(Circular) (Six-base) MAPSORT of: pFN21AB8243.seq Check: 6313 from: 1 to: 5353 pFN21AB8243 5353 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3163 278 Size: 53 83 186 936 1627 2468 Fragments arranged by size: 2468 1627 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2696 1937 Size: 759 4594 Acc65I G'GTAC_C Cuts at: 2674 2674 Size: 5353 AclI AA'CG_TT Cuts at: 1563 3480 3853 1563 Size: 1917 373 3063 Fragments arranged by size: 3063 1917 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2420 3416 4800 5068 1632 Size: 788 996 1384 268 1917 Fragments arranged by size: 1917 1384 996 788 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5156 AflIII A'CryG_T Cuts at: 1203 4252 5229 1203 Size: 3049 977 1327 Fragments arranged by size: 3049 1327 977 AgeI A'CCGG_T Cuts at: 4184 4184 Size: 5353 AhdI GACnn_n'nnGTC Cuts at: 4082 4082 Size: 5353 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5353 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5353 AlwNI CAG_nnn'CTG Cuts at: 3096 4668 3096 Size: 1572 3781 ApaLI G'TGCA_C Cuts at: 3410 4566 3410 Size: 1156 4197 ApoI r'AATT_y Cuts at: 1493 2105 2216 2528 2662 2888 3040 5149 1493 Size: 612 111 312 134 226 152 2109 1697 Fragments arranged by size: 2109 1697 612 312 226 152 134 111 AseI AT'TA_AT Cuts at: 160 1032 3907 160 Size: 872 2875 1606 Fragments arranged by size: 2875 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5353 AvaI C'yCGr_G Cuts at: 1870 1943 2678 1870 Size: 73 735 4545 Fragments arranged by size: 4545 735 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2321 2354 2321 Size: 33 5320 BamHI G'GATC_C Cuts at: 2683 3050 2683 Size: 367 4986 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2674 4129 618 Size: 325 217 627 887 1455 1842 Fragments arranged by size: 1842 1455 887 627 325 217 BanII G_rGCy'C Cuts at: 1391 2025 2672 1391 Size: 634 647 4072 Fragments arranged by size: 4072 647 634 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2407 928 Size: 971 508 3874 Fragments arranged by size: 3874 971 508 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2435 2469 3544 3578 1903 Size: 34 498 34 1075 34 3678 Fragments arranged by size: 3678 1075 498 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3245 4455 3245 Size: 1210 4143 BclI T'GATC_A Cuts at: 1548 1548 Size: 5353 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2314 3964 136 Size: 107 122 71 631 605 642 1650 1525 Fragments arranged by size: 1650 1525 642 631 605 122 107 71 BglII A'GATC_T Cuts at: 2323 5348 2323 Size: 3025 2328 BlpI GC'TnA_GC Cuts at: 2770 2770 Size: 5353 Bme1580I G_kGCm'C Cuts at: 1790 3414 4570 1790 Size: 1624 1156 2573 Fragments arranged by size: 2573 1624 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5353 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2248 3084 4042 Size: 416 53 83 399 24 831 836 958 Cuts at: 4042 442 Size: 1753 Fragments arranged by size: 1753 958 836 831 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5353 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4013 1239 Size: 2774 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3478 4343 4641 3478 Size: 865 298 4190 Fragments arranged by size: 4190 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2508 4016 882 Size: 617 1009 1508 2219 Fragments arranged by size: 2219 1508 1009 617 BsaAI yAC'GTr Cuts at: 493 1204 3144 493 Size: 711 1940 2702 Fragments arranged by size: 2702 1940 711 BsaBI GATnn'nnATC Cuts at: 5347 5347 Size: 5353 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3160 3542 4177 275 Size: 53 83 186 936 1627 382 635 1451 Fragments arranged by size: 1627 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3786 4184 4458 4605 5016 3786 Size: 398 274 147 411 4123 Fragments arranged by size: 4123 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5128 Fragments arranged by size: 5128 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5353 BseYI C'CCAG_C Cuts at: 2575 4556 5052 2575 Size: 1981 496 2876 Fragments arranged by size: 2876 1981 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4780 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2803 3564 4592 1540 Size: 275 186 802 761 1028 2301 Fragments arranged by size: 2301 1028 802 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2601 3414 3499 4570 2601 Size: 813 85 1071 3384 Fragments arranged by size: 3384 1071 813 85 BsmI GAATG_Cn' Cuts at: 2864 2957 2864 Size: 93 5260 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2601 2672 3414 3499 4570 1391 Size: 399 235 576 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 576 399 235 85 71 BspEI T'CCGG_A Cuts at: 5016 5016 Size: 5353 BspHI T'CATG_A Cuts at: 3242 4972 3242 Size: 1730 3623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2708 844 Size: 1864 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3240 1394 Size: 429 1417 3507 Fragments arranged by size: 3507 1417 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3848 4022 65 Size: 1183 2600 174 1396 Fragments arranged by size: 2600 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2204 3997 4184 1700 Size: 110 394 1793 187 2869 Fragments arranged by size: 2869 1793 394 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5353 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5353 BssSI C'ACGA_G Cuts at: 3413 4425 3413 Size: 1012 4341 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5353 BstBI TT'CG_AA Cuts at: 4157 4157 Size: 5353 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5353 BstYI r'GATC_y Cuts at: 1972 2323 2683 3050 3435 3452 4904 4990 5002 Size: 351 360 367 385 17 1452 86 12 Cuts at: 5002 5348 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 360 351 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3862 Fragments arranged by size: 3862 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4614 Fragments arranged by size: 4614 683 56 BtsI GCAGTG_nn' Cuts at: 2869 3663 3683 2869 Size: 794 20 4539 Fragments arranged by size: 4539 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5353 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5318 DraI TTT'AAA Cuts at: 2657 2657 Size: 5353 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4360 809 Size: 507 3044 1802 Fragments arranged by size: 3044 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2206 2800 3689 Size: 54 1425 50 275 18 376 594 889 Cuts at: 3689 5318 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 594 376 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2800 1537 Size: 275 988 4090 Fragments arranged by size: 4090 988 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3283 1353 Size: 1930 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3940 4314 4460 387 Size: 1064 138 2351 374 146 1280 Fragments arranged by size: 2351 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2420 3416 4013 4800 5068 1239 Size: 393 788 996 597 787 268 1524 Fragments arranged by size: 1524 996 788 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2174 2174 Size: 5353 EcoRI G'AATT_C Cuts at: 2662 3040 2662 Size: 378 4975 FspI TGC'GCA Cuts at: 2013 3859 2013 Size: 1846 3507 HaeII r_GCGC'y Cuts at: 1678 4500 1678 Size: 2822 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2267 2299 2611 2643 3994 Size: 32 135 32 695 32 312 32 1351 Cuts at: 3994 4026 4068 4100 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1351 695 312 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2697 2943 669 Size: 988 281 759 246 3079 Fragments arranged by size: 3079 988 759 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5353 HpaI GTT'AAC Cuts at: 2943 2943 Size: 5353 KpnI G_GTAC'C Cuts at: 2678 2678 Size: 5353 MfeI C'AATT_G Cuts at: 2952 2952 Size: 5353 MluI A'CGCG_T Cuts at: 5229 5229 Size: 5353 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4467 4651 1305 Size: 3162 184 2007 Fragments arranged by size: 3162 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3531 Fragments arranged by size: 3531 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 3311 3670 3829 518 Size: 648 1101 1044 359 159 2042 Fragments arranged by size: 2042 1101 1044 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2769 3446 4594 4839 1606 Size: 1163 677 1148 245 2120 Fragments arranged by size: 2120 1163 1148 677 245 NaeI GCC'GGC Cuts at: 2206 2206 Size: 5353 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3862 Fragments arranged by size: 3862 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5353 NgoMIV G'CCGG_C Cuts at: 2204 2204 Size: 5353 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5353 NotI GC'GGCC_GC Cuts at: 2800 2800 Size: 5353 NspI r_CATG'y Cuts at: 1132 1229 2711 4256 1132 Size: 97 1482 1545 2229 Fragments arranged by size: 2229 1545 1482 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5353 PciI A'CATG_T Cuts at: 4252 4252 Size: 5353 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5353 PfoI T'CCnGG_A Cuts at: 2631 2631 Size: 5353 PmeI GTTT'AAAC Cuts at: 2657 2657 Size: 5353 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3422 3454 4935 4967 3422 Size: 32 1481 32 3808 Fragments arranged by size: 3808 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2174 2174 Size: 5353 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5353 PsiI TTA'TAA Cuts at: 2923 2923 Size: 5353 PstI C_TGCA'G Cuts at: 830 2705 830 Size: 1875 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5353 SalI G'TCGA_C Cuts at: 1936 2695 1936 Size: 759 4594 SbfI CC_TGCA'GG Cuts at: 2705 2705 Size: 5353 ScaI AGT'ACT Cuts at: 2441 3601 5313 2441 Size: 1160 1712 2481 Fragments arranged by size: 2481 1712 1160 SexAI A'CCwGG_T Cuts at: 2649 2649 Size: 5353 SfcI C'TryA_G Cuts at: 826 1045 2701 3836 4517 4708 826 Size: 219 1656 1135 681 191 1471 Fragments arranged by size: 1656 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5353 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5353 SmaI CCC'GGG Cuts at: 1872 2680 1872 Size: 808 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3457 4358 4620 820 Size: 197 926 1514 901 262 1553 Fragments arranged by size: 1553 1514 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3144 493 Size: 2651 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5353 SphI G_CATG'C Cuts at: 1132 2711 1132 Size: 1579 3774 SspI AAT'ATT Cuts at: 5 52 3277 5 Size: 47 3225 2081 Fragments arranged by size: 3225 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5353 StyI C'CwwG_G Cuts at: 513 1065 2004 2130 2792 513 Size: 552 939 126 662 3074 Fragments arranged by size: 3074 939 662 552 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2123 3380 3397 3550 3735 4180 325 Size: 1255 543 1257 17 153 185 445 1498 Fragments arranged by size: 1498 1257 1255 543 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2439 3599 5311 Size: 274 80 33 51 1443 462 1160 1712 Cuts at: 5311 96 Size: 138 Fragments arranged by size: 1712 1443 1160 462 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5313 5345 5313 Size: 32 5321 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5353 XbaI T'CTAG_A Cuts at: 2689 2689 Size: 5353 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5353 XmaI C'CCGG_G Cuts at: 1870 2678 1870 Size: 808 4545 XmnI GAAnn'nnTTC Cuts at: 3482 3482 Size: 5353 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3161 276 Size: 53 83 186 936 1627 2468 Fragments arranged by size: 2468 1627 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI