(Circular) (Six-base) MAPSORT of: pFN21AB8191.seq Check: 4218 from: 1 to: 5980 pFN21AB8191 5980 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2221 2221 Size: 5980 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3093 3323 1937 Size: 1156 230 4594 Fragments arranged by size: 4594 1156 230 Acc65I G'GTAC_C Cuts at: 3301 3301 Size: 5980 AclI AA'CG_TT Cuts at: 1563 4107 4480 1563 Size: 2544 373 3063 Fragments arranged by size: 3063 2544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3044 4043 5427 5695 1632 Size: 1412 999 1384 268 1917 Fragments arranged by size: 1917 1412 1384 999 268 AfeI AGC'GCT Cuts at: 2807 2938 2972 2807 Size: 131 34 5815 Fragments arranged by size: 5815 131 34 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5783 AflIII A'CryG_T Cuts at: 1203 4879 5856 1203 Size: 3676 977 1327 Fragments arranged by size: 3676 1327 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 4709 4709 Size: 5980 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5980 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5980 AlwNI CAG_nnn'CTG Cuts at: 3723 5295 3723 Size: 1572 4408 ApaLI G'TGCA_C Cuts at: 3112 4037 5193 3112 Size: 925 1156 3899 Fragments arranged by size: 3899 1156 925 ApoI r'AATT_y Cuts at: 1493 3289 3515 3667 5776 1493 Size: 1796 226 152 2109 1697 Fragments arranged by size: 2109 1796 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 3305 1870 Size: 73 1362 4545 Fragments arranged by size: 4545 1362 73 AvrII C'CTAG_G Cuts at: 2815 2815 Size: 5980 BamHI G'GATC_C Cuts at: 3310 3677 3310 Size: 367 5613 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2422 2982 3301 4756 618 Size: 325 217 627 635 560 319 1455 1842 Fragments arranged by size: 1842 1455 635 627 560 325 319 217 BanII G_rGCy'C Cuts at: 1391 2253 2520 3251 3299 1391 Size: 862 267 731 48 4072 Fragments arranged by size: 4072 862 731 267 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2141 2905 928 Size: 971 242 764 4003 Fragments arranged by size: 4003 971 764 242 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3112 3146 4171 4205 1903 Size: 34 1175 34 1025 34 3678 Fragments arranged by size: 3678 1175 1025 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3872 5082 3872 Size: 1210 4770 BclI T'GATC_A Cuts at: 1548 1548 Size: 5980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 3059 4591 Size: 107 122 71 631 605 380 1007 1532 Cuts at: 4591 136 Size: 1525 Fragments arranged by size: 1532 1525 1007 631 605 380 122 107 71 BglII A'GATC_T Cuts at: 3027 5975 3027 Size: 2948 3032 BlpI GC'TnA_GC Cuts at: 3397 3397 Size: 5980 Bme1580I G_kGCm'C Cuts at: 1790 3116 4041 5197 1790 Size: 1326 925 1156 2573 Fragments arranged by size: 2573 1326 1156 925 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3058 3711 4669 Size: 416 53 83 399 24 1641 653 958 Cuts at: 4669 442 Size: 1753 Fragments arranged by size: 1753 1641 958 653 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2177 4640 1239 Size: 938 2463 2579 Fragments arranged by size: 2579 2463 938 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2114 4105 4970 5268 2114 Size: 1991 865 298 2826 Fragments arranged by size: 2826 1991 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2531 4643 882 Size: 617 1032 2112 2219 Fragments arranged by size: 2219 2112 1032 617 BsaAI yAC'GTr Cuts at: 493 1204 3771 493 Size: 711 2567 2702 Fragments arranged by size: 2702 2567 711 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3787 4169 4804 275 Size: 53 83 186 936 2254 382 635 1451 Fragments arranged by size: 2254 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4413 4811 5085 5232 5643 4413 Size: 398 274 147 411 4750 Fragments arranged by size: 4750 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2108 2895 2925 1178 Size: 30 165 30 675 30 787 30 4233 Fragments arranged by size: 4233 787 675 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 1186 Size: 913 5067 BseYI C'CCAG_C Cuts at: 2050 2644 3141 5183 5679 2050 Size: 594 497 2042 496 2351 Fragments arranged by size: 2351 2042 594 497 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2279 3182 1163 Size: 573 543 903 3961 Fragments arranged by size: 3961 903 573 543 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2037 2448 3430 4191 5219 1540 Size: 275 186 36 411 982 761 1028 2301 Fragments arranged by size: 2301 1028 982 761 411 275 186 36 BsiHKAI G_wGCw'C Cuts at: 2705 3116 4041 4126 5197 2705 Size: 411 925 85 1071 3488 Fragments arranged by size: 3488 1071 925 411 85 BsmI GAATG_Cn' Cuts at: 2848 3491 3584 2848 Size: 643 93 5244 Fragments arranged by size: 5244 643 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2253 2520 2705 3116 3251 3299 4041 Size: 399 463 267 185 411 135 48 742 Cuts at: 4041 4126 5197 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 463 411 399 267 185 135 85 48 BspEI T'CCGG_A Cuts at: 5643 5643 Size: 5980 BspHI T'CATG_A Cuts at: 3869 5599 3869 Size: 1730 4250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2221 2301 2600 2673 3335 844 Size: 1377 80 299 73 662 3489 Fragments arranged by size: 3489 1377 662 299 80 73 BsrBI CCG'CTC Cuts at: 1394 1823 3149 3867 1394 Size: 429 1326 718 3507 Fragments arranged by size: 3507 1326 718 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4475 4649 65 Size: 1183 3227 174 1396 Fragments arranged by size: 3227 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2223 2689 4624 4811 1700 Size: 110 413 466 1935 187 2869 Fragments arranged by size: 2869 1935 466 413 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5980 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 2306 4040 5052 2306 Size: 1734 1012 3234 Fragments arranged by size: 3234 1734 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5980 BstBI TT'CG_AA Cuts at: 4784 4784 Size: 5980 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5980 BstXI CCAn_nnnn'nTGG Cuts at: 2215 2773 2975 2215 Size: 558 202 5220 Fragments arranged by size: 5220 558 202 BstYI r'GATC_y Cuts at: 1972 2439 3003 3027 3310 3677 4062 4079 5531 Size: 467 564 24 283 367 385 17 1452 Cuts at: 5531 5617 5629 5975 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 564 467 385 367 346 283 86 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2319 513 Size: 552 1254 4174 Fragments arranged by size: 4174 1254 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2296 487 Size: 683 56 1070 4171 Fragments arranged by size: 4171 1070 683 56 BtsI GCAGTG_nn' Cuts at: 2856 3224 3496 4290 4310 2856 Size: 368 272 794 20 4526 Fragments arranged by size: 4526 794 368 272 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5945 DraI TTT'AAA Cuts at: 3284 3284 Size: 5980 DraIII CAC_nnn'GTG Cuts at: 2193 2193 Size: 5980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4987 809 Size: 507 3671 1802 Fragments arranged by size: 3671 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2221 2285 2445 Size: 54 1425 50 275 18 391 64 160 Cuts at: 2445 2988 3427 4316 5945 8 Size: 543 439 889 1629 43 Fragments arranged by size: 1629 1425 889 543 439 391 275 160 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2445 3427 1537 Size: 275 633 982 4090 Fragments arranged by size: 4090 982 633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2872 3910 1353 Size: 1519 1038 3423 Fragments arranged by size: 3423 1519 1038 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4567 4941 5087 387 Size: 1064 138 2978 374 146 1280 Fragments arranged by size: 2978 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 3044 4043 4640 5427 5695 1239 Size: 393 545 867 999 597 787 268 1524 Fragments arranged by size: 1524 999 867 787 597 545 393 268 EcoO109I rG'GnC_Cy Cuts at: 2211 3232 2211 Size: 1021 4959 EcoRI G'AATT_C Cuts at: 3289 3667 3289 Size: 378 5602 FspI TGC'GCA Cuts at: 4486 4486 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 2047 2809 2940 2974 5127 1678 Size: 369 762 131 34 2153 2531 Fragments arranged by size: 2531 2153 762 369 131 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2895 2927 4621 4653 4695 Size: 32 135 32 1323 32 1694 32 42 Cuts at: 4695 4727 1373 Size: 32 2626 Fragments arranged by size: 2626 1694 1323 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2185 2836 3073 3102 3324 3570 Size: 988 281 247 651 237 29 222 246 Cuts at: 3570 669 Size: 3079 Fragments arranged by size: 3079 988 651 281 247 246 237 222 29 HindIII A'AGCT_T Cuts at: 748 748 Size: 5980 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KpnI G_GTAC'C Cuts at: 3305 3305 Size: 5980 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5094 5278 1305 Size: 3789 184 2007 Fragments arranged by size: 3789 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2287 10 Size: 54 1425 343 455 3703 Fragments arranged by size: 3703 1425 455 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2771 3013 3122 3204 3242 3938 4297 Size: 648 1605 242 109 82 38 696 359 Cuts at: 4297 4456 518 Size: 159 2042 Fragments arranged by size: 2042 1605 696 648 359 242 159 109 82 38 MspA1I CmG'CkG Cuts at: 1606 2146 3264 3396 4073 5221 5466 1606 Size: 540 1118 132 677 1148 245 2120 Fragments arranged by size: 2120 1148 1118 677 540 245 132 NaeI GCC'GGC Cuts at: 2691 2691 Size: 5980 NcoI C'CATG_G Cuts at: 513 1065 2319 513 Size: 552 1254 4174 Fragments arranged by size: 4174 1254 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NgoMIV G'CCGG_C Cuts at: 2689 2689 Size: 5980 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5980 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 3338 4883 1132 Size: 97 2109 1545 2229 Fragments arranged by size: 2229 2109 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5980 PciI A'CATG_T Cuts at: 4879 4879 Size: 5980 PflMI CCAn_nnn'nTGG Cuts at: 1108 2549 2602 1108 Size: 1441 53 4486 Fragments arranged by size: 4486 1441 53 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2211 3232 2211 Size: 1021 4959 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5980 PsiI TTA'TAA Cuts at: 3550 3550 Size: 5980 PstI C_TGCA'G Cuts at: 830 2670 3332 830 Size: 1840 662 3478 Fragments arranged by size: 3478 1840 662 PvuI CG_AT'CG Cuts at: 2001 2037 2001 Size: 36 5944 PvuII CAG'CTG Cuts at: 2146 2146 Size: 5980 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SfcI C'TryA_G Cuts at: 826 1045 2470 2666 3328 4463 5144 5335 826 Size: 219 1425 196 662 1135 681 191 1471 Fragments arranged by size: 1471 1425 1135 681 662 219 196 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 3307 1872 Size: 1435 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2129 4084 4985 5247 820 Size: 197 926 186 1955 901 262 1553 Fragments arranged by size: 1955 1553 926 901 262 197 186 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3338 1132 Size: 2206 3774 SspI AAT'ATT Cuts at: 5 52 2530 3904 5 Size: 47 2478 1374 2081 Fragments arranged by size: 2478 2081 1374 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5980 StyI C'CwwG_G Cuts at: 513 1065 2319 2815 3419 513 Size: 552 1254 496 604 3074 Fragments arranged by size: 3074 1254 604 552 496 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3058 3254 4007 4024 4177 4362 4807 Size: 1255 1478 196 753 17 153 185 445 Cuts at: 4807 325 Size: 1498 Fragments arranged by size: 1498 1478 1255 753 445 196 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3153 4226 5938 Size: 274 80 33 51 1443 1176 1073 1712 Cuts at: 5938 96 Size: 138 Fragments arranged by size: 1712 1443 1176 1073 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2078 2110 2868 2900 5940 5972 2078 Size: 32 758 32 3040 32 2086 Fragments arranged by size: 3040 2086 758 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2360 3230 1316 Size: 1044 870 4066 Fragments arranged by size: 4066 1044 870 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5980 XmaI C'CCGG_G Cuts at: 1870 3305 1870 Size: 1435 4545 XmnI GAAnn'nnTTC Cuts at: 3029 4109 3029 Size: 1080 4900 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI