Restriction map for
pFN21AB8109
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaII AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cac8I CelII Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DsaI Eam1105I EarI Eco47III Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MwoI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 226 GTCTAC GT^[AC][GT]AC
AfaI 151 GTAC GT^AC
AvaI 140 CCCGGG C^[CT]CG[GA]G
BanI 426 GGCACC G^G[CT][GA]CC
BbvI 320 GCAGC GCAGC(8/12)
BfaI 239 CTAG C^TAG
BglII 170 AGATCT A^GATCT
BsaI 450 GAGACC GGTCTC(1/5)
BsmAI 450 GAGAC GTCTC(1/5)
BsmFI 343 GGGAC GGGAC(10/14)
BsoBI 140 CCCGGG C^[CT]CG[GA]G
BstYI 170 AGATCT [GA]^GATC[CT]
DdeI 213 CTTAG C^T[ACGT]AG
DrdI 360 GACCCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 111 CGGCCG [CT]^GGCC[GA]
EagI 111 CGGCCG C^GGCCG
EclXI 111 CGGCCG C^GGCCG
Eco52I 111 CGGCCG C^GGCCG
EcoNI 433 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeIII 112 GGCC GG^CC
HapII 141 CCGG C^CGG
HindIII 210 AAGCTT A^AGCTT
HpaII 141 CCGG C^CGG
HphI 98 TCACC GGTGA(8/7)
MaeI 239 CTAG C^TAG
MaeIII 19 GTTAC ^GT[ACGT]AC
MflI 170 AGATCT [GA]^GATC[CT]
MslI 177 CACTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 141 CCGG C^CGG
NspI 73 ACATGC [GA]CATG^[CT]
PflMI 120 CCACCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 10 GAGTC GAGTC(4/5)
PstI 364 CTGCAG CTGCA^G
PvuI 56 CGATCG CGAT^CG
RsaI 151 GTAC GT^AC
SfaNI 187 GATGC GCATC(5/9)
SfcI 364 CTGCAG C^T[GA][CT]AG
SmaI 140 CCCGGG CCC^GGG
TfiI 352 GATTC G^A[AT]TC
TseI 320 GCAGC G^C[AT]GC
TspRI 80 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 120 CCACCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 170 AGATCT [GA]^GATC[CT]
XmaCI 140 CCCGGG C^CCGGG
XmaI 140 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AluI 211 AGCT AG^CT
237 AGCT
BbsI 69 GAAGAC GAAGAC(2/6)
94 GTCTTC
BcnI 140 CCCGG CC^[GC]GG
141 CCGGG
BmyI 249 GGGCAC G[AGT]GC[ACT]^C
425 GGGCAC
BpuAI 69 GAAGAC GAAGAC(2/6)
94 GTCTTC
BsiYI 120 CCACCGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
433 CCTCCTTCAGG
BslI 120 CCACCGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
433 CCTCCTTCAGG
Bsp1286I 249 GGGCAC G[AGT]GC[ACT]^C
425 GGGCAC
BstF5I 182 GGATG GGATG(2/0)
186 GGATG
BstNI 24 CCAGG CC^[AT]GG
295 CCTGG
CfoI 15 GCGC GCG^C
444 GCGC
DpnII 57 GATC ^GATC
171 GATC
EcoRII 24 CCAGG ^CC[AT]GG
295 CCTGG
Fnu4HI 113 GCCGC GC^[ACGT]GC
320 GCAGC
FokI 182 GGATG GGATG(9/13)
186 GGATG
HhaI 15 GCGC GCG^C
444 GCGC
HinP1I 15 GCGC G^CGC
444 GCGC
HinfI 10 GAGTC G^A[ACGT]TC
352 GATTC
ItaI 113 GCCGC GC^[ACGT]GC
320 GCAGC
MboI 57 GATC ^GATC
171 GATC
MboII 69 GAAGA GAAGA(8/7)
95 TCTTC
MvaI 24 CCAGG CC^[AT]GG
295 CCTGG
NciI 140 CCCGG CC^[GC]GG
141 CCGGG
NdeII 57 GATC ^GATC
171 GATC
NlaIV 42 GGAGCC GG[ACGT]^[ACGT]CC
426 GGCACC
Sau3AI 57 GATC ^GATC
171 GATC
TaqI 59 TCGA T^CGA
303 TCGA
TthHB8I 59 TCGA T^CGA
303 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AccII AciI BsaJI BsiEI BssKI
BstUI MnlI MseI MvnI NlaIII
ScrFI Tru9I Tsp509I