Restriction map for
pFN21AB8176
(Linear) (Six-base) MAPSORT of: KIBB8176.seq Check: 7344 from: 1 to: 735
KIBB8176.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 79 735
Size: 79 656
Acc65I G'GTAC_C
Cuts at: 0 380 735
Size: 380 355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 735
Size: 410 325
AfeI AGC'GCT
Cuts at: 0 52 735
Size: 52 683
AleI CACnn'nnGTG
Cuts at: 0 286 735
Size: 286 449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 304 735
Size: 304 431
BanI G'GyrC_C
Cuts at: 0 97 380 735
Size: 97 283 355
Fragments arranged by size:
355 283 97
BbeI G_GCGC'C
Cuts at: 0 101 735
Size: 101 634
BbsI GAAGACnn'nnnn_
Cuts at: 0 13 208 339 629 735
Size: 13 195 131 290 106
Fragments arranged by size:
290 195 131 106 13
BglI GCCn_nnn'nGGC
Cuts at: 0 34 43 735
Size: 34 9 692
Fragments arranged by size:
692 34 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 735
Size: 195 540
BsaHI Gr'CG_yC
Cuts at: 0 98 735
Size: 98 637
BsaWI w'CCGG_w
Cuts at: 0 377 735
Size: 377 358
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 735
Size: 115 30 590
Fragments arranged by size:
590 115 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 735
Size: 130 605
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 549 716 735
Size: 264 285 167 19
Fragments arranged by size:
285 264 167 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 79 134 155 735
Size: 79 55 21 580
Fragments arranged by size:
580 79 55 21
BsrDI GCAATG_nn'
Cuts at: 0 596 735
Size: 596 139
BstXI CCAn_nnnn'nTGG
Cuts at: 0 291 735
Size: 291 444
BstYI r'GATC_y
Cuts at: 0 271 735
Size: 271 464
BtgI C'CryG_G
Cuts at: 0 134 284 735
Size: 134 150 451
Fragments arranged by size:
451 150 134
BtsI GCAGTG_nn'
Cuts at: 0 89 735
Size: 89 646
EaeI y'GGCC_r
Cuts at: 0 35 65 735
Size: 35 30 670
Fragments arranged by size:
670 35 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 159 487 735
Size: 159 328 248
Fragments arranged by size:
328 248 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 410 735
Size: 195 215 325
Fragments arranged by size:
325 215 195
HaeII r_GCGC'y
Cuts at: 0 54 101 735
Size: 54 47 634
Fragments arranged by size:
634 54 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 735
Size: 256 32 447
Fragments arranged by size:
447 256 32
KasI G'GCGC_C
Cuts at: 0 97 735
Size: 97 638
KpnI G_GTAC'C
Cuts at: 0 384 735
Size: 384 351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 239 281 735
Size: 134 105 42 454
Fragments arranged by size:
454 134 105 42
MscI TGG'CCA
Cuts at: 0 67 735
Size: 67 668
MslI CAynn'nnrTG
Cuts at: 0 73 286 735
Size: 73 213 449
Fragments arranged by size:
449 213 73
MspA1I CmG'CkG
Cuts at: 0 231 735
Size: 231 504
NarI GG'CG_CC
Cuts at: 0 98 735
Size: 98 637
PvuII CAG'CTG
Cuts at: 0 231 735
Size: 231 504
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 34 43 735
Size: 34 9 692
Fragments arranged by size:
692 34 9
SfoI GGC'GCC
Cuts at: 0 99 735
Size: 99 636
SspI AAT'ATT
Cuts at: 0 310 735
Size: 310 425
StyI C'CwwG_G
Cuts at: 0 435 735
Size: 435 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 103 220 506 735
Size: 103 117 286 229
Fragments arranged by size:
286 229 117 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 479 511 735
Size: 479 32 224
Fragments arranged by size:
479 224 32
Enzymes that do cut:
AarI Acc65I AcuI AfeI AleI AlfI BanI BbeI
BbsI BglI BpmI BsaHI BsaWI BsaXI BseRI BsgI
BspMI BsrDI BstXI BstYI BtgI BtsI EaeI EciI
Eco57MI HaeII Hin4I KasI KpnI MmeI MscI MslI
MspA1I NarI PvuII SfiI SfoI SspI StyI TaqII
TstI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AloI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI MfeI MluI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI