(Circular) (Six-base) MAPSORT of: pFN21AB8185.seq Check: 4661 from: 1 to: 6088 pFN21AB8185 6088 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3431 1937 Size: 1494 4594 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2660 3155 4151 5535 5803 1632 Size: 1028 495 996 1384 268 1917 Fragments arranged by size: 1917 1384 1028 996 495 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 4987 5964 1203 Size: 3784 977 1327 Fragments arranged by size: 3784 1327 977 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 2794 3831 5403 2794 Size: 1037 1572 3479 Fragments arranged by size: 3479 1572 1037 ApaLI G'TGCA_C Cuts at: 4145 5301 4145 Size: 1156 4932 ApoI r'AATT_y Cuts at: 1493 3397 3623 3775 5884 1493 Size: 1904 226 152 2109 1697 Fragments arranged by size: 2109 1904 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 3413 1870 Size: 73 1470 4545 Fragments arranged by size: 4545 1470 73 BamHI G'GATC_C Cuts at: 3418 3785 3418 Size: 367 5721 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2565 2738 3409 4864 618 Size: 325 217 627 778 173 671 1455 1842 Fragments arranged by size: 1842 1455 778 671 627 325 217 173 BanII G_rGCy'C Cuts at: 1391 3407 1391 Size: 2016 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2666 2943 928 Size: 971 767 277 4073 Fragments arranged by size: 4073 971 767 277 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4279 4313 1903 Size: 34 2342 34 3678 Fragments arranged by size: 3678 2342 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 1548 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2381 4699 136 Size: 107 122 71 631 605 709 2318 1525 Fragments arranged by size: 2318 1525 709 631 605 122 107 71 BglII A'GATC_T Cuts at: 6083 6083 Size: 6088 BlpI GC'TnA_GC Cuts at: 3505 3505 Size: 6088 Bme1580I G_kGCm'C Cuts at: 1790 2331 2432 2741 4149 5305 1790 Size: 541 101 309 1408 1156 2573 Fragments arranged by size: 2573 1408 1156 541 309 101 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6088 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3819 4777 442 Size: 416 53 83 399 24 2402 958 1753 Fragments arranged by size: 2402 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2259 2307 2697 3137 3388 4748 1239 Size: 1020 48 390 440 251 1360 2579 Fragments arranged by size: 2579 1360 1020 440 390 251 48 Bpu10I CC'TnA_GC Cuts at: 2099 2099 Size: 6088 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2558 2633 2651 3299 4213 5078 5376 2558 Size: 75 18 648 914 865 298 3270 Fragments arranged by size: 3270 914 865 648 298 75 18 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4751 882 Size: 617 3252 2219 Fragments arranged by size: 3252 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3879 493 Size: 711 2675 2702 Fragments arranged by size: 2702 2675 711 BsaBI GATnn'nnATC Cuts at: 3167 6082 3167 Size: 2915 3173 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3895 4277 4912 275 Size: 53 83 186 936 2362 382 635 1451 Fragments arranged by size: 2362 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4521 4919 5193 5340 5751 4521 Size: 398 274 147 411 4858 Fragments arranged by size: 4858 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2953 2983 1178 Size: 30 165 30 1550 30 4283 Fragments arranged by size: 4283 1550 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6088 BseYI C'CCAG_C Cuts at: 2330 2394 2557 5291 5787 2330 Size: 64 163 2734 496 2631 Fragments arranged by size: 2734 2631 496 163 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3332 1163 Size: 573 1596 3919 Fragments arranged by size: 3919 1596 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2659 3538 4299 5327 1540 Size: 275 186 658 879 761 1028 2301 Fragments arranged by size: 2301 1028 879 761 658 275 186 BsiHKAI G_wGCw'C Cuts at: 4149 4234 5305 4149 Size: 85 1071 4932 Fragments arranged by size: 4932 1071 85 BsmI GAATG_Cn' Cuts at: 3599 3692 3599 Size: 93 5995 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2331 2432 2741 3407 4149 4234 5305 Size: 399 541 101 309 666 742 85 1071 Cuts at: 5305 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 666 541 399 309 101 85 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 2868 3977 5707 2868 Size: 1109 1730 3249 Fragments arranged by size: 3249 1730 1109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3443 844 Size: 2599 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2188 3147 3304 3975 1394 Size: 429 365 959 157 671 3507 Fragments arranged by size: 3507 959 671 429 365 157 BsrDI GCAATG_nn' Cuts at: 65 1248 2615 2725 4583 4757 65 Size: 1183 1367 110 1858 174 1396 Fragments arranged by size: 1858 1396 1367 1183 174 110 BsrFI r'CCGG_y Cuts at: 1700 1810 2562 4732 4919 1700 Size: 110 752 2170 187 2869 Fragments arranged by size: 2869 2170 752 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 4148 5160 4148 Size: 1012 5076 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6088 BstBI TT'CG_AA Cuts at: 4892 4892 Size: 6088 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstYI r'GATC_y Cuts at: 1972 2094 2205 3120 3418 3785 4170 4187 5639 Size: 122 111 915 298 367 385 17 1452 Cuts at: 5639 5725 5737 6083 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 915 385 367 346 298 122 111 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2579 513 Size: 552 939 575 4022 Fragments arranged by size: 4022 939 575 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5349 Fragments arranged by size: 5349 683 56 BtsI GCAGTG_nn' Cuts at: 2391 3604 4398 4418 2391 Size: 1213 794 20 4061 Fragments arranged by size: 4061 1213 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DraIII CAC_nnn'GTG Cuts at: 2902 2902 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2522 5095 809 Size: 507 1206 2573 1802 Fragments arranged by size: 2573 1802 1206 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2119 2656 Size: 54 1425 50 275 18 258 31 537 Cuts at: 2656 2805 3295 3535 4424 6053 8 Size: 149 490 240 889 1629 43 Fragments arranged by size: 1629 1425 889 537 490 275 258 240 149 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 2656 3535 1537 Size: 275 844 879 4090 Fragments arranged by size: 4090 879 844 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2864 4018 1353 Size: 1511 1154 3423 Fragments arranged by size: 3423 1511 1154 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2071 4675 5049 5195 387 Size: 1064 138 435 47 2604 374 146 1280 Fragments arranged by size: 2604 1280 1064 435 374 146 138 47 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2259 2307 2660 2697 3137 3155 3388 Size: 393 627 48 353 37 440 18 233 Cuts at: 3388 4151 4748 5535 5803 1239 Size: 763 597 787 268 1524 Fragments arranged by size: 1524 787 763 627 597 440 393 353 268 233 48 37 18 EcoO109I rG'GnC_Cy Cuts at: 2259 3048 2259 Size: 789 5299 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 2983 5235 1678 Size: 1305 2252 2531 Fragments arranged by size: 2531 2252 1305 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2426 2458 2912 2944 3200 Size: 32 135 32 854 32 454 32 256 Cuts at: 3200 3232 4729 4761 4803 4835 1373 Size: 32 1497 32 42 32 2626 Fragments arranged by size: 2626 1497 854 454 256 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3432 3678 669 Size: 988 281 1494 246 3079 Fragments arranged by size: 3079 1494 988 281 246 HindIII A'AGCT_T Cuts at: 748 2126 748 Size: 1378 4710 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3120 5202 5386 1305 Size: 1815 2082 184 2007 Fragments arranged by size: 2082 2007 1815 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2807 3297 10 Size: 54 1425 343 975 490 2801 Fragments arranged by size: 2801 1425 975 490 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2531 4046 4405 4564 518 Size: 648 1365 1515 359 159 2042 Fragments arranged by size: 2042 1515 1365 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3504 4181 5329 5574 1606 Size: 1898 677 1148 245 2120 Fragments arranged by size: 2120 1898 1148 677 245 NaeI GCC'GGC Cuts at: 2564 2564 Size: 6088 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4597 Fragments arranged by size: 4597 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NgoMIV G'CCGG_C Cuts at: 2562 2562 Size: 6088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 3446 4991 1132 Size: 97 2217 1545 2229 Fragments arranged by size: 2229 2217 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 2902 3366 1108 Size: 1794 464 3830 Fragments arranged by size: 3830 1794 464 PfoI T'CCnGG_A Cuts at: 3114 3114 Size: 6088 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2259 3048 2259 Size: 789 5299 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PstI C_TGCA'G Cuts at: 830 3228 3440 830 Size: 2398 212 3478 Fragments arranged by size: 3478 2398 212 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SanDI GG'GwC_CC Cuts at: 2259 3048 2259 Size: 789 5299 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 3224 3436 4571 5252 5443 826 Size: 219 2179 212 1135 681 191 1471 Fragments arranged by size: 2179 1471 1135 681 219 212 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2573 2648 2666 3314 4192 5093 Size: 197 926 630 75 18 648 878 901 Cuts at: 5093 5355 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 878 648 630 262 197 75 18 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3446 1132 Size: 2314 3774 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6088 StyI C'CwwG_G Cuts at: 513 1065 2004 2916 3073 3360 3527 513 Size: 552 939 912 157 287 167 3074 Fragments arranged by size: 3074 939 912 552 287 167 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2164 4115 4132 4285 4470 4915 325 Size: 1255 584 1951 17 153 185 445 1498 Fragments arranged by size: 1951 1498 1255 584 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2835 3261 4334 Size: 274 80 33 51 1443 858 426 1073 Cuts at: 4334 6046 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1073 858 426 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6048 6080 6048 Size: 32 6056 Tth111I GACn'n_nGTC Cuts at: 1316 2522 1316 Size: 1206 4882 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6088 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 2129 4217 2129 Size: 2088 4000 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI