Restriction map for pFN21AB8258
 (Circular) (Six-base) MAPSORT of: pFN21AB8258.seq  Check: 1046  from: 1  to: 5422



pFN21AB8258  5422 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:41  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2425    2425 
   Size:       5422
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3232     278 
   Size:         53      83     186     936    1696    2468

  Fragments arranged by size: 

                2468    1696     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2765    1937 
   Size:        828    4594
 
Acc65I G'GTAC_C

Cuts at:   2743    2743 
   Size:       5422
 
AclI AA'CG_TT

Cuts at:   1563    3549    3922    1563 
   Size:       1986     373    3063

  Fragments arranged by size: 

                3063    1986     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3485    4869    5137    1632 
   Size:       1853    1384     268    1917

  Fragments arranged by size: 

                1917    1853    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5225
 
AflIII A'CryG_T

Cuts at:   1203    4321    5298    1203 
   Size:       3118     977    1327

  Fragments arranged by size: 

                3118    1327     977
 
AgeI A'CCGG_T

Cuts at:   4253    4253 
   Size:       5422
 
AhdI GACnn_n'nnGTC

Cuts at:   4151    4151 
   Size:       5422
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5422
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2496    2589    1184 
   Size:       1312      93    4017

  Fragments arranged by size: 

                4017    1312      93
 
AlwNI CAG_nnn'CTG

Cuts at:   2255    2273    3165    4737    2255 
   Size:         18     892    1572    2940

  Fragments arranged by size: 

                2940    1572     892      18
 
ApaLI G'TGCA_C

Cuts at:   3479    4635    3479 
   Size:       1156    4266
 
ApoI r'AATT_y

Cuts at:   1493    2693    2731    2957    3109    5218    1493 
   Size:       1200      38     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1200     226     152      38
 
AseI AT'TA_AT

Cuts at:    160    1032    3976     160 
   Size:        872    2944    1606

  Fragments arranged by size: 

                2944    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5422
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2028    2747    1870 
   Size:         73      85     719    4545

  Fragments arranged by size: 

                4545     719      85      73
 
BamHI G'GATC_C

Cuts at:   2752    3119    2752 
   Size:        367    5055
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2743    4198     618 
   Size:        325     217     627     956    1455    1842

  Fragments arranged by size: 

                1842    1455     956     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2504    2741    1391 
   Size:       1113     237    4072

  Fragments arranged by size: 

                4072    1113     237
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2388     928 
   Size:        971     489    3962

  Fragments arranged by size: 

                3962     971     489
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3613    3647    1903 
   Size:         34    1676      34    3678

  Fragments arranged by size: 

                3678    1676      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3314    4524    3314 
   Size:       1210    4212
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5422
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    4033     136 
   Size:        107     122      71     631     605     337    2024    1525

  Fragments arranged by size: 

                2024    1525     631     605     337     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5417    5417 
   Size:       5422
 
BlpI GC'TnA_GC

Cuts at:   2248    2839    2248 
   Size:        591    4831
 
Bme1580I G_kGCm'C

Cuts at:   1790    3483    4639    1790 
   Size:       1693    1156    2573

  Fragments arranged by size: 

                2573    1693    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5422
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2089    2112    2181 
   Size:        416      53      83     399      24     672      23      69

Cuts at:   2181    3153    4111     442 
   Size:        972     958    1753

  Fragments arranged by size: 

                1753     972     958     672     416     399      83      69
                  53      24      23
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5422
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2058    2143    2596    4082    1239 
   Size:        819      85     453    1486    2579

  Fragments arranged by size: 

                2579    1486     819     453      85
 
Bpu10I CC'TnA_GC

Cuts at:   2242    2242 
   Size:       5422
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2406    3547    4412    4710    2406 
   Size:       1141     865     298    3118

  Fragments arranged by size: 

                3118    1141     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2673    4085     882 
   Size:        617    1174    1412    2219

  Fragments arranged by size: 

                2219    1412    1174     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3213     493 
   Size:        711    2009    2702

  Fragments arranged by size: 

                2702    2009     711
 
BsaBI GATnn'nnATC

Cuts at:   5416    5416 
   Size:       5422
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3229    3611    4246     275 
   Size:         53      83     186     936    1696     382     635    1451

  Fragments arranged by size: 

                1696    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3855    4253    4527    4674    5085    3855 
   Size:        398     274     147     411    4192

  Fragments arranged by size: 

                4192     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2421    2451    1178 
   Size:         30     165      30    1018      30    4149

  Fragments arranged by size: 

                4149    1018     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2634    1186 
   Size:       1448    3974
 
BseYI C'CCAG_C

Cuts at:   2412    4625    5121    2412 
   Size:       2213     496    2713

  Fragments arranged by size: 

                2713    2213     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2144    1163 
   Size:        573     408    4441

  Fragments arranged by size: 

                4441     573     408
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2224    2872    3633    4661    1540 
   Size:        275     186     223     648     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     648     275     223     186
 
BsiHKAI G_wGCw'C

Cuts at:   2504    3483    3568    4639    2504 
   Size:        979      85    1071    3287

  Fragments arranged by size: 

                3287    1071     979      85
 
BsmI GAATG_Cn'

Cuts at:   2933    3026    2933 
   Size:         93    5329
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2504    2741    3483    3568    4639    1391 
   Size:        399     714     237     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     714     399     237      85
 
BspEI T'CCGG_A

Cuts at:   5085    5085 
   Size:       5422
 
BspHI T'CATG_A

Cuts at:   3311    5041    3311 
   Size:       1730    3692
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2425    2777     844 
   Size:       1581     352    3489

  Fragments arranged by size: 

                3489    1581     352
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2602    2662    3309    1394 
   Size:        429     779      60     647    3507

  Fragments arranged by size: 

                3507     779     647     429      60
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2640    3917    4091      65 
   Size:       1183    1392    1277     174    1396

  Fragments arranged by size: 

                1396    1392    1277    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2047    4066    4253    1700 
   Size:        110     237    2019     187    2869

  Fragments arranged by size: 

                2869    2019     237     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5422
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5422
 
BssSI C'ACGA_G

Cuts at:   3482    4494    3482 
   Size:       1012    4410
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2232    2642     794 
   Size:       1438     410    3574

  Fragments arranged by size: 

                3574    1438     410
 
BstBI TT'CG_AA

Cuts at:   4226    4226 
   Size:       5422
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5422
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2116    2116 
   Size:       5422
 
BstYI r'GATC_y

Cuts at:   1972    2752    3119    3504    3521    4973    5059    5071    5417 
   Size:        780     367     385      17    1452      86      12     346

Cuts at:   5417    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     780     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2595     513 
   Size:        552     939     591    3340

  Fragments arranged by size: 

                3340     939     591     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2167    2389     487 
   Size:        683      56     941     222    3520

  Fragments arranged by size: 

                3520     941     683     222      56
 
BtsI GCAGTG_nn'

Cuts at:   2571    2938    3732    3752    2571 
   Size:        367     794      20    4241

  Fragments arranged by size: 

                4241     794     367      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5422
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5387
 
DraI TTT'AAA

Cuts at:   2726    2726 
   Size:       5422
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4429     809 
   Size:        507    3113    1802

  Fragments arranged by size: 

                3113    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2118    2592    2869 
   Size:         54    1425      50     275      18     288     474     277

Cuts at:   2869    3758    5387       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     474     288     277     275      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2869    1537 
   Size:        275    1057    4090

  Fragments arranged by size: 

                4090    1057     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2550    3352    1353 
   Size:       1197     802    3423

  Fragments arranged by size: 

                3423    1197     802
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2168    2618    4009    4383    4529     387 
   Size:       1064     138     579     450    1391     374     146    1280

  Fragments arranged by size: 

                1391    1280    1064     579     450     374     146     138
            
 
EcoICRI GAG'CTC

Cuts at:   2502    2502 
   Size:       5422
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2058    2143    2596    3485    4082    4869    5137 
   Size:        393     426      85     453     889     597     787     268

Cuts at:   5137    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     889     787     597     453     426     393     268
                  85
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2354    2424    2354 
   Size:         70    5352
 
EcoO109I rG'GnC_Cy

Cuts at:   2175    2416    2175 
   Size:        241    5181
 
EcoRI G'AATT_C

Cuts at:   2731    3109    2731 
   Size:        378    5044
 
FspI TGC'GCA

Cuts at:   3928    3928 
   Size:       5422
 
HaeII r_GCGC'y

Cuts at:   1678    4569    1678 
   Size:       2891    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2610    2642    4063    4095    4137 
   Size:         32     135      32    1038      32    1421      32      42

Cuts at:   4137    4169    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1421    1038     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2766    3012     669 
   Size:        988     281     828     246    3079

  Fragments arranged by size: 

                3079     988     828     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5422
 
HpaI GTT'AAC

Cuts at:   3012    3012 
   Size:       5422
 
KpnI G_GTAC'C

Cuts at:   2747    2747 
   Size:       5422
 
MfeI C'AATT_G

Cuts at:   3021    3021 
   Size:       5422
 
MluI A'CGCG_T

Cuts at:   5298    5298 
   Size:       5422
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4536    4720    1305 
   Size:       3231     184    2007

  Fragments arranged by size: 

                3231    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2594      10 
   Size:         54    1425     343     762    2838

  Fragments arranged by size: 

                2838    1425     762     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2114    2120    3380    3739    3898     518 
   Size:        648     948       6    1260     359     159    2042

  Fragments arranged by size: 

                2042    1260     948     648     359     159       6
 
MspA1I CmG'CkG

Cuts at:   1606    2252    2838    3515    4663    4908    1606 
   Size:        646     586     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     646     586     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2595     513 
   Size:        552     939     591    3340

  Fragments arranged by size: 

                3340     939     591     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5422
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5422
 
NotI GC'GGCC_GC

Cuts at:   2869    2869 
   Size:       5422
 
NspI r_CATG'y

Cuts at:   1132    1229    2780    4325    1132 
   Size:         97    1551    1545    2229

  Fragments arranged by size: 

                2229    1551    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5422
 
PciI A'CATG_T

Cuts at:   4321    4321 
   Size:       5422
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5422
 
PfoI T'CCnGG_A

Cuts at:   2495    2495 
   Size:       5422
 
PmeI GTTT'AAAC

Cuts at:   2726    2726 
   Size:       5422
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3491    3523    5004    5036    3491 
   Size:         32    1481      32    3877

  Fragments arranged by size: 

                3877    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2175    2416    2175 
   Size:        241    5181
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5422
 
PsiI TTA'TAA

Cuts at:   2992    2992 
   Size:       5422
 
PstI C_TGCA'G

Cuts at:    830    2774     830 
   Size:       1944    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5422
 
PvuII CAG'CTG

Cuts at:   2252    2252 
   Size:       5422
 
SacI G_AGCT'C

Cuts at:   2504    2504 
   Size:       5422
 
SalI G'TCGA_C

Cuts at:   1936    2764    1936 
   Size:        828    4594
 
SanDI GG'GwC_CC

Cuts at:   2416    2416 
   Size:       5422
 
SbfI CC_TGCA'GG

Cuts at:   2774    2774 
   Size:       5422
 
ScaI AGT'ACT

Cuts at:   2670    3670    5382    2670 
   Size:       1000    1712    2710

  Fragments arranged by size: 

                2710    1712    1000
 
SfcI C'TryA_G

Cuts at:    826    1045    2770    3905    4586    4777     826 
   Size:        219    1725    1135     681     191    1471

  Fragments arranged by size: 

                1725    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5422
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5422
 
SmaI CCC'GGG

Cuts at:   1872    2749    1872 
   Size:        877    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2421    3526    4427    4689     820 
   Size:        197     926     478    1105     901     262    1553

  Fragments arranged by size: 

                1553    1105     926     901     478     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3213     493 
   Size:       2720    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5422
 
SphI G_CATG'C

Cuts at:   1132    2780    1132 
   Size:       1648    3774
 
SspI AAT'ATT

Cuts at:      5      52    3346       5 
   Size:         47    3294    2081

  Fragments arranged by size: 

                3294    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5422
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2550    2595    2861     513 
   Size:        552     939     546      45     266    3074

  Fragments arranged by size: 

                3074     939     552     546     266      45
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3449    3466    3619    3804    4249     325 
   Size:       1255    1869      17     153     185     445    1498

  Fragments arranged by size: 

                1869    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2439    2668    3668 
   Size:        274      80      33      51    1443     462     229    1000

Cuts at:   3668    5380      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1000     462     274     229     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2421    2453    5382    5414    2421 
   Size:         32    2929      32    2429

  Fragments arranged by size: 

                2929    2429      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5422
 
XbaI T'CTAG_A

Cuts at:   2758    2758 
   Size:       5422
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2049    2049 
   Size:       5422
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5422
 
XmaI C'CCGG_G

Cuts at:   1870    2747    1870 
   Size:        877    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3551    3551 
   Size:       5422
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3230     276 
   Size:         53      83     186     936    1696    2468

  Fragments arranged by size: 

                2468    1696     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PstI     PvuI    PvuII     SacI     SalI    SanDI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII    EcoRV
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII    SacII
     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com