Restriction map for pFN21AB8207
 (Circular) (Six-base) MAPSORT of: pFN21AB8207.seq  Check: 362  from: 1  to: 5581



pFN21AB8207  5581 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:41  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2211    3391     278 
   Size:         53      83     186     936     675    1180    2468

  Fragments arranged by size: 

                2468    1180     936     675     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2924    1937 
   Size:        987    4594
 
Acc65I G'GTAC_C

Cuts at:   2502    2902    2502 
   Size:        400    5181
 
AclI AA'CG_TT

Cuts at:   1563    3708    4081    1563 
   Size:       2145     373    3063

  Fragments arranged by size: 

                3063    2145     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2198    2285    2405    2825    3644    5028    5296    1632 
   Size:        566      87     120     420     819    1384     268    1917

  Fragments arranged by size: 

                1917    1384     819     566     420     268     120      87
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5384
 
AflIII A'CryG_T

Cuts at:   1203    2556    4480    5457    1203 
   Size:       1353    1924     977    1327

  Fragments arranged by size: 

                1924    1353    1327     977
 
AgeI A'CCGG_T

Cuts at:   4412    4412 
   Size:       5581
 
AhdI GACnn_n'nnGTC

Cuts at:   2560    4310    2560 
   Size:       1750    3831
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5581
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5581
 
AlwNI CAG_nnn'CTG

Cuts at:   2263    2398    3324    4896    2263 
   Size:        135     926    1572    2948

  Fragments arranged by size: 

                2948    1572     926     135
 
ApaLI G'TGCA_C

Cuts at:   3638    4794    3638 
   Size:       1156    4425
 
ApoI r'AATT_y

Cuts at:   1493    2246    2890    3116    3268    5377    1493 
   Size:        753     644     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     753     644     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4135     160 
   Size:        872    3103    1606

  Fragments arranged by size: 

                3103    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5581
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2448    2906    1870 
   Size:         73     505     458    4545

  Fragments arranged by size: 

                4545     505     458      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2486    2519    2486 
   Size:         33    5548
 
BamHI G'GATC_C

Cuts at:   2911    3278    2911 
   Size:        367    5214
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2042    2502    2902    4357     618 
   Size:        325     217     627     255     460     400    1455    1842

  Fragments arranged by size: 

                1842    1455     627     460     400     325     255     217
            
 
BanII G_rGCy'C

Cuts at:   1391    2900    1391 
   Size:       1509    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4610
 
BbvCI CC'TCA_GC

Cuts at:   2484    2484 
   Size:       5581
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    2608    2642    3772    3806    1903 
   Size:         34      50      34     587      34    1130      34    3678

  Fragments arranged by size: 

                3678    1130     587      50      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   3473    4683    3473 
   Size:       1210    4371
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5581
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4192     136 
   Size:        107     122      71     631     605    2520    1525

  Fragments arranged by size: 

                2520    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2157    5576    2157 
   Size:       3419    2162
 
BlpI GC'TnA_GC

Cuts at:   2998    2998 
   Size:       5581
 
Bme1580I G_kGCm'C

Cuts at:   1790    2763    3642    4798    1790 
   Size:        973     879    1156    2573

  Fragments arranged by size: 

                2573    1156     973     879
 
BmgBI CAC'GTC

Cuts at:   1318    2547    1318 
   Size:       1229    4352
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3312    4270     442 
   Size:        416      53      83     399      24    1895     958    1753

  Fragments arranged by size: 

                1895    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5581
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4241    1239 
   Size:       3002    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2484    2484 
   Size:       5581
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2153    3706    4571    4869    2153 
   Size:       1553     865     298    2865

  Fragments arranged by size: 

                2865    1553     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4244     882 
   Size:        617    2745    2219

  Fragments arranged by size: 

                2745    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3372     493 
   Size:        711    2168    2702

  Fragments arranged by size: 

                2702    2168     711
 
BsaBI GATnn'nnATC

Cuts at:   5575    5575 
   Size:       5581
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2208    2666    3388    3770 
   Size:         53      83     186     936     675     458     722     382

Cuts at:   3770    4405     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451     936     722     675     635     458     382     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   2512    4014    4412    4686    4833    5244    2512 
   Size:       1502     398     274     147     411    2849

  Fragments arranged by size: 

                2849    1502     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5356

  Fragments arranged by size: 

                5356     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2690    1186 
   Size:       1504    4077
 
BseYI C'CCAG_C

Cuts at:   2344    2597    2659    4784    5280    2344 
   Size:        253      62    2125     496    2645

  Fragments arranged by size: 

                2645    2125     496     253      62
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2198    1163 
   Size:        573     462    4546

  Fragments arranged by size: 

                4546     573     462
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3031    3792    4820    1540 
   Size:        275     186    1030     761    1028    2301

  Fragments arranged by size: 

                2301    1030    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3642    3727    4798    3642 
   Size:         85    1071    4425

  Fragments arranged by size: 

                4425    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3092    3185    3092 
   Size:         93    5488
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2763    2900    3642    3727    4798    1391 
   Size:        399     973     137     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     973     742     399     137      85
 
BspEI T'CCGG_A

Cuts at:   5244    5244 
   Size:       5581
 
BspHI T'CATG_A

Cuts at:   2083    2203    3470    5200    2083 
   Size:        120    1267    1730    2464

  Fragments arranged by size: 

                2464    1730    1267     120
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2406    2936     844 
   Size:       1562     530    3489

  Fragments arranged by size: 

                3489    1562     530
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3468    1394 
   Size:        429    1645    3507

  Fragments arranged by size: 

                3507    1645     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2293    2371    2626    4076    4250      65 
   Size:       1183    1045      78     255    1450     174    1396

  Fragments arranged by size: 

                1450    1396    1183    1045     255     174      78
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2567    4225    4412    1700 
   Size:        110     757    1658     187    2869

  Fragments arranged by size: 

                2869    1658     757     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5581
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5581
 
BssSI C'ACGA_G

Cuts at:   3641    4653    3641 
   Size:       1012    4569
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5581
 
BstBI TT'CG_AA

Cuts at:   4385    4385 
   Size:       5581
 
BstEII G'GTnAC_C

Cuts at:   1184    2291    1184 
   Size:       1107    4474
 
BstYI r'GATC_y

Cuts at:   1972    2157    2646    2785    2911    3278    3663    3680    5132 
   Size:        185     489     139     126     367     385      17    1452

Cuts at:   5132    5218    5230    5576    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     489     385     367     346     185     139
                 126      86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5029
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4842

  Fragments arranged by size: 

                4842     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2687    3097    3891    3911    2687 
   Size:        410     794      20    4357

  Fragments arranged by size: 

                4357     794     410      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5581
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5546
 
DraI TTT'AAA

Cuts at:   2885    2885 
   Size:       5581
 
DraIII CAC_nnn'GTG

Cuts at:   2499    2499 
   Size:       5581
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4588     809 
   Size:        507    3272    1802

  Fragments arranged by size: 

                3272    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2589    2733    3028 
   Size:         54    1425      50     275      18     759     144     295

Cuts at:   3028    3917    5546       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     759     295     275     144      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3028    1537 
   Size:        275    1216    4090

  Fragments arranged by size: 

                4090    1216     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2330    2636    2832    3511    1353 
   Size:        977     306     196     679    3423

  Fragments arranged by size: 

                3423     977     679     306     196
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2134    2865    4168    4542    4688     387 
   Size:       1064     138     545     731    1303     374     146    1280

  Fragments arranged by size: 

                1303    1280    1064     731     545     374     146     138
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2198    2285    2405    2825    3644    4241    5028 
   Size:        393     566      87     120     420     819     597     787

Cuts at:   5028    5296    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     819     787     597     566     420     393     268
                 120      87
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2168    2168 
   Size:       5581
 
EcoO109I rG'GnC_Cy

Cuts at:   2260    2380    2842    2260 
   Size:        120     462    4999

  Fragments arranged by size: 

                4999     462     120
 
EcoRI G'AATT_C

Cuts at:   2246    2890    3268    2246 
   Size:        644     378    4559

  Fragments arranged by size: 

                4559     644     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2665    2665 
   Size:       5581
 
FspI TGC'GCA

Cuts at:   4087    4087 
   Size:       5581
 
HaeII r_GCGC'y

Cuts at:   1678    4728    1678 
   Size:       3050    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2080    2112    2546    2578    4222 
   Size:         32     135      32     508      32     434      32    1644

Cuts at:   4222    4254    4296    4328    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1644     508     434     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2925    3171     669 
   Size:        988     281     987     246    3079

  Fragments arranged by size: 

                3079     988     987     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5581
 
HpaI GTT'AAC

Cuts at:   3171    3171 
   Size:       5581
 
KpnI G_GTAC'C

Cuts at:   2506    2906    2506 
   Size:        400    5181
 
MfeI C'AATT_G

Cuts at:   3180    3180 
   Size:       5581
 
MluI A'CGCG_T

Cuts at:   5457    5457 
   Size:       5581
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2677    2889    4695    4879    1305 
   Size:       1372     212    1806     184    2007

  Fragments arranged by size: 

                2007    1806    1372     212     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2591      10 
   Size:         54    1425     343     759    3000

  Fragments arranged by size: 

                3000    1425     759     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2555    3539    3898    4057     518 
   Size:        648    1389     984     359     159    2042

  Fragments arranged by size: 

                2042    1389     984     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2472    2483    2997    3674    4822    5067    1606 
   Size:        866      11     514     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     866     677     514     245      11
 
NaeI GCC'GGC

Cuts at:   2569    2569 
   Size:       5581
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5029
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5581
 
NgoMIV G'CCGG_C

Cuts at:   2567    2567 
   Size:       5581
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5581
 
NotI GC'GGCC_GC

Cuts at:   3028    3028 
   Size:       5581
 
NspI r_CATG'y

Cuts at:   1132    1229    2560    2939    4484    1132 
   Size:         97    1331     379    1545    2229

  Fragments arranged by size: 

                2229    1545    1331     379      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5581
 
PciI A'CATG_T

Cuts at:   2556    4480    2556 
   Size:       1924    3657
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5581
 
PfoI T'CCnGG_A

Cuts at:   2151    2151 
   Size:       5581
 
PmeI GTTT'AAAC

Cuts at:   2885    2885 
   Size:       5581
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3650    3682    5163    5195    3650 
   Size:         32    1481      32    4036

  Fragments arranged by size: 

                4036    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2260    2380    2260 
   Size:        120    5461
 
PshAI GACnn'nnGTC

Cuts at:   1613    2871    1613 
   Size:       1258    4323
 
PsiI TTA'TAA

Cuts at:   3151    3151 
   Size:       5581
 
PspXI vC'TCGA_Gb

Cuts at:   2448    2448 
   Size:       5581
 
PstI C_TGCA'G

Cuts at:    830    2796    2850    2933     830 
   Size:       1966      54      83    3478

  Fragments arranged by size: 

                3478    1966      83      54
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5581
 
PvuII CAG'CTG

Cuts at:   2483    2483 
   Size:       5581
 
SalI G'TCGA_C

Cuts at:   1936    2923    1936 
   Size:        987    4594
 
SbfI CC_TGCA'GG

Cuts at:   2933    2933 
   Size:       5581
 
ScaI AGT'ACT

Cuts at:   3829    5541    3829 
   Size:       1712    3869
 
SfcI C'TryA_G

Cuts at:    826    1045    2792    2846    2929    4064    4745    4936     826 
   Size:        219    1747      54      83    1135     681     191    1471

  Fragments arranged by size: 

                1747    1471    1135     681     219     191      83      54
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5581
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5581
 
SmaI CCC'GGG

Cuts at:   1872    2908    1872 
   Size:       1036    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2168    2448    3685    4586    4848     820 
   Size:        197     926     225     280    1237     901     262    1553

  Fragments arranged by size: 

                1553    1237     926     901     280     262     225     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3372     493 
   Size:       2879    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5581
 
SphI G_CATG'C

Cuts at:   1132    2939    1132 
   Size:       1807    3774
 
SspI AAT'ATT

Cuts at:      5      52    3505       5 
   Size:         47    3453    2081

  Fragments arranged by size: 

                3453    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5581
 
StyI C'CwwG_G

Cuts at:    513    1065    3020     513 
   Size:        552    1955    3074

  Fragments arranged by size: 

                3074    1955     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3608    3625    3778    3963    4408     325 
   Size:       1255    2028      17     153     185     445    1498

  Fragments arranged by size: 

                2028    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2538    3827    5539 
   Size:        274      80      33      51    1443     561    1289    1712

Cuts at:   5539      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1289     561     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2502    2534    5541    5573    2502 
   Size:         32    3007      32    2510

  Fragments arranged by size: 

                3007    2510      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5581
 
XbaI T'CTAG_A

Cuts at:   2917    2917 
   Size:       5581
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2743    2743 
   Size:       5581
 
XhoI C'TCGA_G

Cuts at:   1943    2448    1943 
   Size:        505    5076
 
XmaI C'CCGG_G

Cuts at:   1870    2906    1870 
   Size:       1036    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3710    3710 
   Size:       5581
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2209    3389     276 
   Size:         53      83     186     936     675    1180    2468

  Fragments arranged by size: 

                2468    1180     936     675     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PstI     PvuI    PvuII
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BbeI    BfrBI
     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I  EcoICRI    EcoRV
     FseI    FspAI     KasI     NarI     NruI     NsiI     PacI     PmlI
   PspOMI     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com