(Circular) (Six-base) MAPSORT of: pFN21AB8247.seq Check: 7699 from: 1 to: 5476 pFN21AB8247 5476 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3286 278 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2077 2397 2819 1937 Size: 140 320 422 4594 Fragments arranged by size: 4594 422 320 140 Acc65I G'GTAC_C Cuts at: 2797 2797 Size: 5476 AclI AA'CG_TT Cuts at: 1563 3603 3976 1563 Size: 2040 373 3063 Fragments arranged by size: 3063 2040 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3539 4923 5191 1632 Size: 1907 1384 268 1917 Fragments arranged by size: 1917 1907 1384 268 AfeI AGC'GCT Cuts at: 2222 2222 Size: 5476 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5279 AflIII A'CryG_T Cuts at: 1203 4375 5352 1203 Size: 3172 977 1327 Fragments arranged by size: 3172 1327 977 AgeI A'CCGG_T Cuts at: 4307 4307 Size: 5476 AhdI GACnn_n'nnGTC Cuts at: 4205 4205 Size: 5476 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5476 AlwNI CAG_nnn'CTG Cuts at: 3219 4791 3219 Size: 1572 3904 ApaI G_GGCC'C Cuts at: 2182 2660 2182 Size: 478 4998 ApaLI G'TGCA_C Cuts at: 3533 4689 3533 Size: 1156 4320 ApoI r'AATT_y Cuts at: 1493 2785 3011 3163 5272 1493 Size: 1292 226 152 2109 1697 Fragments arranged by size: 2109 1697 1292 226 152 AseI AT'TA_AT Cuts at: 160 1032 4030 160 Size: 872 2998 1606 Fragments arranged by size: 2998 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 AvaI C'yCGr_G Cuts at: 1870 1943 2273 2801 1870 Size: 73 330 528 4545 Fragments arranged by size: 4545 528 330 73 BamHI G'GATC_C Cuts at: 2592 2806 3173 2592 Size: 214 367 4895 Fragments arranged by size: 4895 367 214 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2797 4252 618 Size: 325 217 627 1010 1455 1842 Fragments arranged by size: 1842 1455 1010 627 325 217 BanII G_rGCy'C Cuts at: 1391 2103 2182 2660 2795 1391 Size: 712 79 478 135 4072 Fragments arranged by size: 4072 712 478 135 79 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2709 928 Size: 971 810 3695 Fragments arranged by size: 3695 971 810 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3667 3701 1903 Size: 34 1730 34 3678 Fragments arranged by size: 3678 1730 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3368 4578 3368 Size: 1210 4266 BclI T'GATC_A Cuts at: 1548 1548 Size: 5476 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4087 136 Size: 107 122 71 631 605 2415 1525 Fragments arranged by size: 2415 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5471 5471 Size: 5476 BlpI GC'TnA_GC Cuts at: 2893 2893 Size: 5476 Bme1580I G_kGCm'C Cuts at: 1790 2182 2660 3537 4693 1790 Size: 392 478 877 1156 2573 Fragments arranged by size: 2573 1156 877 478 392 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5476 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2405 3207 4165 Size: 416 53 83 399 24 988 802 958 Cuts at: 4165 442 Size: 1753 Fragments arranged by size: 1753 988 958 802 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5476 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2189 2441 2783 4136 1239 Size: 878 72 252 342 1353 2579 Fragments arranged by size: 2579 1353 878 342 252 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2003 2066 3601 4466 4764 2003 Size: 63 1535 865 298 2715 Fragments arranged by size: 2715 1535 865 298 63 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4139 882 Size: 617 2640 2219 Fragments arranged by size: 2640 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3267 493 Size: 711 2063 2702 Fragments arranged by size: 2702 2063 711 BsaBI GATnn'nnATC Cuts at: 5470 5470 Size: 5476 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3283 3665 4300 275 Size: 53 83 186 936 1750 382 635 1451 Fragments arranged by size: 1750 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3909 4307 4581 4728 5139 3909 Size: 398 274 147 411 4246 Fragments arranged by size: 4246 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2195 2225 1178 Size: 30 165 30 792 30 4429 Fragments arranged by size: 4429 792 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2303 1186 Size: 1117 4359 BseYI C'CCAG_C Cuts at: 2071 2232 2540 4679 5175 2071 Size: 161 308 2139 496 2372 Fragments arranged by size: 2372 2139 496 308 161 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4903 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2926 3687 4715 1540 Size: 275 186 925 761 1028 2301 Fragments arranged by size: 2301 1028 925 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2103 3537 3622 4693 2103 Size: 1434 85 1071 2886 Fragments arranged by size: 2886 1434 1071 85 BsmI GAATG_Cn' Cuts at: 2987 3080 2987 Size: 93 5383 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2182 2660 2795 3537 3622 4693 Size: 399 313 79 478 135 742 85 1071 Cuts at: 4693 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 478 399 313 135 85 79 BspEI T'CCGG_A Cuts at: 5139 5139 Size: 5476 BspHI T'CATG_A Cuts at: 3365 5095 3365 Size: 1730 3746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2831 844 Size: 1987 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2365 3363 1394 Size: 429 542 998 3507 Fragments arranged by size: 3507 998 542 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3971 4145 65 Size: 1183 2723 174 1396 Fragments arranged by size: 2723 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4120 4307 1700 Size: 110 2310 187 2869 Fragments arranged by size: 2869 2310 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5476 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5476 BssSI C'ACGA_G Cuts at: 3536 4548 3536 Size: 1012 4464 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5476 BstBI TT'CG_AA Cuts at: 4280 4280 Size: 5476 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5476 BstXI CCAn_nnnn'nTGG Cuts at: 2728 2728 Size: 5476 BstYI r'GATC_y Cuts at: 1972 2592 2806 3173 3558 3575 5027 5113 5125 Size: 620 214 367 385 17 1452 86 12 Cuts at: 5125 5471 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 620 385 367 346 214 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4924 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4737 Fragments arranged by size: 4737 683 56 BtsI GCAGTG_nn' Cuts at: 2992 3786 3806 2992 Size: 794 20 4662 Fragments arranged by size: 4662 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5476 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5441 DraI TTT'AAA Cuts at: 2780 2780 Size: 5476 DraIII CAC_nnn'GTG Cuts at: 2413 2413 Size: 5476 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4483 809 Size: 507 3167 1802 Fragments arranged by size: 3167 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2463 2730 2923 Size: 54 1425 50 275 18 633 267 193 Cuts at: 2923 3812 5441 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 633 275 267 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2923 1537 Size: 275 1111 4090 Fragments arranged by size: 4090 1111 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2061 2321 2603 3406 1353 Size: 708 260 282 803 3423 Fragments arranged by size: 3423 803 708 282 260 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4063 4437 4583 387 Size: 1064 138 2474 374 146 1280 Fragments arranged by size: 2474 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2101 2101 Size: 5476 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2189 2441 2783 3539 4136 4923 Size: 393 485 72 252 342 756 597 787 Cuts at: 4923 5191 1239 Size: 268 1524 Fragments arranged by size: 1524 787 756 597 485 393 342 268 252 72 EcoNI CCTnn'n_nnAGG Cuts at: 2198 2435 2465 2198 Size: 237 30 5209 Fragments arranged by size: 5209 237 30 EcoO109I rG'GnC_Cy Cuts at: 2178 2650 2657 2178 Size: 472 7 4997 Fragments arranged by size: 4997 472 7 EcoRI G'AATT_C Cuts at: 2785 3163 2785 Size: 378 5098 FspI TGC'GCA Cuts at: 3982 3982 Size: 5476 HaeII r_GCGC'y Cuts at: 1678 2224 4623 1678 Size: 546 2399 2531 Fragments arranged by size: 2531 2399 546 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 2634 2666 4117 Size: 32 135 32 623 32 407 32 1451 Cuts at: 4117 4149 4191 4223 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1451 623 407 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2078 2820 3066 669 Size: 988 281 140 742 246 3079 Fragments arranged by size: 3079 988 742 281 246 140 HindIII A'AGCT_T Cuts at: 748 748 Size: 5476 HpaI GTT'AAC Cuts at: 3066 3066 Size: 5476 KpnI G_GTAC'C Cuts at: 2801 2801 Size: 5476 MfeI C'AATT_G Cuts at: 3075 3075 Size: 5476 MluI A'CGCG_T Cuts at: 5352 5352 Size: 5476 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2340 4590 4774 1305 Size: 1035 2250 184 2007 Fragments arranged by size: 2250 2007 1035 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2465 2732 10 Size: 54 1425 343 633 267 2754 Fragments arranged by size: 2754 1425 633 343 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 3434 3793 3952 518 Size: 648 2268 359 159 2042 Fragments arranged by size: 2268 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2761 2892 3569 4717 4962 1606 Size: 1155 131 677 1148 245 2120 Fragments arranged by size: 2120 1155 1148 677 245 131 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4924 NdeI CA'TA_TG Cuts at: 387 387 Size: 5476 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5476 NotI GC'GGCC_GC Cuts at: 2923 2923 Size: 5476 NspI r_CATG'y Cuts at: 1132 1229 2834 4379 1132 Size: 97 1605 1545 2229 Fragments arranged by size: 2229 1605 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5476 PciI A'CATG_T Cuts at: 4375 4375 Size: 5476 PflMI CCAn_nnn'nTGG Cuts at: 1108 2299 1108 Size: 1191 4285 PmeI GTTT'AAAC Cuts at: 2780 2780 Size: 5476 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3545 3577 5058 5090 3545 Size: 32 1481 32 3931 Fragments arranged by size: 3931 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2650 2650 Size: 5476 PshAI GACnn'nnGTC Cuts at: 1613 2393 1613 Size: 780 4696 PsiI TTA'TAA Cuts at: 3046 3046 Size: 5476 PspOMI G'GGCC_C Cuts at: 2178 2656 2178 Size: 478 4998 PstI C_TGCA'G Cuts at: 830 2828 830 Size: 1998 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5476 PvuII CAG'CTG Cuts at: 2761 2761 Size: 5476 SacI G_AGCT'C Cuts at: 2103 2103 Size: 5476 SalI G'TCGA_C Cuts at: 1936 2076 2818 1936 Size: 140 742 4594 Fragments arranged by size: 4594 742 140 SapI GCTCTTCn'nnn_ Cuts at: 2061 2061 Size: 5476 SbfI CC_TGCA'GG Cuts at: 2828 2828 Size: 5476 ScaI AGT'ACT Cuts at: 3724 5436 3724 Size: 1712 3764 SfcI C'TryA_G Cuts at: 826 1045 2671 2824 3959 4640 4831 826 Size: 219 1626 153 1135 681 191 1471 Fragments arranged by size: 1626 1471 1135 681 219 191 153 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5476 SmaI CCC'GGG Cuts at: 1872 2803 1872 Size: 931 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2018 2081 3580 4481 4743 820 Size: 197 926 75 63 1499 901 262 1553 Fragments arranged by size: 1553 1499 926 901 262 197 75 63 SnaBI TAC'GTA Cuts at: 493 3267 493 Size: 2774 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5476 SphI G_CATG'C Cuts at: 1132 2834 1132 Size: 1702 3774 SspI AAT'ATT Cuts at: 5 52 3400 5 Size: 47 3348 2081 Fragments arranged by size: 3348 2081 47 StuI AGG'CCT Cuts at: 1517 2558 1517 Size: 1041 4435 StyI C'CwwG_G Cuts at: 513 1065 2376 2466 2915 513 Size: 552 1311 90 449 3074 Fragments arranged by size: 3074 1311 552 449 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2424 2532 2626 3503 3520 3673 3858 Size: 1255 844 108 94 877 17 153 185 Cuts at: 3858 4303 325 Size: 445 1498 Fragments arranged by size: 1498 1255 877 844 445 185 153 108 94 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3722 5434 96 Size: 274 80 33 51 1443 1745 1712 138 Fragments arranged by size: 1745 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2399 2431 2698 2730 5436 5468 2399 Size: 32 267 32 2706 32 2407 Fragments arranged by size: 2706 2407 267 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5476 XbaI T'CTAG_A Cuts at: 2812 2812 Size: 5476 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5476 XmaI C'CCGG_G Cuts at: 1870 2801 1870 Size: 931 4545 XmnI GAAnn'nnTTC Cuts at: 3605 3605 Size: 5476 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3284 276 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI