Restriction map for
pFN21AB8201
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BciVI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstPI BstUI
BstXI Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MseI MslI MspA1I MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI
SgrAI SnaBI SphI Sse8387I SspBI
StuI SwaI Tru9I Tth111I Van91I
XbaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 259 GTATAC GT^[AC][GT]AC
AfaI 557 GTAC GT^AC
AlwI 138 GATCC GGATC(4/5)
Asp700I 279 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 597 GTGCTC G[AT]GC[AT]^C
BbvI 33 GCTGC GCAGC(8/12)
BcgI 444 CGAAACTAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 71 TGATCA T^GATCA
BmyI 597 GTGCTC G[AGT]GC[ACT]^C
BsgI 391 GTGCAG GTGCAG(16/14)
BsiHKAI 597 GTGCTC G[AT]GC[AT]^C
BsmI 324 GAATGC GAATGC(1/-1)
Bsp1286I 597 GTGCTC G[AGT]GC[ACT]^C
BsrBI 202 GAGCGG CCGCTC(-3/-3)
Bst1107I 259 GTATAC GTA^TAC
BstBI 442 TTCGAA TT^CGAA
BstYI 137 AGATCC [GA]^GATC[CT]
BstZ17I 259 GTATAC GTA^TAC
CfoI 345 GCGC GCG^C
Cfr13I 495 GGCCC G^G[ACGT]CC
EarI 22 CTCTTC CTCTTC(1/4)
EcoT14I 242 CCATGG C^C[AT][AT]GG
FbaI 71 TGATCA T^GATCA
Fnu4HI 33 GCTGC GC^[ACGT]GC
HaeII 344 AGCGCC [GA]GCGC^[CT]
HhaI 345 GCGC GCG^C
HinP1I 345 GCGC G^CGC
ItaI 33 GCTGC GC^[ACGT]GC
Ksp632I 22 CTCTTC CTCTTC(1/4)
MflI 137 AGATCC [GA]^GATC[CT]
NcoI 242 CCATGG C^CATGG
NlaIV 238 GGAGCC GG[ACGT]^[ACGT]CC
NspV 442 TTCGAA TT^CGAA
PaeR7I 419 CTCGAG C^TCGAG
PleI 46 GACTC GAGTC(4/5)
RsaI 557 GTAC GT^AC
Sau96I 495 GGCCC G^G[ACGT]CC
ScaI 556 AGTACT AGT^ACT
SfaNI 314 GCATC GCATC(5/9)
SfuI 442 TTCGAA TT^CGAA
SmaI 497 CCCGGG CCC^GGG
SpeI 448 ACTAGT A^CTAGT
SspI 292 AATATT AAT^ATT
StyI 242 CCATGG C^C[AT][AT]GG
TfiI 431 GAATC G^A[AT]TC
TseI 33 GCTGC G^C[AT]GC
Tsp509I 126 AATT ^AATT
TspRI 144 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 461 CCAGTATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 419 CTCGAG C^TCGAG
XhoII 137 AGATCC [GA]^GATC[CT]
XmaCI 497 CCCGGG C^CCGGG
XmaI 497 CCCGGG C^CCGGG
XmnI 279 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 141 CCGC CCGC(-3/-1)
204 GCGG
BalI 2 TGGCCA TGG^CCA
195 TGGCCA
BcnI 497 CCCGG CC^[GC]GG
498 CCGGG
BfaI 256 CTAG C^TAG
449 CTAG
BstNI 492 CCTGG CC^[AT]GG
603 CCTGG
Cac8I 193 GCTGGC GC[ACGT]^[ACGT]GC
402 GCAGGC
DsaI 234 CCGTGG C^C[GA][CT]GG
242 CCATGG
EaeI 2 TGGCCA [CT]^GGCC[GA]
195 TGGCCA
EcoRII 492 CCTGG ^CC[AT]GG
603 CCTGG
HapII 498 CCGG C^CGG
525 CCGG
HinfI 46 GACTC G^A[ACGT]TC
431 GAATC
HpaII 498 CCGG C^CGG
525 CCGG
MaeI 256 CTAG C^TAG
449 CTAG
MaeIII 368 GTGAC ^GT[ACGT]AC
575 GTGAC
MluNI 2 TGGCCA TGG^CCA
195 TGGCCA
MscI 2 TGGCCA TGG^CCA
195 TGGCCA
MspI 498 CCGG C^CGG
525 CCGG
MvaI 492 CCTGG CC^[AT]GG
603 CCTGG
NciI 497 CCCGG CC^[GC]GG
498 CCGGG
SmlI 419 CTCGAG C^T[CT][GA]AG
511 CTCAAG
Tsp45I 368 GTGAC ^GT[GC]AC
575 GTGAC
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AvaI BsaJI BsiYI BslI BsmFI
BsoBI BsrI BssKI BstF5I DpnII
FokI HaeIII MboI MboII MnlI
MwoI NdeII NlaIII Sau3AI ScrFI
TaqI TthHB8I