Restriction map for
pFN21AB8176
(Circular) (Six-base) MAPSORT of: pFN21AB8176.seq Check: 6766 from: 1 to: 5440
pFN21AB8176 5440 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2084 2084
Size: 5440
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3250 278
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2783 1937
Size: 846 4594
Acc65I G'GTAC_C
Cuts at: 2385 2761 2385
Size: 376 5064
AclI AA'CG_TT
Cuts at: 1563 2739 3567 3940 1563
Size: 1176 828 373 3063
Fragments arranged by size:
3063 1176 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2415 3503 4887 5155 1632
Size: 783 1088 1384 268 1917
Fragments arranged by size:
1917 1384 1088 783 268
AfeI AGC'GCT
Cuts at: 2057 2057
Size: 5440
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5243
AflIII A'CryG_T
Cuts at: 1203 4339 5316 1203
Size: 3136 977 1327
Fragments arranged by size:
3136 1327 977
AgeI A'CCGG_T
Cuts at: 4271 4271
Size: 5440
AhdI GACnn_n'nnGTC
Cuts at: 4169 4169
Size: 5440
AleI CACnn'nnGTG
Cuts at: 1166 2291 1166
Size: 1125 4315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2309 1184
Size: 1125 4315
AlwNI CAG_nnn'CTG
Cuts at: 3183 4755 3183
Size: 1572 3868
ApaLI G'TGCA_C
Cuts at: 3497 4653 3497
Size: 1156 4284
ApoI r'AATT_y
Cuts at: 1493 2749 2975 3127 5236 1493
Size: 1256 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3994 160
Size: 872 2962 1606
Fragments arranged by size:
2962 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
AvaI C'yCGr_G
Cuts at: 1870 1943 2765 1870
Size: 73 822 4545
Fragments arranged by size:
4545 822 73
BamHI G'GATC_C
Cuts at: 2770 3137 2770
Size: 367 5073
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2385 2761 4216 618
Size: 325 217 627 315 283 376 1455 1842
Fragments arranged by size:
1842 1455 627 376 325 315 283 217
BanII G_rGCy'C
Cuts at: 1391 2759 1391
Size: 1368 4072
BbeI G_GCGC'C
Cuts at: 2106 2106
Size: 5440
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2018 2213 2344 2634 928
Size: 971 119 195 131 290 3734
Fragments arranged by size:
3734 971 290 195 131 119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3631 3665 1903
Size: 34 1694 34 3678
Fragments arranged by size:
3678 1694 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3332 4542 3332
Size: 1210 4230
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5440
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 2048 4051
Size: 107 122 71 631 605 367 9 2003
Cuts at: 4051 136
Size: 1525
Fragments arranged by size:
2003 1525 631 605 367 122 107 71
9
BglII A'GATC_T
Cuts at: 5435 5435
Size: 5440
BlpI GC'TnA_GC
Cuts at: 2857 2857
Size: 5440
Bme1580I G_kGCm'C
Cuts at: 1790 3501 4657 1790
Size: 1711 1156 2573
Fragments arranged by size:
2573 1711 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5440
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3171 4129 442
Size: 416 53 83 399 24 1754 958 1753
Fragments arranged by size:
1754 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2200 4100 1239
Size: 961 1900 2579
Fragments arranged by size:
2579 1900 961
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3565 4430 4728 3565
Size: 865 298 4277
Fragments arranged by size:
4277 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4103 882
Size: 617 2604 2219
Fragments arranged by size:
2604 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3231 493
Size: 711 2027 2702
Fragments arranged by size:
2702 2027 711
BsaBI GATnn'nnATC
Cuts at: 5434 5434
Size: 5440
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 3247 3629 4264
Size: 53 83 186 936 570 1144 382 635
Cuts at: 4264 275
Size: 1451
Fragments arranged by size:
1451 1144 936 635 570 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2382 3873 4271 4545 4692 5103 2382
Size: 1491 398 274 147 411 2719
Fragments arranged by size:
2719 1491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2150 1178
Size: 30 165 30 717 30 4468
Fragments arranged by size:
4468 717 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 1186
Size: 949 4491
BseYI C'CCAG_C
Cuts at: 4643 5139 4643
Size: 496 4944
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2269 2554 2721 1163
Size: 573 533 285 167 3882
Fragments arranged by size:
3882 573 533 285 167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2890 3651 4679 1540
Size: 275 186 889 761 1028 2301
Fragments arranged by size:
2301 1028 889 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3501 3586 4657 3501
Size: 85 1071 4284
Fragments arranged by size:
4284 1071 85
BsmI GAATG_Cn'
Cuts at: 2951 3044 2951
Size: 93 5347
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2759 3501 3586 4657 1391
Size: 399 969 742 85 1071 2174
Fragments arranged by size:
2174 1071 969 742 399 85
BspEI T'CCGG_A
Cuts at: 5103 5103
Size: 5440
BspHI T'CATG_A
Cuts at: 3329 5059 3329
Size: 1730 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2084 2139 2160 2795 844
Size: 1240 55 21 635 3489
Fragments arranged by size:
3489 1240 635 55 21
BsrBI CCG'CTC
Cuts at: 1394 1823 3327 1394
Size: 429 1504 3507
Fragments arranged by size:
3507 1504 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2601 3935 4109 65
Size: 1183 1353 1334 174 1396
Fragments arranged by size:
1396 1353 1334 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4084 4271 1700
Size: 110 2274 187 2869
Fragments arranged by size:
2869 2274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5440
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5440
BssSI C'ACGA_G
Cuts at: 3500 4512 3500
Size: 1012 4428
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5440
BstBI TT'CG_AA
Cuts at: 4244 4244
Size: 5440
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5440
BstXI CCAn_nnnn'nTGG
Cuts at: 2296 2296
Size: 5440
BstYI r'GATC_y
Cuts at: 1972 2276 2770 3137 3522 3539 4991 5077 5089
Size: 304 494 367 385 17 1452 86 12
Cuts at: 5089 5435 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 494 385 367 346 304 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2139 2289 513
Size: 552 939 135 150 3664
Fragments arranged by size:
3664 939 552 150 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4701
Fragments arranged by size:
4701 683 56
BtsI GCAGTG_nn'
Cuts at: 2094 2956 3750 3770 2094
Size: 862 794 20 3764
Fragments arranged by size:
3764 862 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5405
DraI TTT'AAA
Cuts at: 2744 2744
Size: 5440
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4447 809
Size: 507 3131 1802
Fragments arranged by size:
3131 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2070 2887
Size: 54 1425 50 275 18 210 30 817
Cuts at: 2887 3776 5405 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 817 275 210 54 50
43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2887 1537
Size: 275 1075 4090
Fragments arranged by size:
4090 1075 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3370 1353
Size: 2017 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2164 2492 4027 4401 4547 387
Size: 1064 138 575 328 1535 374 146 1280
Fragments arranged by size:
1535 1280 1064 575 374 328 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2200 2415 3503 4100 4887 5155 1239
Size: 393 568 215 1088 597 787 268 1524
Fragments arranged by size:
1524 1088 787 597 568 393 268 215
EcoRI G'AATT_C
Cuts at: 2749 3127 2749
Size: 378 5062
FspI TGC'GCA
Cuts at: 3946 3946
Size: 5440
HaeII r_GCGC'y
Cuts at: 1678 2059 2106 4587 1678
Size: 381 47 2481 2531
Fragments arranged by size:
2531 2481 381 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 4081 4113 4155
Size: 32 135 32 689 32 1788 32 42
Cuts at: 4155 4187 1373
Size: 32 2626
Fragments arranged by size:
2626 1788 689 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2784 3030 669
Size: 988 281 846 246 3079
Fragments arranged by size:
