Restriction map for pFN21AB8191
 (Circular) (Six-base) MAPSORT of: pFN21AB8191.seq  Check: 4218  from: 1  to: 5980



pFN21AB8191  5980 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:41  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2221    2221 
   Size:       5980
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3790     278 
   Size:         53      83     186     936    2254    2468

  Fragments arranged by size: 

                2468    2254     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3093    3323    1937 
   Size:       1156     230    4594

  Fragments arranged by size: 

                4594    1156     230
 
Acc65I G'GTAC_C

Cuts at:   3301    3301 
   Size:       5980
 
AclI AA'CG_TT

Cuts at:   1563    4107    4480    1563 
   Size:       2544     373    3063

  Fragments arranged by size: 

                3063    2544     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3044    4043    5427    5695    1632 
   Size:       1412     999    1384     268    1917

  Fragments arranged by size: 

                1917    1412    1384     999     268
 
AfeI AGC'GCT

Cuts at:   2807    2938    2972    2807 
   Size:        131      34    5815

  Fragments arranged by size: 

                5815     131      34
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5783
 
AflIII A'CryG_T

Cuts at:   1203    4879    5856    1203 
   Size:       3676     977    1327

  Fragments arranged by size: 

                3676    1327     977
 
AgeI A'CCGG_T

Cuts at:   4811    4811 
   Size:       5980
 
AhdI GACnn_n'nnGTC

Cuts at:   4709    4709 
   Size:       5980
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5980
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5980
 
AlwNI CAG_nnn'CTG

Cuts at:   3723    5295    3723 
   Size:       1572    4408
 
ApaLI G'TGCA_C

Cuts at:   3112    4037    5193    3112 
   Size:        925    1156    3899

  Fragments arranged by size: 

                3899    1156     925
 
ApoI r'AATT_y

Cuts at:   1493    3289    3515    3667    5776    1493 
   Size:       1796     226     152    2109    1697

  Fragments arranged by size: 

                2109    1796    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4534     160 
   Size:        872    3502    1606

  Fragments arranged by size: 

                3502    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5980
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3305    1870 
   Size:         73    1362    4545

  Fragments arranged by size: 

                4545    1362      73
 
AvrII C'CTAG_G

Cuts at:   2815    2815 
   Size:       5980
 
BamHI G'GATC_C

Cuts at:   3310    3677    3310 
   Size:        367    5613
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2422    2982    3301    4756     618 
   Size:        325     217     627     635     560     319    1455    1842

  Fragments arranged by size: 

                1842    1455     635     627     560     325     319     217
            
 
BanII G_rGCy'C

Cuts at:   1391    2253    2520    3251    3299    1391 
   Size:        862     267     731      48    4072

  Fragments arranged by size: 

                4072     862     731     267      48
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2141    2905     928 
   Size:        971     242     764    4003

  Fragments arranged by size: 

                4003     971     764     242
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3112    3146    4171    4205    1903 
   Size:         34    1175      34    1025      34    3678

  Fragments arranged by size: 

                3678    1175    1025      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3872    5082    3872 
   Size:       1210    4770
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5980
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2052    3059    4591 
   Size:        107     122      71     631     605     380    1007    1532

Cuts at:   4591     136 
   Size:       1525

  Fragments arranged by size: 

                1532    1525    1007     631     605     380     122     107
                  71
 
BglII A'GATC_T

Cuts at:   3027    5975    3027 
   Size:       2948    3032
 
BlpI GC'TnA_GC

Cuts at:   3397    3397 
   Size:       5980
 
Bme1580I G_kGCm'C

Cuts at:   1790    3116    4041    5197    1790 
   Size:       1326     925    1156    2573

  Fragments arranged by size: 

                2573    1326    1156     925
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5980
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3058    3711    4669 
   Size:        416      53      83     399      24    1641     653     958

Cuts at:   4669     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1641     958     653     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5980
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2177    4640    1239 
   Size:        938    2463    2579

  Fragments arranged by size: 

                2579    2463     938
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2114    4105    4970    5268    2114 
   Size:       1991     865     298    2826

  Fragments arranged by size: 

                2826    1991     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2531    4643     882 
   Size:        617    1032    2112    2219

  Fragments arranged by size: 

                2219    2112    1032     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3771     493 
   Size:        711    2567    2702

  Fragments arranged by size: 

                2702    2567     711
 
BsaBI GATnn'nnATC

Cuts at:   5974    5974 
   Size:       5980
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3787    4169    4804     275 
   Size:         53      83     186     936    2254     382     635    1451

  Fragments arranged by size: 

                2254    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4413    4811    5085    5232    5643    4413 
   Size:        398     274     147     411    4750

  Fragments arranged by size: 

                4750     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2078    2108    2895    2925    1178 
   Size:         30     165      30     675      30     787      30    4233

  Fragments arranged by size: 

                4233     787     675     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2099    1186 
   Size:        913    5067
 
BseYI C'CCAG_C

Cuts at:   2050    2644    3141    5183    5679    2050 
   Size:        594     497    2042     496    2351

  Fragments arranged by size: 

                2351    2042     594     497     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2279    3182    1163 
   Size:        573     543     903    3961

  Fragments arranged by size: 

                3961     903     573     543
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2037    2448    3430    4191    5219    1540 
   Size:        275     186      36     411     982     761    1028    2301

  Fragments arranged by size: 

                2301    1028     982     761     411     275     186      36
            
 
BsiHKAI G_wGCw'C

Cuts at:   2705    3116    4041    4126    5197    2705 
   Size:        411     925      85    1071    3488

  Fragments arranged by size: 

                3488    1071     925     411      85
 
BsmI GAATG_Cn'

Cuts at:   2848    3491    3584    2848 
   Size:        643      93    5244

  Fragments arranged by size: 

                5244     643      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2253    2520    2705    3116    3251    3299    4041 
   Size:        399     463     267     185     411     135      48     742

Cuts at:   4041    4126    5197    1391 
   Size:         85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     463     411     399     267     185
                 135      85      48
 
BspEI T'CCGG_A

Cuts at:   5643    5643 
   Size:       5980
 
BspHI T'CATG_A

Cuts at:   3869    5599    3869 
   Size:       1730    4250
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2221    2301    2600    2673    3335     844 
   Size:       1377      80     299      73     662    3489

  Fragments arranged by size: 

                3489    1377     662     299      80      73
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3149    3867    1394 
   Size:        429    1326     718    3507

  Fragments arranged by size: 

                3507    1326     718     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4475    4649      65 
   Size:       1183    3227     174    1396

  Fragments arranged by size: 

                3227    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2223    2689    4624    4811    1700 
   Size:        110     413     466    1935     187    2869

  Fragments arranged by size: 

                2869    1935     466     413     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5980
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5980
 
BssSI C'ACGA_G

Cuts at:   2306    4040    5052    2306 
   Size:       1734    1012    3234

  Fragments arranged by size: 

                3234    1734    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5980
 
BstBI TT'CG_AA

Cuts at:   4784    4784 
   Size:       5980
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5980
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2215    2773    2975    2215 
   Size:        558     202    5220

  Fragments arranged by size: 

                5220     558     202
 
BstYI r'GATC_y

Cuts at:   1972    2439    3003    3027    3310    3677    4062    4079    5531 
   Size:        467     564      24     283     367     385      17    1452

Cuts at:   5531    5617    5629    5975    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     564     467     385     367     346     283
                  86      24      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2319     513 
   Size:        552    1254    4174

  Fragments arranged by size: 

                4174    1254     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2296     487 
   Size:        683      56    1070    4171

  Fragments arranged by size: 

                4171    1070     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2856    3224    3496    4290    4310    2856 
   Size:        368     272     794      20    4526

  Fragments arranged by size: 

                4526     794     368     272      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5980
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5945
 
DraI TTT'AAA

Cuts at:   3284    3284 
   Size:       5980
 
DraIII CAC_nnn'GTG

Cuts at:   2193    2193 
   Size:       5980
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4987     809 
   Size:        507    3671    1802

  Fragments arranged by size: 

                3671    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2221    2285    2445 
   Size:         54    1425      50     275      18     391      64     160

Cuts at:   2445    2988    3427    4316    5945       8 
   Size:        543     439     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     543     439     391     275     160
                  64      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2445    3427    1537 
   Size:        275     633     982    4090

  Fragments arranged by size: 

                4090     982     633     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2872    3910    1353 
   Size:       1519    1038    3423

  Fragments arranged by size: 

                3423    1519    1038
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4567    4941    5087     387 
   Size:       1064     138    2978     374     146    1280

  Fragments arranged by size: 

                2978    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2177    3044    4043    4640    5427    5695    1239 
   Size:        393     545     867     999     597     787     268    1524

  Fragments arranged by size: 

                1524     999     867     787     597     545     393     268
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2211    3232    2211 
   Size:       1021    4959
 
EcoRI G'AATT_C

Cuts at:   3289    3667    3289 
   Size:        378    5602
 
FspI TGC'GCA

Cuts at:   4486    4486 
   Size:       5980
 
HaeII r_GCGC'y

Cuts at:   1678    2047    2809    2940    2974    5127    1678 
   Size:        369     762     131      34    2153    2531

  Fragments arranged by size: 

                2531    2153     762     369     131      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2895    2927    4621    4653    4695 
   Size:         32     135      32    1323      32    1694      32      42

Cuts at:   4695    4727    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1694    1323     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2185    2836    3073    3102    3324    3570 
   Size:        988     281     247     651     237      29     222     246

Cuts at:   3570     669 
   Size:       3079

  Fragments arranged by size: 

                3079     988     651     281     247     246     237     222
                  29
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5980
 
HpaI GTT'AAC

Cuts at:   3570    3570 
   Size:       5980
 
KpnI G_GTAC'C

Cuts at:   3305    3305 
   Size:       5980
 
MfeI C'AATT_G

Cuts at:   3579    3579 
   Size:       5980
 
MluI A'CGCG_T

Cuts at:   5856    5856 
   Size:       5980
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5094    5278    1305 
   Size:       3789     184    2007

  Fragments arranged by size: 

                3789    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2287      10 
   Size:         54    1425     343     455    3703

  Fragments arranged by size: 

                3703    1425     455     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2771    3013    3122    3204    3242    3938    4297 
   Size:        648    1605     242     109      82      38     696     359

Cuts at:   4297    4456     518 
   Size:        159    2042

  Fragments arranged by size: 

                2042    1605     696     648     359     242     159     109
                  82      38
 
MspA1I CmG'CkG

Cuts at:   1606    2146    3264    3396    4073    5221    5466    1606 
   Size:        540    1118     132     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1118     677     540     245     132
 
NaeI GCC'GGC

Cuts at:   2691    2691 
   Size:       5980
 
NcoI C'CATG_G

Cuts at:    513    1065    2319     513 
   Size:        552    1254    4174

  Fragments arranged by size: 

                4174    1254     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5980
 
NgoMIV G'CCGG_C

Cuts at:   2689    2689 
   Size:       5980
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5980
 
NotI GC'GGCC_GC

Cuts at:   3427    3427 
   Size:       5980
 
NspI r_CATG'y

Cuts at:   1132    1229    3338    4883    1132 
   Size:         97    2109    1545    2229

  Fragments arranged by size: 

                2229    2109    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5980
 
PciI A'CATG_T

Cuts at:   4879    4879 
   Size:       5980
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2549    2602    1108 
   Size:       1441      53    4486

  Fragments arranged by size: 

                4486    1441      53
 
PmeI GTTT'AAAC

Cuts at:   3284    3284 
   Size:       5980
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4049    4081    5562    5594    4049 
   Size:         32    1481      32    4435

  Fragments arranged by size: 

                4435    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2211    3232    2211 
   Size:       1021    4959
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5980
 
PsiI TTA'TAA

Cuts at:   3550    3550 
   Size:       5980
 
PstI C_TGCA'G

Cuts at:    830    2670    3332     830 
   Size:       1840     662    3478

  Fragments arranged by size: 

                3478    1840     662
 
PvuI CG_AT'CG

Cuts at:   2001    2037    2001 
   Size:         36    5944
 
PvuII CAG'CTG

Cuts at:   2146    2146 
   Size:       5980
 
SalI G'TCGA_C

Cuts at:   1936    3322    1936 
   Size:       1386    4594
 
SbfI CC_TGCA'GG

Cuts at:   3332    3332 
   Size:       5980
 
ScaI AGT'ACT

Cuts at:   4228    5940    4228 
   Size:       1712    4268
 
SfcI C'TryA_G

Cuts at:    826    1045    2470    2666    3328    4463    5144    5335     826 
   Size:        219    1425     196     662    1135     681     191    1471

  Fragments arranged by size: 

                1471    1425    1135     681     662     219     196     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5980
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5980
 
SmaI CCC'GGG

Cuts at:   1872    3307    1872 
   Size:       1435    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2129    4084    4985    5247     820 
   Size:        197     926     186    1955     901     262    1553

  Fragments arranged by size: 

                1955    1553     926     901     262     197     186
 
SnaBI TAC'GTA

Cuts at:    493    3771     493 
   Size:       3278    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5980
 
SphI G_CATG'C

Cuts at:   1132    3338    1132 
   Size:       2206    3774
 
SspI AAT'ATT

Cuts at:      5      52    2530    3904       5 
   Size:         47    2478    1374    2081

  Fragments arranged by size: 

                2478    2081    1374      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5980
 
StyI C'CwwG_G

Cuts at:    513    1065    2319    2815    3419     513 
   Size:        552    1254     496     604    3074

  Fragments arranged by size: 

                3074    1254     604     552     496
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3058    3254    4007    4024    4177    4362    4807 
   Size:       1255    1478     196     753      17     153     185     445

Cuts at:   4807     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1478    1255     753     445     196     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3153    4226    5938 
   Size:        274      80      33      51    1443    1176    1073    1712

Cuts at:   5938      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1176    1073     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2078    2110    2868    2900    5940    5972    2078 
   Size:         32     758      32    3040      32    2086

  Fragments arranged by size: 

                3040    2086     758      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2360    3230    1316 
   Size:       1044     870    4066

  Fragments arranged by size: 

                4066    1044     870
 
XbaI T'CTAG_A

Cuts at:   3316    3316 
   Size:       5980
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5980
 
XmaI C'CCGG_G

Cuts at:   1870    3305    1870 
   Size:       1435    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3029    4109    3029 
   Size:       1080    4900
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3788     276 
   Size:         53      83     186     936    2254    2468

  Fragments arranged by size: 

                2468    2254     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbsI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI
    PshAI     PsiI     PstI     PvuI    PvuII     SalI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AloI     ApaI     AscI     BaeI     BbeI    BbvCI    BfrBI     BplI
   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I  EcoICRI    EcoNI    EcoRV
     FalI     FseI    FspAI     KasI     NarI     NruI     NsiI     PacI
     PfoI     PmlI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII
    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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