Restriction map for pFN21AB8243
 (Circular) (Six-base) MAPSORT of: pFN21AB8243.seq  Check: 6313  from: 1  to: 5353



pFN21AB8243  5353 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:41  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3163     278 
   Size:         53      83     186     936    1627    2468

  Fragments arranged by size: 

                2468    1627     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2696    1937 
   Size:        759    4594
 
Acc65I G'GTAC_C

Cuts at:   2674    2674 
   Size:       5353
 
AclI AA'CG_TT

Cuts at:   1563    3480    3853    1563 
   Size:       1917     373    3063

  Fragments arranged by size: 

                3063    1917     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2420    3416    4800    5068    1632 
   Size:        788     996    1384     268    1917

  Fragments arranged by size: 

                1917    1384     996     788     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5156
 
AflIII A'CryG_T

Cuts at:   1203    4252    5229    1203 
   Size:       3049     977    1327

  Fragments arranged by size: 

                3049    1327     977
 
AgeI A'CCGG_T

Cuts at:   4184    4184 
   Size:       5353
 
AhdI GACnn_n'nnGTC

Cuts at:   4082    4082 
   Size:       5353
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5353
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5353
 
AlwNI CAG_nnn'CTG

Cuts at:   3096    4668    3096 
   Size:       1572    3781
 
ApaLI G'TGCA_C

Cuts at:   3410    4566    3410 
   Size:       1156    4197
 
ApoI r'AATT_y

Cuts at:   1493    2105    2216    2528    2662    2888    3040    5149    1493 
   Size:        612     111     312     134     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     612     312     226     152     134     111
            
 
AseI AT'TA_AT

Cuts at:    160    1032    3907     160 
   Size:        872    2875    1606

  Fragments arranged by size: 

                2875    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5353
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2678    1870 
   Size:         73     735    4545

  Fragments arranged by size: 

                4545     735      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2321    2354    2321 
   Size:         33    5320
 
BamHI G'GATC_C

Cuts at:   2683    3050    2683 
   Size:        367    4986
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2674    4129     618 
   Size:        325     217     627     887    1455    1842

  Fragments arranged by size: 

                1842    1455     887     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2025    2672    1391 
   Size:        634     647    4072

  Fragments arranged by size: 

                4072     647     634
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2407     928 
   Size:        971     508    3874

  Fragments arranged by size: 

                3874     971     508
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2435    2469    3544    3578    1903 
   Size:         34     498      34    1075      34    3678

  Fragments arranged by size: 

                3678    1075     498      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3245    4455    3245 
   Size:       1210    4143
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5353
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2314    3964     136 
   Size:        107     122      71     631     605     642    1650    1525

  Fragments arranged by size: 

                1650    1525     642     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2323    5348    2323 
   Size:       3025    2328
 
BlpI GC'TnA_GC

Cuts at:   2770    2770 
   Size:       5353
 
Bme1580I G_kGCm'C

Cuts at:   1790    3414    4570    1790 
   Size:       1624    1156    2573

  Fragments arranged by size: 

                2573    1624    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5353
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2248    3084    4042 
   Size:        416      53      83     399      24     831     836     958

Cuts at:   4042     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     836     831     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5353
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4013    1239 
   Size:       2774    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3478    4343    4641    3478 
   Size:        865     298    4190

  Fragments arranged by size: 

                4190     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2508    4016     882 
   Size:        617    1009    1508    2219

  Fragments arranged by size: 

                2219    1508    1009     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3144     493 
   Size:        711    1940    2702

  Fragments arranged by size: 

                2702    1940     711
 
BsaBI GATnn'nnATC

Cuts at:   5347    5347 
   Size:       5353
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3160    3542    4177     275 
   Size:         53      83     186     936    1627     382     635    1451

  Fragments arranged by size: 

                1627    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3786    4184    4458    4605    5016    3786 
   Size:        398     274     147     411    4123

  Fragments arranged by size: 

                4123     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5128

  Fragments arranged by size: 

                5128     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5353
 
BseYI C'CCAG_C

Cuts at:   2575    4556    5052    2575 
   Size:       1981     496    2876

  Fragments arranged by size: 

                2876    1981     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4780
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2803    3564    4592    1540 
   Size:        275     186     802     761    1028    2301

  Fragments arranged by size: 

                2301    1028     802     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2601    3414    3499    4570    2601 
   Size:        813      85    1071    3384

  Fragments arranged by size: 

                3384    1071     813      85
 
BsmI GAATG_Cn'

Cuts at:   2864    2957    2864 
   Size:         93    5260
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2025    2601    2672    3414    3499    4570    1391 
   Size:        399     235     576      71     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     576     399     235      85      71
            
 
BspEI T'CCGG_A

Cuts at:   5016    5016 
   Size:       5353
 
BspHI T'CATG_A

Cuts at:   3242    4972    3242 
   Size:       1730    3623
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2708     844 
   Size:       1864    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3240    1394 
   Size:        429    1417    3507

  Fragments arranged by size: 

                3507    1417     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3848    4022      65 
   Size:       1183    2600     174    1396

  Fragments arranged by size: 

                2600    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2204    3997    4184    1700 
   Size:        110     394    1793     187    2869

  Fragments arranged by size: 

                2869    1793     394     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5353
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5353
 
BssSI C'ACGA_G

Cuts at:   3413    4425    3413 
   Size:       1012    4341
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5353
 
BstBI TT'CG_AA

Cuts at:   4157    4157 
   Size:       5353
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5353
 
BstYI r'GATC_y

Cuts at:   1972    2323    2683    3050    3435    3452    4904    4990    5002 
   Size:        351     360     367     385      17    1452      86      12

Cuts at:   5002    5348    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     385     367     360     351     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3862

  Fragments arranged by size: 

                3862     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4614

  Fragments arranged by size: 

                4614     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2869    3663    3683    2869 
   Size:        794      20    4539

  Fragments arranged by size: 

                4539     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5353
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5318
 
DraI TTT'AAA

Cuts at:   2657    2657 
   Size:       5353
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4360     809 
   Size:        507    3044    1802

  Fragments arranged by size: 

                3044    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2206    2800    3689 
   Size:         54    1425      50     275      18     376     594     889

Cuts at:   3689    5318       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     594     376     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2800    1537 
   Size:        275     988    4090

  Fragments arranged by size: 

                4090     988     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3283    1353 
   Size:       1930    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3940    4314    4460     387 
   Size:       1064     138    2351     374     146    1280

  Fragments arranged by size: 

                2351    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2420    3416    4013    4800    5068    1239 
   Size:        393     788     996     597     787     268    1524

  Fragments arranged by size: 

                1524     996     788     787     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2174    2174 
   Size:       5353
 
EcoRI G'AATT_C

Cuts at:   2662    3040    2662 
   Size:        378    4975
 
FspI TGC'GCA

Cuts at:   2013    3859    2013 
   Size:       1846    3507
 
HaeII r_GCGC'y

Cuts at:   1678    4500    1678 
   Size:       2822    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2267    2299    2611    2643    3994 
   Size:         32     135      32     695      32     312      32    1351

Cuts at:   3994    4026    4068    4100    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1351     695     312     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2697    2943     669 
   Size:        988     281     759     246    3079

  Fragments arranged by size: 

                3079     988     759     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5353
 
HpaI GTT'AAC

Cuts at:   2943    2943 
   Size:       5353
 
KpnI G_GTAC'C

Cuts at:   2678    2678 
   Size:       5353
 
MfeI C'AATT_G

Cuts at:   2952    2952 
   Size:       5353
 
MluI A'CGCG_T

Cuts at:   5229    5229 
   Size:       5353
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4467    4651    1305 
   Size:       3162     184    2007

  Fragments arranged by size: 

                3162    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3531

  Fragments arranged by size: 

                3531    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2267    3311    3670    3829     518 
   Size:        648    1101    1044     359     159    2042

  Fragments arranged by size: 

                2042    1101    1044     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2769    3446    4594    4839    1606 
   Size:       1163     677    1148     245    2120

  Fragments arranged by size: 

                2120    1163    1148     677     245
 
NaeI GCC'GGC

Cuts at:   2206    2206 
   Size:       5353
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3862

  Fragments arranged by size: 

                3862     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5353
 
NgoMIV G'CCGG_C

Cuts at:   2204    2204 
   Size:       5353
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5353
 
NotI GC'GGCC_GC

Cuts at:   2800    2800 
   Size:       5353
 
NspI r_CATG'y

Cuts at:   1132    1229    2711    4256    1132 
   Size:         97    1482    1545    2229

  Fragments arranged by size: 

                2229    1545    1482      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5353
 
PciI A'CATG_T

Cuts at:   4252    4252 
   Size:       5353
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5353
 
PfoI T'CCnGG_A

Cuts at:   2631    2631 
   Size:       5353
 
PmeI GTTT'AAAC

Cuts at:   2657    2657 
   Size:       5353
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3422    3454    4935    4967    3422 
   Size:         32    1481      32    3808

  Fragments arranged by size: 

                3808    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2174    2174 
   Size:       5353
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5353
 
PsiI TTA'TAA

Cuts at:   2923    2923 
   Size:       5353
 
PstI C_TGCA'G

Cuts at:    830    2705     830 
   Size:       1875    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5353
 
SalI G'TCGA_C

Cuts at:   1936    2695    1936 
   Size:        759    4594
 
SbfI CC_TGCA'GG

Cuts at:   2705    2705 
   Size:       5353
 
ScaI AGT'ACT

Cuts at:   2441    3601    5313    2441 
   Size:       1160    1712    2481

  Fragments arranged by size: 

                2481    1712    1160
 
SexAI A'CCwGG_T

Cuts at:   2649    2649 
   Size:       5353
 
SfcI C'TryA_G

Cuts at:    826    1045    2701    3836    4517    4708     826 
   Size:        219    1656    1135     681     191    1471

  Fragments arranged by size: 

                1656    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5353
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5353
 
SmaI CCC'GGG

Cuts at:   1872    2680    1872 
   Size:        808    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3457    4358    4620     820 
   Size:        197     926    1514     901     262    1553

  Fragments arranged by size: 

                1553    1514     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3144     493 
   Size:       2651    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5353
 
SphI G_CATG'C

Cuts at:   1132    2711    1132 
   Size:       1579    3774
 
SspI AAT'ATT

Cuts at:      5      52    3277       5 
   Size:         47    3225    2081

  Fragments arranged by size: 

                3225    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5353
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2130    2792     513 
   Size:        552     939     126     662    3074

  Fragments arranged by size: 

                3074     939     662     552     126
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2123    3380    3397    3550    3735    4180     325 
   Size:       1255     543    1257      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1257    1255     543     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2439    3599    5311 
   Size:        274      80      33      51    1443     462    1160    1712

Cuts at:   5311      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1160     462     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5313    5345    5313 
   Size:         32    5321
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5353
 
XbaI T'CTAG_A

Cuts at:   2689    2689 
   Size:       5353
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5353
 
XmaI C'CCGG_G

Cuts at:   1870    2678    1870 
   Size:        808    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3482    3482 
   Size:       5353
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3161     276 
   Size:         53      83     186     936    1627    2468

  Fragments arranged by size: 

                2468    1627     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI
     PvuI     SalI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI
     NarI     NruI     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI
    PvuII    RsrII     SacI    SacII    SanDI     SapI     SfiI     SfoI
     SrfI     SwaI     XcmI
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