(Circular) (Six-base) MAPSORT of: pFN21AB8187.seq Check: 2680 from: 1 to: 6061 pFN21AB8187 6061 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2794 3070 2794 Size: 276 5785 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3404 1937 Size: 1467 4594 Acc65I G'GTAC_C Cuts at: 3172 3382 3172 Size: 210 5851 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2690 3120 4124 5508 5776 1632 Size: 1058 430 1004 1384 268 1917 Fragments arranged by size: 1917 1384 1058 1004 430 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 3126 4960 5937 1203 Size: 1923 1834 977 1327 Fragments arranged by size: 1923 1834 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 2388 3804 5376 2388 Size: 1416 1572 3073 Fragments arranged by size: 3073 1572 1416 ApaI G_GGCC'C Cuts at: 2377 2377 Size: 6061 ApaLI G'TGCA_C Cuts at: 4118 5274 4118 Size: 1156 4905 ApoI r'AATT_y Cuts at: 1493 3317 3370 3596 3748 5857 1493 Size: 1824 53 226 152 2109 1697 Fragments arranged by size: 2109 1824 1697 226 152 53 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 3386 1870 Size: 73 1443 4545 Fragments arranged by size: 4545 1443 73 BamHI G'GATC_C Cuts at: 2828 3391 3758 2828 Size: 563 367 5131 Fragments arranged by size: 5131 563 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2428 2768 2855 3172 3382 Size: 325 217 627 641 340 87 317 210 Cuts at: 3382 4837 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 641 627 340 325 317 217 210 87 BanII G_rGCy'C Cuts at: 1391 2377 2943 3380 1391 Size: 986 566 437 4072 Fragments arranged by size: 4072 986 566 437 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3108 3303 928 Size: 971 1209 195 3686 Fragments arranged by size: 3686 1209 971 195 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2026 2060 2845 2879 4252 4286 1903 Size: 34 89 34 785 34 1373 34 3678 Fragments arranged by size: 3678 1373 785 89 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 2163 1548 Size: 615 5446 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2034 2136 4672 Size: 107 122 71 631 605 362 102 2536 Cuts at: 4672 136 Size: 1525 Fragments arranged by size: 2536 1525 631 605 362 122 107 102 71 BglII A'GATC_T Cuts at: 3282 6056 3282 Size: 2774 3287 BlpI GC'TnA_GC Cuts at: 3118 3478 3118 Size: 360 5701 Bme1580I G_kGCm'C Cuts at: 1790 2179 2313 2377 4122 5278 1790 Size: 389 134 64 1745 1156 2573 Fragments arranged by size: 2573 1745 1156 389 134 64 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2131 2753 3792 Size: 416 53 83 399 24 714 622 1039 Cuts at: 3792 4750 442 Size: 958 1753 Fragments arranged by size: 1753 1039 958 714 622 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2158 2462 2629 2158 Size: 304 167 5590 Fragments arranged by size: 5590 304 167 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2637 2704 4721 1239 Size: 1398 67 2017 2579 Fragments arranged by size: 2579 2017 1398 67 Bpu10I CC'TnA_GC Cuts at: 2729 2729 Size: 6061 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3362 4186 5051 5349 3362 Size: 824 865 298 4074 Fragments arranged by size: 4074 865 824 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4724 882 Size: 617 3225 2219 Fragments arranged by size: 3225 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3852 493 Size: 711 2648 2702 Fragments arranged by size: 2702 2648 711 BsaBI GATnn'nnATC Cuts at: 2162 6055 2162 Size: 3893 2168 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3868 4250 4885 275 Size: 53 83 186 936 2335 382 635 1451 Fragments arranged by size: 2335 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4494 4892 5166 5313 5724 4494 Size: 398 274 147 411 4831 Fragments arranged by size: 4831 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5836 Fragments arranged by size: 5836 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2781 1186 Size: 1595 4466 BseYI C'CCAG_C Cuts at: 5264 5760 5264 Size: 496 5565 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 1163 Size: 573 564 4924 Fragments arranged by size: 4924 573 564 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4272 5300 1540 Size: 275 186 1510 761 1028 2301 Fragments arranged by size: 2301 1510 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2757 4122 4207 5278 2757 Size: 1365 85 1071 3540 Fragments arranged by size: 3540 1365 1071 85 BsmI GAATG_Cn' Cuts at: 3572 3665 3572 Size: 93 5968 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2179 2313 2377 2757 2943 3380 4122 Size: 399 389 134 64 380 186 437 742 Cuts at: 4122 4207 5278 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 437 399 389 380 186 134 85 64 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 3276 3950 5680 3276 Size: 674 1730 3657 Fragments arranged by size: 3657 1730 674 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2794 3070 3416 844 Size: 1950 276 346 3489 Fragments arranged by size: 3489 1950 346 276 BsrBI CCG'CTC Cuts at: 1394 1823 2142 2455 3948 1394 Size: 429 319 313 1493 3507 Fragments arranged by size: 3507 1493 429 319 313 BsrDI GCAATG_nn' Cuts at: 65 1248 2653 4556 4730 65 Size: 1183 1405 1903 174 1396 Fragments arranged by size: 1903 1405 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4705 4892 1700 Size: 110 2895 187 2869 Fragments arranged by size: 2895 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2251 96 Size: 2155 3906 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 4121 5133 4121 Size: 1012 5049 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6061 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstXI CCAn_nnnn'nTGG Cuts at: 3044 3044 Size: 6061 BstYI r'GATC_y Cuts at: 1972 2828 3282 3391 3758 4143 4160 5612 5698 Size: 856 454 109 367 385 17 1452 86 Cuts at: 5698 5710 6056 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 856 454 385 367 346 109 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2197 513 Size: 552 939 193 4377 Fragments arranged by size: 4377 939 552 193 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 2295 3147 3577 4371 4391 2295 Size: 852 430 794 20 3965 Fragments arranged by size: 3965 852 794 430 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DraIII CAC_nnn'GTG Cuts at: 2025 2025 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3508 4397 6026 Size: 54 1425 50 275 18 1678 889 1629 Cuts at: 6026 8 Size: 43 Fragments arranged by size: 1678 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2690 2709 3991 1353 Size: 1337 19 1282 3423 Fragments arranged by size: 3423 1337 1282 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4648 5022 5168 387 Size: 1064 138 3059 374 146 1280 Fragments arranged by size: 3059 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2637 2690 2704 3120 4124 4721 5508 Size: 393 1005 53 14 416 1004 597 787 Cuts at: 5508 5776 1239 Size: 268 1524 Fragments arranged by size: 1524 1005 1004 787 597 416 393 268 53 14 EcoNI CCTnn'n_nnAGG Cuts at: 2087 2087 Size: 6061 EcoO109I rG'GnC_Cy Cuts at: 2356 2373 2374 2356 Size: 17 1 6043 Fragments arranged by size: 6043 17 1 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 5208 1678 Size: 3530 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2946 2978 4702 4734 4776 Size: 32 135 32 1374 32 1724 32 42 Cuts at: 4776 4808 1373 Size: 32 2626 Fragments arranged by size: 2626 1724 1374 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KpnI G_GTAC'C Cuts at: 3176 3386 3176 Size: 210 5851 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5175 5359 1305 Size: 3870 184 2007 Fragments arranged by size: 3870 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4239 Fragments arranged by size: 4239 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2304 2657 2748 3329 4019 4378 4537 Size: 648 1138 353 91 581 690 359 159 Cuts at: 4537 518 Size: 2042 Fragments arranged by size: 2042 1138 690 648 581 359 353 159 91 MspA1I CmG'CkG Cuts at: 1606 2637 3477 4154 5302 5547 1606 Size: 1031 840 677 1148 245 2120 Fragments arranged by size: 2120 1148 1031 840 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4570 Fragments arranged by size: 4570 939 552 NdeI CA'TA_TG Cuts at: 387 2824 387 Size: 2437 3624 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 2960 3055 3130 3419 4964 1132 Size: 97 1731 95 75 289 1545 2229 Fragments arranged by size: 2229 1731 1545 289 97 95 75 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6061 PciI A'CATG_T Cuts at: 3126 4960 3126 Size: 1834 4227 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6061 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2543 2575 4130 4162 5643 5675 2543 Size: 32 1555 32 1481 32 2929 Fragments arranged by size: 2929 1555 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2356 2356 Size: 6061 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PspOMI G'GGCC_C Cuts at: 2373 2373 Size: 6061 PstI C_TGCA'G Cuts at: 830 3090 3413 830 Size: 2260 323 3478 Fragments arranged by size: 3478 2260 323 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SapI GCTCTTCn'nnn_ Cuts at: 2690 2690 Size: 6061 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SexAI A'CCwGG_T Cuts at: 2358 3247 2358 Size: 889 5172 SfcI C'TryA_G Cuts at: 826 1045 3086 3409 4544 5225 5416 826 Size: 219 2041 323 1135 681 191 1471 Fragments arranged by size: 2041 1471 1135 681 323 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3341 4165 5066 5328 820 Size: 197 926 1398 824 901 262 1553 Fragments arranged by size: 1553 1398 926 901 824 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 3228 1517 Size: 1711 4350 StyI C'CwwG_G Cuts at: 513 1065 2004 2424 3500 513 Size: 552 939 420 1076 3074 Fragments arranged by size: 3074 1076 939 552 420 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4088 4105 4258 4443 4888 325 Size: 1255 2508 17 153 185 445 1498 Fragments arranged by size: 2508 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2251 2268 2687 Size: 274 80 33 51 1443 274 17 419 Cuts at: 2687 2842 4307 6019 96 Size: 155 1465 1712 138 Fragments arranged by size: 1712 1465 1443 419 274 274 155 138 80 51 33 17 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3198 3230 6021 6053 3198 Size: 32 2791 32 3206 Fragments arranged by size: 3206 2791 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2654 1316 Size: 1338 4723 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2866 2866 Size: 6061 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 3228 4190 3228 Size: 962 5099 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI