Restriction map for pFN21AB8113
 (Linear) (Six-base) MAPSORT of: KIBB8113.seq  Check: 3868  from: 1  to: 1140



KIBB8113.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     279     787    1140 
   Size:        279     508     353

  Fragments arranged by size: 

                 508     353     279
 
AccI GT'mk_AC

Cuts at:      0      95    1140 
   Size:         95    1045
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     567    1140 
   Size:        567     573
 
AflIII A'CryG_T

Cuts at:      0      90    1140 
   Size:         90    1050
 
AleI CACnn'nnGTG

Cuts at:      0     865    1140 
   Size:        865     275
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     614     646    1140 
   Size:        614      32     494

  Fragments arranged by size: 

                 614     494      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     942    1140 
   Size:        942     198
 
BamHI G'GATC_C

Cuts at:      0     848    1140 
   Size:        848     292
 
BanI G'GyrC_C

Cuts at:      0     231    1140 
   Size:        231     909
 
BanII G_rGCy'C

Cuts at:      0     326     884    1045    1140 
   Size:        326     558     161      95

  Fragments arranged by size: 

                 558     326     161      95
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      83     792    1140 
   Size:         83     709     348

  Fragments arranged by size: 

                 709     348      83
 
BglI GCCn_nnn'nGGC

Cuts at:      0     451    1140 
   Size:        451     689
 
BglII A'GATC_T

Cuts at:      0     111    1140 
   Size:        111    1029
 
Bme1580I G_kGCm'C

Cuts at:      0     234    1140 
   Size:        234     906
 
BmgBI CAC'GTC

Cuts at:      0      91    1140 
   Size:         91    1049
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     374     528    1140 
   Size:        374     154     612

  Fragments arranged by size: 

                 612     374     154
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     432     757     898    1140 
   Size:        432     325     141     242

  Fragments arranged by size: 

                 432     325     242     141
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373    1140 
   Size:        373     767
 
BsaAI yAC'GTr

Cuts at:      0     177    1140 
   Size:        177     963
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     376    1069    1088    1140 
   Size:        376     693      19      52

  Fragments arranged by size: 

                 693     376      52      19
 
BseYI C'CCAG_C

Cuts at:      0     691    1066    1140 
   Size:        691     375      74

  Fragments arranged by size: 

                 691     375      74
 
BsiHKAI G_wGCw'C

Cuts at:      0     523     884    1045    1140 
   Size:        523     361     161      95

  Fragments arranged by size: 

                 523     361     161      95
 
Bsp1286I G_dGCh'C

Cuts at:      0     234     326     523     884    1045    1140 
   Size:        234      92     197     361     161      95

  Fragments arranged by size: 

                 361     234     197     161      95      92
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     279     740     787    1140 
   Size:        279     461      47     353

  Fragments arranged by size: 

                 461     353     279      47
 
BsrBI CCG'CTC

Cuts at:      0     320    1140 
   Size:        320     820
 
BsrDI GCAATG_nn'

Cuts at:      0     440     587     855    1140 
   Size:        440     147     268     285

  Fragments arranged by size: 

                 440     285     268     147
 
BsrFI r'CCGG_y

Cuts at:      0     374     445    1085    1140 
   Size:        374      71     640      55

  Fragments arranged by size: 

                 640     374      71      55
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     291    1140 
   Size:        291     849
 
BstYI r'GATC_y

Cuts at:      0     111     848    1140 
   Size:        111     737     292

  Fragments arranged by size: 

                 737     292     111
 
BtsI GCAGTG_nn'

Cuts at:      0    1026    1140 
   Size:       1026     114
 
DraIII CAC_nnn'GTG

Cuts at:      0     639    1140 
   Size:        639     501
 
EaeI y'GGCC_r

Cuts at:      0     330     452     641    1101    1140 
   Size:        330     122     189     460      39

  Fragments arranged by size: 

                 460     330     189     122      39
 
EarI CTCTTCn'nnn_

Cuts at:      0     148     586     726    1140 
   Size:        148     438     140     414

  Fragments arranged by size: 

                 438     414     148     140
 
EcoICRI GAG'CTC

Cuts at:      0     882    1043    1140 
   Size:        882     161      97

  Fragments arranged by size: 

                 882     161      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     432     567     757     898    1140 
   Size:        432     135     190     141     242

  Fragments arranged by size: 

                 432     242     190     141     135
 
EcoRV GAT'ATC

Cuts at:      0     508    1140 
   Size:        508     632
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     319    1140 
   Size:        319     821
 
FspI TGC'GCA

Cuts at:      0     973    1140 
   Size:        973     167
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     292     324     818     850    1140 
   Size:        292      32     494      32     290

  Fragments arranged by size: 

                 494     292     290      32      32
 
MscI TGG'CCA

Cuts at:      0     643    1103    1140 
   Size:        643     460      37

  Fragments arranged by size: 

                 643     460      37
 
MslI CAynn'nnrTG

Cuts at:      0     865    1140 
   Size:        865     275
 
MspA1I CmG'CkG

Cuts at:      0     410     939    1066    1140 
   Size:        410     529     127      74

  Fragments arranged by size: 

                 529     410     127      74
 
NaeI GCC'GGC

Cuts at:      0     447    1087    1140 
   Size:        447     640      53

  Fragments arranged by size: 

                 640     447      53
 
NgoMIV G'CCGG_C

Cuts at:      0     445    1085    1140 
   Size:        445     640      55

  Fragments arranged by size: 

                 640     445      55
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     639    1140 
   Size:        639     501
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     334     366    1140 
   Size:        334      32     774

  Fragments arranged by size: 

                 774     334      32
 
PstI C_TGCA'G

Cuts at:      0    1065    1140 
   Size:       1065      75
 
PvuII CAG'CTG

Cuts at:      0     410     939    1066    1140 
   Size:        410     529     127      74

  Fragments arranged by size: 

                 529     410     127      74
 
SacI G_AGCT'C

Cuts at:      0     884    1045    1140 
   Size:        884     161      95

  Fragments arranged by size: 

                 884     161      95
 
SapI GCTCTTCn'nnn_

Cuts at:      0     148     586    1140 
   Size:        148     438     554

  Fragments arranged by size: 

                 554     438     148
 
SfcI C'TryA_G

Cuts at:      0     934    1061    1140 
   Size:        934     127      79

  Fragments arranged by size: 

                 934     127      79
 
SmlI C'TyrA_G

Cuts at:      0     388    1140 
   Size:        388     752
 
StyI C'CwwG_G

Cuts at:      0     248    1140 
   Size:        248     892
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      95    1140 
   Size:         95    1045
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      25      57    1140 
   Size:         25      32    1083

  Fragments arranged by size: 

                1083      32      25
 
XbaI T'CTAG_A

Cuts at:      0      72    1140 
   Size:         72    1068
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     246    1140 
   Size:        246     894
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AloI    AlwNI    BamHI
     BanI    BanII     BbsI     BglI    BglII Bme1580I    BmgBI     BmrI
     BpmI    BpuEI    BsaAI    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI    BstXI    BstYI     BtsI   DraIII     EaeI
     EarI  EcoICRI  Eco57MI    EcoRV     FalI     FspI    Hin4I     MscI
     MslI   MspA1I     NaeI   NgoMIV    PflMI     PpiI     PstI    PvuII
     SacI     SapI     SfcI     SmlI     StyI    TaqII     TstI     XbaI
     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI
     BmtI     BplI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI    EcoNI
 EcoO109I    EcoRI     FseI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XhoI
     XmaI     XmnI     ZraI
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