Restriction map for
pFN21AB8229
(Circular) (Six-base) MAPSORT of: pFN21AB8229.seq Check: 720 from: 1 to: 5209
pFN21AB8229 5209 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3019 278
Size: 53 83 186 936 1483 2468
Fragments arranged by size:
2468 1483 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2552 1937
Size: 615 4594
Acc65I G'GTAC_C
Cuts at: 2530 2530
Size: 5209
AclI AA'CG_TT
Cuts at: 1563 3336 3709 1563
Size: 1773 373 3063
Fragments arranged by size:
3063 1773 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3272 4656 4924 1632
Size: 1640 1384 268 1917
Fragments arranged by size:
1917 1640 1384 268
AfeI AGC'GCT
Cuts at: 2330 2330
Size: 5209
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5012
AflIII A'CryG_T
Cuts at: 1203 4108 5085 1203
Size: 2905 977 1327
Fragments arranged by size:
2905 1327 977
AgeI A'CCGG_T
Cuts at: 4040 4040
Size: 5209
AhdI GACnn_n'nnGTC
Cuts at: 3938 3938
Size: 5209
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5209
AlwNI CAG_nnn'CTG
Cuts at: 2952 4524 2952
Size: 1572 3637
ApaI G_GGCC'C
Cuts at: 2075 2097 2075
Size: 22 5187
ApaLI G'TGCA_C
Cuts at: 3266 4422 3266
Size: 1156 4053
ApoI r'AATT_y
Cuts at: 1493 2518 2744 2896 5005 1493
Size: 1025 226 152 2109 1697
Fragments arranged by size:
2109 1697 1025 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3763 160
Size: 872 2731 1606
Fragments arranged by size:
2731 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5209
AvaI C'yCGr_G
Cuts at: 1870 1943 2534 1870
Size: 73 591 4545
Fragments arranged by size:
4545 591 73
BamHI G'GATC_C
Cuts at: 2539 2906 2539
Size: 367 4842
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2302 2530 3985 618
Size: 325 217 627 515 228 1455 1842
Fragments arranged by size:
1842 1455 627 515 325 228 217
BanII G_rGCy'C
Cuts at: 1391 2075 2097 2274 2346 2528 1391
Size: 684 22 177 72 182 4072
Fragments arranged by size:
4072 684 182 177 72 22
BbeI G_GCGC'C
Cuts at: 2306 2306
Size: 5209
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2049 2446 928
Size: 971 150 397 3691
Fragments arranged by size:
3691 971 397 150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3400 3434 1903
Size: 34 1463 34 3678
Fragments arranged by size:
3678 1463 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3101 4311 3101
Size: 1210 3999
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5209
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3820 136
Size: 107 122 71 631 605 2148 1525
Fragments arranged by size:
2148 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5204 5204
Size: 5209
BlpI GC'TnA_GC
Cuts at: 2626 2626
Size: 5209
Bme1580I G_kGCm'C
Cuts at: 1790 2075 2097 2414 3270 4426 1790
Size: 285 22 317 856 1156 2573
Fragments arranged by size:
2573 1156 856 317 285 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5209
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2940 3898 442
Size: 416 53 83 399 24 1523 958 1753
Fragments arranged by size:
1753 1523 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2032 2422 3869 1239
Size: 793 390 1447 2579
Fragments arranged by size:
2579 1447 793 390
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3334 4199 4497 3334
Size: 865 298 4046
Fragments arranged by size:
4046 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3872 882
Size: 617 2373 2219
Fragments arranged by size:
2373 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3000 493
Size: 711 1796 2702
Fragments arranged by size:
2702 1796 711
BsaBI GATnn'nnATC
Cuts at: 5203 5203
Size: 5209
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2145 2303 3016 3398
Size: 53 83 186 936 612 158 713 382
Cuts at: 3398 4033 275
Size: 635 1451
Fragments arranged by size:
1451 936 713 635 612 382 186 158
83 53
BsaWI w'CCGG_w
Cuts at: 2211 2346 3642 4040 4314 4461 4872 2211
Size: 135 1296 398 274 147 411 2548
Fragments arranged by size:
2548 1296 411 398 274 147 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2341 2371 1178
Size: 30 165 30 938 30 4016
Fragments arranged by size:
4016 938 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 1186
Size: 1096 4113
BseYI C'CCAG_C
Cuts at: 2075 2195 2273 4412 4908 2075
Size: 120 78 2139 496 2376
Fragments arranged by size:
2376 2139 496 120 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4636
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2659 3420 4448 1540
Size: 275 186 658 761 1028 2301
Fragments arranged by size:
2301 1028 761 658 275 186
BsiHKAI G_wGCw'C
Cuts at: 2346 3270 3355 4426 2346
Size: 924 85 1071 3129
Fragments arranged by size:
3129 1071 924 85
BsmI GAATG_Cn'
Cuts at: 2720 2813 2720
Size: 93 5116
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2097 2274 2346 2414 2528 3270
Size: 399 285 22 177 72 68 114 742
Cuts at: 3270 3355 4426 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 285 177 114 85
72 68 22
BspEI T'CCGG_A
Cuts at: 2211 2346 4872 2211
Size: 135 2526 2548
Fragments arranged by size:
2548 2526 135
BspHI T'CATG_A
Cuts at: 3098 4828 3098
Size: 1730 3479
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2564 844
Size: 1720 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2292 3096 1394
Size: 429 469 804 3507
Fragments arranged by size:
3507 804 469 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3704 3878 65
Size: 1183 2456 174 1396
Fragments arranged by size:
2456 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3853 4040 1700
Size: 110 2043 187 2869
Fragments arranged by size:
2869 2043 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5209
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5209
BssSI C'ACGA_G
Cuts at: 3269 4281 3269
Size: 1012 4197
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5209
BstBI TT'CG_AA
Cuts at: 4013 4013
Size: 5209
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5209
BstYI r'GATC_y
Cuts at: 1972 2013 2208 2539 2906 3291 3308 4760 4846
Size: 41 195 331 367 385 17 1452 86
Cuts at: 4846 4858 5204 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 331 195 86
41 17 12
Bsu36I CC'TnA_GG
Cuts at: 2098 2098
Size: 5209
BtgI C'CryG_G
Cuts at: 513 1065 2235 513
Size: 552 1170 3487
Fragments arranged by size:
3487 1170 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2102 487
Size: 683 56 876 3594
Fragments arranged by size:
3594 876 683 56
BtsI GCAGTG_nn'
Cuts at: 2725 3519 3539 2725
Size: 794 20 4395
Fragments arranged by size:
4395 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5209
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5174
DraI TTT'AAA
Cuts at: 2513 2513
Size: 5209
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4216 809
Size: 507 2900 1802
Fragments arranged by size:
2900 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2335 2656 3545
Size: 54 1425 50 275 18 505 321 889
Cuts at: 3545 5174 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 505 321 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2656 1537
Size: 275 844 4090
Fragments arranged by size:
4090 844 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2396 3139 1353
Size: 1043 743 3423
Fragments arranged by size:
3423 1043 743
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2485 3796 4170 4316 387
Size: 1064 138 896 1311 374 146 1280
Fragments arranged by size:
1311 1280 1064 896 374 146 138
EcoICRI GAG'CTC
Cuts at: 2344 2344
Size: 5209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2422 3272 3869 4656 4924 1239
Size: 393 400 390 850 597 787 268 1524
Fragments arranged by size:
1524 850 787 597 400 393 390 268
EcoO109I rG'GnC_Cy
Cuts at: 2065 2072 2094 2125 2065
Size: 7 22 31 5149
Fragments arranged by size:
5149 31 22 7
EcoRI G'AATT_C
Cuts at: 2518 2896 2518
Size: 378 4831
FspI TGC'GCA
Cuts at: 3715 3715
Size: 5209
HaeII r_GCGC'y
Cuts at: 1678 2196 2306 2332 4356 1678
Size: 518 110 26 2024 2531
Fragments arranged by size:
2531 2024 518 110 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 2081 2113 2200
Size: 32 135 32 464 32 13 32 87
Cuts at: 2200 2232 3850 3882 3924 3956 1373
Size: 32 1618 32 42 32 2626
Fragments arranged by size:
2626 1618 464 135 87 42 32 32
32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2553 2799 669
Size: 988 281 615 246 3079
Fragments arranged by size:
3079 988 615 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5209
HpaI GTT'AAC
Cuts at: 2799 2799
Size: 5209
KasI G'GCGC_C
Cuts at: 2302 2302
Size: 5209
KpnI G_GTAC'C
Cuts at: 2534 2534
Size: 5209
MfeI C'AATT_G
Cuts at: 2808 2808
Size: 5209
MluI A'CGCG_T
Cuts at: 5085 5085
Size: 5209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4323 4507 1305
Size: 3018 184 2007
Fragments arranged by size:
3018 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3387
Fragments arranged by size:
3387 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3167 3526 3685 518
Size: 648 2001 359 159 2042
Fragments arranged by size:
2042 2001 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2237 2442 2625 3302 4450 4695 1606
Size: 631 205 183 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 631 245 205 183
NarI GG'CG_CC
Cuts at: 2303 2303
Size: 5209
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4657
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5209
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5209
NotI GC'GGCC_GC
Cuts at: 2656 2656
Size: 5209
NspI r_CATG'y
Cuts at: 1132 1229 2567 4112 1132
Size: 97 1338 1545 2229
Fragments arranged by size:
2229 1545 1338 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5209
PciI A'CATG_T
Cuts at: 4108 4108
Size: 5209
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2316 1108
Size: 1208 4001
PmeI GTTT'AAAC
Cuts at: 2513 2513
Size: 5209
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 4791 4823 3278
Size: 32 1481 32 3664
Fragments arranged by size:
3664 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2148 2450 1613
Size: 535 302 4372
Fragments arranged by size:
4372 535 302
PsiI TTA'TAA
Cuts at: 2779 2779
Size: 5209
PspOMI G'GGCC_C
Cuts at: 2071 2093 2071
Size: 22 5187
PstI C_TGCA'G
Cuts at: 830 2059 2561 830
Size: 1229 502 3478
Fragments arranged by size:
3478 1229 502
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5209
PvuII CAG'CTG
Cuts at: 2442 2442
Size: 5209
SacI G_AGCT'C
Cuts at: 2346 2346
Size: 5209
SacII CC_GC'GG
Cuts at: 2238 2238
Size: 5209
SalI G'TCGA_C
Cuts at: 1936 2551 1936
Size: 615 4594
SapI GCTCTTCn'nnn_
Cuts at: 2396 2396
Size: 5209
SbfI CC_TGCA'GG
Cuts at: 2561 2561
Size: 5209
ScaI AGT'ACT
Cuts at: 3457 5169 3457
Size: 1712 3497
SfcI C'TryA_G
Cuts at: 826 1045 2055 2557 3692 4373 4564 826
Size: 219 1010 502 1135 681 191 1471
Fragments arranged by size:
1471 1135 1010 681 502 219 191
SfoI GGC'GCC
Cuts at: 2304 2304
Size: 5209
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5209
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5209
SmaI CCC'GGG
Cuts at: 1872 2536 1872
Size: 664 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3313 4214 4476 820
Size: 197 926 1370 901 262 1553
Fragments arranged by size:
1553 1370 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3000 493
Size: 2507 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5209
SphI G_CATG'C
Cuts at: 1132 2567 1132
Size: 1435 3774
SspI AAT'ATT
Cuts at: 5 52 3133 5
Size: 47 3081 2081
Fragments arranged by size:
3081 2081 47
StuI AGG'CCT
Cuts at: 1517 2133 1517
Size: 616 4593
StyI C'CwwG_G
Cuts at: 513 1065 2648 513
Size: 552 1583 3074
Fragments arranged by size:
3074 1583 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3236 3253 3406 3591 4036 325
Size: 1255 1656 17 153 185 445 1498
Fragments arranged by size:
1656 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3455 5167 96
Size: 274 80 33 51 1443 1478 1712 138
Fragments arranged by size:
1712 1478 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2341 2373 5169 5201 2341
Size: 32 2796 32 2349
Fragments arranged by size:
2796 2349 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5209
XbaI T'CTAG_A
Cuts at: 2545 2545
Size: 5209
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2136 2136
Size: 5209
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5209
XmaI C'CCGG_G
Cuts at: 1870 2534 1870
Size: 664 4545
XmnI GAAnn'nnTTC
Cuts at: 3338 3338
Size: 5209
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3017 276
Size: 53 83 186 936 1483 2468
Fragments arranged by size:
2468 1483 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI
SfiI SrfI SwaI