3079 988 846 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5440
HpaI GTT'AAC
Cuts at: 3030 3030
Size: 5440
KasI G'GCGC_C
Cuts at: 2102 2102
Size: 5440
KpnI G_GTAC'C
Cuts at: 2389 2765 2389
Size: 376 5064
MfeI C'AATT_G
Cuts at: 3039 3039
Size: 5440
MluI A'CGCG_T
Cuts at: 5316 5316
Size: 5440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 2244 2286 4554 4738 1305
Size: 834 105 42 2268 184 2007
Fragments arranged by size:
2268 2007 834 184 105 42
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2072 10
Size: 54 1425 343 240 3378
Fragments arranged by size:
3378 1425 343 240 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 2291 3398 3757 3916 518
Size: 648 912 213 1107 359 159 2042
Fragments arranged by size:
2042 1107 912 648 359 213 159
MspA1I CmG'CkG
Cuts at: 1606 2236 2856 3533 4681 4926 1606
Size: 630 620 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 630 620 245
NarI GG'CG_CC
Cuts at: 2103 2103
Size: 5440
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3949
Fragments arranged by size:
3949 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5440
NotI GC'GGCC_GC
Cuts at: 2887 2887
Size: 5440
NspI r_CATG'y
Cuts at: 1132 1229 2798 4343 1132
Size: 97 1569 1545 2229
Fragments arranged by size:
2229 1569 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5440
PciI A'CATG_T
Cuts at: 4339 4339
Size: 5440
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5440
PmeI GTTT'AAAC
Cuts at: 2744 2744
Size: 5440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 5022 5054 3509
Size: 32 1481 32 3895
Fragments arranged by size:
3895 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5440
PsiI TTA'TAA
Cuts at: 3010 3010
Size: 5440
PstI C_TGCA'G
Cuts at: 830 2792 830
Size: 1962 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5440
PvuII CAG'CTG
Cuts at: 2236 2236
Size: 5440
SalI G'TCGA_C
Cuts at: 1936 2782 1936
Size: 846 4594
SbfI CC_TGCA'GG
Cuts at: 2792 2792
Size: 5440
ScaI AGT'ACT
Cuts at: 3688 5400 3688
Size: 1712 3728
SfcI C'TryA_G
Cuts at: 826 1045 2788 3923 4604 4795 826
Size: 219 1743 1135 681 191 1471
Fragments arranged by size:
1743 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2039 2048 2039
Size: 9 5431
SfoI GGC'GCC
Cuts at: 2104 2104
Size: 5440
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5440
SmaI CCC'GGG
Cuts at: 1872 2767 1872
Size: 895 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3544 4445 4707 820
Size: 197 926 1601 901 262 1553
Fragments arranged by size:
1601 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3231 493
Size: 2738 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5440
SphI G_CATG'C
Cuts at: 1132 2798 1132
Size: 1666 3774
SspI AAT'ATT
Cuts at: 5 52 2315 3364 5
Size: 47 2263 1049 2081
Fragments arranged by size:
2263 2081 1049 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5440
StyI C'CwwG_G
Cuts at: 513 1065 2004 2440 2879 513
Size: 552 939 436 439 3074
Fragments arranged by size:
3074 939 552 439 436
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2108 2225 2511 3467 3484 3637 3822
Size: 1255 528 117 286 956 17 153 185
Cuts at: 3822 4267 325
Size: 445 1498
Fragments arranged by size:
1498 1255 956 528 445 286 185 153
117 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3686 5398 96
Size: 274 80 33 51 1443 1709 1712 138
Fragments arranged by size:
1712 1709 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2484 2516 5400 5432 2484
Size: 32 2884 32 2492
Fragments arranged by size:
2884 2492 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5440
XbaI T'CTAG_A
Cuts at: 2776 2776
Size: 5440
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5440
XmaI C'CCGG_G
Cuts at: 1870 2765 1870
Size: 895 4545
XmnI GAAnn'nnTTC
Cuts at: 3569 3569
Size: 5440
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3248 276
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SrfI SwaI
XcmI