(Circular) (Six-base) MAPSORT of: pFN21AB8202.seq Check: 8055 from: 1 to: 5287 pFN21AB8202 5287 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3097 278 Size: 53 83 186 936 1561 2468 Fragments arranged by size: 2468 1561 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2630 1937 Size: 693 4594 Acc65I G'GTAC_C Cuts at: 2608 2608 Size: 5287 AclI AA'CG_TT Cuts at: 1563 3414 3787 1563 Size: 1851 373 3063 Fragments arranged by size: 3063 1851 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3350 4734 5002 1632 Size: 1718 1384 268 1917 Fragments arranged by size: 1917 1718 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5090 AflIII A'CryG_T Cuts at: 1203 4186 5163 1203 Size: 2983 977 1327 Fragments arranged by size: 2983 1327 977 AgeI A'CCGG_T Cuts at: 4118 4118 Size: 5287 AhdI GACnn_n'nnGTC Cuts at: 4016 4016 Size: 5287 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5287 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5287 AlwNI CAG_nnn'CTG Cuts at: 2183 3030 4602 2183 Size: 847 1572 2868 Fragments arranged by size: 2868 1572 847 ApaLI G'TGCA_C Cuts at: 3344 4500 3344 Size: 1156 4131 ApoI r'AATT_y Cuts at: 1493 2011 2288 2596 2822 2974 5083 1493 Size: 518 277 308 226 152 2109 1697 Fragments arranged by size: 2109 1697 518 308 277 226 152 AseI AT'TA_AT Cuts at: 160 1032 3841 160 Size: 872 2809 1606 Fragments arranged by size: 2809 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5287 AvaI C'yCGr_G Cuts at: 1870 1943 2612 1870 Size: 73 669 4545 Fragments arranged by size: 4545 669 73 BamHI G'GATC_C Cuts at: 2560 2617 2984 2560 Size: 57 367 4863 Fragments arranged by size: 4863 367 57 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2608 4063 618 Size: 325 217 627 821 1455 1842 Fragments arranged by size: 1842 1455 821 627 325 217 BanII G_rGCy'C Cuts at: 1391 2035 2606 1391 Size: 644 571 4072 Fragments arranged by size: 4072 644 571 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4316 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3478 3512 1903 Size: 34 1541 34 3678 Fragments arranged by size: 3678 1541 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3179 4389 3179 Size: 1210 4077 BclI T'GATC_A Cuts at: 1548 2253 1548 Size: 705 4582 BfrBI ATG'CAT Cuts at: 2503 2503 Size: 5287 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3898 136 Size: 107 122 71 631 605 2226 1525 Fragments arranged by size: 2226 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5282 5282 Size: 5287 BlpI GC'TnA_GC Cuts at: 2704 2704 Size: 5287 Bme1580I G_kGCm'C Cuts at: 1790 3348 4504 1790 Size: 1558 1156 2573 Fragments arranged by size: 2573 1558 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5287 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2577 3018 3976 Size: 416 53 83 399 24 1160 441 958 Cuts at: 3976 442 Size: 1753 Fragments arranged by size: 1753 1160 958 441 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5287 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3947 1239 Size: 2708 2579 Bpu10I CC'TnA_GC Cuts at: 2034 2034 Size: 5287 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3412 4277 4575 3412 Size: 865 298 4124 Fragments arranged by size: 4124 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3950 882 Size: 617 2451 2219 Fragments arranged by size: 2451 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3078 493 Size: 711 1874 2702 Fragments arranged by size: 2702 1874 711 BsaBI GATnn'nnATC Cuts at: 5281 5281 Size: 5287 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3094 3476 4111 275 Size: 53 83 186 936 1561 382 635 1451 Fragments arranged by size: 1561 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3720 4118 4392 4539 4950 3720 Size: 398 274 147 411 4057 Fragments arranged by size: 4057 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2143 2173 1178 Size: 30 165 30 740 30 4292 Fragments arranged by size: 4292 740 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2148 1186 Size: 962 4325 BseYI C'CCAG_C Cuts at: 4490 4986 4490 Size: 496 4791 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4714 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2737 3498 4526 1540 Size: 275 186 736 761 1028 2301 Fragments arranged by size: 2301 1028 761 736 275 186 BsiHKAI G_wGCw'C Cuts at: 3348 3433 4504 3348 Size: 85 1071 4131 Fragments arranged by size: 4131 1071 85 BsmI GAATG_Cn' Cuts at: 2798 2891 2798 Size: 93 5194 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2606 3348 3433 4504 1391 Size: 399 245 571 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 571 399 245 85 BspEI T'CCGG_A Cuts at: 4950 4950 Size: 5287 BspHI T'CATG_A Cuts at: 2205 3176 4906 2205 Size: 971 1730 2586 Fragments arranged by size: 2586 1730 971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2346 2642 844 Size: 1502 296 3489 Fragments arranged by size: 3489 1502 296 BsrBI CCG'CTC Cuts at: 1394 1823 2388 3174 1394 Size: 429 565 786 3507 Fragments arranged by size: 3507 786 565 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3782 3956 65 Size: 1183 2534 174 1396 Fragments arranged by size: 2534 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2453 3931 4118 1700 Size: 110 643 1478 187 2869 Fragments arranged by size: 2869 1478 643 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5287 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5287 BssSI C'ACGA_G Cuts at: 3347 4359 3347 Size: 1012 4275 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5287 BstBI TT'CG_AA Cuts at: 4091 4091 Size: 5287 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5287 BstYI r'GATC_y Cuts at: 1972 2560 2617 2984 3369 3386 4838 4924 4936 Size: 588 57 367 385 17 1452 86 12 Cuts at: 4936 5282 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 588 385 367 346 86 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2145 2151 513 Size: 552 939 141 6 3649 Fragments arranged by size: 3649 939 552 141 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4548 Fragments arranged by size: 4548 683 56 BtsI GCAGTG_nn' Cuts at: 2495 2803 3597 3617 2495 Size: 308 794 20 4165 Fragments arranged by size: 4165 794 308 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5287 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5252 DraI TTT'AAA Cuts at: 2591 2591 Size: 5287 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4294 809 Size: 507 2978 1802 Fragments arranged by size: 2978 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2451 2734 Size: 54 1425 50 275 18 318 303 283 Cuts at: 2734 3623 5252 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 318 303 283 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2734 1537 Size: 275 922 4090 Fragments arranged by size: 4090 922 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3217 1353 Size: 1864 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2450 3874 4248 4394 387 Size: 1064 138 861 1424 374 146 1280 Fragments arranged by size: 1424 1280 1064 861 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3350 3947 4734 5002 1239 Size: 393 1718 597 787 268 1524 Fragments arranged by size: 1718 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2436 2436 Size: 5287 EcoO109I rG'GnC_Cy Cuts at: 2120 2120 Size: 5287 EcoRI G'AATT_C Cuts at: 2596 2974 2596 Size: 378 4909 FspI TGC'GCA Cuts at: 3793 3793 Size: 5287 HaeII r_GCGC'y Cuts at: 1678 4434 1678 Size: 2756 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2338 2370 3928 3960 4002 Size: 32 135 32 766 32 1558 32 42 Cuts at: 4002 4034 1373 Size: 32 2626 Fragments arranged by size: 2626 1558 766 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2631 2877 669 Size: 988 281 693 246 3079 Fragments arranged by size: 3079 988 693 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5287 HpaI GTT'AAC Cuts at: 2877 2877 Size: 5287 KpnI G_GTAC'C Cuts at: 2612 2612 Size: 5287 MfeI C'AATT_G Cuts at: 2886 2886 Size: 5287 MluI A'CGCG_T Cuts at: 5163 5163 Size: 5287 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4401 4585 1305 Size: 3096 184 2007 Fragments arranged by size: 3096 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3465 Fragments arranged by size: 3465 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3245 3604 3763 518 Size: 648 2079 359 159 2042 Fragments arranged by size: 2079 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2147 2703 3380 4528 4773 1606 Size: 541 556 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 556 541 245 NaeI GCC'GGC Cuts at: 2455 2455 Size: 5287 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3796 Fragments arranged by size: 3796 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5287 NgoMIV G'CCGG_C Cuts at: 2453 2453 Size: 5287 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5287 NotI GC'GGCC_GC Cuts at: 2734 2734 Size: 5287 NsiI A_TGCA'T Cuts at: 2505 2505 Size: 5287 NspI r_CATG'y Cuts at: 1132 1229 2645 4190 1132 Size: 97 1416 1545 2229 Fragments arranged by size: 2229 1545 1416 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5287 PciI A'CATG_T Cuts at: 4186 4186 Size: 5287 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5287 PmeI GTTT'AAAC Cuts at: 2591 2591 Size: 5287 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3356 3388 4869 4901 3356 Size: 32 1481 32 3742 Fragments arranged by size: 3742 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2120 2120 Size: 5287 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5287 PsiI TTA'TAA Cuts at: 2857 2857 Size: 5287 PstI C_TGCA'G Cuts at: 830 2639 830 Size: 1809 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5287 SacII CC_GC'GG Cuts at: 2148 2148 Size: 5287 SalI G'TCGA_C Cuts at: 1936 2629 1936 Size: 693 4594 SanDI GG'GwC_CC Cuts at: 2120 2120 Size: 5287 SbfI CC_TGCA'GG Cuts at: 2639 2639 Size: 5287 ScaI AGT'ACT Cuts at: 3535 5247 3535 Size: 1712 3575 SfcI C'TryA_G Cuts at: 826 1045 2635 3770 4451 4642 826 Size: 219 1590 1135 681 191 1471 Fragments arranged by size: 1590 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5287 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5287 SmaI CCC'GGG Cuts at: 1872 2614 1872 Size: 742 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3391 4292 4554 820 Size: 197 926 1448 901 262 1553 Fragments arranged by size: 1553 1448 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3078 493 Size: 2585 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5287 SphI G_CATG'C Cuts at: 1132 2645 1132 Size: 1513 3774 SspI AAT'ATT Cuts at: 5 52 3211 5 Size: 47 3159 2081 Fragments arranged by size: 3159 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5287 StyI C'CwwG_G Cuts at: 513 1065 2004 2726 513 Size: 552 939 722 3074 Fragments arranged by size: 3074 939 722 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3314 3331 3484 3669 4114 325 Size: 1255 1734 17 153 185 445 1498 Fragments arranged by size: 1734 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3533 5245 96 Size: 274 80 33 51 1443 1556 1712 138 Fragments arranged by size: 1712 1556 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2143 2175 5247 5279 2143 Size: 32 3072 32 2151 Fragments arranged by size: 3072 2151 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5287 XbaI T'CTAG_A Cuts at: 2623 2623 Size: 5287 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5287 XmaI C'CCGG_G Cuts at: 1870 2612 1870 Size: 742 4545 XmnI GAAnn'nnTTC Cuts at: 3416 3416 Size: 5287 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3095 276 Size: 53 83 186 936 1561 2468 Fragments arranged by size: 2468 1561 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI