159     238     951 
   Size:        121      38      79     713

  Fragments arranged by size: 

                 713     121      79      38
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     370     402     951 
   Size:        370      32     549

  Fragments arranged by size: 

                 549     370      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     180     951 
   Size:        180     771
 

 Enzymes that do cut:

     AarI     AfeI   AflIII    AlwNI     ApaI     AvaI     BanI    BanII
     BbeI     BbsI    BbvCI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI
   BssHII   BstAPI   BstEII     BtgI    BtgZI     BtsI     EaeI     EagI
     EciI  Eco57MI    EcoNI EcoO109I     FseI     FspI    HaeII     KasI
   MspA1I     NaeI     NarI   NgoMIV     NotI     NspI   PspOMI     PstI
    SacII     SbfI     SfcI     SfiI     SfoI     SmlI     StuI     StyI
    TaqII     TstI  Tth111I

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI    BglII
     BmtI     BplI    BsaAI    BsaBI    BsaWI    BsiWI     BsmI    BspEI
    BspHI    BsrDI    BsrGI    BssSI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EarI  EcoICRI
    EcoRI    EcoRV     FalI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NcoI     NdeI
     NheI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI     ScaI
    SexAI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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