Restriction map for
pFN21AB8200
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BcgI BciVI BclI BfrI BglI
BlnI BpmI BpuAI BsaI BsaWI
BseAI BseRI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI Eam1105I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI MfeI
MluI MluNI MroI MscI MseI
MunI NarI NcoI NdeI NheI
NotI NsiI NspI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AfaI 613 GTAC GT^AC
AflIII 230 ACGTGT A^C[GA][CT]GT
Alw44I 133 GTGCAC G^TGCAC
Aor51HI 899 AGCGCT AGC^GCT
ApaLI 133 GTGCAC G^TGCAC
AvaI 184 CCCGAG C^[CT]CG[GA]G
BbvCI 435 CCTCAGC CCTCAGC(-5/-2)
BfaI 961 CTAG C^TAG
BglII 992 AGATCT A^GATCT
BsaAI 229 TACGTG [CT]AC^GT[GA]
BsiEI 535 CGGCCG CG[GA][CT]^CG
BsoBI 184 CCCGAG C^[CT]CG[GA]G
BstBI 586 TTCGAA TT^CGAA
BstXI 1040 CCACTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 546 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 667 GACAGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 535 CGGCCG C^GGCCG
EarI 1077 GAAGAG CTCTTC(1/4)
EclXI 535 CGGCCG C^GGCCG
Eco47III 899 AGCGCT AGC^GCT
Eco52I 535 CGGCCG C^GGCCG
EcoT14I 128 CCAAGG C^C[AT][AT]GG
Ksp632I 1077 GAAGAG CTCTTC(1/4)
KspI 824 CCGCGG CCGC^GG
MaeI 961 CTAG C^TAG
NruI 710 TCGCGA TCG^CGA
NspV 586 TTCGAA TT^CGAA
PpuMI 1100 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 33 CAGCTG CAG^CTG
RsaI 613 GTAC GT^AC
SacII 824 CCGCGG CCGC^GG
SapI 1077 GAAGAGC GCTCTTC(1/4)
SfuI 586 TTCGAA TT^CGAA
StyI 128 CCAAGG C^C[AT][AT]GG
TfiI 589 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 619 GGCGTC G[GA]^CG[CT]C
817 GACGCC
AlwI 281 GGATC GGATC(4/5)
500 GGATC
AlwNI 597 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
907 CAGAACCTG
ApaI 179 GGGCCC GGGCC^C
311 GGGCCC
BcnI 288 CCGGG CC^[GC]GG
798 CCCGG
BlpI 834 GCTGAGC GC^T[ACGT]AGC
1002 GCTCAGC
Bpu1102I 834 GCTGAGC GC^T[ACGT]AGC
1002 GCTCAGC
BsaBI 66 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
357 GATCTCCATC
BsaHI 619 GGCGTC G[GA]^CG[CT]C
817 GACGCC
BsgI 970 CTGCAC GTGCAG(16/14)
1035 CTGCAC
BspMI 205 ACCTGC ACCTGC(4/8)
159 GCAGGT
BsrBI 423 CCGCTC CCGCTC(-3/-3)
111 GAGCGG
BsrFI 475 GCCGGC [GA]^CCGG[CT]
537 GCCGGC
BssSI 136 CACGAG CACGAG(-5/-1)
350 CTCGTG
BstAPI 596 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
671 GCACCGTCTGC
BstYI 500 GGATCT [GA]^GATC[CT]
992 AGATCT
CelII 834 GCTGAGC GC^T[ACGT]AGC
1002 GCTCAGC
Cfr10I 475 GCCGGC [GA]^CCGG[CT]
537 GCCGGC
DsaI 550 CCGTGG C^C[GA][CT]GG
824 CCGCGG
EaeI 535 CGGCCG [CT]^GGCC[GA]
704 TGGCCG
HaeII 744 GGCGCT [GA]GCGC^[CT]
899 AGCGCT
Hin1I 619 GGCGTC G[GA]^CG[CT]C
817 GACGCC
HphI 162 GGTGA GGTGA(8/7)
809 TCACC
MaeII 230 ACGT A^CGT
857 ACGT
MamI 66 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
357 GATCTCCATC
MboII 699 GAAGA GAAGA(8/7)
1077 GAAGA
MflI 500 GGATCT [GA]^GATC[CT]
992 AGATCT
MslI 97 CATATCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1041 CACTGGCATG
NaeI 475 GCCGGC GCC^GGC
537 GCCGGC
NciI 288 CCGGG CC^[GC]GG
798 CCCGG
NgoMIV 475 GCCGGC G^CCGGC
537 GCCGGC
PflMI 545 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
906 CCAGAACCTGG
SmlI 142 CTCAAG C^T[CT][GA]AG
1105 CTCAAG
TspRI 737 CACTG CA[GC]TG[ACGT][ACGT]^
1041 CACTG
Van91I 545 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
906 CCAGAACCTGG
XhoII 500 GGATCT [GA]^GATC[CT]
992 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanI BanII BbvI BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI MaeIII MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
SacI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI Tsp45I TthHB8I