(Circular) (Six-base) MAPSORT of: pFN21AB8141.seq Check: 5970 from: 1 to: 5113 pFN21AB8141 5113 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2923 278 Size: 53 83 186 936 1387 2468 Fragments arranged by size: 2468 1387 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2177 2456 1937 Size: 240 279 4594 Fragments arranged by size: 4594 279 240 Acc65I G'GTAC_C Cuts at: 2434 2434 Size: 5113 AclI AA'CG_TT Cuts at: 1563 3240 3613 1563 Size: 1677 373 3063 Fragments arranged by size: 3063 1677 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3176 4560 4828 1632 Size: 1544 1384 268 1917 Fragments arranged by size: 1917 1544 1384 268 AfeI AGC'GCT Cuts at: 2092 2092 Size: 5113 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4916 AflIII A'CryG_T Cuts at: 1203 4012 4989 1203 Size: 2809 977 1327 Fragments arranged by size: 2809 1327 977 AgeI A'CCGG_T Cuts at: 2039 3944 2039 Size: 1905 3208 AhdI GACnn_n'nnGTC Cuts at: 3842 3842 Size: 5113 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5113 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5113 AlwNI CAG_nnn'CTG Cuts at: 2856 4428 2856 Size: 1572 3541 ApaLI G'TGCA_C Cuts at: 3170 4326 3170 Size: 1156 3957 ApoI r'AATT_y Cuts at: 1493 2422 2648 2800 4909 1493 Size: 929 226 152 2109 1697 Fragments arranged by size: 2109 1697 929 226 152 AseI AT'TA_AT Cuts at: 160 1032 3667 160 Size: 872 2635 1606 Fragments arranged by size: 2635 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5113 AvaI C'yCGr_G Cuts at: 1870 1943 2135 2438 1870 Size: 73 192 303 4545 Fragments arranged by size: 4545 303 192 73 BamHI G'GATC_C Cuts at: 2443 2810 2443 Size: 367 4746 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2434 3889 618 Size: 325 217 627 647 1455 1842 Fragments arranged by size: 1842 1455 647 627 325 217 BanII G_rGCy'C Cuts at: 1391 2432 1391 Size: 1041 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2009 2043 2167 2201 3304 3338 1903 Size: 34 72 34 124 34 1103 34 3678 Fragments arranged by size: 3678 1103 124 72 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2388 3005 4215 2388 Size: 617 1210 3286 Fragments arranged by size: 3286 1210 617 BclI T'GATC_A Cuts at: 1548 1548 Size: 5113 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3724 136 Size: 107 122 71 631 605 2052 1525 Fragments arranged by size: 2052 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5108 5108 Size: 5113 BlpI GC'TnA_GC Cuts at: 2530 2530 Size: 5113 Bme1580I G_kGCm'C Cuts at: 1790 3174 4330 1790 Size: 1384 1156 2573 Fragments arranged by size: 2573 1384 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5113 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2844 3802 442 Size: 416 53 83 399 24 1427 958 1753 Fragments arranged by size: 1753 1427 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2295 1056 Size: 1239 3874 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3773 1239 Size: 2534 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3238 4103 4401 3238 Size: 865 298 3950 Fragments arranged by size: 3950 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3776 882 Size: 617 2277 2219 Fragments arranged by size: 2277 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2904 493 Size: 711 1700 2702 Fragments arranged by size: 2702 1700 711 BsaBI GATnn'nnATC Cuts at: 5107 5107 Size: 5113 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2920 3302 3937 275 Size: 53 83 186 936 1387 382 635 1451 Fragments arranged by size: 1451 1387 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2039 2193 3546 3944 4218 4365 4776 2039 Size: 154 1353 398 274 147 411 2376 Fragments arranged by size: 2376 1353 411 398 274 154 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4888 Fragments arranged by size: 4888 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5113 BseYI C'CCAG_C Cuts at: 4316 4812 4316 Size: 496 4617 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4540 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2181 2563 3324 4352 1540 Size: 275 186 180 382 761 1028 2301 Fragments arranged by size: 2301 1028 761 382 275 186 180 BsiHKAI G_wGCw'C Cuts at: 3174 3259 4330 3174 Size: 85 1071 3957 Fragments arranged by size: 3957 1071 85 BsmI GAATG_Cn' Cuts at: 2624 2717 2624 Size: 93 5020 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2432 3174 3259 4330 1391 Size: 399 642 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 642 399 85 BspEI T'CCGG_A Cuts at: 2193 4776 2193 Size: 2583 2530 BspHI T'CATG_A Cuts at: 3002 4732 3002 Size: 1730 3383 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2468 844 Size: 1624 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3000 1394 Size: 429 1177 3507 Fragments arranged by size: 3507 1177 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3608 3782 65 Size: 1183 2360 174 1396 Fragments arranged by size: 2360 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2039 2102 3757 3944 1700 Size: 110 229 63 1655 187 2869 Fragments arranged by size: 2869 1655 229 187 110 63 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5113 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5113 BssSI C'ACGA_G Cuts at: 3173 4185 3173 Size: 1012 4101 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5113 BstBI TT'CG_AA Cuts at: 2319 3917 2319 Size: 1598 3515 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5113 BstYI r'GATC_y Cuts at: 1972 2443 2810 3195 3212 4664 4750 4762 5108 Size: 471 367 385 17 1452 86 12 346 Cuts at: 5108 1972 Size: 1977 Fragments arranged by size: 1977 1452 471 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2252 2252 Size: 5113 BtgI C'CryG_G Cuts at: 513 1065 2004 2142 513 Size: 552 939 138 3484 Fragments arranged by size: 3484 939 552 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2108 