Restriction map for
pFN21AB8207
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AccI AccII AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspHI AspI AviII AvrII BamHI
BanII BbeI BbrPI BbsI BciVI
BclI BfaI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KspI
MaeI MamI MfeI MluI MroI
MseI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tth111I
Van91I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 202 GACGTC GACGT^C
Acc65I 497 GGTACC G^GTACC
AcsI 241 GAATTC [GA]^AATT[CT]
AflIII 551 ACATGT A^C[GA][CT]GT
AhdI 550 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 241 GAATTC [GA]^AATT[CT]
Asp718I 497 GGTACC G^GTACC
AspEI 550 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 443 CTCGAG C^[CT]CG[GA]G
BalI 584 TGGCCA TGG^CCA
BbvCI 478 GCTGAGG CCTCAGC(-5/-2)
BcgI 614 GCAATGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 152 AGATCT A^GATCT
BmyI 754 GTGCCC G[AGT]GC[ACT]^C
BsaWI 507 ACCGGA [AT]^CCGG[AT]
BseRI 670 GAGGAG GAGGAG(10/8)
BsgI 208 CTGCAC GTGCAG(16/14)
BsmAI 73 GAGAC GTCTC(1/5)
BsoBI 443 CTCGAG C^[CT]CG[GA]G
Bsp1286I 754 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 551 ACATGT A^CATGT
BspMI 392 ACCTGC ACCTGC(4/8)
BsrFI 562 GCCGGC [GA]^CCGG[CT]
BstEII 286 GGTGACC G^GT[ACGT]ACC
BstPI 286 GGTGACC G^GT[ACGT]ACC
Cfr10I 562 GCCGGC [GA]^CCGG[CT]
DraIII 489 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 550 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 159 CCTACTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 286 GGTGACC G^GT[ACGT]ACC
EcoRI 241 GAATTC G^AATTC
HgaI 660 GACGC GACGC(5/10)
KpnI 497 GGTACC GGTAC^C
MluNI 584 TGGCCA TGG^CCA
MscI 584 TGGCCA TGG^CCA
MslI 546 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 562 GCCGGC GCC^GGC
NgoMIV 562 GCCGGC G^CCGGC
NspI 551 ACATGT [GA]CATG^[CT]
PaeR7I 443 CTCGAG C^TCGAG
PleI 75 GACTC GAGTC(4/5)
PshAI 862 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 476 CAGCTG CAG^CTG
SfaNI 569 GCATC GCATC(5/9)
XcmI 731 CCACAACATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 443 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 202 GACGTC G[GA]^CG[CT]C
660 GACGCC
AlwNI 253 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
388 CAGAACCTG
AvaII 255 GGACC G^G[AT]CC
375 GGACC
BanI 37 GGCACC G^G[CT][GA]CC
497 GGTACC
BsaHI 202 GACGTC G[GA]^CG[CT]C
660 GACGCC
BsaJI 668 CCGAGG C^C[ACGT][ACGT]GG
740 CCCTGG
BsmFI 374 GGGAC GGGAC(10/14)
658 GGGAC
BspHI 78 TCATGA T^CATGA
198 TCATGA
BsrI 18 ACTGG ACTGG(1/-1)
630 CCAGT
Cac8I 562 GCCGGC GC[ACGT]^[ACGT]GC
582 GCTGGC
EaeI 584 TGGCCA [CT]^GGCC[GA]
728 CGGCCA
Hin1I 202 GACGTC G[GA]^CG[CT]C
660 GACGCC
MspA1I 465 CCGCTG C[AC]G^C[GT]G
476 CAGCTG
PpuMI 254 AGGACCT [GA]G^G[AT]CC[CT]
374 GGGACCT
PstI 787 CTGCAG CTGCA^G
841 CTGCAG
RcaI 78 TCATGA T^CATGA
198 TCATGA
SfcI 787 CTGCAG C^T[GA][CT]AG
841 CTGCAG
SmlI 163 CTCAAG C^T[CT][GA]AG
443 CTCGAG
TfiI 344 GATTC G^A[AT]TC
456 GATTC
Tsp509I 242 AATT ^AATT
422 AATT
TspRI 491 CACTG CA[GC]TG[ACGT][ACGT]^
676 CAGTG
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BsiYI BslI BsrDI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TaqI TseI Tsp45I TthHB8I
XhoII