Restriction map for
pFN21AB8141
##################################
# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AflIII AhdI Alw44I AlwI
AlwNI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BclI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseRI BsgI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstYI BstZ17I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MflI MluI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspI
PacI PaeR7I PflFI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SapI SbfI ScaI SfaNI SfiI
SfoI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tsp509I TspRI Tth111I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 171 GTCGAC GT^[AC][GT]AC
AccII 225 CGCG CG^CG
AccIII 188 TCCGGA T^CCGGA
AfaI 297 GTAC GT^AC
AgeI 34 ACCGGT A^CCGGT
AocI 246 CCTGAGG CC^T[ACGT]AGG
Aor51HI 85 AGCGCT AGC^GCT
AvaI 130 CCCGGG C^[CT]CG[GA]G
AvaII 134 GGACC G^G[AT]CC
BalI 62 TGGCCA TGG^CCA
BciVI 389 GGATAC GTATCC(6/5)
BfaI 287 CTAG C^TAG
BseAI 188 TCCGGA T^CCGGA
BsiEI 173 CGACCG CG[GA][CT]^CG
BsmFI 133 GGGAC GGGAC(10/14)
BsoBI 130 CCCGGG C^[CT]CG[GA]G
BspEI 188 TCCGGA T^CCGGA
BsrI 375 CCAGT ACTGG(1/-1)
BstBI 313 TTCGAA TT^CGAA
BstUI 225 CGCG CG^CG
Bsu36I 246 CCTGAGG CC^T[ACGT]AGG
DpnII 222 GATC ^GATC
DsaI 137 CCGTGG C^C[GA][CT]GG
EaeI 62 TGGCCA [CT]^GGCC[GA]
Eco47III 85 AGCGCT AGC^GCT
Eco81I 246 CCTGAGG CC^T[ACGT]AGG
EcoNI 200 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 171 GTCGAC GT[CT]^[GA]AC
HindII 171 GTCGAC GT[CT]^[GA]AC
MaeI 287 CTAG C^TAG
MaeIII 352 GTCAC ^GT[ACGT]AC
MboI 222 GATC ^GATC
MluNI 62 TGGCCA TGG^CCA
MroI 188 TCCGGA T^CCGGA
MscI 62 TGGCCA TGG^CCA
MslI 354 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 58 CAGCTG C[AC]G^C[GT]G
MvnI 225 CGCG CG^CG
NdeII 222 GATC ^GATC
NheI 286 GCTAGC G^CTAGC
NlaIII 360 CATG CATG^
NlaIV 133 GGGACC GG[ACGT]^[ACGT]CC
NspV 313 TTCGAA TT^CGAA
PflMI 204 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 34 ACCGGT A^CCGGT
PleI 350 GAGTC GAGTC(4/5)
PstI 277 CTGCAG CTGCA^G
PvuII 58 CAGCTG CAG^CTG
RsaI 297 GTAC GT^AC
SalI 171 GTCGAC G^TCGAC
Sau3AI 222 GATC ^GATC
SexAI 299 ACCTGGT A^CC[AT]GGT
SfuI 313 TTCGAA TT^CGAA
SgrAI 33 CACCGGTG C[GA]^CCGG[CT]G
SmaI 130 CCCGGG CCC^GGG
TfiI 153 GATTC G^A[AT]TC
Tsp45I 352 GTCAC ^GT[GC]AC
Van91I 204 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 130 CCCGGG C^CCGGG
XmaI 130 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BcgI 173 CGACCGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
15 GCAGACTGCTCG
BcnI 130 CCCGG CC^[GC]GG
131 CCGGG
BsaJI 130 CCCGGG C^C[ACGT][ACGT]GG
137 CCGTGG
BsaWI 34 ACCGGT [AT]^CCGG[AT]
188 TCCGGA
BsrFI 34 ACCGGT [GA]^CCGG[CT]
97 ACCGGC
BstNI 77 CCAGG CC^[AT]GG
300 CCTGG
Cfr10I 34 ACCGGT [GA]^CCGG[CT]
97 ACCGGC
DdeI 247 CTGAG C^T[ACGT]AG
344 CTAAG
EcoRII 77 CCAGG ^CC[AT]GG
300 CCTGG
FauI 50 CCCGC CCCGC(4/6)
101 GCGGG
HaeII 85 AGCGCT [GA]GCGC^[CT]
141 GGCGCT
HinfI 153 GATTC G^A[ACGT]TC
350 GAGTC
MboII 108 GAAGA GAAGA(8/7)
316 GAAGA
MvaI 77 CCAGG CC^[AT]GG
300 CCTGG
NciI 130 CCCGG CC^[GC]GG
131 CCGGG
SfcI 123 CTACAG C^T[GA][CT]AG
277 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
BssKI BstF5I Cac8I CfoI Cfr13I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MnlI
MspI MwoI Sau96I ScrFI TaqI
TseI TthHB8I