487 Size: 683 56 882 3492 Fragments arranged by size: 3492 882 683 56 BtsI GCAGTG_nn' Cuts at: 2629 3423 3443 2629 Size: 794 20 4299 Fragments arranged by size: 4299 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5113 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5078 DraI TTT'AAA Cuts at: 2417 2417 Size: 5113 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4120 809 Size: 507 2804 1802 Fragments arranged by size: 2804 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2560 3449 Size: 54 1425 50 275 18 237 493 889 Cuts at: 3449 5078 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 493 275 237 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2560 1537 Size: 275 748 4090 Fragments arranged by size: 4090 748 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3043 1353 Size: 1690 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3700 4074 4220 387 Size: 1064 138 2111 374 146 1280 Fragments arranged by size: 2111 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3176 3773 4560 4828 1239 Size: 393 1544 597 787 268 1524 Fragments arranged by size: 1544 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2209 2209 Size: 5113 EcoRI G'AATT_C Cuts at: 2422 2800 2422 Size: 378 4735 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2221 2221 Size: 5113 FspI TGC'GCA Cuts at: 3619 3619 Size: 5113 HaeII r_GCGC'y Cuts at: 1678 2094 2150 4260 1678 Size: 416 56 2110 2531 Fragments arranged by size: 2531 2110 416 56 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2346 2378 3754 3786 3828 Size: 32 135 32 774 32 1376 32 42 Cuts at: 3828 3860 1373 Size: 32 2626 Fragments arranged by size: 2626 1376 774 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2178 2457 2703 669 Size: 988 281 240 279 246 3079 Fragments arranged by size: 3079 988 281 279 246 240 HindIII A'AGCT_T Cuts at: 748 748 Size: 5113 HpaI GTT'AAC Cuts at: 2703 2703 Size: 5113 KpnI G_GTAC'C Cuts at: 2438 2438 Size: 5113 MfeI C'AATT_G Cuts at: 2712 2712 Size: 5113 MluI A'CGCG_T Cuts at: 4989 4989 Size: 5113 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4227 4411 1305 Size: 2922 184 2007 Fragments arranged by size: 2922 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2069 10 Size: 54 1425 343 237 3054 Fragments arranged by size: 3054 1425 343 237 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 3071 3430 3589 518 Size: 648 1197 708 359 159 2042 Fragments arranged by size: 2042 1197 708 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2065 2529 3206 4354 4599 1606 Size: 459 464 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 464 459 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3622 Fragments arranged by size: 3622 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5113 NheI G'CTAG_C Cuts at: 1052 2291 1052 Size: 1239 3874 NotI GC'GGCC_GC Cuts at: 2560 2560 Size: 5113 NspI r_CATG'y Cuts at: 1132 1229 2471 4016 1132 Size: 97 1242 1545 2229 Fragments arranged by size: 2229 1545 1242 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5113 PciI A'CATG_T Cuts at: 4012 4012 Size: 5113 PflMI CCAn_nnn'nTGG Cuts at: 1108 2215 1108 Size: 1107 4006 PmeI GTTT'AAAC Cuts at: 2417 2417 Size: 5113 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3182 3214 4695 4727 3182 Size: 32 1481 32 3568 Fragments arranged by size: 3568 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5113 PsiI TTA'TAA Cuts at: 2683 2683 Size: 5113 PstI C_TGCA'G Cuts at: 830 2286 2465 830 Size: 1456 179 3478 Fragments arranged by size: 3478 1456 179 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5113 PvuII CAG'CTG Cuts at: 2065 2065 Size: 5113 SalI G'TCGA_C Cuts at: 1936 2176 2455 1936 Size: 240 279 4594 Fragments arranged by size: 4594 279 240 SbfI CC_TGCA'GG Cuts at: 2465 2465 Size: 5113 ScaI AGT'ACT Cuts at: 3361 5073 3361 Size: 1712 3401 SexAI A'CCwGG_T Cuts at: 2304 2304 Size: 5113 SfcI C'TryA_G Cuts at: 826 1045 2128 2282 2461 3596 4277 4468 826 Size: 219 1083 154 179 1135 681 191 1471 Fragments arranged by size: 1471 1135 1083 681 219 191 179 154 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5113 SgrAI Cr'CCGG_yG Cuts at: 1700 2039 1700 Size: 339 4774 SmaI CCC'GGG Cuts at: 1872 2137 2440 1872 Size: 265 303 4545 Fragments arranged by size: 4545 303 265 SmlI C'TyrA_G Cuts at: 820 1017 1943 3217 4118 4380 820 Size: 197 926 1274 901 262 1553 Fragments arranged by size: 1553 1274 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2904 493 Size: 2411 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5113 SphI G_CATG'C Cuts at: 1132 2471 1132 Size: 1339 3774 SspI AAT'ATT Cuts at: 5 52 3037 5 Size: 47 2985 2081 Fragments arranged by size: 2985 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5113 StyI C'CwwG_G Cuts at: 513 1065 2004 2552 513 Size: 552 939 548 3074 Fragments arranged by size: 3074 939 552 548 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2195 3140 3157 3310 3495 3940 325 Size: 1255 615 945 17 153 185 445 1498 Fragments arranged by size: 1498 1255 945 615 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3359 5071 96 Size: 274 80 33 51 1443 1382 1712 138 Fragments arranged by size: 1712 1443 1382 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5073 5105 5073 Size: 32 5081 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5113 XbaI T'CTAG_A Cuts at: 2449 2449 Size: 5113 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5113 XmaI C'CCGG_G Cuts at: 1870 2135 2438 1870 Size: 265 303 4545 Fragments arranged by size: 4545 303 265 XmnI GAAnn'nnTTC Cuts at: 3242 3242 Size: 5113 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2921 276 Size: 53 83 186 936 1387 2468 Fragments arranged by size: 2468 1387 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI