Restriction map for
pFN21AB8200
(Linear) (Six-base) MAPSORT of: KIBB8200.seq Check: 3761 from: 1 to: 1116
KIBB8200.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 150 1116
Size: 150 966
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1116
Size: 262 854
AfeI AGC'GCT
Cuts at: 0 901 1116
Size: 901 215
AflIII A'CryG_T
Cuts at: 0 230 1116
Size: 230 886
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 568 600 1116
Size: 568 32 516
Fragments arranged by size:
568 516 32
AlwNI CAG_nnn'CTG
Cuts at: 0 602 912 1116
Size: 602 310 204
Fragments arranged by size:
602 310 204
ApaI G_GGCC'C
Cuts at: 0 183 315 1116
Size: 183 132 801
Fragments arranged by size:
801 183 132
ApaLI G'TGCA_C
Cuts at: 0 133 1116
Size: 133 983
AvaI C'yCGr_G
Cuts at: 0 184 1116
Size: 184 932
BanI G'GyrC_C
Cuts at: 0 115 449 540 1116
Size: 115 334 91 576
Fragments arranged by size:
576 334 115 91
BanII G_rGCy'C
Cuts at: 0 29 143 183 315 559 874 931 1116
Size: 29 114 40 132 244 315 57 185
Fragments arranged by size:
315 244 185 132 114 57 40 29
BbvCI CC'TCA_GC
Cuts at: 0 436 1116
Size: 436 680
BglII A'GATC_T
Cuts at: 0 992 1116
Size: 992 124
BlpI GC'TnA_GC
Cuts at: 0 835 1003 1116
Size: 835 168 113
Fragments arranged by size:
835 168 113
Bme1580I G_kGCm'C
Cuts at: 0 137 183 315 1116
Size: 137 46 132 801
Fragments arranged by size:
801 137 132 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 186 1116
Size: 186 930
Bpu10I CC'TnA_GC
Cuts at: 0 436 863 1116
Size: 436 427 253
Fragments arranged by size:
436 427 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 1090 1116
Size: 127 963 26
Fragments arranged by size:
963 127 26
BsaAI yAC'GTr
Cuts at: 0 231 1116
Size: 231 885
BsaBI GATnn'nnATC
Cuts at: 0 70 361 1116
Size: 70 291 755
Fragments arranged by size:
755 291 70
BsaHI Gr'CG_yC
Cuts at: 0 620 818 1116
Size: 620 198 298
Fragments arranged by size:
620 298 198
BseYI C'CCAG_C
Cuts at: 0 308 608 1116
Size: 308 300 508
Fragments arranged by size:
508 308 300
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1020 1116
Size: 955 65 96
Fragments arranged by size:
955 96 65
BsiEI CG_ry'CG
Cuts at: 0 538 1116
Size: 538 578
BsiHKAI G_wGCw'C
Cuts at: 0 137 143 152 874 931 1004 1027 1116
Size: 137 6 9 722 57 73 23 89
Fragments arranged by size:
722 137 89 73 57 23 9 6
Bsp1286I G_dGCh'C
Cuts at: 0 29 137 143 152 183 315 559 874
Size: 29 108 6 9 31 132 244 315
Cuts at: 874 931 1004 1027 1116
Size: 57 73 23 89
Fragments arranged by size:
315 244 132 108 89 73 57 31
29 23 9 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 150 214 1116
Size: 150 64 902
Fragments arranged by size:
902 150 64
BsrBI CCG'CTC
Cuts at: 0 113 425 1116
Size: 113 312 691
Fragments arranged by size:
691 312 113
BsrFI r'CCGG_y
Cuts at: 0 475 537 1116
Size: 475 62 579
Fragments arranged by size:
579 475 62
BssSI C'ACGA_G
Cuts at: 0 136 350 1116
Size: 136 214 766
Fragments arranged by size:
766 214 136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 602 677 1116
Size: 602 75 439
Fragments arranged by size:
602 439 75
BstBI TT'CG_AA
Cuts at: 0 587 1116
Size: 587 529
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1047 1116
Size: 1047 69
BstYI r'GATC_y
Cuts at: 0 500 992 1116
Size: 500 492 124
Fragments arranged by size:
500 492 124
BtgI C'CryG_G
Cuts at: 0 550 824 1116
Size: 550 274 292
Fragments arranged by size:
550 292 274
BtsI GCAGTG_nn'
Cuts at: 0 736 1116
Size: 736 380
DraIII CAC_nnn'GTG
Cuts at: 0 551 1116
Size: 551 565
DrdI GACnn_nn'nnGTC
Cuts at: 0 673 1116
Size: 673 443
EaeI y'GGCC_r
Cuts at: 0 535 704 1116
Size: 535 169 412
Fragments arranged by size:
535 412 169
EagI C'GGCC_G
Cuts at: 0 535 1116
Size: 535 581
EarI CTCTTCn'nnn_
Cuts at: 0 1072 1116
Size: 1072 44
EcoICRI GAG'CTC
Cuts at: 0 141 872 929 1116
Size: 141 731 57 187
Fragments arranged by size:
731 187 141 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1116
Size: 262 854
EcoO109I rG'GnC_Cy
Cuts at: 0 180 312 1101 1116
Size: 180 132 789 15
Fragments arranged by size:
789 180 132 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 585 1116
Size: 585 531
HaeII r_GCGC'y
Cuts at: 0 748 903 1116
Size: 748 155 213
Fragments arranged by size:
748 213 155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 1116
Size: 478 638
MslI CAynn'nnrTG
Cuts at: 0 101 1045 1116
Size: 101 944 71
Fragments arranged by size:
944 101 71
MspA1I CmG'CkG
Cuts at: 0 35 299 344 638 781 826 1007 1116
Size: 35 264 45 294 143 45 181 109
Fragments arranged by size:
294 264 181 143 109 45 45 35
NaeI GCC'GGC
Cuts at: 0 477 539 1116
Size: 477 62 577
Fragments arranged by size:
577 477 62
NgoMIV G'CCGG_C
Cuts at: 0 475 537 1116
Size: 475 62 579
Fragments arranged by size:
579 475 62
NruI TCG'CGA
Cuts at: 0 712 1116
Size: 712 404
PflMI CCAn_nnn'nTGG
Cuts at: 0 551 912 1116
Size: 551 361 204
Fragments arranged by size:
551 361 204
PpuMI rG'GwC_Cy
Cuts at: 0 1101 1116
Size: 1101 15
PspOMI G'GGCC_C
Cuts at: 0 179 311 1116
Size: 179 132 805
Fragments arranged by size:
805 179 132
PvuII CAG'CTG
Cuts at: 0 35 1116
Size: 35 1081
SacI G_AGCT'C
Cuts at: 0 143 874 931 1116
Size: 143 731 57 185
Fragments arranged by size:
731 185 143 57
SacII CC_GC'GG
Cuts at: 0 827 1116
Size: 827 289
SapI GCTCTTCn'nnn_
Cuts at: 0 1072 1116
Size: 1072 44
SmlI C'TyrA_G
Cuts at: 0 142 1105 1116
Size: 142 963 11
Fragments arranged by size:
963 142 11
StyI C'CwwG_G
Cuts at: 0 128 1116
Size: 128 988
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 76 558 1116
Size: 76 482 558
Fragments arranged by size:
558 482 76
Enzymes that do cut:
AarI AcuI AfeI AflIII AloI AlwNI ApaI ApaLI
AvaI BanI BanII BbvCI BglII BlpI Bme1580I BmrI
Bpu10I BpuEI BsaAI BsaBI BsaHI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtsI DraIII DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoO109I FalI HaeII MmeI MslI
MspA1I NaeI NgoMIV NruI PflMI PpuMI PspOMI PvuII
SacI SacII SapI SmlI StyI TaqII
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BcgI BciVI BclI BfrBI BglI BmgBI
BmtI BplI BpmI BsaI BsaWI BsaXI BseRI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NarI NcoI
NdeI NheI NotI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI