Restriction map for
pFN21AB8111
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII AvrII BbvCI BcgI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI HpaI
KpnI KspI MaeI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 404 CGCG CG^CG
AspI 96 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 296 CTCGAG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BamHI 1461 GGATCC G^GATCC
BbeI 181 GGCGCC GGCGC^C
BbrPI 1035 CACGTG CAC^GTG
BciVI 994 GGATAC GTATCC(6/5)
BglI 1142 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1035 CACGTG [CT]AC^GT[GA]
BsaWI 621 ACCGGA [AT]^CCGG[AT]
BsmI 119 GCATTC GAATGC(1/-1)
BsoBI 296 CTCGAG C^[CT]CG[GA]G
BsrBI 388 GAGCGG CCGCTC(-3/-3)
BssSI 934 CTCGTG CACGAG(-5/-1)
BstUI 404 CGCG CG^CG
DraI 1531 TTTAAA TTT^AAA
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 1302 GAAGAG CTCTTC(1/4)
EcoT22I 807 ATGCAT ATGCA^T
HaeII 181 GGCGCC [GA]GCGC^[CT]
HincII 1468 GTCAAC GT[CT]^[GA]AC
HindII 1468 GTCAAC GT[CT]^[GA]AC
HindIII 430 AAGCTT A^AGCTT
KasI 181 GGCGCC G^GCGCC
Ksp632I 1302 GAAGAG CTCTTC(1/4)
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MvnI 404 CGCG CG^CG
NarI 181 GGCGCC GG^CGCC
NcoI 149 CCATGG C^CATGG
NsiI 807 ATGCAT ATGCA^T
PaeR7I 296 CTCGAG C^TCGAG
PflFI 96 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 819 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1035 CACGTG CAC^GTG
PmlI 1035 CACGTG CAC^GTG
ScaI 581 AGTACT AGT^ACT
SfoI 181 GGCGCC GGC^GCC
SmlI 296 CTCGAG C^T[CT][GA]AG
Tth111I 96 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 819 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 296 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AocI 235 CCTGAGG CC^T[ACGT]AGG
919 CCTAAGG
ApaI 141 GGGCCC GGGCC^C
1380 GGGCCC
BanI 181 GGCGCC G^G[CT][GA]CC
1343 GGCACC
BbsI 1099 GAAGAC GAAGAC(2/6)
1164 GTCTTC
BcnI 178 CCCGG CC^[GC]GG
1650 CCCGG
BpuAI 1099 GAAGAC GAAGAC(2/6)
1164 GTCTTC
BsaI 535 GAGACC GGTCTC(1/5)
929 GAGACC
BseRI 1173 CTCCTC GAGGAG(10/8)
1176 CTCCTC
BsmAI 535 GAGAC GTCTC(1/5)
929 GAGAC
BsmFI 611 GGGAC GGGAC(10/14)
370 GTCCC
BsrFI 184 GCCGGC [GA]^CCGG[CT]
1086 GCCGGC
BstYI 1461 GGATCC [GA]^GATC[CT]
1613 AGATCC
Bsu36I 235 CCTGAGG CC^T[ACGT]AGG
919 CCTAAGG
Cfr10I 184 GCCGGC [GA]^CCGG[CT]
1086 GCCGGC
DsaI 149 CCATGG C^C[GA][CT]GG
281 CCACGG
Eco81I 235 CCTGAGG CC^T[ACGT]AGG
919 CCTAAGG
MflI 1461 GGATCC [GA]^GATC[CT]
1613 AGATCC
MslI 1155 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1595 CACACAAGTG
NaeI 184 GCCGGC GCC^GGC
1086 GCCGGC
NciI 178 CCCGG CC^[GC]GG
1650 CCCGG
NgoMIV 184 GCCGGC G^CCGGC
1086 GCCGGC
NspI 198 GCATGC [GA]CATG^[CT]
1154 GCATGC
PstI 161 CTGCAG CTGCA^G
193 CTGCAG
PvuII 19 CAGCTG CAG^CTG
1423 CAGCTG
SfcI 161 CTGCAG C^T[GA][CT]AG
193 CTGCAG
SphI 198 GCATGC GCATG^C
1154 GCATGC
Tsp45I 1033 GTCAC ^GT[GC]AC
1500 GTCAC
TspRI 159 CACTG CA[GC]TG[ACGT][ACGT]^
1385 CAGTG
XcmI 281 CCACGGTTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
716 CCAACTCATTCGTGG
XhoII 1461 GGATCC [GA]^GATC[CT]
1613 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AvaII BanII BbvI
BmyI BpmI BsaHI BsaJI BsgI
BsiYI BslI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI Tru9I TseI Tsp509I
TthHB8I