Restriction map for pFN21AB8227
 (Circular) (Six-base) MAPSORT of: pFN21AB8227.seq  Check: 3179  from: 1  to: 5260



pFN21AB8227  5260 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:41  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3070     278 
   Size:         53      83     186     936    1534    2468

  Fragments arranged by size: 

                2468    1534     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2603    1937 
   Size:        666    4594
 
Acc65I G'GTAC_C

Cuts at:   2581    2581 
   Size:       5260
 
AclI AA'CG_TT

Cuts at:   1563    3387    3760    1563 
   Size:       1824     373    3063

  Fragments arranged by size: 

                3063    1824     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3323    4707    4975    1632 
   Size:       1691    1384     268    1917

  Fragments arranged by size: 

                1917    1691    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5063
 
AflIII A'CryG_T

Cuts at:   1203    4159    5136    1203 
   Size:       2956     977    1327

  Fragments arranged by size: 

                2956    1327     977
 
AgeI A'CCGG_T

Cuts at:   4091    4091 
   Size:       5260
 
AhdI GACnn_n'nnGTC

Cuts at:   3989    3989 
   Size:       5260
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5260
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5260
 
AlwNI CAG_nnn'CTG

Cuts at:   2282    3003    4575    2282 
   Size:        721    1572    2967

  Fragments arranged by size: 

                2967    1572     721
 
ApaLI G'TGCA_C

Cuts at:   3317    4473    3317 
   Size:       1156    4104
 
ApoI r'AATT_y

Cuts at:   1493    2569    2795    2947    5056    1493 
   Size:       1076     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1076     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3814     160 
   Size:        872    2782    1606

  Fragments arranged by size: 

                2782    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5260
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2387    2585    1870 
   Size:         73     444     198    4545

  Fragments arranged by size: 

                4545     444     198      73
 
BamHI G'GATC_C

Cuts at:   2590    2957    2590 
   Size:        367    4893
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2581    4036     618 
   Size:        325     217     627     794    1455    1842

  Fragments arranged by size: 

                1842    1455     794     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2579    1391 
   Size:       1188    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4289
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3451    3485    1903 
   Size:         34    1514      34    3678

  Fragments arranged by size: 

                3678    1514      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3152    4362    3152 
   Size:       1210    4050
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5260
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2015    3871     136 
   Size:        107     122      71     631     605     343    1856    1525

  Fragments arranged by size: 

                1856    1525     631     605     343     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5255    5255 
   Size:       5260
 
BlpI GC'TnA_GC

Cuts at:   2677    2677 
   Size:       5260
 
Bme1580I G_kGCm'C

Cuts at:   1790    3321    4477    1790 
   Size:       1531    1156    2573

  Fragments arranged by size: 

                2573    1531    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5260
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2139    2991    3949 
   Size:        416      53      83     399      24     722     852     958

Cuts at:   3949     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     852     722     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5260
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2442    3920    1239 
   Size:       1203    1478    2579

  Fragments arranged by size: 

                2579    1478    1203
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3385    4250    4548    3385 
   Size:        865     298    4097

  Fragments arranged by size: 

                4097     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3923     882 
   Size:        617    2424    2219

  Fragments arranged by size: 

                2424    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3051     493 
   Size:        711    1847    2702

  Fragments arranged by size: 

                2702    1847     711
 
BsaBI GATnn'nnATC

Cuts at:   5254    5254 
   Size:       5260
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2370    3067    3449    4084 
   Size:         53      83     186     936     837     697     382     635

Cuts at:   4084     275 
   Size:       1451

  Fragments arranged by size: 

                1451     936     837     697     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2264    2511    3693    4091    4365    4512    4923    2264 
   Size:        247    1182     398     274     147     411    2601

  Fragments arranged by size: 

                2601    1182     411     398     274     247     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2163    2193    1178 
   Size:         30     165      30     760      30    4245

  Fragments arranged by size: 

                4245     760     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5260
 
BseYI C'CCAG_C

Cuts at:   2047    2150    4463    4959    2047 
   Size:        103    2313     496    2348

  Fragments arranged by size: 

                2348    2313     496     103
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4687
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2710    3471    4499    1540 
   Size:        275     186     709     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     709     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3321    3406    4477    3321 
   Size:         85    1071    4104

  Fragments arranged by size: 

                4104    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2521    2771    2864    2521 
   Size:        250      93    4917

  Fragments arranged by size: 

                4917     250      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2579    3321    3406    4477    1391 
   Size:        399     789     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     789     742     399      85
 
BspEI T'CCGG_A

Cuts at:   4923    4923 
   Size:       5260
 
BspHI T'CATG_A

Cuts at:   3149    4879    3149 
   Size:       1730    3530
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2615     844 
   Size:       1771    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3147    1394 
   Size:        429    1324    3507

  Fragments arranged by size: 

                3507    1324     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2439    3755    3929      65 
   Size:       1183    1191    1316     174    1396

  Fragments arranged by size: 

                1396    1316    1191    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3904    4091    1700 
   Size:        110    2094     187    2869

  Fragments arranged by size: 

                2869    2094     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5260
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5260
 
BssSI C'ACGA_G

Cuts at:   3320    4332    3320 
   Size:       1012    4248
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5260
 
BstBI TT'CG_AA

Cuts at:   4064    4064 
   Size:       5260
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5260
 
BstYI r'GATC_y

Cuts at:   1972    2590    2957    3342    3359    4811    4897    4909    5255 
   Size:        618     367     385      17    1452      86      12     346

Cuts at:   5255    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     618     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2011    2415     513 
   Size:        552     939       7     404    3358

  Fragments arranged by size: 

                3358     939     552     404       7
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4521

  Fragments arranged by size: 

                4521     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2124    2332    2776    3570    3590    2124 
   Size:        208     444     794      20    3794

  Fragments arranged by size: 

                3794     794     444     208      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5260
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5225
 
DraI TTT'AAA

Cuts at:   2564    2564 
   Size:       5260
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4267     809 
   Size:        507    2951    1802

  Fragments arranged by size: 

                2951    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2707    3596    5225 
   Size:         54    1425      50     275      18     877     889    1629

Cuts at:   5225       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     877     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2707    1537 
   Size:        275     895    4090

  Fragments arranged by size: 

                4090     895     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2042    2355    3190    1353 
   Size:        689     313     835    3423

  Fragments arranged by size: 

                3423     835     689     313
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3847    4221    4367     387 
   Size:       1064     138    2258     374     146    1280

  Fragments arranged by size: 

                2258    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2442    3323    3920    4707    4975    1239 
   Size:        393     810     881     597     787     268    1524

  Fragments arranged by size: 

                1524     881     810     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2569    2947    2569 
   Size:        378    4882
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2482    2482 
   Size:       5260
 
FspI TGC'GCA

Cuts at:   3766    3766 
   Size:       5260
 
HaeII r_GCGC'y

Cuts at:   1678    2030    4407    1678 
   Size:        352    2377    2531

  Fragments arranged by size: 

                2531    2377     352
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2221    2253    3901    3933    3975 
   Size:         32     135      32     649      32    1648      32      42

Cuts at:   3975    4007    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1648     649     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2604    2850     669 
   Size:        988     281     666     246    3079

  Fragments arranged by size: 

                3079     988     666     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5260
 
HpaI GTT'AAC

Cuts at:   2850    2850 
   Size:       5260
 
KpnI G_GTAC'C

Cuts at:   2585    2585 
   Size:       5260
 
MfeI C'AATT_G

Cuts at:   2859    2859 
   Size:       5260
 
MluI A'CGCG_T

Cuts at:   5136    5136 
   Size:       5260
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4374    4558    1305 
   Size:       3069     184    2007

  Fragments arranged by size: 

                3069    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3438

  Fragments arranged by size: 

                3438    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2514    3218    3577    3736     518 
   Size:        648    1348     704     359     159    2042

  Fragments arranged by size: 

                2042    1348     704     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2013    2534    2676    3353    4501    4746    1606 
   Size:        407     521     142     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     521     407     245     142
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3769

  Fragments arranged by size: 

                3769     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5260
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5260
 
NotI GC'GGCC_GC

Cuts at:   2707    2707 
   Size:       5260
 
NspI r_CATG'y

Cuts at:   1132    1229    2618    4163    1132 
   Size:         97    1389    1545    2229

  Fragments arranged by size: 

                2229    1545    1389      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5260
 
PciI A'CATG_T

Cuts at:   4159    4159 
   Size:       5260
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5260
 
PmeI GTTT'AAAC

Cuts at:   2564    2564 
   Size:       5260
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3329    3361    4842    4874    3329 
   Size:         32    1481      32    3715

  Fragments arranged by size: 

                3715    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5260
 
PsiI TTA'TAA

Cuts at:   2830    2830 
   Size:       5260
 
PstI C_TGCA'G

Cuts at:    830    2612     830 
   Size:       1782    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5260
 
PvuII CAG'CTG

Cuts at:   2534    2534 
   Size:       5260
 
SacII CC_GC'GG

Cuts at:   2014    2014 
   Size:       5260
 
SalI G'TCGA_C

Cuts at:   1936    2602    1936 
   Size:        666    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2042    2042 
   Size:       5260
 
SbfI CC_TGCA'GG

Cuts at:   2612    2612 
   Size:       5260
 
ScaI AGT'ACT

Cuts at:   3508    5220    3508 
   Size:       1712    3548
 
SfcI C'TryA_G

Cuts at:    826    1045    2608    3743    4424    4615     826 
   Size:        219    1563    1135     681     191    1471

  Fragments arranged by size: 

                1563    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5260
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5260
 
SmaI CCC'GGG

Cuts at:   1872    2389    2587    1872 
   Size:        517     198    4545

  Fragments arranged by size: 

                4545     517     198
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3364    4265    4527     820 
   Size:        197     926    1421     901     262    1553

  Fragments arranged by size: 

                1553    1421     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3051     493 
   Size:       2558    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5260
 
SphI G_CATG'C

Cuts at:   1132    2618    1132 
   Size:       1486    3774
 
SspI AAT'ATT

Cuts at:      5      52    3184       5 
   Size:         47    3132    2081

  Fragments arranged by size: 

                3132    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5260
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2699     513 
   Size:        552     939     695    3074

  Fragments arranged by size: 

                3074     939     695     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2039    2236    3287    3304    3457    3642    4087 
   Size:       1255     459     197    1051      17     153     185     445

Cuts at:   4087     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1051     459     445     197     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3506    5218      96 
   Size:        274      80      33      51    1443    1529    1712     138

  Fragments arranged by size: 

                1712    1529    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2243    2275    5220    5252    2243 
   Size:         32    2945      32    2251

  Fragments arranged by size: 

                2945    2251      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5260
 
XbaI T'CTAG_A

Cuts at:   2596    2596 
   Size:       5260
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5260
 
XmaI C'CCGG_G

Cuts at:   1870    2387    2585    1870 
   Size:        517     198    4545

  Fragments arranged by size: 

                4545     517     198
 
XmnI GAAnn'nnTTC

Cuts at:   3389    3389 
   Size:       5260
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3068     276 
   Size:         53      83     186     936    1534    2468

  Fragments arranged by size: 

                2468    1534     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI    PvuII    SacII     SalI     SapI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    RsrII     SacI    SanDI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com