(Circular) (Six-base) MAPSORT of: pFN21AB8180.seq Check: 2525 from: 1 to: 5980 pFN21AB8180 5980 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3012 3323 1937 Size: 1075 311 4594 Fragments arranged by size: 4594 1075 311 Acc65I G'GTAC_C Cuts at: 3301 3301 Size: 5980 AclI AA'CG_TT Cuts at: 1563 4107 4480 1563 Size: 2544 373 3063 Fragments arranged by size: 3063 2544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2880 3182 4043 5427 5695 1632 Size: 1248 302 861 1384 268 1917 Fragments arranged by size: 1917 1384 1248 861 302 268 AfeI AGC'GCT Cuts at: 3058 3058 Size: 5980 AflII C'TTAA_G Cuts at: 820 1017 2963 820 Size: 197 1946 3837 Fragments arranged by size: 3837 1946 197 AflIII A'CryG_T Cuts at: 1203 4879 5856 1203 Size: 3676 977 1327 Fragments arranged by size: 3676 1327 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 4709 4709 Size: 5980 AleI CACnn'nnGTG Cuts at: 1166 3269 1166 Size: 2103 3877 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2582 2585 2892 1184 Size: 1398 3 307 4272 Fragments arranged by size: 4272 1398 307 3 AlwNI CAG_nnn'CTG Cuts at: 2818 3723 5295 2818 Size: 905 1572 3503 Fragments arranged by size: 3503 1572 905 ApaI G_GGCC'C Cuts at: 2488 2845 2488 Size: 357 5623 ApaLI G'TGCA_C Cuts at: 2877 4037 5193 2877 Size: 1160 1156 3664 Fragments arranged by size: 3664 1160 1156 ApoI r'AATT_y Cuts at: 1493 3095 3289 3515 3667 5776 1493 Size: 1602 194 226 152 2109 1697 Fragments arranged by size: 2109 1697 1602 226 194 152 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 2838 3305 1870 Size: 73 895 467 4545 Fragments arranged by size: 4545 895 467 73 BamHI G'GATC_C Cuts at: 3310 3677 3310 Size: 367 5613 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2533 2750 3301 4756 618 Size: 325 217 627 746 217 551 1455 1842 Fragments arranged by size: 1842 1455 746 627 551 325 217 217 BanII G_rGCy'C Cuts at: 1391 2013 2032 2488 2832 2845 3299 1391 Size: 622 19 456 344 13 454 4072 Fragments arranged by size: 4072 622 456 454 344 19 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2894 2914 928 Size: 971 995 20 3994 Fragments arranged by size: 3994 995 971 20 BbvCI CC'TCA_GC Cuts at: 2014 2758 2014 Size: 744 5236 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4171 4205 1903 Size: 34 2234 34 3678 Fragments arranged by size: 3678 2234 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3872 5082 3872 Size: 1210 4770 BclI T'GATC_A Cuts at: 1548 1548 Size: 5980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2165 2483 2855 Size: 107 122 71 631 605 493 318 372 Cuts at: 2855 3138 4591 136 Size: 283 1453 1525 Fragments arranged by size: 1525 1453 631 605 493 372 318 283 122 107 71 BglII A'GATC_T Cuts at: 2643 5975 2643 Size: 3332 2648 BlpI GC'TnA_GC Cuts at: 3397 3397 Size: 5980 Bme1580I G_kGCm'C Cuts at: 1790 2488 2538 2845 2881 4041 5197 1790 Size: 698 50 307 36 1160 1156 2573 Fragments arranged by size: 2573 1160 1156 698 307 50 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2696 3711 4669 Size: 416 53 83 399 24 1279 1015 958 Cuts at: 4669 442 Size: 1753 Fragments arranged by size: 1753 1279 1015 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2582 1056 Size: 1526 4454 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2061 2828 4640 1239 Size: 822 767 1812 2579 Fragments arranged by size: 2579 1812 822 767 Bpu10I CC'TnA_GC Cuts at: 2014 2574 2758 2014 Size: 560 184 5236 Fragments arranged by size: 5236 560 184 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4105 4970 5268 4105 Size: 865 298 4817 Fragments arranged by size: 4817 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4643 882 Size: 617 3144 2219 Fragments arranged by size: 3144 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3771 493 Size: 711 2567 2702 Fragments arranged by size: 2702 2567 711 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3787 4169 4804 275 Size: 53 83 186 936 2254 382 635 1451 Fragments arranged by size: 2254 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4413 4811 5085 5232 5643 4413 Size: 398 274 147 411 4750 Fragments arranged by size: 4750 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5755 Fragments arranged by size: 5755 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3172 1186 Size: 1986 3994 BseYI C'CCAG_C Cuts at: 2619 2797 2832 5183 5679 2619 Size: 178 35 2351 496 2920 Fragments arranged by size: 2920 2351 496 178 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5407 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3430 4191 5219 1540 Size: 275 186 1429 761 1028 2301 Fragments arranged by size: 2301 1429 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2474 2881 3255 4041 4126 5197 2474 Size: 407 374 786 85 1071 3257 Fragments arranged by size: 3257 1071 786 407 374 85 BsmI GAATG_Cn' Cuts at: 2561 3491 3584 2561 Size: 930 93 4957 Fragments arranged by size: 4957 930 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2032 2474 2488 2538 2832 2845 Size: 399 223 19 442 14 50 294 13 Cuts at: 2845 2881 3255 3299 4041 4126 5197 1391 Size: 36 374 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 442 399 374 294 223 85 50 44 36 19 14 13 BspEI T'CCGG_A Cuts at: 5643 5643 Size: 5980 BspHI T'CATG_A Cuts at: 3869 5599 3869 Size: 1730 4250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2946 3335 844 Size: 2102 389 3489 Fragments arranged by size: 3489 2102 389 BsrBI CCG'CTC Cuts at: 1394 1823 3180 3867 1394 Size: 429 1357 687 3507 Fragments arranged by size: 3507 1357 687 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4475 4649 65 Size: 1183 3227 174 1396 Fragments arranged by size: 3227 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2305 4624 4811 1700 Size: 110 495 2319 187 2869 Fragments arranged by size: 2869 2319 495 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5980 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 4040 5052 4040 Size: 1012 4968 BstAPI GCAn_nnn'nTGC Cuts at: 794 3250 794 Size: 2456 3524 BstBI TT'CG_AA Cuts at: 4784 4784 Size: 5980 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5980 BstXI CCAn_nnnn'nTGG Cuts at: 2317 2317 Size: 5980 BstYI r'GATC_y Cuts at: 1972 2643 2742 3310 3677 4062 4079 5531 5617 Size: 671 99 568 367 385 17 1452 86 Cuts at: 5617 5629 5975 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 671 568 385 367 346 99 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2488 2700 513 Size: 552 939 484 212 3793 Fragments arranged by size: 3793 939 552 484 212 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2137 2407 487 Size: 683 56 911 270 4060 Fragments arranged by size: 4060 911 683 270 56 BtsI GCAGTG_nn' Cuts at: 2409 2569 3496 4290 4310 2409 Size: 160 927 794 20 4079 Fragments arranged by size: 4079 927 794 160 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5945 DraI TTT'AAA Cuts at: 3284 3284 Size: 5980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4987 809 Size: 507 3671 1802 Fragments arranged by size: 3671 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2307 2347 Size: 54 1425 50 275 18 381 96 40 Cuts at: 2347 2523 2697 3427 4316 5945 8 Size: 176 174 730 889 1629 43 Fragments arranged by size: 1629 1425 889 730 381 275 176 174 96 54 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 3427 1537 Size: 275 1615 4090 Fragments arranged by size: 4090 1615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2603 3910 1353 Size: 1250 1307 3423 Fragments arranged by size: 3423 1307 1250 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2236 2975 4567 4941 5087 387 Size: 1064 138 647 739 1592 374 146 1280 Fragments arranged by size: 1592 1280 1064 739 647 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2828 2880 3182 4043 4640 5427 Size: 393 429 767 52 302 861 597 787 Cuts at: 5427 5695 1239 Size: 268 1524 Fragments arranged by size: 1524 861 787 767 597 429 393 302 268 52 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2810 2360 Size: 450 5530 EcoO109I rG'GnC_Cy Cuts at: 2041 2146 2803 2815 3146 3166 2041 Size: 105 657 12 331 20 4855 Fragments arranged by size: 4855 657 331 105 20 12 EcoRI G'AATT_C Cuts at: 3289 3667 3289 Size: 378 5602 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3136 3136 Size: 5980 FspI TGC'GCA Cuts at: 4486 4486 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 2785 3060 5127 1678 Size: 1107 275 2067 2531 Fragments arranged by size: 2531 2067 1107 275 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3210 3242 4621 4653 4695 Size: 32 135 32 1638 32 1379 32 42 Cuts at: 4695 4727 1373 Size: 32 2626 Fragments arranged by size: 2626 1638 1379 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2263 3324 3570 669 Size: 988 281 325 1061 246 3079 Fragments arranged by size: 3079 1061 988 325 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5980 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KpnI G_GTAC'C Cuts at: 3305 3305 Size: 5980 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5094 5278 1305 Size: 3789 184 2007 Fragments arranged by size: 3789 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2525 2699 10 Size: 54 1425 343 693 174 3291 Fragments arranged by size: 3291 1425 693 343 174 54 MslI CAynn'nnrTG Cuts at: 518 1166 2531 2699 3269 3938 4297 4456 518 Size: 648 1365 168 570 669 359 159 2042 Fragments arranged by size: 2042 1365 669 648 570 359 168 159 MspA1I CmG'CkG Cuts at: 1606 2436 2797 3396 4073 5221 5466 1606 Size: 830 361 599 677 1148 245 2120 Fragments arranged by size: 2120 1148 830 677 599 361 245 NaeI GCC'GGC Cuts at: 2307 2307 Size: 5980 NcoI C'CATG_G Cuts at: 513 1065 2004 2700 513 Size: 552 939 696 3793 Fragments arranged by size: 3793 939 696 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NgoMIV G'CCGG_C Cuts at: 2305 2305 Size: 5980 NheI G'CTAG_C Cuts at: 1052 2578 1052 Size: 1526 4454 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 3248 3338 4883 1132 Size: 97 2019 90 1545 2229 Fragments arranged by size: 2229 2019 1545 97 90 PasI CC'CwG_GG Cuts at: 1348 2024 1348 Size: 676 5304 PciI A'CATG_T Cuts at: 4879 4879 Size: 5980 PflMI CCAn_nnn'nTGG Cuts at: 1108 3040 1108 Size: 1932 4048 PfoI T'CCnGG_A Cuts at: 3099 3099 Size: 5980 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2041 2146 2803 3146 2041 Size: 105 657 343 4875 Fragments arranged by size: 4875 657 343 105 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5980 PsiI TTA'TAA Cuts at: 3550 3550 Size: 5980 PspOMI G'GGCC_C Cuts at: 2484 2841 2484 Size: 357 5623 PstI C_TGCA'G Cuts at: 830 2548 3332 830 Size: 1718 784 3478 Fragments arranged by size: 3478 1718 784 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5980 PvuII CAG'CTG Cuts at: 2797 2797 Size: 5980 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SanDI GG'GwC_CC Cuts at: 2041 3146 2041 Size: 1105 4875 SapI GCTCTTCn'nnn_ Cuts at: 2603 2603 Size: 5980 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SfcI C'TryA_G Cuts at: 826 1045 2378 2544 3328 4463 5144 5335 826 Size: 219 1333 166 784 1135 681 191 1471 Fragments arranged by size: 1471 1333 1135 784 681 219 191 166 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 2840 3307 1872 Size: 968 467 4545 Fragments arranged by size: 4545 968 467 SmlI C'TyrA_G Cuts at: 820 1017 1943 2963 4084 4985 5247 820 Size: 197 926 1020 1121 901 262 1553 Fragments arranged by size: 1553 1121 1020 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3338 1132 Size: 2206 3774 SspI AAT'ATT Cuts at: 5 52 3904 5 Size: 47 3852 2081 Fragments arranged by size: 3852 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5980 StyI C'CwwG_G Cuts at: 513 1065 2004 2700 3419 513 Size: 552 939 696 719 3074 Fragments arranged by size: 3074 939 719 696 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2469 4007 4024 4177 4362 4807 325 Size: 1255 889 1538 17 153 185 445 1498 Fragments arranged by size: 1538 1498 1255 889 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4226 5938 96 Size: 274 80 33 51 1443 2249 1712 138 Fragments arranged by size: 2249 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5940 5972 5940 Size: 32 5948 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5980 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2317 2761 2902 2317 Size: 444 141 5395 Fragments arranged by size: 5395 444 141 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5980 XmaI C'CCGG_G Cuts at: 1870 2838 3305 1870 Size: 968 467 4545 Fragments arranged by size: 4545 968 467 XmnI GAAnn'nnTTC Cuts at: 4109 4109 Size: 5980 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI