Restriction map for pFN21AB8265
 (Linear) (Six-base) MAPSORT of: KIBB8265.seq  Check: 7813  from: 1  to: 1353



KIBB8265.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AccI GT'mk_AC

Cuts at:      0     203     611    1353 
   Size:        203     408     742

  Fragments arranged by size: 

                 742     408     203
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     379    1353 
   Size:        141     238     974

  Fragments arranged by size: 

                 974     238     141
 
AgeI A'CCGG_T

Cuts at:      0     388    1353 
   Size:        388     965
 
AleI CACnn'nnGTG

Cuts at:      0    1084    1353 
   Size:       1084     269
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     199    1353 
   Size:        199    1154
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      71     103    1353 
   Size:         71      32    1250

  Fragments arranged by size: 

                1250      71      32
 
ApaLI G'TGCA_C

Cuts at:      0     384    1353 
   Size:        384     969
 
ApoI r'AATT_y

Cuts at:      0     362     760    1353 
   Size:        362     398     593

  Fragments arranged by size: 

                 593     398     362
 
AvaI C'yCGr_G

Cuts at:      0     188     311    1353 
   Size:        188     123    1042

  Fragments arranged by size: 

                1042     188     123
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1212    1245    1353 
   Size:       1212      33     108

  Fragments arranged by size: 

                1212     108      33
 
BanI G'GyrC_C

Cuts at:      0     241     519     777    1037    1086    1353 
   Size:        241     278     258     260      49     267

  Fragments arranged by size: 

                 278     267     260     258     241      49
 
BanII G_rGCy'C

Cuts at:      0      68     551     741    1167    1353 
   Size:         68     483     190     426     186

  Fragments arranged by size: 

                 483     426     190     186      68
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     385    1353 
   Size:        385     968
 
BbvCI CC'TCA_GC

Cuts at:      0    1154    1353 
   Size:       1154     199
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1046    1353 
   Size:       1046     307
 
BlpI GC'TnA_GC

Cuts at:      0    1132    1353 
   Size:       1132     221
 
Bme1580I G_kGCm'C

Cuts at:      0     188     320     388     782    1042    1353 
   Size:        188     132      68     394     260     311

  Fragments arranged by size: 

                 394     311     260     188     132      68
 
BmgBI CAC'GTC

Cuts at:      0     966    1353 
   Size:        966     387
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      30    1353 
   Size:         30    1323
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     604    1353 
   Size:        520      84     749

  Fragments arranged by size: 

                 749     520      84
 
Bpu10I CC'TnA_GC

Cuts at:      0     266    1154    1353 
   Size:        266     888     199

  Fragments arranged by size: 

                 888     266     199
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1303    1353 
   Size:       1303      50
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1091    1353 
   Size:       1091     262
 
BsaAI yAC'GTr

Cuts at:      0     183    1224    1353 
   Size:        183    1041     129

  Fragments arranged by size: 

                1041     183     129
 
BsaWI w'CCGG_w

Cuts at:      0     388    1353 
   Size:        388     965
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     146    1312    1353 
   Size:        146    1166      41

  Fragments arranged by size: 

                1166     146      41
 
BseYI C'CCAG_C

Cuts at:      0      59    1353 
   Size:         59    1294
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     388     590    1353 
   Size:         68     320     202     763

  Fragments arranged by size: 

                 763     320     202      68
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     157     472    1353 
   Size:        157     315     881

  Fragments arranged by size: 

                 881     315     157
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     188     320     388     551     590     741     782 
   Size:         68     120     132      68     163      39     151      41

Cuts at:    782    1042    1167    1353 
   Size:        260     125     186

  Fragments arranged by size: 

                 260     186     163     151     132     125     120      68
                  68      41      39
 
BsrDI GCAATG_nn'

Cuts at:      0      57     992    1148    1353 
   Size:         57     935     156     205

  Fragments arranged by size: 

                 935     205     156      57
 
BsrFI r'CCGG_y

Cuts at:      0     388    1353 
   Size:        388     965
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      56    1353 
   Size:         56    1297
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      97     421     903    1353 
   Size:         97     324     482     450

  Fragments arranged by size: 

                 482     450     324      97
 
BtgI C'CryG_G

Cuts at:      0     924     957    1353 
   Size:        924      33     396

  Fragments arranged by size: 

                 924     396      33
 
BtsI GCAGTG_nn'

Cuts at:      0     713    1353 
   Size:        713     640
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     204     239    1131    1166    1353 
   Size:        204      35     892      35     187

  Fragments arranged by size: 

                 892     204     187      35      35
 
EaeI y'GGCC_r

Cuts at:      0      27      99     806    1103    1353 
   Size:         27      72     707     297     250

  Fragments arranged by size: 

                 707     297     250      72      27
 
EarI CTCTTCn'nnn_

Cuts at:      0     262    1353 
   Size:        262    1091
 
EcoICRI GAG'CTC

Cuts at:      0      66    1353 
   Size:         66    1287
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     379     520     604    1353 
   Size:        141     238     141      84     749

  Fragments arranged by size: 

                 749     238     141     141      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1105    1285    1353 
   Size:       1105     180      68

  Fragments arranged by size: 

                1105     180      68
 
EcoRI G'AATT_C

Cuts at:      0     362     760    1353 
   Size:        362     398     593

  Fragments arranged by size: 

                 593     398     362
 
EcoRV GAT'ATC

Cuts at:      0     654    1353 
   Size:        654     699
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1224    1353 
   Size:       1224     129
 
HaeII r_GCGC'y

Cuts at:      0     173     663    1353 
   Size:        173     490     690

  Fragments arranged by size: 

                 690     490     173
 
HincII GTy'rAC

Cuts at:      0     750    1353 
   Size:        750     603
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     335    1353 
   Size:        335    1018
 
MscI TGG'CCA

Cuts at:      0      29     101     808    1105    1353 
   Size:         29      72     707     297     248

  Fragments arranged by size: 

                 707     297     248      72      29
 
MslI CAynn'nnrTG

Cuts at:      0    1084    1353 
   Size:       1084     269
 
MspA1I CmG'CkG

Cuts at:      0     538    1353 
   Size:        538     815
 
NcoI C'CATG_G

Cuts at:      0     924    1353 
   Size:        924     429
 
NdeI CA'TA_TG

Cuts at:      0     813    1353 
   Size:        813     540
 
NspI r_CATG'y

Cuts at:      0    1131    1353 
   Size:       1131     222
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1029    1353 
   Size:       1029     324
 
PmlI CAC'GTG

Cuts at:      0     183    1353 
   Size:        183    1170
 
PshAI GACnn'nnGTC

Cuts at:      0     635    1353 
   Size:        635     718
 
PvuII CAG'CTG

Cuts at:      0     538    1353 
   Size:        538     815
 
SacI G_AGCT'C

Cuts at:      0      68    1353 
   Size:         68    1285
 
SapI GCTCTTCn'nnn_

Cuts at:      0     262    1353 
   Size:        262    1091
 
SexAI A'CCwGG_T

Cuts at:      0     773    1353 
   Size:        773     580
 
SfcI C'TryA_G

Cuts at:      0     539     860    1353 
   Size:        539     321     493

  Fragments arranged by size: 

                 539     493     321
 
SmaI CCC'GGG

Cuts at:      0     190    1353 
   Size:        190    1163
 
SmlI C'TyrA_G

Cuts at:      0    1282    1353 
   Size:       1282      71
 
SphI G_CATG'C

Cuts at:      0    1131    1353 
   Size:       1131     222
 
StuI AGG'CCT

Cuts at:      0    1159    1353 
   Size:       1159     194
 
StyI C'CwwG_G

Cuts at:      0     924    1106    1353 
   Size:        924     182     247

  Fragments arranged by size: 

                 924     247     182
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     374     586    1216    1353 
   Size:        374     212     630     137

  Fragments arranged by size: 

                 630     374     212     137
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     421    1353 
   Size:        421     932
 
XmaI C'CCGG_G

Cuts at:      0     188    1353 
   Size:        188    1165
 
XmnI GAAnn'nnTTC

Cuts at:      0     435    1353 
   Size:        435     918
 

 Enzymes that do cut:

     AccI     AcuI     AgeI     AleI     AlfI     AloI    ApaLI     ApoI
     AvaI     BaeI     BanI    BanII     BbsI    BbvCI     BglI     BlpI
 Bme1580I    BmgBI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaWI    BseRI    BseYI  BsiHKAI    BsmBI Bsp1286I    BsrDI    BsrFI
   BstAPI    BstXI     BtgI     BtsI    CspCI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI    HaeII   HincII     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NspI    PflMI     PmlI
    PshAI    PvuII     SacI     SapI    SexAI     SfcI     SmaI     SmlI
     SphI     StuI     StyI    TaqII     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
    AlwNI     ApaI     AscI     AseI    AsiSI    AvrII    BamHI     BbeI
     BcgI    BciVI     BclI    BfrBI    BglII     BmrI     BmtI    BsaBI
    BsaHI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrBI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI     EagI
     EciI EcoO109I     FseI     FspI    FspAI    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     ZraI
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