(Circular) (Six-base) MAPSORT of: pFN21AB8207.seq Check: 362 from: 1 to: 5581 pFN21AB8207 5581 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2211 3391 278 Size: 53 83 186 936 675 1180 2468 Fragments arranged by size: 2468 1180 936 675 186 83 53 AccI GT'mk_AC Cuts at: 1937 2924 1937 Size: 987 4594 Acc65I G'GTAC_C Cuts at: 2502 2902 2502 Size: 400 5181 AclI AA'CG_TT Cuts at: 1563 3708 4081 1563 Size: 2145 373 3063 Fragments arranged by size: 3063 2145 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2198 2285 2405 2825 3644 5028 5296 1632 Size: 566 87 120 420 819 1384 268 1917 Fragments arranged by size: 1917 1384 819 566 420 268 120 87 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5384 AflIII A'CryG_T Cuts at: 1203 2556 4480 5457 1203 Size: 1353 1924 977 1327 Fragments arranged by size: 1924 1353 1327 977 AgeI A'CCGG_T Cuts at: 4412 4412 Size: 5581 AhdI GACnn_n'nnGTC Cuts at: 2560 4310 2560 Size: 1750 3831 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5581 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5581 AlwNI CAG_nnn'CTG Cuts at: 2263 2398 3324 4896 2263 Size: 135 926 1572 2948 Fragments arranged by size: 2948 1572 926 135 ApaLI G'TGCA_C Cuts at: 3638 4794 3638 Size: 1156 4425 ApoI r'AATT_y Cuts at: 1493 2246 2890 3116 3268 5377 1493 Size: 753 644 226 152 2109 1697 Fragments arranged by size: 2109 1697 753 644 226 152 AseI AT'TA_AT Cuts at: 160 1032 4135 160 Size: 872 3103 1606 Fragments arranged by size: 3103 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5581 AvaI C'yCGr_G Cuts at: 1870 1943 2448 2906 1870 Size: 73 505 458 4545 Fragments arranged by size: 4545 505 458 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2486 2519 2486 Size: 33 5548 BamHI G'GATC_C Cuts at: 2911 3278 2911 Size: 367 5214 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2502 2902 4357 618 Size: 325 217 627 255 460 400 1455 1842 Fragments arranged by size: 1842 1455 627 460 400 325 255 217 BanII G_rGCy'C Cuts at: 1391 2900 1391 Size: 1509 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4610 BbvCI CC'TCA_GC Cuts at: 2484 2484 Size: 5581 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2608 2642 3772 3806 1903 Size: 34 50 34 587 34 1130 34 3678 Fragments arranged by size: 3678 1130 587 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3473 4683 3473 Size: 1210 4371 BclI T'GATC_A Cuts at: 1548 1548 Size: 5581 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4192 136 Size: 107 122 71 631 605 2520 1525 Fragments arranged by size: 2520 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2157 5576 2157 Size: 3419 2162 BlpI GC'TnA_GC Cuts at: 2998 2998 Size: 5581 Bme1580I G_kGCm'C Cuts at: 1790 2763 3642 4798 1790 Size: 973 879 1156 2573 Fragments arranged by size: 2573 1156 973 879 BmgBI CAC'GTC Cuts at: 1318 2547 1318 Size: 1229 4352 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3312 4270 442 Size: 416 53 83 399 24 1895 958 1753 Fragments arranged by size: 1895 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5581 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4241 1239 Size: 3002 2579 Bpu10I CC'TnA_GC Cuts at: 2484 2484 Size: 5581 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2153 3706 4571 4869 2153 Size: 1553 865 298 2865 Fragments arranged by size: 2865 1553 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4244 882 Size: 617 2745 2219 Fragments arranged by size: 2745 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3372 493 Size: 711 2168 2702 Fragments arranged by size: 2702 2168 711 BsaBI GATnn'nnATC Cuts at: 5575 5575 Size: 5581 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2208 2666 3388 3770 Size: 53 83 186 936 675 458 722 382 Cuts at: 3770 4405 275 Size: 635 1451 Fragments arranged by size: 1451 936 722 675 635 458 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2512 4014 4412 4686 4833 5244 2512 Size: 1502 398 274 147 411 2849 Fragments arranged by size: 2849 1502 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5356 Fragments arranged by size: 5356 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2690 1186 Size: 1504 4077 BseYI C'CCAG_C Cuts at: 2344 2597 2659 4784 5280 2344 Size: 253 62 2125 496 2645 Fragments arranged by size: 2645 2125 496 253 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 1163 Size: 573 462 4546 Fragments arranged by size: 4546 573 462 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3031 3792 4820 1540 Size: 275 186 1030 761 1028 2301 Fragments arranged by size: 2301 1030 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3642 3727 4798 3642 Size: 85 1071 4425 Fragments arranged by size: 4425 1071 85 BsmI GAATG_Cn' Cuts at: 3092 3185 3092 Size: 93 5488 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2763 2900 3642 3727 4798 1391 Size: 399 973 137 742 85 1071 2174 Fragments arranged by size: 2174 1071 973 742 399 137 85 BspEI T'CCGG_A Cuts at: 5244 5244 Size: 5581 BspHI T'CATG_A Cuts at: 2083 2203 3470 5200 2083 Size: 120 1267 1730 2464 Fragments arranged by size: 2464 1730 1267 120 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2406 2936 844 Size: 1562 530 3489 Fragments arranged by size: 3489 1562 530 BsrBI CCG'CTC Cuts at: 1394 1823 3468 1394 Size: 429 1645 3507 Fragments arranged by size: 3507 1645 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2293 2371 2626 4076 4250 65 Size: 1183 1045 78 255 1450 174 1396 Fragments arranged by size: 1450 1396 1183 1045 255 174 78 BsrFI r'CCGG_y Cuts at: 1700 1810 2567 4225 4412 1700 Size: 110 757 1658 187 2869 Fragments arranged by size: 2869 1658 757 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5581 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5581 BssSI C'ACGA_G Cuts at: 3641 4653 3641 Size: 1012 4569 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5581 BstBI TT'CG_AA Cuts at: 4385 4385 Size: 5581 BstEII G'GTnAC_C Cuts at: 1184 2291 1184 Size: 1107 4474 BstYI r'GATC_y Cuts at: 1972 2157 2646 2785 2911 3278 3663 3680 5132 Size: 185 489 139 126 367 385 17 1452 Cuts at: 5132 5218 5230 5576 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 489 385 367 346 185 139 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5029 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4842 Fragments arranged by size: 4842 683 56 BtsI GCAGTG_nn' Cuts at: 2687 3097 3891 3911 2687 Size: 410 794 20 4357 Fragments arranged by size: 4357 794 410 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5581 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5546 DraI TTT'AAA Cuts at: 2885 2885 Size: 5581 DraIII CAC_nnn'GTG Cuts at: 2499 2499 Size: 5581 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4588 809 Size: 507 3272 1802 Fragments arranged by size: 3272 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2589 2733 3028 Size: 54 1425 50 275 18 759 144 295 Cuts at: 3028 3917 5546 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 759 295 275 144 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3028 1537 Size: 275 1216 4090 Fragments arranged by size: 4090 1216 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2330 2636 2832 3511 1353 Size: 977 306 196 679 3423 Fragments arranged by size: 3423 977 679 306 196 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2134 2865 4168 4542 4688 387 Size: 1064 138 545 731 1303 374 146 1280 Fragments arranged by size: 1303 1280 1064 731 545 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2285 2405 2825 3644 4241 5028 Size: 393 566 87 120 420 819 597 787 Cuts at: 5028 5296 1239 Size: 268 1524 Fragments arranged by size: 1524 819 787 597 566 420 393 268 120 87 EcoNI CCTnn'n_nnAGG Cuts at: 2168 2168 Size: 5581 EcoO109I rG'GnC_Cy Cuts at: 2260 2380 2842 2260 Size: 120 462 4999 Fragments arranged by size: 4999 462 120 EcoRI G'AATT_C Cuts at: 2246 2890 3268 2246 Size: 644 378 4559 Fragments arranged by size: 4559 644 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2665 2665 Size: 5581 FspI TGC'GCA Cuts at: 4087 4087 Size: 5581 HaeII r_GCGC'y Cuts at: 1678 4728 1678 Size: 3050 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2080 2112 2546 2578 4222 Size: 32 135 32 508 32 434 32 1644 Cuts at: 4222 4254 4296 4328 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1644 508 434 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2925 3171 669 Size: 988 281 987 246 3079 Fragments arranged by size: 3079 988 987 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5581 HpaI GTT'AAC Cuts at: 3171 3171 Size: 5581 KpnI G_GTAC'C Cuts at: 2506 2906 2506 Size: 400 5181 MfeI C'AATT_G Cuts at: 3180 3180 Size: 5581 MluI A'CGCG_T Cuts at: 5457 5457 Size: 5581 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2677 2889 4695 4879 1305 Size: 1372 212 1806 184 2007 Fragments arranged by size: 2007 1806 1372 212 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2591 10 Size: 54 1425 343 759 3000 Fragments arranged by size: 3000 1425 759 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2555 3539 3898 4057 518 Size: 648 1389 984 359 159 2042 Fragments arranged by size: 2042 1389 984 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2472 2483 2997 3674 4822 5067 1606 Size: 866 11 514 677 1148 245 2120 Fragments arranged by size: 2120 1148 866 677 514 245 11 NaeI GCC'GGC Cuts at: 2569 2569 Size: 5581 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5029 NdeI CA'TA_TG Cuts at: 387 387 Size: 5581 NgoMIV G'CCGG_C Cuts at: 2567 2567 Size: 5581 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5581 NotI GC'GGCC_GC Cuts at: 3028 3028 Size: 5581 NspI r_CATG'y Cuts at: 1132 1229 2560 2939 4484 1132 Size: 97 1331 379 1545 2229 Fragments arranged by size: 2229 1545 1331 379 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5581 PciI A'CATG_T Cuts at: 2556 4480 2556 Size: 1924 3657 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5581 PfoI T'CCnGG_A Cuts at: 2151 2151 Size: 5581 PmeI GTTT'AAAC Cuts at: 2885 2885 Size: 5581 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3650 3682 5163 5195 3650 Size: 32 1481 32 4036 Fragments arranged by size: 4036 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2260 2380 2260 Size: 120 5461 PshAI GACnn'nnGTC Cuts at: 1613 2871 1613 Size: 1258 4323 PsiI TTA'TAA Cuts at: 3151 3151 Size: 5581 PspXI vC'TCGA_Gb Cuts at: 2448 2448 Size: 5581 PstI C_TGCA'G Cuts at: 830 2796 2850 2933 830 Size: 1966 54 83 3478 Fragments arranged by size: 3478 1966 83 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5581 PvuII CAG'CTG Cuts at: 2483 2483 Size: 5581 SalI G'TCGA_C Cuts at: 1936 2923 1936 Size: 987 4594 SbfI CC_TGCA'GG Cuts at: 2933 2933 Size: 5581 ScaI AGT'ACT Cuts at: 3829 5541 3829 Size: 1712 3869 SfcI C'TryA_G Cuts at: 826 1045 2792 2846 2929 4064 4745 4936 826 Size: 219 1747 54 83 1135 681 191 1471 Fragments arranged by size: 1747 1471 1135 681 219 191 83 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5581 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5581 SmaI CCC'GGG Cuts at: 1872 2908 1872 Size: 1036 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2168 2448 3685 4586 4848 820 Size: 197 926 225 280 1237 901 262 1553 Fragments arranged by size: 1553 1237 926 901 280 262 225 197 SnaBI TAC'GTA Cuts at: 493 3372 493 Size: 2879 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5581 SphI G_CATG'C Cuts at: 1132 2939 1132 Size: 1807 3774 SspI AAT'ATT Cuts at: 5 52 3505 5 Size: 47 3453 2081 Fragments arranged by size: 3453 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5581 StyI C'CwwG_G Cuts at: 513 1065 3020 513 Size: 552 1955 3074 Fragments arranged by size: 3074 1955 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3608 3625 3778 3963 4408 325 Size: 1255 2028 17 153 185 445 1498 Fragments arranged by size: 2028 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2538 3827 5539 Size: 274 80 33 51 1443 561 1289 1712 Cuts at: 5539 96 Size: 138 Fragments arranged by size: 1712 1443 1289 561 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2502 2534 5541 5573 2502 Size: 32 3007 32 2510 Fragments arranged by size: 3007 2510 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5581 XbaI T'CTAG_A Cuts at: 2917 2917 Size: 5581 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2743 2743 Size: 5581 XhoI C'TCGA_G Cuts at: 1943 2448 1943 Size: 505 5076 XmaI C'CCGG_G Cuts at: 1870 2906 1870 Size: 1036 4545 XmnI GAAnn'nnTTC Cuts at: 3710 3710 Size: 5581 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2209 3389 276 Size: 53 83 186 936 675 1180 2468 Fragments arranged by size: 2468 1180 936 675 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI