Restriction map for
pFN21AB8185
(Linear) (Six-base) MAPSORT of: KIBB8185.seq Check: 4187 from: 1 to: 1383
KIBB8185.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 1150 1383
Size: 655 495 233
Fragments arranged by size:
655 495 233
AlwNI CAG_nnn'CTG
Cuts at: 0 789 1383
Size: 789 594
BanI G'GyrC_C
Cuts at: 0 560 733 1383
Size: 560 173 650
Fragments arranged by size:
650 560 173
BbsI GAAGACnn'nnnn_
Cuts at: 0 661 938 1383
Size: 661 277 445
Fragments arranged by size:
661 445 277
BglI GCCn_nnn'nGGC
Cuts at: 0 376 1383
Size: 376 1007
Bme1580I G_kGCm'C
Cuts at: 0 326 427 736 1383
Size: 326 101 309 647
Fragments arranged by size:
647 326 309 101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 302 692 1132 1383
Size: 254 48 390 440 251
Fragments arranged by size:
440 390 254 251 48
Bpu10I CC'TnA_GC
Cuts at: 0 94 1383
Size: 94 1289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 628 646 1294 1383
Size: 553 75 18 648 89
Fragments arranged by size:
648 553 89 75 18
BsaBI GATnn'nnATC
Cuts at: 0 1162 1383
Size: 1162 221
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 948 978 1383
Size: 948 30 405
Fragments arranged by size:
948 405 30
BseYI C'CCAG_C
Cuts at: 0 325 389 552 1383
Size: 325 64 163 831
Fragments arranged by size:
831 325 163 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1327 1383
Size: 1327 56
BsiEI CG_ry'CG
Cuts at: 0 654 1383
Size: 654 729
Bsp1286I G_dGCh'C
Cuts at: 0 326 427 736 1383
Size: 326 101 309 647
Fragments arranged by size:
647 326 309 101
BspHI T'CATG_A
Cuts at: 0 863 1383
Size: 863 520
BsrBI CCG'CTC
Cuts at: 0 183 1142 1299 1383
Size: 183 959 157 84
Fragments arranged by size:
959 183 157 84
BsrDI GCAATG_nn'
Cuts at: 0 610 720 1383
Size: 610 110 663
Fragments arranged by size:
663 610 110
BsrFI r'CCGG_y
Cuts at: 0 557 1383
Size: 557 826
BstYI r'GATC_y
Cuts at: 0 89 200 1115 1383
Size: 89 111 915 268
Fragments arranged by size:
915 268 111 89
BtgI C'CryG_G
Cuts at: 0 574 1383
Size: 574 809
BtsI GCAGTG_nn'
Cuts at: 0 386 1383
Size: 386 997
DraIII CAC_nnn'GTG
Cuts at: 0 897 1383
Size: 897 486
DrdI GACnn_nn'nnGTC
Cuts at: 0 517 1383
Size: 517 866
EaeI y'GGCC_r
Cuts at: 0 83 114 651 800 1290 1383
Size: 83 31 537 149 490 93
Fragments arranged by size:
537 490 149 93 83 31
EagI C'GGCC_G
Cuts at: 0 651 1383
Size: 651 732
EarI CTCTTCn'nnn_
Cuts at: 0 859 1383
Size: 859 524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 66 1383
Size: 19 47 1317
Fragments arranged by size:
1317 47 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 302 655 692 1132 1150 1383
Size: 254 48 353 37 440 18 233
Fragments arranged by size:
440 353 254 233 48 37 18
EcoO109I rG'GnC_Cy
Cuts at: 0 254 1043 1383
Size: 254 789 340
Fragments arranged by size:
789 340 254
HaeII r_GCGC'y
Cuts at: 0 978 1383
Size: 978 405
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 421 453 907 939 1195 1227 1383
Size: 421 32 454 32 256 32 156
Fragments arranged by size:
454 421 256 156 32 32 32
HindIII A'AGCT_T
Cuts at: 0 121 1383
Size: 121 1262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1115 1383
Size: 1115 268
MscI TGG'CCA
Cuts at: 0 802 1292 1383
Size: 802 490 91
Fragments arranged by size:
802 490 91
MslI CAynn'nnrTG
Cuts at: 0 526 1383
Size: 526 857
NaeI GCC'GGC
Cuts at: 0 559 1383
Size: 559 824
NgoMIV G'CCGG_C
Cuts at: 0 557 1383
Size: 557 826
PflMI CCAn_nnn'nTGG
Cuts at: 0 897 1361 1383
Size: 897 464 22
Fragments arranged by size:
897 464 22
PfoI T'CCnGG_A
Cuts at: 0 1109 1383
Size: 1109 274
PpuMI rG'GwC_Cy
Cuts at: 0 254 1043 1383
Size: 254 789 340
Fragments arranged by size:
789 340 254
PstI C_TGCA'G
Cuts at: 0 1223 1383
Size: 1223 160
SanDI GG'GwC_CC
Cuts at: 0 254 1043 1383
Size: 254 789 340
Fragments arranged by size:
789 340 254
SfcI C'TryA_G
Cuts at: 0 1219 1383
Size: 1219 164
SmlI C'TyrA_G
Cuts at: 0 568 643 661 1309 1383
Size: 568 75 18 648 74
Fragments arranged by size:
648 568 75 74 18
StyI C'CwwG_G
Cuts at: 0 911 1068 1355 1383
Size: 911 157 287 28
Fragments arranged by size:
911 287 157 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 159 1383
Size: 159 1224
TatI w'GTAC_w
Cuts at: 0 830 1256 1383
Size: 830 426 127
Fragments arranged by size:
830 426 127
Tth111I GACn'n_nGTC
Cuts at: 0 517 1383
Size: 517 866
XmnI GAAnn'nnTTC
Cuts at: 0 124 1383
Size: 124 1259
Enzymes that do cut:
AcuI AlwNI BanI BbsI BglI Bme1580I BpmI Bpu10I
BpuEI BsaBI BsaXI BseYI BsgI BsiEI Bsp1286I BspHI
BsrBI BsrDI BsrFI BstYI BtgI BtsI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I
HindIII MmeI MscI MslI NaeI NgoMIV PflMI PfoI
PpuMI PstI SanDI SfcI SmlI StyI TaqII TatI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI BsaI BsaAI BsaHI BsaWI
BseRI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MspA1I NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI XbaI XcmI XhoI
XmaI ZraI