Restriction map for
pFN21AB8229
(Linear) (Six-base) MAPSORT of: KIBB8229.seq Check: 1013 from: 1 to: 504
KIBB8229.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AfeI AGC'GCT
Cuts at: 0 325 504
Size: 325 179
ApaI G_GGCC'C
Cuts at: 0 70 92 504
Size: 70 22 412
Fragments arranged by size:
412 70 22
BanI G'GyrC_C
Cuts at: 0 297 504
Size: 297 207
BanII G_rGCy'C
Cuts at: 0 70 92 269 341 504
Size: 70 22 177 72 163
Fragments arranged by size:
177 163 72 70 22
BbeI G_GCGC'C
Cuts at: 0 301 504
Size: 301 203
BbsI GAAGACnn'nnnn_
Cuts at: 0 44 441 504
Size: 44 397 63
Fragments arranged by size:
397 63 44
Bme1580I G_kGCm'C
Cuts at: 0 70 92 409 504
Size: 70 22 317 95
Fragments arranged by size:
317 95 70 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 417 504
Size: 27 390 87
Fragments arranged by size:
390 87 27
BsaHI Gr'CG_yC
Cuts at: 0 140 298 504
Size: 140 158 206
Fragments arranged by size:
206 158 140
BsaWI w'CCGG_w
Cuts at: 0 206 341 504
Size: 206 135 163
Fragments arranged by size:
206 163 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 336 366 504
Size: 336 30 138
Fragments arranged by size:
336 138 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 504
Size: 277 227
BseYI C'CCAG_C
Cuts at: 0 70 190 268 504
Size: 70 120 78 236
Fragments arranged by size:
236 120 78 70
BsiHKAI G_wGCw'C
Cuts at: 0 341 504
Size: 341 163
Bsp1286I G_dGCh'C
Cuts at: 0 70 92 269 341 409 504
Size: 70 22 177 72 68 95
Fragments arranged by size:
177 95 72 70 68 22
BspEI T'CCGG_A
Cuts at: 0 206 341 504
Size: 206 135 163
Fragments arranged by size:
206 163 135
BsrBI CCG'CTC
Cuts at: 0 287 504
Size: 287 217
BstYI r'GATC_y
Cuts at: 0 8 203 504
Size: 8 195 301
Fragments arranged by size:
301 195 8
Bsu36I CC'TnA_GG
Cuts at: 0 93 504
Size: 93 411
BtgI C'CryG_G
Cuts at: 0 230 504
Size: 230 274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 97 504
Size: 97 407
EaeI y'GGCC_r
Cuts at: 0 330 504
Size: 330 174
EarI CTCTTCn'nnn_
Cuts at: 0 391 504
Size: 391 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 480 504
Size: 480 24
EcoICRI GAG'CTC
Cuts at: 0 339 504
Size: 339 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 417 504
Size: 27 390 87
Fragments arranged by size:
390 87 27
EcoO109I rG'GnC_Cy
Cuts at: 0 60 67 89 120 504
Size: 60 7 22 31 384
Fragments arranged by size:
384 60 31 22 7
HaeII r_GCGC'y
Cuts at: 0 191 301 327 504
Size: 191 110 26 177
Fragments arranged by size:
191 177 110 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 76 108 195 227 504
Size: 31 32 13 32 87 32 277
Fragments arranged by size:
277 87 32 32 32 31 13
KasI G'GCGC_C
Cuts at: 0 297 504
Size: 297 207
MspA1I CmG'CkG
Cuts at: 0 232 437 504
Size: 232 205 67
Fragments arranged by size:
232 205 67
NarI GG'CG_CC
Cuts at: 0 298 504
Size: 298 206
PflMI CCAn_nnn'nTGG
Cuts at: 0 311 504
Size: 311 193
PshAI GACnn'nnGTC
Cuts at: 0 143 445 504
Size: 143 302 59
Fragments arranged by size:
302 143 59
PspOMI G'GGCC_C
Cuts at: 0 66 88 504
Size: 66 22 416
Fragments arranged by size:
416 66 22
PstI C_TGCA'G
Cuts at: 0 54 504
Size: 54 450
PvuII CAG'CTG
Cuts at: 0 437 504
Size: 437 67
SacI G_AGCT'C
Cuts at: 0 341 504
Size: 341 163
SacII CC_GC'GG
Cuts at: 0 233 504
Size: 233 271
SapI GCTCTTCn'nnn_
Cuts at: 0 391 504
Size: 391 113
SfcI C'TryA_G
Cuts at: 0 50 504
Size: 50 454
SfoI GGC'GCC
Cuts at: 0 299 504
Size: 299 205
StuI AGG'CCT
Cuts at: 0 128 504
Size: 128 376
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 336 368 504
Size: 336 32 136
Fragments arranged by size:
336 136 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 131 504
Size: 131 373
Enzymes that do cut:
AfeI ApaI BanI BanII BbeI BbsI Bme1580I BpmI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspEI
BsrBI BstYI Bsu36I BtgI BtgZI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MspA1I NarI
PflMI PshAI PspOMI PstI PvuII SacI SacII SapI
SfcI SfoI StuI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsgI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII
TatI Tth111I XbaI XhoI XmaI XmnI ZraI