Restriction map for
pFN21AB8264
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HindIII HinfI
HpaI KasI KpnI Ksp632I MamI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 355 GTCGAC GT^[AC][GT]AC
BamHI 515 GGATCC G^GATCC
BfaI 430 CTAG C^TAG
BspHI 449 TCATGA T^CATGA
BsrI 587 CCAGT ACTGG(1/-1)
BssHII 538 GCGCGC G^CGCGC
BssSI 424 CACGAG CACGAG(-5/-1)
BstEII 81 GGTGACC G^GT[ACGT]ACC
BstPI 81 GGTGACC G^GT[ACGT]ACC
BstXI 63 CCACGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 515 GGATCC [GA]^GATC[CT]
DdeI 437 CTGAG C^T[ACGT]AG
DraII 527 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 154 CGGCCG [CT]^GGCC[GA]
EagI 154 CGGCCG C^GGCCG
EclXI 154 CGGCCG C^GGCCG
Eco52I 154 CGGCCG C^GGCCG
EcoO109I 527 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 81 GGTGACC G^GT[ACGT]ACC
FauI 523 GCGGG CCCGC(4/6)
HincII 355 GTCGAC GT[CT]^[GA]AC
HindII 355 GTCGAC GT[CT]^[GA]AC
KspI 157 CCGCGG CCGC^GG
MaeI 430 CTAG C^TAG
MboII 573 GAAGA GAAGA(8/7)
MflI 515 GGATCC [GA]^GATC[CT]
NotI 153 GCGGCCGC GC^GGCCGC
NspI 278 GCATGC [GA]CATG^[CT]
PflMI 102 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 527 GGGACCC [GA]G^G[AT]CC[CT]
PstI 391 CTGCAG CTGCA^G
RcaI 449 TCATGA T^CATGA
SacII 157 CCGCGG CCGC^GG
SalI 355 GTCGAC G^TCGAC
SfaNI 248 GCATC GCATC(5/9)
SfcI 391 CTGCAG C^T[GA][CT]AG
SphI 278 GCATGC GCATG^C
TaqI 356 TCGA T^CGA
Tsp509I 128 AATT ^AATT
TspRI 588 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 356 TCGA T^CGA
Van91I 102 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 515 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 158 CGCG CG^CG
539 CGCG
AlwI 516 GATCC GGATC(4/5)
515 GGATC
AspHI 421 GAGCAC G[AT]GC[AT]^C
439 GAGCAC
AvaII 168 GGACC G^G[AT]CC
528 GGACC
BanII 235 GAGCCC G[GA]GC[CT]^C
302 GGGCTC
BpmI 109 CTGGAG CTGGAG(16/14)
379 CTGGAG
BsgI 316 GTGCAG GTGCAG(16/14)
625 GTGCAG
BsiEI 66 CGACCG CG[GA][CT]^CG
154 CGGCCG
BsiHKAI 421 GAGCAC G[AT]GC[AT]^C
439 GAGCAC
BsmAI 16 GAGAC GTCTC(1/5)
592 GAGAC
BsmBI 16 GAGACG CGTCTC(1/5)
592 GAGACG
BspMI 564 GCAGGT ACCTGC(4/8)
602 GCAGGT
BsrFI 23 ACCGGC [GA]^CCGG[CT]
617 ACCGGC
BstAPI 384 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
396 GCAGGAGCTGC
BstF5I 375 CATCC GGATG(2/0)
579 CATCC
BstUI 158 CGCG CG^CG
539 CGCG
Cfr10I 23 ACCGGC [GA]^CCGG[CT]
617 ACCGGC
DpnII 516 GATC ^GATC
576 GATC
DsaI 69 CCGTGG C^C[GA][CT]GG
157 CCGCGG
EcoT14I 239 CCAAGG C^C[AT][AT]GG
455 CCAAGG
FokI 375 CATCC GGATG(9/13)
579 CATCC
MaeII 19 ACGT A^CGT
443 ACGT
MaeIII 82 GTGAC ^GT[ACGT]AC
466 GTGAC
MboI 516 GATC ^GATC
576 GATC
MvnI 158 CGCG CG^CG
539 CGCG
NdeII 516 GATC ^GATC
576 GATC
PvuII 55 CAGCTG CAG^CTG
505 CAGCTG
Sau3AI 516 GATC ^GATC
576 GATC
StyI 239 CCAAGG C^C[AT][AT]GG
455 CCAAGG
Tsp45I 82 GTGAC ^GT[GC]AC
466 GTGAC
XcmI 341 CCAAGCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
368 CCAAGTACATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI AluI AlwNI BbvI
BmyI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MnlI MslI MspA1I MspI
MvaI MwoI NlaIII NlaIV RsaI
Sau96I ScrFI TseI