(Circular) (Six-base) MAPSORT of: pFN21AB8258.seq Check: 1046 from: 1 to: 5422 pFN21AB8258 5422 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2425 2425 Size: 5422 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3232 278 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2765 1937 Size: 828 4594 Acc65I G'GTAC_C Cuts at: 2743 2743 Size: 5422 AclI AA'CG_TT Cuts at: 1563 3549 3922 1563 Size: 1986 373 3063 Fragments arranged by size: 3063 1986 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3485 4869 5137 1632 Size: 1853 1384 268 1917 Fragments arranged by size: 1917 1853 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5225 AflIII A'CryG_T Cuts at: 1203 4321 5298 1203 Size: 3118 977 1327 Fragments arranged by size: 3118 1327 977 AgeI A'CCGG_T Cuts at: 4253 4253 Size: 5422 AhdI GACnn_n'nnGTC Cuts at: 4151 4151 Size: 5422 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5422 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2496 2589 1184 Size: 1312 93 4017 Fragments arranged by size: 4017 1312 93 AlwNI CAG_nnn'CTG Cuts at: 2255 2273 3165 4737 2255 Size: 18 892 1572 2940 Fragments arranged by size: 2940 1572 892 18 ApaLI G'TGCA_C Cuts at: 3479 4635 3479 Size: 1156 4266 ApoI r'AATT_y Cuts at: 1493 2693 2731 2957 3109 5218 1493 Size: 1200 38 226 152 2109 1697 Fragments arranged by size: 2109 1697 1200 226 152 38 AseI AT'TA_AT Cuts at: 160 1032 3976 160 Size: 872 2944 1606 Fragments arranged by size: 2944 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 AvaI C'yCGr_G Cuts at: 1870 1943 2028 2747 1870 Size: 73 85 719 4545 Fragments arranged by size: 4545 719 85 73 BamHI G'GATC_C Cuts at: 2752 3119 2752 Size: 367 5055 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2743 4198 618 Size: 325 217 627 956 1455 1842 Fragments arranged by size: 1842 1455 956 627 325 217 BanII G_rGCy'C Cuts at: 1391 2504 2741 1391 Size: 1113 237 4072 Fragments arranged by size: 4072 1113 237 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2388 928 Size: 971 489 3962 Fragments arranged by size: 3962 971 489 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3613 3647 1903 Size: 34 1676 34 3678 Fragments arranged by size: 3678 1676 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3314 4524 3314 Size: 1210 4212 BclI T'GATC_A Cuts at: 1548 1548 Size: 5422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4033 136 Size: 107 122 71 631 605 337 2024 1525 Fragments arranged by size: 2024 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5417 5417 Size: 5422 BlpI GC'TnA_GC Cuts at: 2248 2839 2248 Size: 591 4831 Bme1580I G_kGCm'C Cuts at: 1790 3483 4639 1790 Size: 1693 1156 2573 Fragments arranged by size: 2573 1693 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5422 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2089 2112 2181 Size: 416 53 83 399 24 672 23 69 Cuts at: 2181 3153 4111 442 Size: 972 958 1753 Fragments arranged by size: 1753 972 958 672 416 399 83 69 53 24 23 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2058 2143 2596 4082 1239 Size: 819 85 453 1486 2579 Fragments arranged by size: 2579 1486 819 453 85 Bpu10I CC'TnA_GC Cuts at: 2242 2242 Size: 5422 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2406 3547 4412 4710 2406 Size: 1141 865 298 3118 Fragments arranged by size: 3118 1141 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2673 4085 882 Size: 617 1174 1412 2219 Fragments arranged by size: 2219 1412 1174 617 BsaAI yAC'GTr Cuts at: 493 1204 3213 493 Size: 711 2009 2702 Fragments arranged by size: 2702 2009 711 BsaBI GATnn'nnATC Cuts at: 5416 5416 Size: 5422 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3229 3611 4246 275 Size: 53 83 186 936 1696 382 635 1451 Fragments arranged by size: 1696 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3855 4253 4527 4674 5085 3855 Size: 398 274 147 411 4192 Fragments arranged by size: 4192 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2421 2451 1178 Size: 30 165 30 1018 30 4149 Fragments arranged by size: 4149 1018 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2634 1186 Size: 1448 3974 BseYI C'CCAG_C Cuts at: 2412 4625 5121 2412 Size: 2213 496 2713 Fragments arranged by size: 2713 2213 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2144 1163 Size: 573 408 4441 Fragments arranged by size: 4441 573 408 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2224 2872 3633 4661 1540 Size: 275 186 223 648 761 1028 2301 Fragments arranged by size: 2301 1028 761 648 275 223 186 BsiHKAI G_wGCw'C Cuts at: 2504 3483 3568 4639 2504 Size: 979 85 1071 3287 Fragments arranged by size: 3287 1071 979 85 BsmI GAATG_Cn' Cuts at: 2933 3026 2933 Size: 93 5329 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2504 2741 3483 3568 4639 1391 Size: 399 714 237 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 714 399 237 85 BspEI T'CCGG_A Cuts at: 5085 5085 Size: 5422 BspHI T'CATG_A Cuts at: 3311 5041 3311 Size: 1730 3692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2425 2777 844 Size: 1581 352 3489 Fragments arranged by size: 3489 1581 352 BsrBI CCG'CTC Cuts at: 1394 1823 2602 2662 3309 1394 Size: 429 779 60 647 3507 Fragments arranged by size: 3507 779 647 429 60 BsrDI GCAATG_nn' Cuts at: 65 1248 2640 3917 4091 65 Size: 1183 1392 1277 174 1396 Fragments arranged by size: 1396 1392 1277 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2047 4066 4253 1700 Size: 110 237 2019 187 2869 Fragments arranged by size: 2869 2019 237 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5422 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5422 BssSI C'ACGA_G Cuts at: 3482 4494 3482 Size: 1012 4410 BstAPI GCAn_nnn'nTGC Cuts at: 794 2232 2642 794 Size: 1438 410 3574 Fragments arranged by size: 3574 1438 410 BstBI TT'CG_AA Cuts at: 4226 4226 Size: 5422 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5422 BstXI CCAn_nnnn'nTGG Cuts at: 2116 2116 Size: 5422 BstYI r'GATC_y Cuts at: 1972 2752 3119 3504 3521 4973 5059 5071 5417 Size: 780 367 385 17 1452 86 12 346 Cuts at: 5417 1972 Size: 1977 Fragments arranged by size: 1977 1452 780 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2595 513 Size: 552 939 591 3340 Fragments arranged by size: 3340 939 591 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2167 2389 487 Size: 683 56 941 222 3520 Fragments arranged by size: 3520 941 683 222 56 BtsI GCAGTG_nn' Cuts at: 2571 2938 3732 3752 2571 Size: 367 794 20 4241 Fragments arranged by size: 4241 794 367 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5422 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5387 DraI TTT'AAA Cuts at: 2726 2726 Size: 5422 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4429 809 Size: 507 3113 1802 Fragments arranged by size: 3113 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 2592 2869 Size: 54 1425 50 275 18 288 474 277 Cuts at: 2869 3758 5387 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 474 288 277 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2869 1537 Size: 275 1057 4090 Fragments arranged by size: 4090 1057 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2550 3352 1353 Size: 1197 802 3423 Fragments arranged by size: 3423 1197 802 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2168 2618 4009 4383 4529 387 Size: 1064 138 579 450 1391 374 146 1280 Fragments arranged by size: 1391 1280 1064 579 450 374 146 138 EcoICRI GAG'CTC Cuts at: 2502 2502 Size: 5422 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2143 2596 3485 4082 4869 5137 Size: 393 426 85 453 889 597 787 268 Cuts at: 5137 1239 Size: 1524 Fragments arranged by size: 1524 889 787 597 453 426 393 268 85 EcoNI CCTnn'n_nnAGG Cuts at: 2354 2424 2354 Size: 70 5352 EcoO109I rG'GnC_Cy Cuts at: 2175 2416 2175 Size: 241 5181 EcoRI G'AATT_C Cuts at: 2731 3109 2731 Size: 378 5044 FspI TGC'GCA Cuts at: 3928 3928 Size: 5422 HaeII r_GCGC'y Cuts at: 1678 4569 1678 Size: 2891 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2610 2642 4063 4095 4137 Size: 32 135 32 1038 32 1421 32 42 Cuts at: 4137 4169 1373 Size: 32 2626 Fragments arranged by size: 2626 1421 1038 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2766 3012 669 Size: 988 281 828 246 3079 Fragments arranged by size: 3079 988 828 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5422 HpaI GTT'AAC Cuts at: 3012 3012 Size: 5422 KpnI G_GTAC'C Cuts at: 2747 2747 Size: 5422 MfeI C'AATT_G Cuts at: 3021 3021 Size: 5422 MluI A'CGCG_T Cuts at: 5298 5298 Size: 5422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4536 4720 1305 Size: 3231 184 2007 Fragments arranged by size: 3231 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2594 10 Size: 54 1425 343 762 2838 Fragments arranged by size: 2838 1425 762 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 2120 3380 3739 3898 518 Size: 648 948 6 1260 359 159 2042 Fragments arranged by size: 2042 1260 948 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2252 2838 3515 4663 4908 1606 Size: 646 586 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 646 586 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2595 513 Size: 552 939 591 3340 Fragments arranged by size: 3340 939 591 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5422 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5422 NotI GC'GGCC_GC Cuts at: 2869 2869 Size: 5422 NspI r_CATG'y Cuts at: 1132 1229 2780 4325 1132 Size: 97 1551 1545 2229 Fragments arranged by size: 2229 1551 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5422 PciI A'CATG_T Cuts at: 4321 4321 Size: 5422 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5422 PfoI T'CCnGG_A Cuts at: 2495 2495 Size: 5422 PmeI GTTT'AAAC Cuts at: 2726 2726 Size: 5422 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3491 3523 5004 5036 3491 Size: 32 1481 32 3877 Fragments arranged by size: 3877 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2175 2416 2175 Size: 241 5181 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5422 PsiI TTA'TAA Cuts at: 2992 2992 Size: 5422 PstI C_TGCA'G Cuts at: 830 2774 830 Size: 1944 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5422 PvuII CAG'CTG Cuts at: 2252 2252 Size: 5422 SacI G_AGCT'C Cuts at: 2504 2504 Size: 5422 SalI G'TCGA_C Cuts at: 1936 2764 1936 Size: 828 4594 SanDI GG'GwC_CC Cuts at: 2416 2416 Size: 5422 SbfI CC_TGCA'GG Cuts at: 2774 2774 Size: 5422 ScaI AGT'ACT Cuts at: 2670 3670 5382 2670 Size: 1000 1712 2710 Fragments arranged by size: 2710 1712 1000 SfcI C'TryA_G Cuts at: 826 1045 2770 3905 4586 4777 826 Size: 219 1725 1135 681 191 1471 Fragments arranged by size: 1725 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5422 SmaI CCC'GGG Cuts at: 1872 2749 1872 Size: 877 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2421 3526 4427 4689 820 Size: 197 926 478 1105 901 262 1553 Fragments arranged by size: 1553 1105 926 901 478 262 197 SnaBI TAC'GTA Cuts at: 493 3213 493 Size: 2720 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5422 SphI G_CATG'C Cuts at: 1132 2780 1132 Size: 1648 3774 SspI AAT'ATT Cuts at: 5 52 3346 5 Size: 47 3294 2081 Fragments arranged by size: 3294 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5422 StyI C'CwwG_G Cuts at: 513 1065 2004 2550 2595 2861 513 Size: 552 939 546 45 266 3074 Fragments arranged by size: 3074 939 552 546 266 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3449 3466 3619 3804 4249 325 Size: 1255 1869 17 153 185 445 1498 Fragments arranged by size: 1869 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2439 2668 3668 Size: 274 80 33 51 1443 462 229 1000 Cuts at: 3668 5380 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1000 462 274 229 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2421 2453 5382 5414 2421 Size: 32 2929 32 2429 Fragments arranged by size: 2929 2429 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5422 XbaI T'CTAG_A Cuts at: 2758 2758 Size: 5422 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2049 2049 Size: 5422 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5422 XmaI C'CCGG_G Cuts at: 1870 2747 1870 Size: 877 4545 XmnI GAAnn'nnTTC Cuts at: 3551 3551 Size: 5422 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3230 276 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI