Restriction map for
pFN21AB8208
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# NO CUT ENZYMES ( 198 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DpnII DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I TseI Tsp45I TspRI
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AcsI 151 AAATTT [GA]^AATT[CT]
AfaI 325 GTAC GT^AC
Alw44I 122 GTGCAC G^TGCAC
ApaLI 122 GTGCAC G^TGCAC
ApoI 151 AAATTT [GA]^AATT[CT]
AspHI 122 GTGCAC G[AT]GC[AT]^C
AviII 185 TGCGCA TGC^GCA
BpmI 176 CTCCAG CTGGAG(16/14)
BsiHKAI 122 GTGCAC G[AT]GC[AT]^C
BsiYI 99 CCATGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 99 CCATGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BssKI 197 CCAGG ^CC[ACGT]GG
BstNI 197 CCAGG CC^[AT]GG
Cac8I 120 GCGTGC GC[ACGT]^[ACGT]GC
DsaI 99 CCATGG C^C[GA][CT]GG
EcoRII 197 CCAGG ^CC[AT]GG
EcoT14I 99 CCATGG C^C[AT][AT]GG
FauI 7 CCCGC CCCGC(4/6)
Fnu4HI 258 GCGGC GC^[ACGT]GC
FspI 185 TGCGCA TGC^GCA
HphI 292 TCACC GGTGA(8/7)
ItaI 258 GCGGC GC^[ACGT]GC
MaeII 273 ACGT A^CGT
MvaI 197 CCAGG CC^[AT]GG
NcoI 99 CCATGG C^CATGG
NlaIV 3 GGCTCC GG[ACGT]^[ACGT]CC
PflMI 99 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 325 GTAC GT^AC
ScrFI 197 CCAGG CC^[ACGT]GG
SfaNI 95 GCATC GCATC(5/9)
SfcI 349 CTACAG C^T[GA][CT]AG
StyI 99 CCATGG C^C[AT][AT]GG
Tsp509I 152 AATT ^AATT
Van91I 99 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 8 CCGC CCGC(-3/-1)
258 GCGG
AluI 210 AGCT AG^CT
302 AGCT
AvaI 65 CCCGAG C^[CT]CG[GA]G
128 CTCGGG
BmyI 122 GTGCAC G[AGT]GC[ACT]^C
131 GGGCAC
BsaJI 99 CCATGG C^C[ACGT][ACGT]GG
127 CCTCGG
BsoBI 65 CCCGAG C^[CT]CG[GA]G
128 CTCGGG
Bsp1286I 122 GTGCAC G[AGT]GC[ACT]^C
131 GGGCAC
BstEII 60 GGTAACC G^GT[ACGT]ACC
315 GGTTACC
BstPI 60 GGTAACC G^GT[ACGT]ACC
315 GGTTACC
CfoI 93 GCGC GCG^C
186 GCGC
Cfr13I 36 GGGCC G^G[ACGT]CC
218 GGGCC
EcoO65I 60 GGTAACC G^GT[ACGT]ACC
315 GGTTACC
HaeIII 37 GGCC GG^CC
219 GGCC
HhaI 93 GCGC GCG^C
186 GCGC
HinP1I 93 GCGC G^CGC
186 GCGC
MboII 27 GAAGA GAAGA(8/7)
286 GAAGA
MnlI 127 CCTC CCTC(7/6)
280 GAGG
MwoI 124 GCACCTCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
211 GCTGTCTGGGC
NlaIII 100 CATG CATG^
159 CATG
Sau96I 36 GGGCC G^G[ACGT]CC
218 GGGCC
XcmI 47 CCATCAACGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
158 CCATGAAGGAGATGG
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# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
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BstF5I FokI HinfI MaeIII TfiI